Citation:

PANGAEA.949506

Name: Molecular basis of ocean acidification sensitivity and adaptation in Mytilus galloprovincialis
Published: 2022-10-10
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gene expression (incl. proteomics); Laboratory experiment; Mediterranean Sea; Mollusca; Mytilus galloprovincialis; Pelagos; Single species; Temperate; Zooplankton

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Abstract:

Predicting the potential for species adaption to climate change is challenged by the need to identify the physiological mechanisms that underpin species vulnerability. Here we investigated the sensitivity to ocean acidification in marine mussels during early development, and specifically the trochophore stage. Using RNA and DNA sequencing and in situ RNA hybridization, we identified developmental processes associated with abnormal development and rapid adaptation to low pH. Trochophores exposed to low pH seawater exhibited 43 differentially expressed genes. Gene annotation and in situ hybridization of differentially expressed genes point to pH sensitivity of (1) shell field development and (2) cellular stress response. Five genes within these two processes exhibited shifts in allele frequencies indicative of a potential for rapid adaptation. This case study contributes direct evidence that protecting species' existing genetic diversity is a critical management action to facilitate species resilience to climate change.

Keyword(s):

Animalia; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gene expression (incl. proteomics); Laboratory experiment; Mediterranean Sea; Mollusca; Mytilus galloprovincialis; Pelagos; Single species; Temperate; Zooplankton

Supplement to:

Kapsenberg, Lydia; Bitter, M C; Miglioli, A; Aparicio-Estalella, Clàudia; Pelejero, Carles; Gattuso, Jean-Pierre; Dumollard, R (2022): Molecular basis of ocean acidification sensitivity and adaptation in Mytilus galloprovincialis. iScience, 25(8), 104677, https://doi.org/10.1016/j.isci.2022.104677

Related to:

Kapsenberg, Lydia; Miglioli, A; Bitter, M C; Tambutté, Eric; Dumollard, R; Gattuso, Jean-Pierre (2019): Developmental morphology data of mussel larvae grown in static and fluctuating pH treatments. PANGAEA, https://doi.org/10.1594/PANGAEA.899830

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 43.682000 * Longitude: 7.319000

Date/Time Start: 2017-02-09T00:00:00 * Date/Time End: 2017-02-09T00:00:00

Event(s):

Villefranche_sur_Mer * Latitude: 43.682000 * Longitude: 7.319000 * Date/Time: 2017-02-09T00:00:00 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-10-10.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Kapsenberg, Lydia		Study
2	Species, unique identification	Species UID		Kapsenberg, Lydia
3	Species, unique identification (URI)	Species UID (URI)		Kapsenberg, Lydia
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Kapsenberg, Lydia
5	Accession number	Access No		Kapsenberg, Lydia
6	Gene name	Gene		Kapsenberg, Lydia
7	Experiment	Exp		Kapsenberg, Lydia
8	Sample ID	Sample ID		Kapsenberg, Lydia		qPCR
9	Replicates	Repl	#	Kapsenberg, Lydia
10	Treatment	Treat		Kapsenberg, Lydia
11	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Kapsenberg, Lydia
12	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Kapsenberg, Lydia		Ref
13	Δ threshold cycle, quantitative polymerase chain reaction	Δ Ct qPCR		Kapsenberg, Lydia
14	Δ threshold cycle, quantitative polymerase chain reaction	Δ Ct qPCR		Kapsenberg, Lydia		Amb_Ave
15	Δ threshold cycle, quantitative polymerase chain reaction	Δ Ct qPCR		Kapsenberg, Lydia		ddCT
16	Fold change	Fold change		Kapsenberg, Lydia		log2
17	Time in days	Time	days	Kapsenberg, Lydia
18	Frequency	Frequency		Kapsenberg, Lydia		allele
19	pH	pH		Kapsenberg, Lydia		total scale, min
20	Aragonite saturation state	Omega Arg		Kapsenberg, Lydia		min
21	Ratio	Ratio		Kapsenberg, Lydia		[HCO3-]/[H+](mol/?mol, min)
22	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Kapsenberg, Lydia		min
23	pH	pH		Kapsenberg, Lydia		total scale, max
24	Aragonite saturation state	Omega Arg		Kapsenberg, Lydia		max
25	Ratio	Ratio		Kapsenberg, Lydia		[HCO3-]/[H+](mol/?mol, max)
26	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Kapsenberg, Lydia		max
27	Temperature, water	Temp	°C	Kapsenberg, Lydia
28	Temperature, water, standard deviation	Temp std dev	±	Kapsenberg, Lydia
29	Salinity	Sal		Kapsenberg, Lydia
30	Salinity, standard deviation	Sal std dev	±	Kapsenberg, Lydia
31	Alkalinity, total	AT	µmol/kg	Kapsenberg, Lydia
32	Alkalinity, total, standard deviation	AT std dev	±	Kapsenberg, Lydia
33	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	max
35	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	max
36	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	max
37	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	max
38	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	min
39	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	max
40	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	min
41	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	min
42	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	min
43	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	min
44	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	min
45	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	min
46	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	max
47	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	min
48	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	max
49	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	max

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

10635 data points

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