Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Cuticle elemental concentrations of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.945342, In: Lowder, K et al. (2022): Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.945362
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Abstract:
We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.2687516) in October 2016 and held at ambient conditions (pH 7.97, 16.5°C) before exposure to stable or diurnally fluctuating reduced pH conditions established by bubbling CO2 and as measured using best practices (ambient pH/stable, 7.97, 16.5°C; reduced pH/stable 7.67, 16.6°C; reduced pH with low fluctuations, 7.67 ± 0.05, 16.4°C; reduced pH with high fluctuations, 7.67 ± 0.10, 16.4°C). After three months, inductively-coupled x-ray spectrometry (ICP-MS) was performed on cuticle samples at the Scripps Isotope Geochemistry Laboratory (SIGL) for a precise quantification of elements (µmol/mg sample). The carapace spine was air-dried and then trimmed so only the spine remained with no setae, and the abdominal segment was cut as a 1 x 1 cm² from the center of the second abdominal segment and air-dried. Samples were weighed and placed in Teflon vials for digestion with 0.5 ml of concentrated Teflon-distilled (TD) nitric acid (HNO3) on a hotplate at 100°C for >24 h. Samples were dried down and diluted by a factor of 4000 with 2% TD HNO3 before being transferred to pre-cleaned centrifuge tubes for analysis. Samples were doped with an indium solution to monitor instrumental drift. Measurements were done using a ThermoScientific iCAPq c ICP-MS (Thermo Fisher Scientific GmbH, Bremen, Germany) in standard mode. Masses of Mg and Ca were sequentially measured for 30 ratios, resulting in internal precision of <2% (2 s.d.). Elements were corrected for total mole fraction. Total procedural blanks represented <0.3% of the measurement for Mg and Ca. Raw data were corrected offline for instrument background and drift. Samples were bracketed by internal standards of crab carapace (n=3), which allowed for calculation of absolute values as well as weighted averages of isotopes from natural abundance. The standards yielded external precision of 2% and 3% for Mg and Ca, respectively.
Supplement to:
Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification-like conditions. Frontiers in Marine Science, 9, 909017, https://doi.org/10.3389/fmars.2022.909017
Related to:
Lowder, Kaitlyn (2022): kblowder/Spiny-lobster-exoskeleton-OA: code only. Zenodo, https://doi.org/10.5281/zenodo.6596558
Coverage:
Latitude: 32.853419 * Longitude: -117.268752
Date/Time Start: 2016-10-01T00:00:00 * Date/Time End: 2016-10-01T00:00:00
Event(s):
Parameter(s):
# | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
---|---|---|---|---|---|---|
1 | Type of study | Study type | Lowder, Kaitlyn | |||
2 | Species, unique identification | Species UID | Lowder, Kaitlyn | |||
3 | Species, unique identification (URI) | Species UID (URI) | Lowder, Kaitlyn | |||
4 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Lowder, Kaitlyn | |||
5 | Specimen identification | Spec ID | Lowder, Kaitlyn | |||
6 | Status | Status | Lowder, Kaitlyn | molted? | ||
7 | Body region | Body region | Lowder, Kaitlyn | |||
8 | Identification | ID | Lowder, Kaitlyn | treatment | ||
9 | Treatment | Treat | Lowder, Kaitlyn | |||
10 | Treatment: pH | T:pH | Lowder, Kaitlyn | |||
11 | pH, standard deviation | pH std dev | ± | Lowder, Kaitlyn | ||
12 | Treatment: temperature | T:temp | °C | Lowder, Kaitlyn | ||
13 | Boron-10 | 10B | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
14 | Magnesium-25 | 25Mg | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
15 | Magnesium-26 | 26Mg | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
16 | Magnesium | Mg | µmol/mg | Lowder, Kaitlyn | Weighted average | |
17 | Aluminium-27 | 27Al | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
18 | Phosphorus-31 | 31P | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
19 | Calcium-43 | 43Ca | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
20 | Calcium-48 | 48Ca | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
21 | Calcium | Ca | µmol/mg | Lowder, Kaitlyn | Weighted average | |
22 | Iron-54 | 54Fe | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
23 | Iron-57 | 57Fe | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
24 | Copper-65 | 65Cu | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
25 | Zinc-66 | 66Zn | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
26 | Strontium-86 | 86Sr | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
27 | Barium-137 | 137Ba | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
28 | Lead-208 | 208Pb | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
29 | Uranium-238 | 238U | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
30 | Titanium-48 | 48Ti | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
31 | Chromium-52 | 52Cr | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
32 | Tin-119 | 119Sn | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
33 | Lead-207(I) | 207Pb1 | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
34 | Lead-207(II) | 207Pb2 | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
35 | Lead-207(III) | 207Pb3 | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
36 | Lead-207(IV) | 207Pb4 | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
3776 data points
Download Data
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