/* DATA DESCRIPTION:
Citation:	Buck-Wiese, Hagen; Andskog, Mona A; Nguyen, Nguyen P; Bligh, Margot; Gustafsson, Camilla; Asmala, Eero; Liebeke, Manuel; Vidal-Melgosa, Silvia; Hehemann, Jan-Hendrik (2022): Incubation chamber volume and algal biomass, surface area and epiphyte biomass of Fucus vesiculosus specimen sampled at approx 1.5 m depth in the coastal Baltic Sea at Tvärminne Zoological Station, Hanko, Finland, in August 2020 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.950850, 
	In: Buck-Wiese, H et al. (2022): Incubation experiment of Fucus vesiculosus and Zostera marina specimen sampled from Tvärminne Zoological Station, Hanko Finland, in August 2020 [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.950878
Abstract:	For the algae incubations on August 18, 2020, transparent gas impermeable plastic vacuum bags (Packing24, PA/PE 20/70, 90my) with a single F. vesiculosus thallus, were closed with rubber bands around PVC end cap fitting of 12 cm diameter and attached to a bottom-anchored line at ~1 m depth. Thalli of ~50 cm length were detached close to the base by cutting the few millimeters thick, old stipe. Half of the incubations were covered with black bags to simulate dark conditions. Immediately prior to starting the incubations, water was sampled from the ambient water to determine initial conditions. At the end of the incubation, all algal tissue was collected. Water from within chambers was collected for analyses and the total volume measured. In the laboratory, the algal thallus was spread out across a cutting mat with a grid for surface area estimation. Epiphytes and epifauna were scraped off thalli using a razor blade, and collected separately from the algal biomass. All biomass was frozen and subsequently freeze-dried for 24 hours, and weighed to determine dry weight.
Keyword(s):	carbon dioxide removal; carbon sequestration; Dissolved Organic Carbon; fucose-containing sulfated polysaccharides; Fucus vesiculosus; Macroalgae; marine glycans
Supplement to:	Buck-Wiese, Hagen; Andskog, Mona A; Nguyen, Nguyen P; Bligh, Margot; Asmala, Eero; Vidal-Melgosa, Silvia; Liebeke, Manuel; Gustafsson, Camilla; Hehemann, Jan-Hendrik (2023): Fucoid brown algae inject fucoidan carbon into the ocean. Proceedings of the National Academy of Sciences of the United States of America, 120(1), e2210561119, https://doi.org/10.1073/pnas.2210561119
Project(s):	Contributions of organic carbon from vegetated coastal ecosystems (OC-VCE)
Funding:	Deutsche Forschungsgemeinschaft, Bonn (DFG) (URI: http://www.dfg.de/en/), grant/award no. HE7217/1-1: DFG Emmy Noether program
	Deutsche Forschungsgemeinschaft, Bonn (DFG) (URI: http://www.dfg.de/en/), grant/award no. HE7217/5-1: DFG Heisenberg program
	German Research Foundation (DFG) (URI: http://www.dfg.de/en/), grant/award no. 390741603: EXC 2077: The Ocean Floor – Earth's Uncharted Interface (URI: https://gepris.dfg.de/gepris/projekt/390741603)
Coverage:	LATITUDE: 59.841190 * LONGITUDE: 23.249530
	DATE/TIME START: 2020-08-18T14:00:00 * DATE/TIME END: 2020-08-18T14:00:00
Event(s):	Tvärmine_08-2020 * LATITUDE: 59.841190 * LONGITUDE: 23.249530 * DATE/TIME: 2020-08-18T14:00:00 * LOCATION: Hanko Peninsula, Finland
Parameter(s):	Chamber number (Chamber no) * PI: Buck-Wiese, Hagen (https://orcid.org/0000-0002-4807-5795, buckwies@usc.edu)
	Treatment: light condition (T:light) * PI: Buck-Wiese, Hagen (https://orcid.org/0000-0002-4807-5795, buckwies@usc.edu)
	Chamber volume [l] (Chamber vol) * PI: Buck-Wiese, Hagen (https://orcid.org/0000-0002-4807-5795, buckwies@usc.edu)
	Fucus vesiculosus, leaf area [cm**2] (F. vesiculosus LA) * PI: Buck-Wiese, Hagen (https://orcid.org/0000-0002-4807-5795, buckwies@usc.edu)
	Fucus vesiculosus, biomass as dry weight [g] (F. vesiculosus DM) * PI: Buck-Wiese, Hagen (https://orcid.org/0000-0002-4807-5795, buckwies@usc.edu)
	Epiphytes, biomass as dry weight [g] (Epiphyte DM) * PI: Buck-Wiese, Hagen (https://orcid.org/0000-0002-4807-5795, buckwies@usc.edu)
License:	Creative Commons Attribution 4.0 International (CC-BY-4.0) (URI: https://creativecommons.org/licenses/by/4.0/)
Status:	Curation Level: Enhanced curation (URI: https://wiki.pangaea.de/wiki/Curation_levels) * Processing Level: PANGAEA data processing level 2 (URI: https://wiki.pangaea.de/wiki/Processing_levels#PANGAEA_data_processing_level_2)
Size:	36 data points
*/
Chamber no	T:light	Chamber vol [l]	F. vesiculosus LA [cm**2]	F. vesiculosus DM [g]	Epiphyte DM [g]
1	light	6.80	1501.5	29.73	9.80
2	light	4.80	1052.8	23.45	5.15
3	light	4.76	720.4	16.50	15.18
4	dark	5.36	997.4	20.23	9.53
5	dark	5.40	1267.4	24.85	11.14
6	dark	5.26	1131.9	23.81	3.65