Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Liebner Susanne sliebner@gfz-potsdam.de 0000-0002-9389-7093 Jenner Anna-Kathrina anna.jenner@io-warnemuende.de Knorr Klaus-Holger kh.knorr@uni-muenster.de 0000-0003-4175-0214 Unger Viktoria Koebsch Franziska franziska.koebsch@uni-greifswald.de Racasa Erwin Don erwin.racasa@uni-rostock.de 0000-0002-9300-1744 Yang Sizhong 0000-0001-8417-593X Böttcher Michael Ernst michael.boettcher@io-warnemuende.de 0000-0002-8877-0303 Janssen Manon manon.janssen@uni-rostock.d 0000-0001-7249-8870 Kallmeyer Jens kallm@gfz-potsdam.de 0000-0002-6440-1140 Otto Denise Schmiedinger Iris iris.schmiedinger@io-warnemuende.de Winski Lucas Jurasinski Gerald gerald.jurasinski@uni-greifswald.de 0000-0002-6248-9388 2022 Effects of a brackish water inflow on peat pore water variables and methane-cycling microbial community abundances in a freshwater rewetted coastal fen dataset https://doi.org/10.1594/PANGAEA.949824 2022-10-21T07:16:25 The rewetting of peatlands is a promising measure to mitigate greenhouse gas (GHG) emissions by preventing the further mineralization of the peat soil through aeration. In coastal peatland, the rewetting with brackish water can increase the GHG mitigation potential by the introduction of sulfate, a terminal electron acceptor (TEA). Sulfate is known to lower the CH4 production and thus, its emission by favoring the growth of sulfate-reducers, which outcompete methanogens for substrate. The data contain porewater variables such as pH, electrical conductivity (EC) and sulfate, chloride, dissolved CO2 and CH4 concentrations, as well as absolute abundances of methane- and sulfate-cycling microbial communities. The data were collected in spring and autumn 2019 after a storm surge with brackish water inflow in January 2019. Field sampling was conducted in the nature reserve Heiligensee and Hütelmoor in North-East Germany, close to the Southern Baltic Sea coast. We took peat cores using a Russian peat corer in addition to pore water diffusion samplers and plastic liners (length: 60cm; inner diameter 10 cm) at four locations along a transect from further inland towards the Baltic Sea. We wanted to compare the soil and pore water geochemistry as well as the microbial communities after the brackish water inflow to the common freshwater rewetting state. Pore water was extracted using pore water suction samplers in the lab and environmental variables were quantified with an ICP. Microbial samples were sampled from the peat core using sterile equipment. We used quantitative polymerase chain reaction (qPCR) to characterize pools of DNA and cDNA targeting total and putatively active bacteria and archaea. qPCR was performed on key functional genes of methane production (mcrA), aerobic methane oxidation (pmoA) and sulfate reduction (dsrB) in addition to the 16S rRNA gene for the absolute abundance of total prokaryotes. Furthermore, we retrieved soil plugs to determine the concentrations and isotopic signatures of dissolved trace gases (CO2/DIC and CH4) in the pore water. Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Liebner Susanne sliebner@gfz-potsdam.de 0000-0002-9389-7093 Jenner Anna-Kathrina anna.jenner@io-warnemuende.de Knorr Klaus-Holger kh.knorr@uni-muenster.de 0000-0003-4175-0214 Unger Viktoria Koebsch Franziska franziska.koebsch@uni-greifswald.de Racasa Erwin Don erwin.racasa@uni-rostock.de 0000-0002-9300-1744 Yang Sizhong 0000-0001-8417-593X Böttcher Michael Ernst michael.boettcher@io-warnemuende.de 0000-0002-8877-0303 Janssen Manon manon.janssen@uni-rostock.d 0000-0001-7249-8870 Kallmeyer Jens kallm@gfz-potsdam.de 0000-0002-6440-1140 Otto Denise Schmiedinger Iris iris.schmiedinger@io-warnemuende.de Winski Lucas Jurasinski Gerald gerald.jurasinski@uni-greifswald.de 0000-0002-6248-9388 2022 Effects of brackish water inflow on methane-cycling microbial communities in a freshwater rewetted coastal fen journal article Biogeosciences 19(15) https://doi.org/10.5194/bg-19-3625-2022 3625-3648 12.1749 12.18572 54.21095 54.21806 12.179464 54.214454 2019-05-16T00:00:00 2019-12-02T00:00:00 0.0 0.45 point TRANSCOAST_Hutelmoor_HC1 54.21095 12.18572 Northeastern Germany 2019-11-28T00:00:00 Russian peat corer RPC TRANSCOAST_Hutelmoor_HC2-1 54.21341 12.1812 Northeastern Germany 2019-05-16T00:00:00 Russian peat corer RPC TRANSCOAST_Hutelmoor_HC2-2 54.213 12.1802 Northeastern Germany 2019-11-28T00:00:00 Russian peat corer RPC TRANSCOAST_Hutelmoor_HC3 54.21685 12.1753 Northeastern Germany 2019-12-02T00:00:00 Russian peat corer RPC TRANSCOAST_Hutelmoor_HC4 54.21806 12.1749 Northeastern Germany 2019-12-02T00:00:00 Russian peat corer RPC 926 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Event label Event Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Event Date/Time of event Date/Time Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Date/Time Latitude of event Latitude Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Latitude Longitude of event Longitude Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Longitude Sample ID Sample ID Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Sample ID Sample comment Sample comment Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Sampling_time - Post-inflow spring or autumn 2019 Sample comment (Sampling_time - Post-inflow s...) Sample comment Sample comment Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Sampling locations along the transect : HC1, HC2, HC3, HC4 Sample comment (Sampling locations along the ...) Depth, soil, minimum Depth soil min m depth http://purl.obolibrary.org/obo/PATO_0001595 soil http://purl.obolibrary.org/obo/ENVO_00001998 minimum Length L http://dbpedia.org/resource/Length Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Depth soil min [m] Depth, soil, maximum Depth soil max m depth http://purl.obolibrary.org/obo/PATO_0001595 soil http://purl.obolibrary.org/obo/ENVO_00001998 maximum Length L http://dbpedia.org/resource/Length Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Depth soil max [m] DEPTH, soil Depth soil m depth http://purl.obolibrary.org/obo/PATO_0001595 soil http://purl.obolibrary.org/obo/ENVO_00001998 Length L http://dbpedia.org/resource/Length Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Depth soil [m] Depth, relative Depth rel m depth http://purl.obolibrary.org/obo/PATO_0001595 relative Length L http://dbpedia.org/resource/Length Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Anchor - Specific depth within the depth zone Depth rel [m] (Anchor - Specific depth withi...) Replicate Repl Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Biological replicates were taken for microbial analysis including pore water trace gas analysis Repl (Biological replicates were ta...) Type of study Study type type Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de DNA-type - were microbial gene abundance data retrieved from DNA or cDNA Study type (DNA-type - were microbial gen...) pH pH pH meter Chemical property sensors pH sensors http://vocab.nerc.ac.uk/collection/L05/current/355/ Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de pH (pH meter) Conductivity, electrical EC mS/m conductivity http://purl.obolibrary.org/obo/PATO_0001585 Refractometer Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de EC [mS/m] (Refractometer) Sulfate [SO4]2- mmol/l sulfate http://purl.obolibrary.org/obo/CHEBI_16189 Molar Concentration Ion coupled plasma spectrometry (ICP) Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Pore water concentration [SO4]2- [mmol/l] (Pore water concentration, Ion...) Chloride Cl- mmol/l chloride http://purl.obolibrary.org/obo/CHEBI_17996 Molar Concentration Ion coupled plasma spectrometry (ICP) Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Pore water concentration Cl- [mmol/l] (Pore water concentration, Ion...) Carbon dioxide CO2 µmol/l carbon dioxide http://purl.obolibrary.org/obo/CHEBI_16526 Molar Concentration Gas chromatograph GASC Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Pore water concentration CO2 [µmol/l] (Pore water concentration, Gas...) Methane CH4 µmol/l methane http://purl.obolibrary.org/obo/CHEBI_16183 Molar Concentration Gas chromatograph GASC Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Pore water concentration CH4 [µmol/l] (Pore water concentration, Gas...) δ13C, dissolved inorganic carbon δ13C DIC ‰ PDB δ13C http://en.wikipedia.org/wiki/List_of_elements_by_stability_of_isotopes dissolved inorganic carbon DIC Cavity ring-down spectroscopy CRDS cavity enhanced absorption spectrometers http://vocab.nerc.ac.uk/collection/L05/current/LAB38/ Chromatographs & spectrometers Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Pore water δ13C DIC [‰ PDB] (Pore water, Cavity ring-down ...) δ13C, methane δ13C CH4 ‰ PDB δ13C http://en.wikipedia.org/wiki/List_of_elements_by_stability_of_isotopes methane http://purl.obolibrary.org/obo/CHEBI_16183 Cavity ring-down spectroscopy CRDS cavity enhanced absorption spectrometers http://vocab.nerc.ac.uk/collection/L05/current/LAB38/ Chromatographs & spectrometers Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Pore water δ13C CH4 [‰ PDB] (Pore water, Cavity ring-down ...) Gene copies per gram sediment GCN per sed #/g Real-time quantitative polymerase chain reaction (qPCR) https://en.wikipedia.org/wiki/Real-time_polymerase_chain_reaction Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de X16S_rRNA: total prokaryotes GCN per sed [#/g] (X16S_rRNA: total prokaryotes,...) Gene copies per gram sediment GCN per sed #/g Real-time quantitative polymerase chain reaction (qPCR) https://en.wikipedia.org/wiki/Real-time_polymerase_chain_reaction Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de mcrA: methanogenic archaea GCN per sed [#/g] (mcrA: methanogenic archaea, R...) Gene copies per gram sediment GCN per sed #/g Real-time quantitative polymerase chain reaction (qPCR) https://en.wikipedia.org/wiki/Real-time_polymerase_chain_reaction Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de pmoA: methanotrophic bacteria GCN per sed [#/g] (pmoA: methanotrophic bacteria...) Gene copies per gram sediment GCN per sed #/g Real-time quantitative polymerase chain reaction (qPCR) https://en.wikipedia.org/wiki/Real-time_polymerase_chain_reaction Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de drsB: sulfate-reducing bacteria GCN per sed [#/g] (drsB: sulfate-reducing bacter...) Device type Device Gutekunst Cordula Nina cordula.gutekunst@uni-greifswald.de Pore water device Device (Pore water device) CurationLevelC Enhanced curation https://wiki.pangaea.de/wiki/Curation_levels citable Coastal peatland rewetting methanogens methanotrophs pore water geochemistry sulfate-reducing bacteria author26996 author28867 author31144 author57691 author68868 author70167 author70169 author70170 author70172 author75564 author88916 author94009 author94010 author94011 author94012 curlevel30 event3028251 event3028921 event3028922 event3028923 event3028924 geocode143506 geocode1599 geocode1600 geocode1601 journal2146 license21 method11560 method12235 method6805 method6970 method7384 method8290 method9551 param102721 param115236 param128278 param129269 param143511 param143512 param16065 param18011 param181349 param185520 param189911 param29869 param50 param54 param764 param770 param78085 pi94009 ref115745 term1073089 term1073131 term1073162 term1073174 term1073219 term1073490 term1073620 term2299447 term2299532 term38492 term38526 term40625 term41019 term42421 term43863 term44030 term47201 term49506 term51336 term67625 term68027 term71020 term71552 term71553 term71554 topotype2