de.pangaea.dataset949824 und dataset PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact 2026-06-26T09:32:10Z https://doi.pangaea.de/10.1594/PANGAEA.949824 <gco:CharacterString>Effects of a brackish water inflow on peat pore water variables and methane-cycling microbial community abundances in a freshwater rewetted coastal fen</gco:CharacterString> 2022-10-21T07:16:25 publication https://doi.org/10.1594/PANGAEA.949824 Gutekunst, Cordula Nina
cordula.gutekunst@uni-greifswald.de
author Liebner, Susanne
sliebner@gfz-potsdam.de
https://orcid.org/0000-0002-9389-7093 information author Jenner, Anna-Kathrina
anna.jenner@io-warnemuende.de
author Knorr, Klaus-Holger
kh.knorr@uni-muenster.de
https://orcid.org/0000-0003-4175-0214 information author Unger, Viktoria author Koebsch, Franziska
franziska.koebsch@uni-greifswald.de
author Racasa, Erwin Don
erwin.racasa@uni-rostock.de
https://orcid.org/0000-0002-9300-1744 information author Yang, Sizhong https://orcid.org/0000-0001-8417-593X information author Böttcher, Michael Ernst
michael.boettcher@io-warnemuende.de
https://orcid.org/0000-0002-8877-0303 information author Janssen, Manon
manon.janssen@uni-rostock.d
https://orcid.org/0000-0001-7249-8870 information author Kallmeyer, Jens
kallm@gfz-potsdam.de
https://orcid.org/0000-0002-6440-1140 information author Otto, Denise author Schmiedinger, Iris
iris.schmiedinger@io-warnemuende.de
author Winski, Lucas author Jurasinski, Gerald
gerald.jurasinski@uni-greifswald.de
https://orcid.org/0000-0002-6248-9388 information author PANGAEA
info@pangaea.de
https://www.pangaea.de/ information publisher tableDigital The rewetting of peatlands is a promising measure to mitigate greenhouse gas (GHG) emissions by preventing the further mineralization of the peat soil through aeration. In coastal peatland, the rewetting with brackish water can increase the GHG mitigation potential by the introduction of sulfate, a terminal electron acceptor (TEA). Sulfate is known to lower the CH4 production and thus, its emission by favoring the growth of sulfate-reducers, which outcompete methanogens for substrate. The data contain porewater variables such as pH, electrical conductivity (EC) and sulfate, chloride, dissolved CO2 and CH4 concentrations, as well as absolute abundances of methane- and sulfate-cycling microbial communities. The data were collected in spring and autumn 2019 after a storm surge with brackish water inflow in January 2019. Field sampling was conducted in the nature reserve Heiligensee and Hütelmoor in North-East Germany, close to the Southern Baltic Sea coast. We took peat cores using a Russian peat corer in addition to pore water diffusion samplers and plastic liners (length: 60cm; inner diameter 10 cm) at four locations along a transect from further inland towards the Baltic Sea. We wanted to compare the soil and pore water geochemistry as well as the microbial communities after the brackish water inflow to the common freshwater rewetting state. Pore water was extracted using pore water suction samplers in the lab and environmental variables were quantified with an ICP. Microbial samples were sampled from the peat core using sterile equipment. We used quantitative polymerase chain reaction (qPCR) to characterize pools of DNA and cDNA targeting total and putatively active bacteria and archaea. qPCR was performed on key functional genes of methane production (mcrA), aerobic methane oxidation (pmoA) and sulfate reduction (dsrB) in addition to the 16S rRNA gene for the absolute abundance of total prokaryotes. Furthermore, we retrieved soil plugs to determine the concentrations and isotopic signatures of dissolved trace gases (CO2/DIC and CH4) in the pore water. completed Gutekunst, Cordula Nina
cordula.gutekunst@uni-greifswald.de
principalInvestigator Coastal peatland rewetting methanogens methanotrophs pore water geochemistry sulfate-reducing bacteria theme <gco:CharacterString>PANGAEA Keyword List</gco:CharacterString> 1995-01-01 creation PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact copyright copyright CC-BY-4.0: Creative Commons Attribution 4.0 International textTable und geoscientificInformation 12.1749 12.18572 54.21095 54.21806 2019-05-16T00:00:00 2019-12-02T00:00:00 Event label thematicClassification Date/Time of event thematicClassification Latitude of event thematicClassification Longitude of event thematicClassification Sample ID thematicClassification Sample comment thematicClassification Sampling_time - Post-inflow spring or autumn 2019 Sample comment thematicClassification Sampling locations along the transect : HC1, HC2, HC3, HC4 Depth, soil, minimum physicalMeasurement m m Depth, soil, maximum physicalMeasurement m m DEPTH, soil physicalMeasurement m m Depth, relative physicalMeasurement Anchor - Specific depth within the depth zone m m Replicate thematicClassification Biological replicates were taken for microbial analysis including pore water trace gas analysis Type of study thematicClassification DNA-type - were microbial gene abundance data retrieved from DNA or cDNA pH thematicClassification pH meter Conductivity, electrical physicalMeasurement Refractometer mS/m mS/m Sulfate physicalMeasurement Ion coupled plasma spectrometry (ICP); Pore water concentration mmol/l mmol/l Chloride physicalMeasurement Ion coupled plasma spectrometry (ICP); Pore water concentration mmol/l mmol/l Carbon dioxide physicalMeasurement Gas chromatograph; Pore water concentration µmol/l µmol/l Methane physicalMeasurement Gas chromatograph; Pore water concentration µmol/l µmol/l δ13C, dissolved inorganic carbon physicalMeasurement Cavity ring-down spectroscopy; Pore water ‰ PDB ‰ PDB δ13C, methane physicalMeasurement Cavity ring-down spectroscopy; Pore water ‰ PDB ‰ PDB Gene copies per gram sediment physicalMeasurement Real-time quantitative polymerase chain reaction (qPCR); X16S_rRNA: total prokaryotes #/g #/g Gene copies per gram sediment physicalMeasurement Real-time quantitative polymerase chain reaction (qPCR); mcrA: methanogenic archaea #/g #/g Gene copies per gram sediment physicalMeasurement Real-time quantitative polymerase chain reaction (qPCR); pmoA: methanotrophic bacteria #/g #/g Gene copies per gram sediment physicalMeasurement Real-time quantitative polymerase chain reaction (qPCR); drsB: sulfate-reducing bacteria #/g #/g Device type thematicClassification Pore water device text/tab-separated-values unknown https://doi.pangaea.de/10.1594/PANGAEA.949824?format=textfile download dataset The data set was checked for completeness, correctness, and consistency of metainformation. Validity of used methods was checked and - if applicable - precision and range of data. EVENT LABEL: TRANSCOAST_Hutelmoor_HC1 * LATITUDE: 54.210950 * LONGITUDE: 12.185720 * DATE/TIME: 2019-11-28T00:00:00 * LOCATION: Northeastern Germany * METHOD/DEVICE: Russian peat corer 2019-11-28T00:00:00 EVENT LABEL: TRANSCOAST_Hutelmoor_HC2-1 * LATITUDE: 54.213410 * LONGITUDE: 12.181200 * DATE/TIME: 2019-05-16T00:00:00 * LOCATION: Northeastern Germany * METHOD/DEVICE: Russian peat corer 2019-05-16T00:00:00 EVENT LABEL: TRANSCOAST_Hutelmoor_HC2-2 * LATITUDE: 54.213000 * LONGITUDE: 12.180200 * DATE/TIME: 2019-11-28T00:00:00 * LOCATION: Northeastern Germany * METHOD/DEVICE: Russian peat corer 2019-11-28T00:00:00 EVENT LABEL: TRANSCOAST_Hutelmoor_HC3 * LATITUDE: 54.216850 * LONGITUDE: 12.175300 * DATE/TIME: 2019-12-02T00:00:00 * LOCATION: Northeastern Germany * METHOD/DEVICE: Russian peat corer 2019-12-02T00:00:00 EVENT LABEL: TRANSCOAST_Hutelmoor_HC4 * LATITUDE: 54.218060 * LONGITUDE: 12.174900 * DATE/TIME: 2019-12-02T00:00:00 * LOCATION: Northeastern Germany * METHOD/DEVICE: Russian peat corer 2019-12-02T00:00:00 Related to <gco:CharacterString>Effects of brackish water inflow on methane-cycling microbial communities in a freshwater rewetted coastal fen</gco:CharacterString> 2022-01-01 publication https://doi.org/10.5194/bg-19-3625-2022 Gutekunst, Cordula Nina
cordula.gutekunst@uni-greifswald.de
author Liebner, Susanne
sliebner@gfz-potsdam.de
https://orcid.org/0000-0002-9389-7093 information author Jenner, Anna-Kathrina
anna.jenner@io-warnemuende.de
author Knorr, Klaus-Holger
kh.knorr@uni-muenster.de
https://orcid.org/0000-0003-4175-0214 information author Unger, Viktoria author Koebsch, Franziska
franziska.koebsch@uni-greifswald.de
author Racasa, Erwin Don
erwin.racasa@uni-rostock.de
https://orcid.org/0000-0002-9300-1744 information author Yang, Sizhong https://orcid.org/0000-0001-8417-593X information author Böttcher, Michael Ernst
michael.boettcher@io-warnemuende.de
https://orcid.org/0000-0002-8877-0303 information author Janssen, Manon
manon.janssen@uni-rostock.d
https://orcid.org/0000-0001-7249-8870 information author Kallmeyer, Jens
kallm@gfz-potsdam.de
https://orcid.org/0000-0002-6440-1140 information author Otto, Denise author Schmiedinger, Iris
iris.schmiedinger@io-warnemuende.de
author Winski, Lucas author Jurasinski, Gerald
gerald.jurasinski@uni-greifswald.de
https://orcid.org/0000-0002-6248-9388 information author documentDigital Biogeosciences 19(15) 3625-3648