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Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Carapace material properties of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.945344, In: Lowder, K et al. (2022): Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.945362

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Abstract:
We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.2687516) in October 2016 and held at ambient conditions (pH 7.97, 16.5℃) before exposure to stable or diurnally fluctuating reduced pH conditions established by bubbling CO2 and as measured using best practices (ambient pH/stable, 7.97, 16.5℃; reduced pH/stable 7.67, 16.6℃; reduced pH with low fluctuations, 7.67 ± 0.05, 16.4℃; reduced pH with high fluctuations, 7.67 ± 0.10, 16.4℃). After three months, the carapace spine and rostral horn tip were tested for hardness and stiffness using a nanoindentation materials testing machine (Nano Hardness Tester, Nanovea, Irvine, CA, USA) equipped with a Berkovich tip. Fresh samples (<12 hours, except for two samples tested within 24 hours) were kept hydrated in seawater until testing. Samples were secured to an aluminum block with cyanoacrylate glue such that the outer surface was facing up. Indentations were performed by applying a load of 40 mN to the outer surface of the sample at loading and unloading rates of 80 mN/min with a 30 sec hold for creep. At least three indents were taken per sample.
Keyword(s):
California Current Ecosystem; crustacean; Laboratory experiment; Ocean acidification
Supplement to:
Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification-like conditions. Frontiers in Marine Science, 9, 909017, https://doi.org/10.3389/fmars.2022.909017
Related to:
Lowder, Kaitlyn (2022): kblowder/Spiny-lobster-exoskeleton-OA: code only. Zenodo, https://doi.org/10.5281/zenodo.6596558
Coverage:
Latitude: 32.853419 * Longitude: -117.268752
Date/Time Start: 2016-10-01T00:00:00 * Date/Time End: 2016-10-01T00:00:00
Event(s):
La_Jolla_trap_2016 * Latitude: 32.853419 * Longitude: -117.268752 * Date/Time: 2016-10-01T00:00:00 * Location: off Southern California * Method/Device: Trap, baited (TRAPB)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Type of studyStudy typeLowder, Kaitlyn
2Species, unique identificationSpecies UIDLowder, Kaitlyn
3Species, unique identification (URI)Species UID (URI)Lowder, Kaitlyn
4Species, unique identification (Semantic URI)Species UID (Semantic URI)Lowder, Kaitlyn
5Specimen identificationSpec IDLowder, Kaitlyn
6StatusStatusLowder, Kaitlynmolted?
7Body regionBody regionLowder, Kaitlyn
8IdentificationIDLowder, Kaitlyntreatment
9TreatmentTreatLowder, Kaitlyn
10Treatment: pHT:pHLowder, Kaitlyn
11pH, standard deviationpH std dev±Lowder, Kaitlyn
12Treatment: temperatureT:temp°CLowder, Kaitlyn
13Run NumberRun#Lowder, Kaitlyn
14HardnessHardnessGPaLowder, KaitlynNanoindentation (Nano Hardness Tester, Nanovea, Irvine, CA, USA)
15StiffnessStiffnessGPaLowder, KaitlynNanoindentation (Nano Hardness Tester, Nanovea, Irvine, CA, USA)
16Validation flag/commentFlag commLowder, KaitlynHardness in interquartile range?
17Validation flag/commentFlag commLowder, KaitlynStiffness in interquartile range?
18CommentCommentLowder, Kaitlyn
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
4480 data points

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