Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 deVries Maya S maya.devries@sjsu.edu 0000-0003-1276-1993 Hattingh Ruan ruanhattingh@me.com Day James M D jmdday@ucsd.edu Andersson Andreas J aandersson@ucsd.edu Zerofski Phillip pzerofski@ucsd.edu Taylor Jennifer j3taylor@ucsd.edu 2022 Cuticle relative atomic weight composition of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification dataset https://doi.org/10.1594/PANGAEA.945338 2022-06-13T10:44:27 https://doi.org/10.1594/PANGAEA.945362 We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.2687516) in October 2016 and held at ambient conditions (pH 7.97, 16.5°C) before exposure to stable or diurnally fluctuating reduced pH conditions established by bubbling CO2 and as measured using best practices (ambient pH/stable, 7.97, 16.5°C; reduced pH/stable 7.67, 16.6°C; reduced pH with low fluctuations, 7.67 ± 0.05, 16.4°C; reduced pH with high fluctuations, 7.67 ± 0.10, 16.4°C). After three months, we examined the relative atomic weight composition (weight %/atomic weight of element) of the carapace using a scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX). Each cuticle sample was rinsed with deionized water and allowed to air dry. Samples were then freeze-fractured with liquid nitrogen and critical-point dried (AutoSamdri 815 Series A, Tousimis, Rockville, MD, USA) before being mounted on a 90-degree SEM tip and sputter-coated with iridium. Cross-sections of these cuticle samples were examined with ultra-high-resolution scanning electron microscopy under high vacuum (XL30 SFEG with Sirion column and Apreo LoVac, FEI, Hillsboro, OR, USA with Oxford X-MAX 80 EDS detector, Concord, MA, USA) at 10 or 20 kV. One to two samples each of the carapace spine and antenna from individual lobsters were imaged. EDX was measured with with two machines (XL30 SFEG with Sirion column and Apreo LoVac, FEI, Hillsboro, OR, USA with Oxford X-MAX 80 EDS detector, Concord, MA, USA) at 20 kV acceleration voltage. Spectra were taken on the cross-sectional surface of the exocuticle and the endocuticle layers of the carapace spine and antenna base and the core and outer ring of the horn tip. Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 deVries Maya S maya.devries@sjsu.edu 0000-0003-1276-1993 Hattingh Ruan ruanhattingh@me.com Day James M D jmdday@ucsd.edu Andersson Andreas J aandersson@ucsd.edu Zerofski Phillip pzerofski@ucsd.edu Taylor Jennifer j3taylor@ucsd.edu 2022 Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification-like conditions journal article Frontiers in Marine Science 9 https://doi.org/10.3389/fmars.2022.909017 909017 Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 2022 kblowder/Spiny-lobster-exoskeleton-OA: code only other Zenodo https://doi.org/10.5281/zenodo.6596558 -117.2687516 -117.2687516 32.8534193 32.8534193 -117.2687516 32.8534193 2016-10-01T00:00:00 2016-10-01T00:00:00 not specified La_Jolla_trap_2016 32.8534193 -117.2687516 2016-10-01T00:00:00 off Southern California Trap, baited TRAPB 9733 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Type of study Study type type Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 Study type Species, unique identification Species UID species Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 Species UID Species, unique identification (URI) Species UID (URI) species Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 Species UID (URI) Species, unique identification (Semantic URI) Species UID (Semantic URI) species Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 Species UID (Semantic URI) Specimen identification Spec ID Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 Spec ID Status Status Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 molted? Status Body region Body region Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 Body region Cuticle layer Cuticle layer Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 Cuticle layer Identification ID Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 treatment ID Treatment Treat treatment Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 Treat Treatment: pH T:pH treatment Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 T:pH pH, standard deviation pH std dev ± standard deviation std dev https://en.wikipedia.org/wiki/Standard_deviation Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 pH std dev [±] Treatment: temperature T:temp °C treatment Temperature Θ http://dbpedia.org/resource/Temperature Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 T:temp [°C] Carbon C % carbon C https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:27594 Dimensionless Ratio ψ Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 C [%] Nitrogen N % nitrogen N https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:25555 Dimensionless Ratio ψ Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 N [%] Oxygen O % oxygen O https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:25805 Dimensionless Ratio ψ Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 O [%] Sodium Na % sodium Dimensionless Ratio ψ Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 Na [%] Magnesium Mg % magnesium Dimensionless Ratio ψ Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 Mg [%] Aluminium Al % aluminium Al Dimensionless Ratio ψ Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 Al [%] Silicon Si % silicon Si https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:27573 Dimensionless Ratio ψ Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 Si [%] Phosphorus P % phosphorus Dimensionless Ratio ψ Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 P [%] Sulfur S % sulfur Dimensionless Ratio ψ Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 S [%] Chlorine Cl % chlorine Cl Dimensionless Ratio ψ Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 Cl [%] Calcium Ca % calcium Ca https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:22984 Dimensionless Ratio ψ Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 Ca [%] Device type Device Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 Device Quality control QC Lowder Kaitlyn kaitlyn.b.lowder@gmail.com 0000-0002-8278-5109 QC CurationLevelC Enhanced curation https://wiki.pangaea.de/wiki/Curation_levels citable California Current Ecosystem crustacean Laboratory experiment Ocean acidification author34711 author61591 author61598 author74150 author74151 author74152 author89399 curlevel30 event3017053 geocode1599 geocode1600 geocode1601 journal16757 license21 method11721 method14663 param105163 param174923 param179057 param181311 param18478 param185520 param186962 param26929 param510679 param512410 param512694 param512695 param7056 param74 param75 param78085 param79 param79845 param80 param80167 param815 param87459 param94778 param96278 pi74150 ref103293 ref113244 term23943 term37764 term38477 term38478 term38494 term38520 term38548 term38841 term39985 term40136 term40665 term40685 term41013 term41046 term41048 term43794 term43972 term47269 term49384 term49506 term51590 term51745 topotype1