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Fischer, Jonas; Talal, Ghanem; Schnee, Laura Sophie; Otomo, Patricks; Filser, Juliane (2022): Reproduction test on the springtail Folsomia candida exposed via artificial test soils in a laboratory experiment [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.949200, In: Fischer, J et al. (2022): Physiological, molecular and behavioural effects of copper oxide nanoparticles and copper chloride on the springtail Folsomia candida exposed via artificial test soils in a laboratory experiment [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.949202

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Abstract:
In a laboratory experiment, the soil invertebrate Folsomia candida (Collembola) was exposed to CuCl2 or copper oxide nanoparticles (CuO-NP) for 28 days. The animals were exposed via artificial test soil which varied in the size (19 or 30% of total mass) or the clay type of their clay fraction (kaolin or montmorillonite). The test substances were dissolved (CuCl2) or dispersed (CuO-NP) in pure miliQ water and stirred into the dry test soils to end up with test concentrations of 1, 3, 10 and 32 mg Cu/kg and a water content of 50% of the maximum water holding capacity of the respective soils. As a control, soil with miliQ water only was tested. At the beginning of the experiment, 5 animals at the age of 10-12 days were inserted into the test vessels containing the spiked test soil. After 28 days, the previously inserted adult animals and the juvenile animals produced within the last 28 days were extracted by floatation of the test soils with tap water. The water surface was photographed and the number of produced juveniles was counted manually with the computer programme ImageJ to assess the reproductive output of the animals and their survival rate. The adult animals were collected from the water surface and further analysed for dry mass and Cu body concentration. The endpoints survival, reproduction and dry mass per individual were referred to the average values of the negative control. All endpoints were statistically compared to the control by a linear model.
Keyword(s):
Artificial test soils; Catalase; copper; Copper oxide nanoparticles; Dry weight; Folsomia candida; Kaolin; Metallothionein; Montmorillonite; Moulting; Reproduction; Survival
Event(s):
Laboratory-experiments * Method/Device: Experiment (EXP)
Comment:
Funded by :
Hans‐Böckler‐Stiftung within the framework of the graduate school NanoCompetence (PK041). Open Access funding enabled and organized by Projekt DEAL.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
ExperimentExpFischer, JonasTest name
Soil nameSoil nameFischer, JonasLaboratory experimentTest soil
TreatmentTreatFischer, JonasLaboratory experimentTreatment: test substance (NP, S) and the soil concentration of the respective Cu compound (1, 3, 10 or 32 mg Cu/kg), eg 3 mg Cu/kg with CuO-NP = NP-3; control (C).
Copper, per soil dry massCu soil dmmg/kgFischer, JonasLaboratory experiment
Test substanceTest subsFischer, JonasLaboratory experimentTest substances: CuCl2 (S) and CuO-NP (NP), control (C) only contains miliQ water
ReplicatesRepl#Fischer, JonasLaboratory experiment
Folsomia candida, adults, survivedF. candida ad survived#Fischer, JonasLaboratory experiment
Folsomia candida, juvenileF. candida juv#Fischer, JonasLaboratory experiment
Folsomia candidaF. candida#Fischer, JonasLaboratory experimentF. candida adults used for biomass analytics
10 Folsomia candida, total, dry massF. candida tot dmµgFischer, JonasLaboratory experimentAdults
11 Folsomia candida, dry mass per individualF. candida ind dmµgFischer, JonasLaboratory experiment
12 CopperCungFischer, JonasLaboratory experimentCu content of sample
13 Folsomia candida, survivedF. candida survived%Fischer, JonasLaboratory experiment
14 Folsomia candida, juvenileF. candida juv%Fischer, JonasLaboratory experiment
15 Folsomia candida, dry massF. candida dm%Fischer, JonasLaboratory experiment
16 Sample commentSample commentFischer, JonasLaboratory experimentData censoring criteria for Cu content
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
3243 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Exp

Soil name

Treat

Cu soil dm [mg/kg]

Test subs

Repl [#]

F. candida ad survived [#]

F. candida juv [#]

F. candida [#]
10 
F. candida tot dm [µg]
11 
F. candida ind dm [µg]
12 
Cu [ng]
13 
F. candida survived [%]
14 
F. candida juv [%]
15 
F. candida dm [%]
16 
Sample comment
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