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Buck-Wiese, Hagen; Andskog, Mona A; Nguyen, Nguyen P; Bligh, Margot; Gustafsson, Camilla; Asmala, Eero; Liebeke, Manuel; Vidal-Melgosa, Silvia; Hehemann, Jan-Hendrik (2022): Glycan-specific analyses conducted on water samples from incubation experiments with seagrass and algae sampled from Tvärminne Zoological Station, Hanko Finland, in August 2020 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.950868, In: Buck-Wiese, H et al. (2022): Incubation experiment of Fucus vesiculosus and Zostera marina specimen sampled from Tvärminne Zoological Station, Hanko Finland, in August 2020 [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.950878

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Published: 2022-11-16DOI registered: 2023-01-17

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Abstract:
This data was collected to investigate dissolved organic molecules that are released by seagrass and brown algae within the framework of an Assemble Plus Transnational Access project called Contributions of organic carbon from vegetated coastal ecosystems. The field experiments and water sample analyses were conducted in August 2020 at Tvärminne Zoological Station, Hanko Finland. The binary file glycan_data_ELISA contains results from antibody staining of seawater samples from algae incubations. Two fucoidan-targeting monoclonal antibodies were used, namely BAM1 and BAM2. Signal intensity indicating recognition of the substrate target in a sample by the antibody is given as relative intensity normalized to 1. The binary file glycan_data_HPAEC-PAD contains unprocessed results from high performance anion exchange chromatography which was used to quantify monosaccharides. Glycans in the samples were degraded to monosaccharides unspecifically using acid hydrolysis, or in a targeted manner using an enzyme cocktail specific to fucoidan. The binary file glycan_data_Fig2c contains data on fucoidan extraction from seawater using anion exchange chromatography. During the chromatography, two consecutive elution steps first with 2 M ammonium bicarbonate followed by 5 M sodium chloride were performed. The two eluted fractions were analyzed separately and results for monosaccharide content are given in this table. These glycan analyses were performed in 2020 and 2021 at the Max-Planck-Institute for marine microbiology in Bremen, Germany.
Keyword(s):
carbon dioxide removal; carbon sequestration; Dissolved Organic Carbon; fucose-containing sulfated polysaccharides; Fucus vesiculosus; Macroalgae; marine glycans
Supplement to:
Buck-Wiese, Hagen; Andskog, Mona A; Nguyen, Nguyen P; Bligh, Margot; Asmala, Eero; Vidal-Melgosa, Silvia; Liebeke, Manuel; Gustafsson, Camilla; Hehemann, Jan-Hendrik (2023): Fucoid brown algae inject fucoidan carbon into the ocean. Proceedings of the National Academy of Sciences of the United States of America, 120(1), e2210561119, https://doi.org/10.1073/pnas.2210561119
Funding:
Deutsche Forschungsgemeinschaft, Bonn (DFG), grant/award no. HE7217/1-1: DFG Emmy Noether program
Deutsche Forschungsgemeinschaft, Bonn (DFG), grant/award no. HE7217/5-1: DFG Heisenberg program
German Research Foundation (DFG), grant/award no. 390741603: EXC 2077: The Ocean Floor – Earth's Uncharted Interface
Coverage:
Latitude: 59.841190 * Longitude: 23.249530
Date/Time Start: 2020-08-18T14:00:00 * Date/Time End: 2020-08-18T14:00:00
Event(s):
Tvärmine_08-2020 * Latitude: 59.841190 * Longitude: 23.249530 * Date/Time: 2020-08-18T14:00:00 * Location: Hanko Peninsula, Finland
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Binary ObjectBinaryBuck-Wiese, Hagen
2File contentContentBuck-Wiese, Hagen
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
6 data points

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