de.pangaea.dataset945338 und de.pangaea.dataset945362 dataset PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact 2026-04-10T09:57:41Z https://doi.pangaea.de/10.1594/PANGAEA.945338 <gco:CharacterString>Cuticle relative atomic weight composition of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification</gco:CharacterString> 2022-06-13T10:44:27 publication https://doi.org/10.1594/PANGAEA.945338 Lowder, Kaitlyn
kaitlyn.b.lowder@gmail.com
https://orcid.org/0000-0002-8278-5109 information author deVries, Maya S
maya.devries@sjsu.edu
https://orcid.org/0000-0003-1276-1993 information author Hattingh, Ruan
ruanhattingh@me.com
author Day, James M D
jmdday@ucsd.edu
author Andersson, Andreas J
aandersson@ucsd.edu
author Zerofski, Phillip
pzerofski@ucsd.edu
author Taylor, Jennifer
j3taylor@ucsd.edu
author PANGAEA
info@pangaea.de
https://www.pangaea.de/ information publisher tableDigital We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.2687516) in October 2016 and held at ambient conditions (pH 7.97, 16.5°C) before exposure to stable or diurnally fluctuating reduced pH conditions established by bubbling CO2 and as measured using best practices (ambient pH/stable, 7.97, 16.5°C; reduced pH/stable 7.67, 16.6°C; reduced pH with low fluctuations, 7.67 ± 0.05, 16.4°C; reduced pH with high fluctuations, 7.67 ± 0.10, 16.4°C). After three months, we examined the relative atomic weight composition (weight %/atomic weight of element) of the carapace using a scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX). Each cuticle sample was rinsed with deionized water and allowed to air dry. Samples were then freeze-fractured with liquid nitrogen and critical-point dried (AutoSamdri 815 Series A, Tousimis, Rockville, MD, USA) before being mounted on a 90-degree SEM tip and sputter-coated with iridium. Cross-sections of these cuticle samples were examined with ultra-high-resolution scanning electron microscopy under high vacuum (XL30 SFEG with Sirion column and Apreo LoVac, FEI, Hillsboro, OR, USA with Oxford X-MAX 80 EDS detector, Concord, MA, USA) at 10 or 20 kV. One to two samples each of the carapace spine and antenna from individual lobsters were imaged. EDX was measured with with two machines (XL30 SFEG with Sirion column and Apreo LoVac, FEI, Hillsboro, OR, USA with Oxford X-MAX 80 EDS detector, Concord, MA, USA) at 20 kV acceleration voltage. Spectra were taken on the cross-sectional surface of the exocuticle and the endocuticle layers of the carapace spine and antenna base and the core and outer ring of the horn tip. completed Lowder, Kaitlyn
kaitlyn.b.lowder@gmail.com
https://orcid.org/0000-0002-8278-5109 information principalInvestigator California Current Ecosystem crustacean Laboratory experiment Ocean acidification theme <gco:CharacterString>PANGAEA Keyword List</gco:CharacterString> 1995-01-01 creation PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact copyright copyright CC-BY-4.0: Creative Commons Attribution 4.0 International textTable und geoscientificInformation -117.2687516 -117.2687516 32.8534193 32.8534193 2016-10-01T00:00:00 2016-10-01T00:00:00 Type of study thematicClassification Species, unique identification thematicClassification Species, unique identification (URI) thematicClassification Species, unique identification (Semantic URI) thematicClassification Specimen identification thematicClassification Status thematicClassification molted? Body region thematicClassification Cuticle layer thematicClassification Identification thematicClassification treatment Treatment thematicClassification Treatment: pH thematicClassification pH, standard deviation physicalMeasurement ± ± Treatment: temperature physicalMeasurement °C °C Carbon physicalMeasurement Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) % % Nitrogen physicalMeasurement Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) % % Oxygen physicalMeasurement Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) % % Sodium physicalMeasurement Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) % % Magnesium physicalMeasurement Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) % % Aluminium physicalMeasurement Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) % % Silicon physicalMeasurement Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) % % Phosphorus physicalMeasurement Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) % % Sulfur physicalMeasurement Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) % % Chlorine physicalMeasurement Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) % % Calcium physicalMeasurement Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) % % Device type thematicClassification Quality control thematicClassification text/tab-separated-values unknown https://doi.pangaea.de/10.1594/PANGAEA.945338?format=textfile download dataset The data set was checked for completeness, correctness, and consistency of metainformation. Validity of used methods was checked and - if applicable - precision and range of data. EVENT LABEL: La_Jolla_trap_2016 * LATITUDE: 32.853419 * LONGITUDE: -117.268752 * DATE/TIME: 2016-10-01T00:00:00 * LOCATION: off Southern California * METHOD/DEVICE: Trap, baited 2016-10-01T00:00:00 Supplement to <gco:CharacterString>Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification-like conditions</gco:CharacterString> 2022-01-01 publication https://doi.org/10.3389/fmars.2022.909017 Lowder, Kaitlyn
kaitlyn.b.lowder@gmail.com
https://orcid.org/0000-0002-8278-5109 information author deVries, Maya S
maya.devries@sjsu.edu
https://orcid.org/0000-0003-1276-1993 information author Hattingh, Ruan
ruanhattingh@me.com
author Day, James M D
jmdday@ucsd.edu
author Andersson, Andreas J
aandersson@ucsd.edu
author Zerofski, Phillip
pzerofski@ucsd.edu
author Taylor, Jennifer
j3taylor@ucsd.edu
author documentDigital Frontiers in Marine Science 9 909017 Related to <gco:CharacterString>kblowder/Spiny-lobster-exoskeleton-OA: code only</gco:CharacterString> 2022-01-01 publication https://doi.org/10.5281/zenodo.6596558 Lowder, Kaitlyn
kaitlyn.b.lowder@gmail.com
https://orcid.org/0000-0002-8278-5109 information author documentDigital Zenodo