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Lymbery, Rowan A; Brouwer, Jill; Evans, Jonathan P (2022): Seawater carbonate chemistry and sperm count and fertilization rate of the mussel Mytilus galloprovincialis [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.945725

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Abstract:
The continued emissions of anthropogenic carbon dioxide are causing progressive ocean acidification (OA). While deleterious effects of OA on biological systems are well documented in the growth of calcifying organisms, lesser studied impacts of OA include potential effects on gamete interactions that determine fertilization, which are likely to influence the many marine species that spawn gametes externally. Here, we explore the effects of OA on the signalling mechanisms that enable sperm to track egg-derived chemicals (sperm chemotaxis). We focus on the mussel Mytilus galloprovincialis, where sperm chemotaxis enables eggs to bias fertilization in favour of genetically compatible males. Using an experimental design based on the North Carolina II factorial breeding design, we test whether the experimental manipulation of seawater pH (comparing ambient conditions to predicted end-of-century scenarios) alters patterns of differential sperm chemotaxis. While we find no evidence that male–female gametic compatibility is impacted by OA, we do find that individual males exhibit consistent variation in how their sperm perform in lowered pH levels. This finding of individual variability in the capacity of ejaculates to respond to chemoattractants under acidified conditions suggests that climate change will exert considerable pressure on male genotypes that can withstand an increasingly hostile fertilization environment.
Keyword(s):
Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Indian Ocean; Laboratory experiment; Mollusca; Mytilus galloprovincialis; Reproduction; Single species; Temperate
Supplement to:
Lymbery, Rowan A; Brouwer, Jill; Evans, Jonathan P (2022): Ocean acidification alters sperm responses to egg-derived chemicals in a broadcast spawning mussel. Biology Letters, 18(4), https://doi.org/10.1098/rsbl.2022.0042
Original version:
Lymbery, Rowan A; Brouwer, Jill; Evans, Jonathan P (2021): Data from: Ocean acidification alters sperm responses to egg-derived chemicals in a broadcast spawning mussel. Dryad, https://doi.org/10.5061/dryad.9cnp5hqkf
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Coverage:
Latitude: -32.234300 * Longitude: 116.273600
Event(s):
Woodman_Point * Latitude: -32.234300 * Longitude: 116.273600 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-06-29.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeLymbery, Rowan AStudy
2Species, unique identificationSpecies UIDLymbery, Rowan A
3Species, unique identification (URI)Species UID (URI)Lymbery, Rowan A
4Species, unique identification (Semantic URI)Species UID (Semantic URI)Lymbery, Rowan A
5BlockBlockLymbery, Rowan AExperimental
6IdentificationIDLymbery, Rowan AFocal male
7IdentificationIDLymbery, Rowan AFocal female
8ReplicateReplLymbery, Rowan ACross
9TreatmentTreatLymbery, Rowan A
10Sperm concentrationSperm#Lymbery, Rowan Aaccumulated after chemotaxis trials
11Sample IDSample IDLymbery, Rowan A
12EggsEggs#Lymbery, Rowan Afertilised
13EggsEggs#Lymbery, Rowan Aunfertilised
14EggsEggs#Lymbery, Rowan Atotal, haphazardly counted
15SalinitySalLymbery, Rowan A
16Temperature, waterTemp°CLymbery, Rowan A
17pHpHLymbery, Rowan APotentiometrictotal scale
18pH, standard errorpH std e±Lymbery, Rowan APotentiometrictotal scale
19Alkalinity, totalATµmol/kgLymbery, Rowan APotentiometric titration
20Alkalinity, total, standard errorAT std e±Lymbery, Rowan APotentiometric titration
21Carbon, inorganic, dissolvedDICµmol/kgLymbery, Rowan ACalculated using seacarb
22Carbon, inorganic, dissolved, standard errorDIC std e±Lymbery, Rowan ACalculated using seacarb
23Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmLymbery, Rowan ACalculated using seacarb
24Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Lymbery, Rowan ACalculated using seacarb
25Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
26Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
15744 data points

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