Citation:

PANGAEA.949740

Name: Seasonal and inter-annual variability in the heatwave tolerance of the kelp Saccharina latissima
Published: 2022-10-17
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Acclimation; Climate change; Fv/Fm; growth; Laboratory experiment; pigments; Saccharina latissima; Survival; Temperature tolerance

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Abstract:

Temperature is a major driver for the geographical distribution of organisms, such as the foundation kelp species Saccharina latissima. Globally rising sea surface temperatures and intensification of marine heatwaves have already led to local loss of kelp populations. We investigated temporal variations in the thermal susceptibility of S. latissima. Therefore, we assessed the stress responses of field sporophytes sampled from Helgoland (German Bight) to an experimental heat wave scenario in June 2018, August 2018, and August 2019. The experiment in June 2018 was conducted by Diehl et al. (2021a) and the respective dataset (Diehl et al. 2021b, https://doi.org/10.1594/PANGAEA.931637) was re-evaluated for this study. Treatment temperatures (18, 20, 22, 24 °C) were based on 18 °C summer mean sea surface temperature on Helgoland as control, and Δ+2, Δ+4, Δ+6 °C as temperature-amplitude treatments, mimicking marine heatwaves. After a three-days wound healing phase, seven days of temperature acclimation (day 0-7) and seven days of temperature treatment (day 8-14) followed. The survival, growth and maximum photosynthetic quantum yield (Fv/Fm; June 2018/August 2018: ImagingPAM, Walz Imaging PAM Maxi Version M-series; August 2019: Portable Chlorophyll Fluorometer PAM-2100, Heinz Walz GmbH, Effeltrich, Germany) were measured on day 0 and day 14. To highlight changes as response to the experimental heat wave, physiological parameters were shown as percentage of the initial values. Absolute concentrations of pigments were analyzed using a HPLC. Afterwards, accessory pigment (Acc) and xanthophyll cycle pigment (VAZ) concentration, as well as the de-epoxidation state of the xanthophyll cycle (DPS) and ratios were calculated.

Keyword(s):

Acclimation; Climate change; Fv/Fm; growth; Laboratory experiment; pigments; Saccharina latissima; Survival; Temperature tolerance

Supplement to:

Niedzwiedz, Sarina; Diehl, Nora; Fischer, Philipp; Bischof, Kai (2022): Seasonal and inter‐annual variability in the heatwave tolerance of the kelp Saccharina latissima (Laminariales, Phaeophyceae). Phycological Research, 70(4), 212-222, https://doi.org/10.1111/pre.12501

Related to:

Diehl, Nora; Roleda, Michael Y; Bartsch, Inka; Karsten, Ulf; Bischof, Kai (2021): Summer Heatwave Impacts on the European Kelp Saccharina latissima Across Its Latitudinal Distribution Gradient. Frontiers in Marine Science, 8, https://doi.org/10.3389/fmars.2021.695821

Diehl, Nora; Roleda, Michael Y; Bartsch, Inka; Karsten, Ulf; Bischof, Kai (2021): Summer heatwave impacts on the European kelp Saccharina latissima across its latitudinal distribution gradient – raw data to temperature-amplitude experiment with field material from five different locations. PANGAEA, https://doi.org/10.1594/PANGAEA.931637

Further details:

Colombo-Pallotta, María Florencia; García-Mendoza, Ernesto; Ladah, Lydia B (2006): Photosynthetic performance, light absorption, and pigment composition of Macrocystis pyrifera (Laminariales, Phaeophyceae) blades from different depths. Journal of Phycology, 42(6), 1225-1234, https://doi.org/10.1111/j.1529-8817.2006.00287.x

Coverage:

Median Latitude: 54.186090 * Median Longitude: 7.908310 * South-bound Latitude: 54.181670 * West-bound Longitude: 7.903300 * North-bound Latitude: 54.188300 * East-bound Longitude: 7.918330

Date/Time Start: 2018-07-03T06:00:00 * Date/Time End: 2019-09-26T06:00:00

Event(s):

Helgoland_Slatissima_2018a * Latitude: 54.181670 * Longitude: 7.918330 * Date/Time: 2018-06-26T00:00:00 * Location: Helgoland, North Sea * Method/Device: Sampling by diver (DIVER) * Comment: Water depth 3-5 m

Helgoland_Slatissima_2018b * Latitude: 54.188300 * Longitude: 7.903300 * Date/Time: 2018-08-23T00:00:00 * Location: Helgoland, North Sea * Method/Device: Sampling by diver (DIVER) * Comment: Water depth 3-5 m

Helgoland_Slatissima_2019 * Latitude: 54.188300 * Longitude: 7.903300 * Date/Time: 2019-08-21T00:00:00 * Location: Helgoland, North Sea * Method/Device: Sampling by diver (DIVER) * Comment: Water depth 3-5 m

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Niedzwiedz, Sarina
2	Type of study	Study type		Niedzwiedz, Sarina
3	Family	Family		Niedzwiedz, Sarina
4	Species	Species		Niedzwiedz, Sarina
5	Genus, unique identification (URI)	Genus UID (URI)		Niedzwiedz, Sarina
6	Genus, unique identification (Semantic URI)	Genus UID (Semantic URI)		Niedzwiedz, Sarina
7	DATE/TIME	Date/Time		Niedzwiedz, Sarina		Geocode – experiment
8	Experiment day	Exp day	day	Niedzwiedz, Sarina
9	Temperature, water	Temp	°C	Niedzwiedz, Sarina
10	Replicate	Repl		Niedzwiedz, Sarina
11	Survival	Surv	#	Niedzwiedz, Sarina	Visual observation	discs
12	Survival	Survival	%	Niedzwiedz, Sarina	Calculated	discs
13	Size, algal disc	Size algal disc	cm²	Niedzwiedz, Sarina	Measured using software ImageJ
14	Area, algal disc, relative	Area algal disc rel	%	Niedzwiedz, Sarina	Calculated
15	Maximum photochemical quantum yield of photosystem II	Fv/Fm		Niedzwiedz, Sarina	Pulse amplitude modulation (PAM) fluorometry
16	Maximum photochemical quantum yield of photosystem II, relative	Fv/Fm rel	%	Niedzwiedz, Sarina	Calculated
17	Chlorophyll c, per dry mass	Chl c dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
18	Fucoxanthin, per dry mass	Fuco dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
19	Violaxanthin, per dry mass	Viola dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
20	Antheraxanthin, per dry mass	Anthera dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
21	Zeaxanthin, per dry mass	Zea dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
22	Chlorophyll a, per dry mass	Chl a dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
23	Pigments, accessory, per dry mass	Pigm acc dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
24	Pigments, accessory/chlorophyll a ratio	Pigm acc/chl a		Niedzwiedz, Sarina	Calculated
25	Violaxanthin + Antheraxanthin + Zeaxanthin, per dry mass	VAZ dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
26	Pigments, violaxanthin-xanthophyll-cycle/chlorophyll a ratio	VAZ/Chl a		Niedzwiedz, Sarina	Calculated
27	De-expoxidation state of xanthophyll cycle	DPS		Niedzwiedz, Sarina	after Colombo-Pallotta et al. (2006)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1683 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 Study type	3 Family	4 Species	5 Genus UID (URI)	6 Genus UID (Semantic URI)	7 Date/Time	8 Exp day [day]	9 Temp [°C]	10 Repl	11 Surv [#]	12 Survival [%]	13 Size algal disc [cm²]	14 Area algal disc rel [%]	15 Fv/Fm	16 Fv/Fm rel [%]	17 Chl c dm [µg/g]	18 Fuco dm [µg/g]	19 Viola dm [µg/g]	20 Anthera dm [µg/g]	21 Zea dm [µg/g]	22 Chl a dm [µg/g]	23 Pigm acc dm [µg/g]	24 Pigm acc/chl a	25 VAZ dm [µg/g]	26 VAZ/Chl a	27 DPS
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-03T06:00:00	0	18	1	5		3.80		0.64
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-03T06:00:00	0	18	2	5		3.80		0.64
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-03T06:00:00	0	18	3	5		3.80		0.64
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-03T06:00:00	0	18	4	5		3.80		0.64
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-03T06:00:00	0	20	1	5		3.80		0.68
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-03T06:00:00	0	20	2	5		3.80		0.68
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-03T06:00:00	0	20	3	5		3.80		0.68
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-03T06:00:00	0	20	4	5		3.80		0.68
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-03T06:00:00	0	22	1	5		3.80		0.65
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-03T06:00:00	0	22	2	5		3.80		0.65
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-03T06:00:00	0	22	3	5		3.80		0.65
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-03T06:00:00	0	22	4	5		3.80		0.65
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-03T06:00:00	0	24	1	5		3.80		0.66
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-03T06:00:00	0	24	2	5		3.80		0.66
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-03T06:00:00	0	24	3	5		3.80		0.66
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-03T06:00:00	0	24	4	5		3.80		0.66
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-17T06:00:00	14	18	1	5	100.00	4.75	124.89	0.71	110.94	197.80	656.57	49.45	1.86	0.29	1152.92	854.37	0.74	51.59	0.04	0.02
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-17T06:00:00	14	18	2	5	100.00	4.39	115.60	0.69	108.31	196.50	640.62	62.94	3.53	1.38	1176.85	837.12	0.71	67.85	0.06	0.05
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-17T06:00:00	14	18	3	5	100.00	4.65	122.35	0.66	102.44	272.99	923.19	78.70	8.89	6.05	1572.46	1196.18	0.76	93.64	0.06	0.11
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-17T06:00:00	14	18	4	5	100.00	4.46	117.37	0.70	109.97	191.96	600.36	54.53	1.95	0.83	1114.06	792.32	0.71	57.31	0.05	0.03
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-17T06:00:00	14	20	1	5	100.00	4.43	116.46	0.64	94.67	207.87	707.62	47.65	2.49	0.50	1276.61	915.49	0.72	50.64	0.04	0.03
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-17T06:00:00	14	20	2	4	80.00	4.75	125.00	0.66	97.37	177.51	563.97	62.50	3.60	0.53	1050.37	741.47	0.71	66.63	0.06	0.04
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-17T06:00:00	14	20	3	5	100.00	4.39	115.40	0.64	95.35	229.81	756.41	63.72	4.78	0.77	1429.58	986.22	0.69	69.27	0.05	0.05
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-17T06:00:00	14	20	4	5	100.00	4.43	116.47	0.64	94.22	173.24	574.64	62.31	3.97	1.23	1090.11	747.88	0.69	67.52	0.06	0.05
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-17T06:00:00	14	22	1	5	100.00	4.43	116.50	0.56	85.42	213.77	748.08	38.37	7.45	32.05	1353.27	961.85	0.71	77.88	0.06	0.46
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-17T06:00:00	14	22	2	5	100.00	4.33	113.81	0.63	96.20	179.19	646.49	73.24	6.78	2.09	1265.31	825.68	0.65	82.12	0.06	0.07
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-17T06:00:00	14	22	3	5	100.00	4.42	116.29	0.66	101.23	169.27	608.12	60.73	6.04	1.34	1172.45	777.39	0.66	68.11	0.06	0.06
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-17T06:00:00	14	22	4	4	80.00	4.54	119.39	0.57	87.75	181.80	623.51	67.25	6.13	1.57	1197.36	805.31	0.67	74.95	0.06	0.06
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-17T06:00:00	14	24	1	3	60.00	4.44	116.84	0.50	76.50	149.46	571.56	33.67	21.41	36.77	1053.32	721.02	0.68	91.86	0.09	0.52
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-17T06:00:00	14	24	2	3	60.00	4.62	121.64	0.41	62.26	77.82	306.44	19.24	11.69	41.62	579.39	384.26	0.66	72.55	0.13	0.65
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-17T06:00:00	14	24	3	3	60.00	4.46	117.20	0.54	81.66	153.41	594.84	34.94	27.79	78.87	1145.92	748.25	0.65	141.60	0.12	0.66
Helgoland_Slatissima_2018a	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-07-17T06:00:00	14	24	4	5	100.00	4.34	114.27	0.41	62.37	133.13	515.84	25.44	22.01	79.73	1060.39	648.97	0.61	127.18	0.12	0.71
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-08-27T06:00:00	0	18	1	10		4.05		0.65		93.94	352.15	18.19	2.55	0.42	550.83	446.10	0.81	21.16	0.04	0.08
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-08-27T06:00:00	0	18	2	10		4.10		0.63		224.87	914.72	123.49	3.64	0.57	2092.16	1139.59	0.54	127.70	0.06	0.02
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-08-27T06:00:00	0	18	3	10		4.09		0.64		113.05	390.65	25.19	2.10	0.96	678.00	503.70	0.74	28.24	0.04	0.07
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-08-27T06:00:00	0	18	4	10		4.04		0.63		116.94	406.82	17.69	1.71	0.78	594.32	523.75	0.88	20.19	0.03	0.08
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-08-27T06:00:00	0	20	1	10		3.93		0.63
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-08-27T06:00:00	0	20	2	10		4.03		0.66
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-08-27T06:00:00	0	20	3	10		4.02		0.66
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-08-27T06:00:00	0	20	4	10		4.04		0.60
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-08-27T06:00:00	0	22	1	10		4.02		0.64
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-08-27T06:00:00	0	22	2	10		4.04		0.63
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-08-27T06:00:00	0	22	3	10		4.09		0.67
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-08-27T06:00:00	0	22	4	10		3.95		0.59
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-08-27T06:00:00	0	24	1	10		4.04		0.64
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-08-27T06:00:00	0	24	2	10		4.01		0.61
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-08-27T06:00:00	0	24	3	10		4.09		0.67
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-08-27T06:00:00	0	24	4	10		3.98		0.61
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-09-10T06:00:00	14	18	1	5	50.00	3.68	90.78	0.67	103.45	56.40	160.84	3.23	0.66	0.57	195.36	217.24	1.11	4.47	0.02	0.30
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-09-10T06:00:00	14	18	2	8	80.00	3.86	94.37	0.66	103.82	77.10	269.52	2.25	0.92	0.70	171.76	346.63	2.02	3.87	0.02	0.39
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-09-10T06:00:00	14	18	3	4	40.00	3.85	94.06	0.69	107.20	102.92	335.99	4.30	0.95	0.70	301.39	438.91	1.46	5.95	0.02	0.39
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-09-10T06:00:00	14	18	4	7	70.00	4.40	109.09	0.69	108.84	65.36	211.27	3.48	0.81	0.49	210.90	276.63	1.31	4.77	0.02	0.33
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-09-10T06:00:00	14	20	1	4	40.00			0.64	101.23	220.11	757.25	41.44	4.46	2.52	1948.50	977.37	0.50	48.43	0.02	0.12
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-09-10T06:00:00	14	20	2	5	50.00	3.92	97.24	0.68	102.09	99.81	364.76	4.56	0.71	0.52	259.11	464.58	1.79	5.79	0.02	0.30
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-09-10T06:00:00	14	20	3	5	50.00	4.12	102.48	0.69	103.79	198.73	710.72	41.69	1.98	1.16	1795.56	909.46	0.51	44.82	0.02	0.06
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-09-10T06:00:00	14	20	4	2	20.00	4.15	102.58	0.67	111.67	200.28	657.89	38.46	5.77	7.76	1877.54	858.16	0.46	51.99	0.03	0.24
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-09-10T06:00:00	14	22	1	4	40.00	3.42	85.10	0.54	83.31	189.25	660.49	34.29	8.34	8.69	1478.49	849.75	0.57	51.32	0.03	0.28
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-09-10T06:00:00	14	22	2	4	40.00	3.58	88.62	0.52	82.07	158.32	580.35	28.84	8.80	5.95	1322.82	738.68	0.56	43.58	0.03	0.28
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-09-10T06:00:00	14	22	3	5	50.00	3.79	92.80	0.60	90.49	53.33	195.04	3.83	1.75	3.19	198.89	248.37	1.25	8.77	0.04	0.52
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-09-10T06:00:00	14	22	4	2	20.00	3.87	97.85	0.47	80.04	155.57	616.20	30.53	18.94	39.29	1093.82	771.77	0.71	88.76	0.08	0.62
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-09-10T06:00:00	14	24	1	1	10.00	2.25	55.79	0.45	71.37	154.41	612.37	28.56	8.93	24.71	1421.28	766.78	0.54	62.21	0.04	0.54
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-09-10T06:00:00	14	24	2	3	30.00	4.00	99.79	0.38	62.11	219.88	799.05	14.46	17.33	78.80	1634.93	1018.93	0.62	110.59	0.07	0.82
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-09-10T06:00:00	14	24	3	1	10.00	3.89	95.08	0.53	78.83	175.86	698.58	20.27	11.29	31.52	1115.29	874.44	0.78	63.07	0.06	0.65
Helgoland_Slatissima_2018b	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2018-09-10T06:00:00	14	24	4	0	0.00	0.34	8.64			129.17	534.89	10.30	8.97	10.82	1212.38	664.05	0.55	30.09	0.02	0.51
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-08-26T06:00:00	0	18	1	7		3.42		0.69		143.22	394.23	7.76	0.74	0.55	329.86	537.44	1.63	9.04	0.03	0.08
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-08-26T06:00:00	0	18	2	7		3.28		0.71		108.87	262.63	3.90	1.67	2.69	268.20	371.50	1.39	8.26	0.03	0.45
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-08-26T06:00:00	0	18	3	7		3.59		0.66		98.28	212.22	1.88	1.29	1.82	190.01	310.50	1.63	4.99	0.03	0.50
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-08-26T06:00:00	0	18	4	7		3.25		0.71		108.29	238.98	3.20	1.82	2.33	208.61	347.27	1.66	7.35	0.04	0.44
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-08-26T06:00:00	0	20	1	7		3.55		0.69
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-08-26T06:00:00	0	20	2	7		2.99		0.70
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-08-26T06:00:00	0	20	3	7		3.41		0.65
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-08-26T06:00:00	0	20	4	7		3.45		0.70
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-08-26T06:00:00	0	22	1	7		3.48		0.63
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-08-26T06:00:00	0	22	2	7		3.31		0.67
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-08-26T06:00:00	0	22	3	7		3.37		0.67
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-08-26T06:00:00	0	22	4	7		3.27		0.56
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-08-26T06:00:00	0	24	1	7		3.26		0.57
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-08-26T06:00:00	0	24	2	7		3.24
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-08-26T06:00:00	0	24	3	7		3.28		0.70
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-26T06:00:00	0	24	4	7		3.28		0.69
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-09T06:00:00	14	18	1	7	100.00	2.96	86.58	0.72	104.73	97.01	313.29	3.56	0.54	3.37	520.47	410.29	0.79	7.47	0.01	0.44
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-09T06:00:00	14	18	2	7	100.00	3.03	92.62	0.74	104.83	132.73	427.74	6.17	1.06	0.00	721.18	560.48	0.78	7.23	0.01	0.07
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-09T06:00:00	14	18	3	4	57.14	1.92	53.38	0.71	108.00	118.98	415.81	5.27	0.00	0.00	658.97	534.79	0.81	5.27	0.01	0.00
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-09T06:00:00	14	18	4	7	100.00	3.02	92.88	0.68	96.91	98.08	316.91	3.80	0.37	0.23	437.22	414.99	0.95	4.39	0.01	0.10
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-09T06:00:00	14	20	1	4	57.14	1.07	30.22	0.68	99.18	173.92	572.73	9.22	0.59	1.12	952.84	746.64	0.78	10.92	0.01	0.17
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-09T06:00:00	14	20	2	7	100.00	2.84	94.91	0.64	92.16	90.47	313.97	5.39	0.53	0.12	358.87	404.44	1.13	6.04	0.02	0.10
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-09T06:00:00	14	20	3	6	85.71	1.86	54.68	0.63	96.96	144.02	462.32	16.48	0.28	0.28	1007.79	606.34	0.60	17.04	0.02	0.07
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-09T06:00:00	14	20	4	5	71.43	1.55	45.01	0.64	91.49	158.31	576.25	8.11	1.31	10.01	1056.96	734.56	0.69	19.43	0.02	0.56
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-09T06:00:00	14	22	1	3	42.86	0.78	22.53	0.59	93.64	149.10	563.90	8.97	1.12	2.24	1059.42	713.00	0.67	12.33	0.01	0.23
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-09T06:00:00	14	22	2	0	0.00
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-09T06:00:00	14	22	3	6	85.71	1.38	40.77	0.51	76.06	114.79	388.72	3.97	0.95	8.27	615.64	503.51	0.82	13.19	0.02	0.44
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-09T06:00:00	14	22	4	7	100.00	1.95	59.61	0.61	108.98	93.64	305.10	3.64	0.27	1.83	552.62	398.74	0.72	5.74	0.01	0.31
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-09T06:00:00	14	24	1	2	28.57	1.74	53.31			155.86	552.20	7.07	4.21	15.57	1171.91	708.06	0.60	26.86	0.02	0.66
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-09T06:00:00	14	24	2	0	0.00
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-09T06:00:00	14	24	3	4	57.14	1.55	47.36	0.40	56.73	151.86	546.09	8.23	1.55	13.59	1105.74	697.95	0.63	23.37	0.02	0.60
Helgoland_Slatissima_2019	laboratory	Phaeophyeae	Saccharina latissima	marinespecies.org	urn:lsid:marinespecies.org:taxname:234482	2019-09-09T06:00:00	14	24	4	3	42.86	1.26	38.44	0.41	59.58	166.83	561.41	7.25	2.18	15.96	1402.80	728.24	0.52	25.39	0.02	0.67