Rahlff, Janina; Esser, Sarah P; Heinrichs, Mara Elena; Giebel, Helge-Ansgar; Probst, Alexander J (2023): Flow cytometric assessment of virus-like particle, prokaryote and small phototrophic eukaryote abundances from sea foam, surface microlayer, 1-m depth, rainwater from the Swedish Skagerrak (Feb 2020) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.947348
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Abstract:
Flow cytometry samples for analysis of virus-like particle, prokaryote and small phototrophic eukaryote abundances were collected from sea foam, surface microlayer and 1-m deep water from the Swedish Skagerrak during a 1-month campaign at Tjärnö Marine Laboratory in February 2020. Using a small boat, foam and microlayer were collected using a glass plate sampler, and 1-m deep water using a syringe connected to a hose. In addition, rainwater for flow cytometry was collected close to the coast in Duran bottles. Samples were fixed with glutardialdehyde (1% final concentration, Merck, Sweden), stored for 1 hour in the dark and subsequently stored at -80 °C. The purpose of the cellular and viral abundance assessment was to complement metagenomic analyses to investigate viral dispersal across the air-sea interface and along the natural water cycle. In addition, metadata for light conditions, wind speed, salinity, and temperature were recorded. For further details on the campaign and flow cytometric analysis, please refer to Rahlff et al., 2022.
Keyword(s):
Related to:
Rahlff, Janina; Esser, Sarah P; Plewka, Julia; Heinrichs, Mara Elena; Soares, André; Scarchilli, Claudio; Grigioni, Paolo; Wex, Heike; Giebel, Helge-Ansgar; Probst, Alexander J (2023): Marine viruses disperse bidirectionally along the natural water cycle. Nature Communications, 14, 6354, https://doi.org/10.1038/s41467-023-42125-5
Project(s):
Association of European Marine Biological Laboratories Expanded (ASSEMBLE_Plus)
Funding:
European Commission (EC), grant/award no. 730984: Association of European Marine Biological Laboratories Expanded
German Aerospace Center (DLR), grant/award no. 50WB1922: DISPERS - Verbreitung von Mikroorganismen und Viren zur Antarktis mit Auswirkungen auf die Weltraumforschung
German Research Foundation (DFG), grant/award no. 34509606: Ökologie, Physiologie und Molekularbiologie der Roseobacter-Gruppe: Aufbruch zu einem systembiologischen Verständnis einer global wichtigen Gruppe mariner Bakterien
German Research Foundation (DFG), grant/award no. 429483359: NOVAC - Neuartige Viren infizieren Archaeen im terrestrischen Untergrund
German Research Foundation (DFG), grant/award no. 446702140: VIBOCAT - Erforschung des Virioneustons: Viral-bakterielle Interaktionen zwischen Ozean und Atmosphäre
Coverage:
Median Latitude: 58.880887 * Median Longitude: 11.118550 * South-bound Latitude: 58.876092 * West-bound Longitude: 11.105827 * North-bound Latitude: 58.886790 * East-bound Longitude: 11.146365
Date/Time Start: 2020-02-03T12:30:00 * Date/Time End: 2020-02-26T14:00:00
Event(s):
Skagerrak_1 * Latitude: 58.880113 * Longitude: 11.107192 * Date/Time Start: 2020-02-03T12:30:00 * Date/Time End: 2020-02-03T12:45:00 * Method/Device: Multiple investigations (MULT)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Rahlff, Janina | |||
| 2 | Station label | Station | Rahlff, Janina | |||
| 3 | DATE/TIME | Date/Time | Rahlff, Janina | Geocode – start - UTC | ||
| 4 | DATE/TIME | Date/Time | Rahlff, Janina | Geocode – end - UTC | ||
| 5 | Date/Time local | Date/Time local | Rahlff, Janina | start | ||
| 6 | Date/Time local | Date/Time local | Rahlff, Janina | end | ||
| 7 | LATITUDE | Latitude | Rahlff, Janina | Geocode | ||
| 8 | LONGITUDE | Longitude | Rahlff, Janina | Geocode | ||
| 9 | Virus-like particles | Virus-like | #/ml | Rahlff, Janina | Flow cytometer, BD Biosciences, BD Accuri C6 | |
| 10 | Prokaryotes | Prokaryotes | #/ml | Rahlff, Janina | Flow cytometer, BD Biosciences, BD Accuri C6 | |
| 11 | Eukaryotes, phototrophic, small | Euka photo | #/ml | Rahlff, Janina | Flow cytometer, BD Biosciences, BD Accuri C6 | |
| 12 | Habitat | Habitat | Rahlff, Janina | |||
| 13 | Sample method | Sample method | Rahlff, Janina | |||
| 14 | Wind speed, minimum | ff min | m/s | Rahlff, Janina | Anemometer, AN-10, Voltcraft | |
| 15 | Wind speed, maximum | ff max | m/s | Rahlff, Janina | Anemometer, AN-10, Voltcraft | |
| 16 | Brightness | Brightness | lux | Rahlff, Janina | Galaxy Sensors smartphone application v.1.8.10 | Light |
| 17 | Temperature, water | Temp | °C | Rahlff, Janina | Thermosalinometer, portable, WTW Multiline 3420 | |
| 18 | Salinity | Sal | Rahlff, Janina | Thermosalinometer, portable, WTW Multiline 3420 | ||
| 19 | Comment | Comment | Rahlff, Janina | Weather conditions/notes |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
370 data points