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Dahlke, Flemming; Puvanendran, Velmurugu; Mortensen, Atle; Pörtner, Hans-Otto; Storch, Daniela (2022): Seawater carbonate chemistry and gamete quality and window of fertilisation in Atlantic cod [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.949249

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Abstract:
Impacts of global warming and CO2-related ocean acidification (OA) on fish reproduction may include chronic effects on gametogenesis and gamete quality, as well as acute effects on external fertilisation. Here, temperature thresholds and OA-sensitivity of gametogenesis and fertilisation were investigated in Atlantic cod, Gadus morhua. Three broodstock groups of farmed cod (FC 1-3) were exposed for three months to three maturation conditions (FC 1: Control, 6°C/400 μatm CO2; FC 2: Warming, 9.5°C/400 μatm; FC 3: Warming & OA, 9.5°C/1100 μatm). In addition, a broodstock group of wild cod (WC) was kept at control conditions to compare the acute temperature window of fertilisation with that of farmed cod (FC 1). Fertilisations were conducted in a temperature-gradient table at ten temperatures (between −1.5 and 12°C) and two CO2 levels (400/1100 μatm). In FC 1 and WC, fertilisation success was relatively high between 0.5°C and 11°C (TRange of ~10.5°C), indicating similar gamete quality in farmed and wild broodstocks kept at control conditions. Exposure of farmed broodstocks to warming (FC 2) and the combination of warming and OA (FC 3) impaired gamete quality, causing a reduction in fertilisation success of −20% (FC 2) and −42% (FC 3) compared to FC 1. The acute temperature window of fertilisation narrowed from FC 1 (TRange = 10.4°C) to FC 2 (TRange = 8.8°C) and FC 3 (TRange = 5.9°C). Acute effects of CO2 on fertilisation success were not significant. This study demonstrates potential climate change impacts on gametogenesis and fertilisation in Atlantic cod, suggesting the loss of spawning habitat in the coming decades.
Keyword(s):
Animalia; Chordata; Coast and continental shelf; Gadus morhua; Laboratory experiment; Mesocosm or benthocosm; Nekton; North Atlantic; Pelagos; Polar; Reproduction; Single species; Temperature
Supplement to:
Dahlke, Flemming; Puvanendran, Velmurugu; Mortensen, Atle; Pörtner, Hans-Otto; Storch, Daniela (2022): Broodstock exposure to warming and elevated p CO 2 impairs gamete quality and narrows the temperature window of fertilisation in Atlantic cod. Journal of Fish Biology, jfb.15140, https://doi.org/10.1111/jfb.15140
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html
Coverage:
Latitude: 70.250000 * Longitude: 19.000000
Date/Time Start: 2014-03-01T00:00:00 * Date/Time End: 2014-03-31T00:00:00
Event(s):
Barents_Sea * Latitude: 70.250000 * Longitude: 19.000000 * Date/Time Start: 2014-03-01T00:00:00 * Date/Time End: 2014-03-31T00:00:00 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-09-30.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeDahlke, FlemmingStudy
2Species, unique identificationSpecies UIDDahlke, Flemming
3Species, unique identification (URI)Species UID (URI)Dahlke, Flemming
4Species, unique identification (Semantic URI)Species UID (Semantic URI)Dahlke, Flemming
5IdentificationIDDahlke, FlemmingBroodstock
6IdentificationIDDahlke, FlemmingFemale
7Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmDahlke, FlemmingFertilisation
8Temperature, waterTemp°CDahlke, FlemmingFertilisation
9Egg stagesEgg%Dahlke, Flemmingregular
10Egg stagesEgg%Dahlke, Flemmingirregular
11Egg stagesEgg%Dahlke, Flemmingnecrotic
12Egg stagesEgg%Dahlke, Flemmingunfertilized
13Egg stagesEgg%Dahlke, Flemmingnonviable
14Temperature, waterTemp°CDahlke, FlemmingBroodstock condition
15pHpHDahlke, FlemmingBroodstock condition, free scale
16Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmDahlke, FlemmingBroodstock condition
17Eggs, diameterEggs diammmDahlke, Flemming
18Alkalinity, totalATµmol/kgDahlke, FlemmingBroodstock condition
19SalinitySalDahlke, FlemmingBroodstock condition
20pHpHDahlke, Flemmingfertilisation experiment, free scale, start
21pHpHDahlke, Flemmingfertilisation experiment, free scale, end
22Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmDahlke, Flemmingfertilisation experiment, start
23Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmDahlke, Flemmingfertilisation experiment, end
24pHpHDahlke, Flemmingfertilisation experiment, free scale, mean
25Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmDahlke, Flemmingfertilisation experiment, mean
26Alkalinity, totalATµmol/kgDahlke, Flemmingfertilisation experiment
27SalinitySalDahlke, Flemmingfertilisation experiment
28Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)Broodstock condition, total scale
30Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)Broodstock condition
31Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)Broodstock condition
32Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)Broodstock condition
33Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)Broodstock condition
34Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)Broodstock condition
35Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)Broodstock condition
36Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)Broodstock condition
37Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)Broodstock condition
38pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)fertilisation experiment, total scale
39Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)fertilisation experiment
40Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)fertilisation experiment
41Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)fertilisation experiment
42Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)fertilisation experiment
43Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)fertilisation experiment
44Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)fertilisation experiment
45Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)fertilisation experiment
46Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)fertilisation experiment
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
17512 data points

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