Citation:

PANGAEA.947112

Name: Influence of temperature, pCO2, and dissolved nutrients on the oxidative stress response and the carbon metabolism of phytoplankton
Published: 2022-08-16
License: https://creativecommons.org/licenses/by/4.0/
Keywords: carbon metabolism; Diatom; global change; oxidative stress

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Abstract:

We conducted a full-factorial lab experiment to study the individual and combined effects of temperature (18°C and 21°C), pCO2 (400 and 1000 ppm), and dissolved N:P ratio (16 and 25 molar) on the antioxidant capacity and carbon metabolism of the phytoplankton Phaeodactylum tricornutum (strain CCAP 1052/1A). The antioxidant response was assessed based on different biomarkers, including the contents of protective carotenoids (ß-Carotene, diadinoxanthin and violaxanthin), and by determination of antioxidant enzyme activities, and Malondialdehyde (MDA) as a biomarker for oxidative stress. We quantified the activity of Managanese Superoxide Dismutase (SOD-Mn), Glutathione S-transferase (GST), Catalase (CAT), Glutathione Peroxidase (GPx). As possible consequence of oxidative stress on metabolic pathways of carbon, we also quantified carbon fluxes by measuring rates of growth, respiration, dissolved organic carbon (DOC) exudation, and cellular organic carbon content and particulate phosphorus (PP). We also quantified the concentration of photosynthetic pigments chlorophyll a (Chl-a) and fucoxanthin.

Keyword(s):

carbon metabolism; Diatom; global change; oxidative stress

Project(s):

Global change vulnerability of North Sea plankton and associated ecosystem services (PlanktoSERV)

Funding:

Federal Ministry of Education and Research (BMBF), grant/award no. 01LN1702A

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Meunier, Cédric Léo
2	Strain	Strain		Meunier, Cédric Léo		Phytoplankton strain
3	Temperature, water	Temp	°C	Meunier, Cédric Léo
4	Carbon dioxide, partial pressure	pCO2	ppmv	Meunier, Cédric Léo
5	Nitrogen/Phosphorus ratio	N/P		Meunier, Cédric Léo
6	Replicate	Repl		Meunier, Cédric Léo
7	Growth rate	µ	1/day	Meunier, Cédric Léo
8	Carbon per cell	C/cell	pmol/#	Meunier, Cédric Léo
9	Particulate organic phosphorus production per cell	POP prod/cell	pmol/#/day	Meunier, Cédric Léo
10	Respiration rate, carbon	Resp C	pmol/#/day	Meunier, Cédric Léo		respiration per cell
11	Carbon, organic, dissolved exudation, per cell	DOC exud day/cell	pmol/#/day	Meunier, Cédric Léo	TOC analyzer, Shimadzu, TOC-L CPH/CPN
12	Superoxide dismutase manganese activity, unit per protein mass	SODMn/prot	U/mg	Meunier, Cédric Léo	High performance liquid chromatography (HPLC), Agilent, Waters Alliance 2695
13	Glutathione peroxidase activity, unit per protein mass	GPX/prot	U/mg	Meunier, Cédric Léo	High performance liquid chromatography (HPLC), Agilent, Waters Alliance 2695
14	Glutathione S-transferase activity, unit per protein mass	GST/prot	U/mg	Meunier, Cédric Léo	High performance liquid chromatography (HPLC), Agilent, Waters Alliance 2695
15	Catalase activity, unit per protein mass	CAT/prot	U/mg	Meunier, Cédric Léo	High performance liquid chromatography (HPLC), Agilent, Waters Alliance 2695
16	Malondialdehyde, per wet mass	MDA	nmol/g	Meunier, Cédric Léo	High performance liquid chromatography (HPLC), Agilent, Waters Alliance 2695
17	Chlorophyll a per cell	Chl a/cell	pg/#	Meunier, Cédric Léo	High performance liquid chromatography (HPLC), Agilent, Waters Alliance 2695
18	Fucoxanthin per cell	Fuco/cell	pg/#	Meunier, Cédric Léo	High performance liquid chromatography (HPLC), Agilent, Waters Alliance 2695
19	beta-Carotene per cell	b-Car/cell	pg/#	Meunier, Cédric Léo	High performance liquid chromatography (HPLC), Agilent, Waters Alliance 2695
20	Diadinoxanthin per cell	Diadino/cell	pg/#	Meunier, Cédric Léo	High performance liquid chromatography (HPLC), Agilent, Waters Alliance 2695
21	Violaxanthin per cell	Viola/cell	pg/#	Meunier, Cédric Léo	High performance liquid chromatography (HPLC), Agilent, Waters Alliance 2695

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

630 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Study type	2 Strain	3 Temp [°C]	4 pCO2 [ppmv]	5 N/P	6 Repl	7 µ [1/day]	8 C/cell [pmol/#]	9 POP prod/cell [pmol/#/day]	10 Resp C [pmol/#/day]	11 DOC exud day/cell [pmol/#/day]	12 SODMn/prot [U/mg]	13 GPX/prot [U/mg]	14 GST/prot [U/mg]	15 CAT/prot [U/mg]	16 MDA [nmol/g]	17 Chl a/cell [pg/#]	18 Fuco/cell [pg/#]	19 b-Car/cell [pg/#]	20 Diadino/cell [pg/#]	21 Viola/cell [pg/#]
laboratory	CCAP 1052/1A	18	400	16	1	1.2436	1.2168	1.6487	0.2427	-0.6829	5.7523	1.1357	0.5887	0.0252	15.3075	0.1406	0.1320	0.0074	0.0266	0.0033
laboratory	CCAP 1052/1A	18	400	16	2	1.2118	1.1453	1.7780	0.3021	-0.5858	3.6781		0.4248		25.2561	0.1646	0.1541	0.0095	0.0309	0.0033
laboratory	CCAP 1052/1A	18	400	16	3	1.1984	1.2749	2.2499	0.2318	-0.7834	4.1106	5.6397		0.0152		0.1349	0.1314	0.0095	0.0260	0.0034
laboratory	CCAP 1052/1A	18	400	16	4	1.2042	1.2535	2.2997	0.3300	-0.7315		3.7604	0.0606	0.0212	23.2403	0.1438	0.1150	0.0083	0.0258	0.0027
laboratory	CCAP 1052/1A	18	400	25	1	1.2043	1.2179	1.9580	0.2836	-0.6248	32.0533		1.9126		4.3000	0.1287	0.1337	0.0085	0.0213	0.0034
laboratory	CCAP 1052/1A	18	400	25	2	1.2029	1.4018	1.8731	0.2860	-0.2199	50.2042	7.7596	1.1470	0.0000	0.0000	0.1965	0.1699	0.0106	0.0339	0.0038
laboratory	CCAP 1052/1A	18	400	25	3	1.1319	1.3729	2.0708	0.3790	-0.2790	33.5889	28.3781		0.0000		0.1673	0.1652	0.0102	0.0303	0.0038
laboratory	CCAP 1052/1A	18	400	25	4	1.1960	1.2789	2.2825	0.3307	-0.6360		8.0894	0.8514	0.0004	6.1886	0.1125	0.0995	0.0092	0.0221	0.0026
laboratory	CCAP 1052/1A	18	1000	16	1	1.2235	1.3216	1.8881	0.3629	-0.8291		12.1143		0.0811	18.5229	0.1848	0.1526	0.0104	0.0330	0.0031
laboratory	CCAP 1052/1A	18	1000	16	2	1.1970	1.3195	2.0311	0.5008	-0.7753	2.0783		0.6554	0.0581	21.4269	0.1435	0.1424	0.0097	0.0271	0.0034
laboratory	CCAP 1052/1A	18	1000	16	3	1.2304	1.2392	2.2756	0.3886	-0.7198	4.7095	1.9105	0.0318		18.6056	0.1494	0.1203	0.0091	0.0264	0.0028
laboratory	CCAP 1052/1A	18	1000	16	4	1.2531	1.1741	2.1803	0.4715	-0.7160	7.1429	10.6757	0.6575	0.0739		0.1327	0.1214	0.0089	0.0254	0.0029
laboratory	CCAP 1052/1A	18	1000	25	1	1.3005	1.2177	1.8894	0.3074	-0.7306	3.8890		0.4427	0.0032	14.6628	0.1455	0.1404	0.0092	0.0263	0.0025
laboratory	CCAP 1052/1A	18	1000	25	2	1.2420	1.2727	2.1253	0.4116	-0.9010	3.9086	1.7444			38.4901	0.1012	0.1044	0.0083	0.0214	0.0026
laboratory	CCAP 1052/1A	18	1000	25	3	1.2394	1.2854	2.2543	0.4510	-0.6064		19.9662	0.4680	0.0562		0.0941	0.0942	0.0080	0.0189	0.0025
laboratory	CCAP 1052/1A	18	1000	25	4	1.2850		2.2041	0.3969	-0.6672	6.9493	12.0029	0.4365	0.0444	44.7042	0.1333	0.1182	0.0081	0.0249	0.0026
laboratory	CCAP 1052/1A	21	400	16	1	1.3379	0.8568	2.0491	0.2942	0.1983	1.2402	0.6566	0.1086		34.9096	0.1685	0.1383	0.0066	0.0206	0.0027
laboratory	CCAP 1052/1A	21	400	16	2	1.3504	0.9528	2.2613	0.3191	0.1588			0.1106	0.0212	52.7316	0.1548	0.1332	0.0061	0.0207	0.0023
laboratory	CCAP 1052/1A	21	400	16	3	1.2880	0.9510	2.3294	0.3373		1.8205	2.0077	0.0597	0.0179		0.1421	0.1316	0.0063	0.0191	0.0022
laboratory	CCAP 1052/1A	21	400	16	4	1.2548	1.0656	2.5726	0.3150	0.1765	1.4828	3.0816		0.0164	22.7167	0.1325	0.1313	0.0065	0.0180	0.0025
laboratory	CCAP 1052/1A	21	400	25	1	1.3350	0.8696	1.7057	0.3031	0.2836		1.3403	0.1149	0.0155	44.8730	0.1897	0.1610	0.0070	0.0266	0.0029
laboratory	CCAP 1052/1A	21	400	25	2	1.2782	0.9545	2.1668	0.2384	0.4353	0.7953		0.1619		96.7669	0.1755	0.1542	0.0063	0.0230	0.0023
laboratory	CCAP 1052/1A	21	400	25	3	1.2947	0.8920	1.9306	0.2392	0.3097	1.0704	2.8608	0.0784	0.0173		0.1119	0.1330	0.0072	0.0184	0.0026
laboratory	CCAP 1052/1A	21	400	25	4	1.3176	0.9654	2.6017	0.3456	0.2052	0.8637	1.2598		0.0179	54.9690	0.1482	0.1454	0.0066	0.0203	0.0030
laboratory	CCAP 1052/1A	21	1000	16	1	1.2408	0.8902	2.7433	0.2566	0.1977		2.4326	0.0456	0.0326		0.1665	0.1516	0.0068	0.0233	0.0026
laboratory	CCAP 1052/1A	21	1000	16	2	1.2553	0.8536	3.2717	0.2782	0.4945	1.0160		0.0892		54.2982	0.1471	0.1446	0.0064	0.0209	0.0028
laboratory	CCAP 1052/1A	21	1000	16	3	1.2475	0.9643	2.2788	0.2618	0.2730	0.6334	1.9070	0.0853	0.0202	37.7141	0.1604	0.1503	0.0063	0.0222	0.0028
laboratory	CCAP 1052/1A	21	1000	16	4	1.2381	0.8623	2.9574	0.2757	0.2754	1.6133	1.3472		0.0142	43.9961	0.1463	0.1454	0.0064	0.0194	0.0030
laboratory	CCAP 1052/1A	21	1000	25	1	1.2957	0.7501	1.9320	0.1755	0.1959	1.7425	0.3473	0.0977	0.0428		0.1483	0.1450	0.0066	0.0226	0.0023
laboratory	CCAP 1052/1A	21	1000	25	2	1.3300	0.8264	2.3774	0.1814	0.3513	1.6520		0.1148		84.1928	0.1497	0.1426	0.0065	0.0215	0.0023
laboratory	CCAP 1052/1A	21	1000	25	3	1.2423	0.7437	2.2581	0.2212	0.5000	1.3778	1.0315		0.0709	42.2523	0.1793	0.1636	0.0073	0.0255	0.0023
laboratory	CCAP 1052/1A	21	1000	25	4	1.3170	0.8388	2.6936	0.2319	0.6532		2.5187	0.1592	0.0573	20.8216	0.1474	0.1509	0.0067	0.0210	0.0023