Citation:

PANGAEA.949588

Name: Water record of a two factor (pH and infection) orthogonal experiment in wild Hong Kong oyster population
Creator: Xin Dang
Published: 2022-10-11
License: https://creativecommons.org/licenses/by/4.0/
Keywords: experiment; foodborne disease control; Hong Kong oyster; immune tolerance; Local adaptation; pathogen; wild population

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook TwitterShow Map Google Earth

Abstract:

The basic observed water characteristics (pH, temperature, salinity and total alkalinity (TA)) and calculated water carbonate chemistry in the field (22.44°N, 113.95°E, Pak Nai, Hong Kong) and laboratory (22.28°N, 114.14°E, the University of Hong Kong, Hong Kong) during a two-factor orthogonal experiment. To be specific, we designed a two-factor (low pH, as a proxy for OA, and Vibrio infection) orthogonal experiment to examine the immunological and molecular responses in the ecologically and economically important edible Hong Kong oyster, Crassostrea hongkongensis. Wild oyster population was collected from their native coastal area (22.44°N, 113.95°E, Pak Nai, Hong Kong). Vibrio parahaemolyticus, as a major global concern of foodborne human-related disease, was used in this study to investigate the antimicrobial characteristics of edible commercial oysters in the context of future OA. pH and temperature were monitored daily with pH meter (Mettler Toledo InLab®, Switzerland) calibrated with NBS standards. Salinity was monitored using a dual-scale refractometer (MingRui, China). The seawater was utterly changed every two days and 50 mL seawater was preserved with HgCl2 every week for total alkalinity (TA) measurement. The TA was measured using an alkalinity titrator (Mettler Toledo G20S Compact Titrator, Switzerland), and pCO2, CO32-, saturation states of calcite and aragonite were calculated by CO2SYS program with equilibrium constants K2 and KSO4 (Millero, F. J., Graham, T. B., Huang, F., Bustos-Serrano, H., & Pierrot, D. (2006). Dissociation constants of carbonic acid in seawater as a function of salinity and temperature. Marine Chemistry, 100(1), 80-94. https://doi.org/10.1016/j.marchem.2005.12.001).

Keyword(s):

experiment; foodborne disease control; Hong Kong oyster; immune tolerance; Local adaptation; pathogen; wild population

Supplement to:

Dang, Xin; Lee, Tin Hang; Thiyagarajan, Vengatesen (2023): Wild oyster population resistance to ocean acidification adversely affected by bacterial infection. Environmental Pollution, 317, 120813, https://doi.org/10.1016/j.envpol.2022.120813

Coverage:

Median Latitude: 22.360000 * Median Longitude: 114.045000 * South-bound Latitude: 22.280000 * West-bound Longitude: 113.950000 * North-bound Latitude: 22.440000 * East-bound Longitude: 114.140000

Date/Time Start: 2021-03-25T00:00:00 * Date/Time End: 2021-05-10T00:00:00

Event(s):

Pak_Nai_Experiment * Latitude: 22.280000 * Longitude: 114.140000 * Date/Time Start: 2021-04-09T00:00:00 * Date/Time End: 2021-05-10T00:00:00 * Location: Hong Kong, Region of the People's Republic of China * Method/Device: Laboratory experiment

Pak_Nai_Field * Latitude: 22.440000 * Longitude: 113.950000 * Date/Time: 2021-03-25T00:00:00 * Location: Hong Kong, Region of the People's Republic of China * Method/Device: Hand picking

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Dang, Xin
2	DATE/TIME	Date/Time		Dang, Xin		Geocode
3	Experimental treatment	Exp treat		Dang, Xin
4	pH	pH		Dang, Xin	pH meter (Mettler Toledo InLab Semi-Micro)
5	Temperature, water	Temp	°C	Dang, Xin	pH meter (Mettler Toledo InLab Semi-Micro)
6	Salinity	Sal		Dang, Xin	Dual-scale refractometer (MingRui, China)
7	Alkalinity, total	AT	µmol/kg	Dang, Xin	Alkalinity titrator (Mettler Toledo G20S Compact Titrator, Switzerland)
8	Salinity	Sal		Dang, Xin	Dual-scale refractometer (MingRui, China)
9	Temperature, water	Temp	°C	Dang, Xin	pH meter (Mettler Toledo InLab Semi-Micro)
10	pH	pH		Dang, Xin	pH meter (Mettler Toledo InLab Semi-Micro)
11	Carbon dioxide, partial pressure	pCO2	µatm	Dang, Xin	Calculated using CO2SYS
12	Carbonate ion	[CO3]2-	µmol/kg	Dang, Xin	Calculated using CO2SYS
13	Calcite saturation state	Omega Cal		Dang, Xin	Calculated using CO2SYS
14	Aragonite saturation state	Omega Arg		Dang, Xin	Calculated using CO2SYS

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC) * Processing Level: PANGAEA data processing level 4 (ProcLevel4)

Size:

921 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 Date/Time	3 Exp treat	4 pH (pH meter (Mettler Toledo InLa...)	5 Temp [°C] (pH meter (Mettler Toledo InLa...)	6 Sal (Dual-scale refractometer (Min...)	7 AT [µmol/kg] (Alkalinity titrator (Mettler ...)	8 Sal (Dual-scale refractometer (Min...)	9 Temp [°C] (pH meter (Mettler Toledo InLa...)	10 pH (pH meter (Mettler Toledo InLa...)	11 pCO2 [µatm] (Calculated using CO2SYS)	12 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	13 Omega Cal (Calculated using CO2SYS)	14 Omega Arg (Calculated using CO2SYS)
Pak_Nai_Field	2021-03-25	Field				1417	30.4	22.5	7.70	893.8980	41.83900	1.038945991	0.672523433
Pak_Nai_Experiment	2021-04-09	OA1	7.38	22.3	25
Pak_Nai_Experiment	2021-04-09	OA2	7.29	22.4	25
Pak_Nai_Experiment	2021-04-09	OA3	7.31	22.8	25
Pak_Nai_Experiment	2021-04-09	Control1	7.79	21.5	25
Pak_Nai_Experiment	2021-04-09	Control2	7.86	21.3	25
Pak_Nai_Experiment	2021-04-09	Control3	7.81	21.2	25
Pak_Nai_Experiment	2021-04-10	OA1	7.36	21.9	25
Pak_Nai_Experiment	2021-04-10	OA2	7.30	22.1	25
Pak_Nai_Experiment	2021-04-10	OA3	7.32	22.3	25
Pak_Nai_Experiment	2021-04-10	Control1	8.00	21.7	25
Pak_Nai_Experiment	2021-04-10	Control2	7.99	22.1	25
Pak_Nai_Experiment	2021-04-10	Control3	8.01	22.7	25
Pak_Nai_Experiment	2021-04-11	OA1	7.30	21.9	25
Pak_Nai_Experiment	2021-04-11	OA2	7.30	22.0	25
Pak_Nai_Experiment	2021-04-11	OA3	7.29	22.9	25
Pak_Nai_Experiment	2021-04-11	Control1	7.83	21.6	25
Pak_Nai_Experiment	2021-04-11	Control2	7.86	21.5	25
Pak_Nai_Experiment	2021-04-11	Control3	7.78	22.6	25
Pak_Nai_Experiment	2021-04-12	OA1	7.32	22.1	25
Pak_Nai_Experiment	2021-04-12	OA2	7.21	22.1	25
Pak_Nai_Experiment	2021-04-12	OA3	7.20	22.6	25
Pak_Nai_Experiment	2021-04-12	Control1	7.85	21.8	25
Pak_Nai_Experiment	2021-04-12	Control2	7.82	22.1	25
Pak_Nai_Experiment	2021-04-12	Control3	7.81	22.3	25
Pak_Nai_Experiment	2021-04-13	OA1	7.49	22.0	25
Pak_Nai_Experiment	2021-04-13	OA2	7.27	22.1	25
Pak_Nai_Experiment	2021-04-13	OA3	7.29	22.1	25
Pak_Nai_Experiment	2021-04-13	Control1	7.77	22.1	25
Pak_Nai_Experiment	2021-04-13	Control2	7.84	22.4	25
Pak_Nai_Experiment	2021-04-13	Control3	7.82	23.1	25
Pak_Nai_Experiment	2021-04-14	OA1	7.22	22.3	25
Pak_Nai_Experiment	2021-04-14	OA2	7.26	22.3	25
Pak_Nai_Experiment	2021-04-14	OA3	7.28	22.9	25
Pak_Nai_Experiment	2021-04-14	Control1	7.89	21.9	25
Pak_Nai_Experiment	2021-04-14	Control2	7.88	22.5	25
Pak_Nai_Experiment	2021-04-14	Control3	7.86	22.6	25
Pak_Nai_Experiment	2021-04-15	OA1	7.31	22.5	26
Pak_Nai_Experiment	2021-04-15	OA2	7.33	22.5	25
Pak_Nai_Experiment	2021-04-15	OA3	7.35	23.2	27
Pak_Nai_Experiment	2021-04-15	Control1	7.86	22.1	26
Pak_Nai_Experiment	2021-04-15	Control2	7.87	22.4	26
Pak_Nai_Experiment	2021-04-15	Control3	7.87	22.9	26
Pak_Nai_Experiment	2021-04-16	OA1	7.13	22.8	28	2184	28.0	22.8	7.13	5556.7573	17.59581	0.444879878	0.285830375
Pak_Nai_Experiment	2021-04-16	OA2	7.18	22.7	28	2160	28.0	22.7	7.18	4881.5928	19.41769	0.490821131	0.315261513
Pak_Nai_Experiment	2021-04-16	OA3	7.12	22.9	30	2072	30.0	22.9	7.12	5323.0972	17.17216	0.428023685	0.277021453
Pak_Nai_Experiment	2021-04-16	Control1	7.88	22.5	27	1700	27.0	22.5	7.88	707.9199	68.21771	1.735813145	1.109910978
Pak_Nai_Experiment	2021-04-16	Control2	7.85	22.8	30	1687	30.0	22.8	7.85	737.1771	68.43747	1.705467409	1.103492044
Pak_Nai_Experiment	2021-04-16	Control3	7.84	23.4	27	1669	27.0	23.4	7.84	773.6220	63.24712	1.613231946	1.034050015
Pak_Nai_Experiment	2021-04-17	OA1	7.18	22.5	25
Pak_Nai_Experiment	2021-04-17	OA2	7.19	22.4	25
Pak_Nai_Experiment	2021-04-17	OA3	7.20	22.7	25
Pak_Nai_Experiment	2021-04-17	Control1	7.90	22.0	25
Pak_Nai_Experiment	2021-04-17	Control2	7.92	22.3	25
Pak_Nai_Experiment	2021-04-17	Control3	7.94	22.9	25
Pak_Nai_Experiment	2021-04-18	OA1	7.13	22.3	26
Pak_Nai_Experiment	2021-04-18	OA2	7.16	22.2	26
Pak_Nai_Experiment	2021-04-18	OA3	7.15	22.5	26
Pak_Nai_Experiment	2021-04-18	Control1	7.88	22.4	26
Pak_Nai_Experiment	2021-04-18	Control2	7.95	22.1	26
Pak_Nai_Experiment	2021-04-18	Control3	7.95	22.5	25
Pak_Nai_Experiment	2021-04-19	OA1	7.17	22.1	27
Pak_Nai_Experiment	2021-04-19	OA2	7.10	22.0	27
Pak_Nai_Experiment	2021-04-19	OA3	7.17	22.3	28
Pak_Nai_Experiment	2021-04-19	Control1	7.75	21.9	27
Pak_Nai_Experiment	2021-04-19	Control2	7.85	22.1	27
Pak_Nai_Experiment	2021-04-19	Control3	7.87	22.3	27
Pak_Nai_Experiment	2021-04-20	OA1	7.25	22.0	25
Pak_Nai_Experiment	2021-04-20	OA2	7.26	21.9	25
Pak_Nai_Experiment	2021-04-20	OA3	7.22	22.1	25
Pak_Nai_Experiment	2021-04-20	Control1	7.86	21.6	25
Pak_Nai_Experiment	2021-04-20	Control2	7.89	21.9	25
Pak_Nai_Experiment	2021-04-20	Control3	7.91	22.3	25
Pak_Nai_Experiment	2021-04-21	OA1	7.37	22.0	26
Pak_Nai_Experiment	2021-04-21	OA2	7.41	22.0	26
Pak_Nai_Experiment	2021-04-21	OA3	7.43	22.4	25
Pak_Nai_Experiment	2021-04-21	Control1	7.88	21.6	27
Pak_Nai_Experiment	2021-04-21	Control2	7.89	21.9	25
Pak_Nai_Experiment	2021-04-21	Control3	7.89	22.3	26
Pak_Nai_Experiment	2021-04-22	OA1	7.42	22.4	25
Pak_Nai_Experiment	2021-04-22	OA2	7.36	22.4	25
Pak_Nai_Experiment	2021-04-22	OA3	7.39	23.1	25
Pak_Nai_Experiment	2021-04-22	Control1	7.97	22.3	25
Pak_Nai_Experiment	2021-04-22	Control2	7.98	22.7	25
Pak_Nai_Experiment	2021-04-22	Control3	7.96	22.9	25
Pak_Nai_Experiment	2021-04-23	OA1	7.41	22.5	26
Pak_Nai_Experiment	2021-04-23	OA2	7.33	22.7	25
Pak_Nai_Experiment	2021-04-23	OA3	7.38	23.4	26
Pak_Nai_Experiment	2021-04-23	Control1	7.86	22.4	26
Pak_Nai_Experiment	2021-04-23	Control2	7.85	22.8	25
Pak_Nai_Experiment	2021-04-23	Control3	7.88	23.1	25
Pak_Nai_Experiment	2021-04-24	OA1	7.34	22.1	25	2015	25.0	22.1	7.34	3180.0952	23.66367	0.610047381	0.386232354
Pak_Nai_Experiment	2021-04-24	OA2	7.34	22.2	25	2002	25.0	22.2	7.34	3162.4148	23.58457	0.608186188	0.385154771
Pak_Nai_Experiment	2021-04-24	OA3	7.32	22.9	26	2022	26.0	22.9	7.32	3342.0151	23.86223	0.612207695	0.390202866
Pak_Nai_Experiment	2021-04-24	Control1	7.95	21.9	25	1866	25.0	21.9	7.95	663.1146	81.52562	2.100502155	1.329173970
Pak_Nai_Experiment	2021-04-24	Control2	7.92	22.3	25	1857	25.0	22.3	7.92	714.1230	77.15224	1.990146053	1.260658982
Pak_Nai_Experiment	2021-04-24	Control3	7.87	22.6	27	1862	27.0	22.6	7.87	797.2859	73.53328	1.871560550	1.197031383
Pak_Nai_Experiment	2021-04-25	OA1	7.39	21.6	27
Pak_Nai_Experiment	2021-04-25	OA2	7.40	21.6	27
Pak_Nai_Experiment	2021-04-25	OA3	7.35	22.2	27
Pak_Nai_Experiment	2021-04-25	Control1	7.99	21.3	27
Pak_Nai_Experiment	2021-04-25	Control2	7.97	21.7	27
Pak_Nai_Experiment	2021-04-25	Control3	7.96	22.1	28
Pak_Nai_Experiment	2021-04-26	OA1	7.33	21.3	27
Pak_Nai_Experiment	2021-04-26	OA2	7.27	21.4	27
Pak_Nai_Experiment	2021-04-26	OA3	7.36	21.8	27
Pak_Nai_Experiment	2021-04-26	Control1	7.98	21.0	27
Pak_Nai_Experiment	2021-04-26	Control2	7.97	21.4	27
Pak_Nai_Experiment	2021-04-26	Control3	7.94	22.0	27
Pak_Nai_Experiment	2021-04-27	OA1	7.35	21.1	27
Pak_Nai_Experiment	2021-04-27	OA2	7.35	21.3	26
Pak_Nai_Experiment	2021-04-27	OA3	7.37	21.7	26
Pak_Nai_Experiment	2021-04-27	Control1	7.96	21.0	27
Pak_Nai_Experiment	2021-04-27	Control2	7.94	21.3	26
Pak_Nai_Experiment	2021-04-27	Control3	7.93	21.8	27
Pak_Nai_Experiment	2021-04-28	OA1	7.34	21.1	26
Pak_Nai_Experiment	2021-04-28	OA2	7.34	21.1	25
Pak_Nai_Experiment	2021-04-28	OA3	7.37	21.6	27
Pak_Nai_Experiment	2021-04-28	Control1	7.96	21.0	27
Pak_Nai_Experiment	2021-04-28	Control2	7.93	21.2	27
Pak_Nai_Experiment	2021-04-28	Control3	7.92	22.4	27
Pak_Nai_Experiment	2021-04-29	OA1	7.36	21.0	27
Pak_Nai_Experiment	2021-04-29	OA2	7.45	20.9	26
Pak_Nai_Experiment	2021-04-29	OA3	7.34	21.3	26
Pak_Nai_Experiment	2021-04-29	Control1	7.93	20.8	27
Pak_Nai_Experiment	2021-04-29	Control2	7.90	21.0	27
Pak_Nai_Experiment	2021-04-29	Control3	7.97	21.5	26
Pak_Nai_Experiment	2021-04-30	OA1	7.29	21.3	26	1965	26.0	21.3	7.29	3434.3999	20.61254	0.526520891	0.334178659
Pak_Nai_Experiment	2021-04-30	OA2	7.29	21.3	26	2012	26.0	21.3	7.29	3516.9229	21.10782	0.539172347	0.342208438
Pak_Nai_Experiment	2021-04-30	OA3	7.32	22.2	26	2082	26.0	22.2	7.32	3419.4124	24.03940	0.615542368	0.391597523
Pak_Nai_Experiment	2021-04-30	Control1	8.00	20.8	26	1898	26.0	20.8	8.00	582.6859	90.96377	2.320555266	1.470956465
Pak_Nai_Experiment	2021-04-30	Control2	8.01	21.1	26	1933	26.0	21.1	8.01	579.7009	95.39992	2.435599862	1.545062433
Pak_Nai_Experiment	2021-04-30	Control3	7.93	21.6	26	1865	26.0	21.6	7.93	688.2947	79.24221	2.025745549	1.286722350
Pak_Nai_Experiment	2021-05-01	OA1	7.29	21.3	27
Pak_Nai_Experiment	2021-05-01	OA2	7.29	21.4	27
Pak_Nai_Experiment	2021-05-01	OA3	7.29	22.8	27
Pak_Nai_Experiment	2021-05-01	Control1	8.00	21.1	27
Pak_Nai_Experiment	2021-05-01	Control2	7.99	21.4	28
Pak_Nai_Experiment	2021-05-01	Control3	7.96	21.7	29
Pak_Nai_Experiment	2021-05-02	OA1	7.25	21.4	27
Pak_Nai_Experiment	2021-05-02	OA2	7.27	21.2	27
Pak_Nai_Experiment	2021-05-02	OA3	7.27	21.5	27
Pak_Nai_Experiment	2021-05-02	Control1	7.97	21.3	27
Pak_Nai_Experiment	2021-05-02	Control2	7.97	21.8	27
Pak_Nai_Experiment	2021-05-02	Control3	7.99	22.6	27
Pak_Nai_Experiment	2021-05-03	OA1	7.15	21.6	27
Pak_Nai_Experiment	2021-05-03	OA2	7.25	21.4	27
Pak_Nai_Experiment	2021-05-03	OA3	7.28	21.8	27
Pak_Nai_Experiment	2021-05-03	Control1	7.95	21.4	27
Pak_Nai_Experiment	2021-05-03	Control2	7.88	21.5	27
Pak_Nai_Experiment	2021-05-03	Control3	7.84	22.0	27
Pak_Nai_Experiment	2021-05-04	OA1	7.26	21.9	26
Pak_Nai_Experiment	2021-05-04	OA2	7.29	21.9	26
Pak_Nai_Experiment	2021-05-04	OA3	7.29	22.4	26
Pak_Nai_Experiment	2021-05-04	Control1	8.00	21.9	26
Pak_Nai_Experiment	2021-05-04	Control2	7.98	22.3	26
Pak_Nai_Experiment	2021-05-04	Control3	8.00	22.5	26
Pak_Nai_Experiment	2021-05-05	OA1	7.35	21.9	27
Pak_Nai_Experiment	2021-05-05	OA2	7.32	21.8	27
Pak_Nai_Experiment	2021-05-05	OA3	7.29	21.9	27
Pak_Nai_Experiment	2021-05-05	Control1	7.97	21.8	27
Pak_Nai_Experiment	2021-05-05	Control2	7.94	21.7	27
Pak_Nai_Experiment	2021-05-05	Control3	7.93	21.8	27
Pak_Nai_Experiment	2021-05-06	OA1	7.25	21.5	25
Pak_Nai_Experiment	2021-05-06	OA2	7.26	21.4	25
Pak_Nai_Experiment	2021-05-06	OA3	7.19	21.6	25
Pak_Nai_Experiment	2021-05-06	Control1	7.99	21.2	25
Pak_Nai_Experiment	2021-05-06	Control2	7.95	21.4	25
Pak_Nai_Experiment	2021-05-06	Control3	7.99	21.5	25
Pak_Nai_Experiment	2021-05-07	OA1	7.21	21.6	25
Pak_Nai_Experiment	2021-05-07	OA2	7.27	21.6	25
Pak_Nai_Experiment	2021-05-07	OA3	7.29	21.7	25
Pak_Nai_Experiment	2021-05-07	Control1	7.97	21.6	25
Pak_Nai_Experiment	2021-05-07	Control2	7.93	21.5	25
Pak_Nai_Experiment	2021-05-07	Control3	7.98	21.3	25
Pak_Nai_Experiment	2021-05-08	OA1	7.18	21.5	25
Pak_Nai_Experiment	2021-05-08	OA2	7.33	21.2	25
Pak_Nai_Experiment	2021-05-08	OA3	7.32	21.2	25
Pak_Nai_Experiment	2021-05-08	Control1	7.89	21.2	25
Pak_Nai_Experiment	2021-05-08	Control2	7.93	21.0	25
Pak_Nai_Experiment	2021-05-08	Control3	7.98	21.0	25
Pak_Nai_Experiment	2021-05-09	OA1	7.27	21.8	25
Pak_Nai_Experiment	2021-05-09	OA2	7.26	21.6	25
Pak_Nai_Experiment	2021-05-09	OA3	7.26	21.9	25
Pak_Nai_Experiment	2021-05-09	Control1	7.95	21.4	25
Pak_Nai_Experiment	2021-05-09	Control2	7.92	21.6	25
Pak_Nai_Experiment	2021-05-09	Control3	7.99	21.6	25
Pak_Nai_Experiment	2021-05-10	OA1	7.29	22.5	25
Pak_Nai_Experiment	2021-05-10	OA2	7.31	22.4	25
Pak_Nai_Experiment	2021-05-10	OA3	7.27	22.2	25
Pak_Nai_Experiment	2021-05-10	Control1	8.02	22.3	25
Pak_Nai_Experiment	2021-05-10	Control2	7.98	22.3	25
Pak_Nai_Experiment	2021-05-10	Control3	8.05	22.2	25