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Emeis, Kay-Christian; Struck, Ulrich (2022): Alkenone sea surface temperatures, delta13C and delta18O of G. ruber across sapropel intervalses at ODP Sites 967, eastern Mediterranean Sea [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.945377, In: Emeis, K-C; Struck, U (2022): Alkenone sea surface temperatures, delta13C and delta18O of G. ruber across sapropel intervals at ODP Sites 964 and 967, eastern Mediterranean Sea [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.945378

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Published: 2022-06-14DOI registered: 2022-07-23

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Abstract:
Samples were obtained from either those taken aboard JOIDES Resolution during Leg 160 or in the Bremen Ocean Drilling Program (ODP) Core Repository. On the ship, approximately one-quarter of a core in sapropel intervals and the surrounding sediments was sliced off the working halves, wrapped in plastic, and stored frozen until shipping. In the shore-based laboratory, the quarter cores were cut in 1-cm subsamples and freeze dried. Samples taken in the Core Repository consisted of 1-cm sediment intervals across sapropels and adjoining sediments collected with plastic scoops, which were also freeze dried upon arrival in Warnemünde. For each sample, we calculated the revised meters composite depth (rmcd) below seafloor based on the tables provided by Sakamoto et al. (1998).
The freeze-dried samples (~2-4 g dry weight) were subsequently split into three subsamples. Two grams were wet sieved to obtain the >125-μm fraction, and the residue was used to pick up to 20 tests (sometimes samples were barren or contained less than 20 tests) of the planktonic foraminifer Globigerinoides ruber (white) for isotope analyses. This species is a shallow-dwelling planktonic foraminifer and the isotope signal of the calcite tests records global ice-volume variations, salinity, and temperature near the sea surface. The isotopic composition of oxygen and carbon in the calcite was analyzed with a Finigan MAT 251 mass spectrometer, and the measurement was calibrated against the Peedee Belemnite (PDB) reference scale through international isotope standards. The isotopic results are expressed in the usual notation delta = (Rs/Rst -1) * 1000, with Rs and Rst denoting the 13C/12C or 18O/16O ratios in the sample CO2 and the international standard, respectively. The standard deviation of the isotope analyses is ±0.02‰.
The second subsample (~1-2 g dry weight) was used to extract lipids and to estimate the alkenone unsaturation index , a SST indicator (Brassell et al., 1986; Emeis et al., 1995). Weighted splits of freeze-dried and homogenized sediments were extracted by ultrasonic agitation with 35-mL CH2Cl2 (2 ' 10 min). Elemental sulfur was removed by the addition of copper foil during the extraction. After each extraction step, samples were centrifuged, and the solvent was collected by pipette. The two lipid extracts were combined and dried in a rotary evaporator. The extracts were redissolved in CH2Cl2 and precleaned over a silica gel column (conditioned with 30-mL CH2Cl2) by elution with 14-mL CH2Cl2 and dried again in a rotary evaporator. The extracts were redissolved in n-hexane and subsequently fractionated using high-pressure liquid chromatography (HPLC). The HPLC column, packed with silica gel was MERCK LiChrospher Si 100-5. Four fractions were obtained using 5.5-mL n-hexane, 14-mL n-hexane/dichloromethane, 9-mL dichloromethane, and 9-mL acetone as eluents. The ketones (here: alkenones) were isolated in the second fraction. The first fraction contained the aliphatic hydrocarbons, and the third and fourth fractions yielded the more polar compounds.
Gas chromatographic (GC) analyses were carried out on a HR 8000 Fisons gas chromatograph (FID, cold on-column injection) equipped with a 30-m (0.32 mmÆ) glass capillary column (DB5HT). Helium was used as a carrier gas (column head pressure 110 kPa). Oven temperature programming conditions were from 35°C to 300°C at 15°C min-1 followed by an isothermal period of 15 min at 300°C and from 300°C to 330°C at 15°C min-1 followed by an isothermal period of 10 min at 330°C. Alkenones were identified by comparing retention times with those of synthetic standards. Peak areas were converted to the UK'37 index, and SSTs calculated according to a calibration based on culture experiments (Prahl et al., 1988): SST (°C) = (C37:2 / (C37:2 + C37:3) - 0.39) / 0.034.
Alkenone concentrations were determined from GC analyses of the ketone fractions based on peak responses relative to 5alpha-cholestane as an external standard. Results of triplicate analyses (n = 4) indicate that the standard deviation of temperatures calculated from UK'37 is 0.2°C (range 0°C–0.3°C).
The third subsample was used to determine concentrations of organic carbon, total carbon, and total nitrogen. The total carbon and nitrogen contents were determined by combustion at 1200°C under oxygen and detection of CO2 in an infrared detector using a Metalyt CS 100/1000S and a Heraeus elemental analyzer. Inorganic carbon from carbonates was determined in the same instrument by acidifying subsamples with 17% phosphoric acid under a helium stream and detecting evolving CO2. Organic carbon was the difference between total and inorganic carbon. Because of limited sample material, we were not able to perform all analyses on all sapropels.
Keyword(s):
Eastern Mediterranean Sea; Sea surface temperature; stable oxygen and carbon isotopes
Related to:
Brassell, Simon C; Eglinton, Geoffrey; Marlowe, I T; Pflaumann, Uwe; Sarnthein, Michael (1986): Molecular stratigraphy: a new tool for climatic assessment. Nature, 320(6058), 129-133, https://doi.org/10.1038/320129a0
Emeis, Kay-Christian; Anderson, David M; Doose, Heidi; Kroon, Dick; Schulz-Bull, Detlef E (1995): Sea-Surface Temperatures and the History of Monsoon Upwelling in the Northwest Arabian Sea during the Last 500,000 Years. Quaternary Research, 43(3), 355-361, https://doi.org/10.1006/qres.1995.1041
Emeis, Kay-Christian; Schulz, Hans-Martin; Struck, Ulrich; Sakamoto, Tatsuhiko; Doose, Heidi; Erlenkeuser, Helmut; Howell, M W; Kroon, Dick; Paterne, Martine (accepted): Stable isotope and alkenone temperature records of sapropels from Sites 964 and 967: constraining the physical environment of sapropel formation in the Eastern Mediterranean Sea. In: Robertson, A.H.F.; Emeis, K.-C.; Richter, C.; Camerlenghi, A. (eds.), Proceedings of the Ocean Drilling Program, 160 Scientific Results, Proceedings of the Ocean Drilling Program, 160, Ocean Drilling Program, https://doi.org/10.2973/odp.proc.sr.160.011.1998
Prahl, Frederick G; Muehlhausen, Laurel A; Zahnle, Debra L (1988): Further evaluation of long-chain alkenones as indicators of paleoceanographic conditions. Geochimica et Cosmochimica Acta, 52(9), 2303-2310, https://doi.org/10.1016/0016-7037(88)90132-9
Sakamoto, Tatsuhiko; Janecek, Thomas R; Emeis, Kay-Christian (1998): Continuous sedimentary sequences from the Eastern Mediterranean Sea: composite depth sections. In: Robertson, AHF; Emeis, K-C; Richter, C; Camerlenghi, A (eds.), Proceedings of the Ocean Drilling Program, Scientific Results, 160: College Station, TX (Ocean Drilling Program), 160, 37-59, https://doi.org/10.2973/odp.proc.sr.160.053.1998
Project(s):
Coverage:
Median Latitude: 34.070067 * Median Longitude: 32.725500 * South-bound Latitude: 34.068300 * West-bound Longitude: 32.725300 * North-bound Latitude: 34.071100 * East-bound Longitude: 32.725600
Date/Time Start: 1995-04-01T23:50:00 * Date/Time End: 1995-04-03T21:15:00
Minimum DEPTH, sediment/rock: 0.00 m * Maximum DEPTH, sediment/rock: 31.39 m
Event(s):
160-967A * Latitude: 34.068300 * Longitude: 32.725300 * Date/Time Start: 1995-04-01T23:50:00 * Date/Time End: 1995-04-02T11:40:00 * Elevation: -2553.0 m * Penetration: 141.3 m * Recovery: 142.14 m * Location: Eastern Basin * Campaign: Leg160 * Basis: Joides Resolution * Method/Device: Drilling/drill rig (DRILL) * Comment: 16 cores; 141.3 m cored; 0 m drilled; 100.6 % recovery
160-967C * Latitude: 34.071100 * Longitude: 32.725600 * Date/Time Start: 1995-04-03T00:40:00 * Date/Time End: 1995-04-03T11:50:00 * Elevation: -2553.0 m * Penetration: 114.4 m * Recovery: 119.55 m * Location: Eastern Basin * Campaign: Leg160 * Basis: Joides Resolution * Method/Device: Drilling/drill rig (DRILL) * Comment: 13 cores; 114.4 m cored; 0 m drilled; 104.5 % recovery
160-967D * Latitude: 34.070800 * Longitude: 32.725600 * Date/Time Start: 1995-04-03T13:50:00 * Date/Time End: 1995-04-03T21:15:00 * Elevation: -2552.0 m * Penetration: 16.2 m * Recovery: 16.89 m * Location: Eastern Basin * Campaign: Leg160 * Basis: Joides Resolution * Method/Device: Drilling/drill rig (DRILL) * Comment: 2 cores; 16.2 m cored; 0 m drilled; 104.3 % recovery
Comment:
#999 denotes samples with no formainifer tests
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2942 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Sample label

Depth sed [m]

Depth comp r [rmcd]

G. ruber δ13C [‰ PDB]

G. ruber w δ13C std dev [±]

G. ruber δ18O [‰ PDB]

G. ruber w δ18O std dev [±]

UK'37

SST [°C]
10 
C37:2 + C37:3 [ng/g]
11 
TOC [%]
12 
TIC [%]
160-967D-1-1,0-20.000.000.230.28
160-967D-1-1,4-50.040.040.77221.6159
160-967D-1-1,5-70.060.060.16-0.60
160-967D-1-1,9-100.090.09-0.930.84623.744
160-967D-1-1,10-120.110.110.240.73
160-967D-1-1,24-250.240.240.73520.542
160-967D-1-1,29-300.290.290.390.69319.27
160-967D-1-1,34-350.340.340.80422.527
160-967D-1-1,39-400.390.390.76521.450
160-967D-1-1,44-450.440.440.67618.714
160-967D-1-1,45-470.460.460.340.49
160-967D-1-1,49-500.490.49-0.870.68419.013
160-967D-1-1,59-600.590.590.62817.310
160-967D-1-1,64-650.640.640.47412.867
160-967D-1-1,65-670.660.660.600.09
160-967D-1-1,69-700.690.69-0.320.67318.627
160-967D-1-1,70-720.710.710.500.15
160-967D-1-1,74-750.740.740.51714.111
160-967D-1-1,75-770.760.760.31-0.48
160-967D-1-1,79-800.790.790.67118.67
160-967D-1-1,80-820.810.810.48-0.27
160-967D-1-1,84-850.840.840.68118.97
160-967D-1-1,89-900.890.890.70519.68
160-967D-1-1,94-950.940.940.70819.787
160-967D-1-1,99-1000.990.990.75921.2132
160-967D-1-1,100-1011.001.001.100.19-0.300.240.72220.125
160-967D-1-1,101-1021.011.010.76521.455
160-967D-1-1,102-1031.021.021.100.020.230.030.69919.429
160-967D-1-1,103-1041.031.031.130.010.230.020.59516.46
160-967D-1-1,104-1051.041.040.69119.234
160-967D-1-1,104-1051.041.041.160.020.220.050.46812.610
160-967D-1-1,105-1061.051.051.160.02-0.110.040.61617.014
160-967D-1-1,106-1071.061.061.310.01-0.030.030.55615.2
160-967D-1-1,107-1081.071.071.530.01-0.110.020.65318.110
160-967D-1-1,108-1091.081.081.400.01-0.430.020.60416.63
160-967D-1-1,109-1101.091.090.67418.7825
160-967D-1-1,109-1101.091.091.090.02-0.290.020.57315.7
160-967D-1-1,110-1111.101.101.400.01-0.550.030.64417.819
160-967D-1-1,111-1121.111.111.200.02-0.280.020.65018.0118
160-967D-1-1,112-1131.121.121.300.010.060.020.65018.0305
160-967D-1-1,113-1141.131.131.030.01-0.060.030.64717.9262
160-967D-1-1,114-1151.141.140.70819.7234
160-967D-1-1,114-1151.141.141.100.010.050.030.68118.994
160-967D-1-1,115-1161.151.150.520.02-0.280.020.67718.864
160-967D-1-1,116-1171.161.160.68118.961
160-967D-1-1,117-1181.171.171.300.620.920.590.70319.547
160-967D-1-1,118-1191.181.180.080.010.220.020.65718.2426
160-967D-1-1,119-1201.191.190.66018.31638
160-967D-1-1,119-1201.191.190.840.01-0.270.020.67018.6724
160-967D-1-1,120-1211.201.200.660.020.050.030.67018.6412
160-967D-1-1,121-1221.211.210.600.01-0.100.030.66818.5224
160-967D-1-1,122-1231.221.220.510.02-0.160.020.66318.3483
160-967D-1-1,123-1241.231.230.610.02-0.060.010.66418.4506
160-967D-1-1,124-1251.241.240.68719.11184
160-967D-1-1,124-1251.241.241.080.01-0.380.02
160-967D-1-1,125-1261.251.250.710.02-0.380.030.66018.3302
160-967D-1-1,126-1271.261.261.050.01-0.660.02
160-967D-1-1,127-1281.271.270.490.01-0.050.040.67418.7775
160-967D-1-1,128-1291.281.280.490.02-0.450.02
160-967D-1-1,129-1301.291.290.63917.631
160-967D-1-1,129-1301.291.290.440.01-0.070.020.65718.2553
160-967D-1-1,130-1311.301.300.450.01-0.250.02
160-967D-1-1,131-1321.311.310.530.010.100.020.59916.516
160-967D-1-1,132-1331.321.320.610.020.040.03
160-967D-1-1,133-1341.331.330.480.020.390.040.61617.018
160-967D-1-1,134-1351.341.340.54214.814
160-967D-1-1,134-1351.341.340.660.010.740.02
160-967D-1-1,135-1361.351.351.020.021.210.040.48713.29
160-967D-1-1,139-1401.391.390.51714.135
160-967D-1-1,140-1421.411.410.191.54
160-967D-1-1,144-1451.441.441.64
160-967D-1-1,145-1471.461.460.411.76
160-967D-1-2,5-71.561.560.452.32
160-967D-1-2,14-151.641.640.65018.09
160-967D-1-2,15-171.661.660.602.79
160-967D-1-2,19-201.691.690.46412.526
160-967D-1-2,20-221.711.710.963.17
160-967D-1-2,24-251.741.740.41811.131
160-967D-1-2,25-271.761.760.953.29
160-967D-1-2,29-301.791.793.13
160-967D-1-2,35-371.861.860.713.01
160-967D-1-2,40-421.911.911.073.11
160-967D-1-2,45-471.961.960.862.81
160-967D-1-2,49-501.991.992.65
160-967D-1-2,50-522.012.010.432.09
160-967D-1-2,55-572.062.060.441.46
160-967D-1-2,65-672.162.160.702.77
160-967D-1-2,69-702.192.192.36
160-967D-1-2,70-722.212.210.742.77
160-967D-1-2,80-822.312.310.452.55
160-967D-1-2,85-872.362.360.782.52
160-967D-1-2,89-902.392.392.260.39610.511
160-967D-1-2,90-922.412.410.301.73
160-967D-1-2,100-1022.512.510.682.08
160-967D-1-2,105-1072.562.560.201.45
160-967D-1-2,109-1102.592.592.010.39410.415
160-967D-1-2,110-1122.612.610.400.95
160-967D-1-2,115-1172.662.660.461.39
160-967D-1-2,120-1222.712.710.040.48
160-967D-1-2,125-1272.762.760.501.62
160-967D-1-2,130-1322.812.810.581.60
160-967D-1-2,135-1372.862.860.290.87
160-967D-1-2,144-1452.942.941.20
160-967D-1-2,145-1472.962.960.171.16
160-967D-1-3,120-1214.204.160.770.021.020.030.58716.129
160-967D-1-3,121-1224.214.170.710.030.580.03
160-967D-1-3,122-1234.224.180.740.040.390.030.64317.826
160-967D-1-3,123-1244.234.191.280.010.670.03
160-967D-1-3,124-1254.244.200.960.030.780.030.61016.870
160-967D-1-3,125-1264.254.210.410.550.060.42
160-967D-1-3,126-1274.264.221.070.010.100.020.62117.1140
160-967D-1-3,127-1284.274.230.480.020.080.04
160-967D-1-3,128-1294.284.240.750.01-0.080.020.60816.73387
160-967D-1-3,129-1304.294.250.950.02-0.250.03
160-967D-1-3,130-1314.304.260.790.02-0.270.030.61517.03718
160-967D-1-3,131-1324.314.270.720.02-0.470.04
160-967D-1-3,132-1334.324.280.750.03-0.170.060.65718.21114
160-967D-1-3,133-1344.334.290.680.02-0.140.02
160-967D-1-3,134-1354.344.300.780.02-0.080.020.68018.82188
160-967D-1-3,135-1364.354.310.330.03-0.110.03
160-967D-1-3,136-1374.364.320.350.03-0.190.040.64117.7855
160-967D-1-3,137-1384.374.330.970.020.310.04
160-967D-1-3,138-1394.384.340.710.030.270.050.66418.469
160-967D-1-3,139-1404.394.350.830.03-0.040.03
160-967D-1-3,140-1414.404.360.590.010.120.010.57915.98
160-967D-1-3,141-1424.414.371.380.012.100.02
160-967D-1-3,142-1434.424.380.62317.2
160-967D-1-3,143-1444.434.390.730.020.670.08
160-967D-1-3,144-1454.444.401.280.031.890.070.64317.8
160-967D-1-3,145-1464.454.411.270.022.860.05
160-967D-1-3,146-1474.464.420.57915.9
160-967D-1-3,148-1494.474.431.160.032.960.020.62817.3
160-967D-1-3,149-1504.484.440.700.032.600.08
160-967D-1-3,150-1514.494.451.220.022.920.050.64417.8
160-967D-1-4,80-815.305.240.73520.533
160-967D-1-4,81-825.315.251.030.022.010.04
160-967D-1-4,82-835.325.261.180.012.040.020.74420.720
160-967D-1-4,83-845.335.271.110.020.510.02
160-967D-1-4,84-855.345.280.980.010.370.030.70719.623
160-967D-1-4,85-865.355.290.920.020.220.02
160-967D-1-4,86-875.365.301.010.010.460.030.71720.07
160-967D-1-4,87-885.375.310.440.030.450.02
160-967D-1-4,88-895.385.320.430.020.590.040.69119.213
160-967D-1-4,89-905.395.330.370.020.350.04
160-967D-1-4,90-915.405.340.150.020.870.010.70419.6115
160-967D-1-4,91-925.415.351.050.011.570.02
160-967D-1-4,92-935.425.360.760.011.720.030.69619.3108
160-967D-1-4,93-945.435.370.570.020.590.04
160-967D-1-4,94-955.445.380.740.020.250.030.70919.7588
160-967D-1-4,95-965.455.390.830.010.380.03
160-967D-1-4,96-975.465.400.790.010.310.020.75821.12017
160-967D-1-4,97-985.475.410.950.02-0.100.04
160-967D-1-4,98-995.485.421.050.020.130.030.62717.315
160-967D-1-4,99-1005.495.430.530.010.700.02
160-967D-1-4,100-1015.505.440.960.010.320.040.64517.86
160-967D-1-4,101-1025.515.450.620.030.410.04
160-967D-1-4,102-1035.525.460.860.020.600.040.63417.517
160-967D-1-4,103-1045.535.471.400.010.610.03
160-967D-1-4,104-1055.545.481.180.021.280.020.60016.59
160-967D-1-4,105-1065.555.491.010.041.210.04
160-967D-1-4,106-1075.565.501.150.011.280.020.63117.45
160-967D-1-4,107-1085.575.511.040.021.000.02
160-967D-1-4,108-1095.585.521.160.030.990.020.61416.93
160-967D-1-4,109-1105.595.530.340.020.510.04
160-967D-1-4,110-1115.605.540.830.030.710.020.72120.130
160-967D-1-4,111-1125.615.551.240.010.770.03
160-967D-1-4,112-1135.625.560.920.020.820.020.56115.328
160-967D-1-4,113-1145.635.571.110.020.820.04
160-967D-1-4,114-1155.645.580.990.040.920.050.64817.952
160-967D-1-4,115-1165.655.590.830.020.310.04
160-967D-1-4,116-1175.665.600.890.02-0.010.040.69819.42394
160-967D-1-4,117-1185.675.610.230.02-0.360.02
160-967D-1-4,118-1195.685.620.180.03-0.370.030.65818.22266
160-967D-1-4,119-1205.695.630.620.02-1.190.05
160-967D-1-4,120-1215.705.640.630.02-1.110.030.63417.5497
160-967D-1-4,121-1225.715.650.970.011.220.03
160-967D-1-4,122-1235.725.661.030.021.460.030.71820.076
160-967D-1-4,123-1245.735.670.740.021.740.01
160-967D-1-4,124-1255.745.681.240.021.750.020.71619.974
160-967C-1-5,68-696.687.361.260.021.600.030.76021.2466
160-967C-1-5,69-706.697.370.450.01-0.110.03
160-967C-1-5,70-716.707.380.750.01-0.070.03
160-967C-1-5,71-726.717.390.530.02-0.140.030.73320.447
160-967C-1-5,72-736.727.400.680.02-0.230.03
160-967C-1-5,73-746.737.410.390.01-0.430.030.81122.7720
160-967C-1-5,74-756.747.42-0.160.02-0.030.05
160-967C-1-5,75-766.757.430.060.03-0.030.030.81822.97728
160-967C-1-5,76-776.767.44-0.200.03-0.480.04
160-967C-1-5,77-786.777.45-1.050.02-0.810.040.81122.74400
160-967C-1-5,78-796.787.46-1.060.01-1.000.03
160-967C-1-5,79-806.797.47-0.680.03-0.380.040.79022.110203
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