Cohen‐Rengifo, Mishal; Cabon, Joëlle; Danion, Morgane; Mazurais, David (2022): Seawater carbonate chemistry and survival rate of F2 Dicentrarchus labrax after viral challenge [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.949153
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Abstract:
Background: Progressive CO2-induced ocean acidification (OA) impacts marine life in ways that are difficult to predict but are likely to become exacerbated over generations. Although marine fishes can balance acid–base homeostasis efficiently, indirect ionic regulation that alter neurosensory systems can result in behavioural abnormalities. In marine invertebrates, OA can also affect immune system function, but whether this is the case in marine fishes is not fully understood. Farmed fish are highly susceptible to disease outbreak, yet strategies for overcoming such threats in the wake of OA are wanting. Here, we exposed two generations of the European sea bass (Dicentrarchus labrax) to end-of-century predicted pH levels (IPCC RCP8.5), with parents (F1) being exposed for four years and their offspring (F2) for 18 months. Our design included a transcriptomic analysis of the olfactory rosette (collected from the F2) and a viral challenge (exposing F2 to betanodavirus) where we assessed survival rates.
Results: We discovered transcriptomic trade-offs in both sensory and immune systems after long-term transgenerational exposure to OA. Specifically, RNA-Seq analysis of the olfactory rosette, the peripheral olfactory organ, from 18-months-old F2 revealed extensive regulation in genes involved in ion transport and neuronal signalling, including GABAergic signalling. We also detected OA-induced up-regulation of genes associated with odour transduction, synaptic plasticity, neuron excitability and wiring and down-regulation of genes involved in energy metabolism. Furthermore, OA-exposure induced up-regulation of genes involved in innate antiviral immunity (pathogen recognition receptors and interferon-stimulated genes) in combination with down-regulation of the protein biosynthetic machinery. Consistently, OA-exposed F2 challenged with betanodavirus, which causes damage to the nervous system of marine fish, had acquired improved resistance.
Conclusion: F2 exposed to long-term transgenerational OA acclimation showed superior viral resistance, though as their metabolic and odour transduction programs were altered, odour-mediated behaviours might be consequently impacted. Although it is difficult to unveil how long-term OA impacts propagated between generations, our results reveal that, across generations, trade-offs in plastic responses is a core feature of the olfactory epithelium transcriptome in OA-exposed F2 offspring, and will have important consequences for how cultured and wild fish interacts with its environment.
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Supplement to:
Cohen‐Rengifo, Mishal; Danion, Morgane; Gonzalez, Anne‑Alicia; Bégout, Marie‑Laure; Cormier, Alexandre; Noël, Cyril; Cabon, Joëlle; Vitré, Thomas; Mark, Felix Christopher; Mazurais, David (2022): The extensive transgenerational transcriptomic effects of ocean acidification on the olfactory epithelium of a marine fish are associated with a better viral resistance. BMC Genomics, 23(1), 448, https://doi.org/10.1186/s12864-022-08647-w
Further details:
RNA-seq of D. labrax olfactory rosette - Environmental acidificaton (pH 7.6). National Library of Medicine, https://www.ncbi.nlm.nih.gov/sra/?term=SRR15222852
Cohen‐Rengifo, Mishal; Mouchel, Olivier; Collet, Sophie; Cominassi, Louise; Howald, Sarah; Crespel, Amélie; Cabon, Cabon; Danion, Morgane; Mazurais, David (2022): Partial raw data: sea water carbonate system after years of transgenerational exposure to ocean acidification in the European Sea Bass Dicentrarchus labrax. SEANOE, https://doi.org/10.17882/87395
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html
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Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-09-28.
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License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
8603 data points
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