PANGAEA_945338 Cuticle relative atomic weight composition of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer Cuticle relative atomic weight composition of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification 2022-06-13 PANGAEA Dataset https://doi.pangaea.de/10.1594/PANGAEA.945338 Investigator Kaitlyn Lowder kaitlyn.b.lowder@gmail.com Earth Science Type of study Species, unique identification Species, unique identification (URI) Species, unique identification (Semantic URI) Specimen identification Status Body region Cuticle layer Identification Treatment Treatment: pH pH, standard deviation Treatment: temperature Carbon Nitrogen Oxygen Sodium Magnesium Aluminium Silicon Phosphorus Sulfur Chlorine Calcium Device type Quality control geoscientificInformation California Current Ecosystem crustacean La_Jolla_trap_2016 Laboratory experiment Ocean acidification Trap, baited Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) 2016-10-01 2016-10-01 Complete 32.8534193 32.8534193 -117.2687516 -117.2687516 unrestricted CC-BY-4.0: Creative Commons Attribution 4.0 International English PANGAEA Data Publisher for Earth & Environmental Science https://www.pangaea.de/ Data Center Contact Michael Diepenbroek info@pangaea.de

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Bremen Bremen 28359 Germany online 9733 data points text/tab-separated-values Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification-like conditions. Frontiers in Marine Science, 9, 909017, https://doi.org/10.3389/fmars.2022.909017 Lowder, Kaitlyn (2022): kblowder/Spiny-lobster-exoskeleton-OA: code only. Zenodo, https://doi.org/10.5281/zenodo.6596558

We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.2687516) in October 2016 and held at ambient conditions (pH 7.97, 16.5°C) before exposure to stable or diurnally fluctuating reduced pH conditions established by bubbling CO2 and as measured using best practices (ambient pH/stable, 7.97, 16.5°C; reduced pH/stable 7.67, 16.6°C; reduced pH with low fluctuations, 7.67 ± 0.05, 16.4°C; reduced pH with high fluctuations, 7.67 ± 0.10, 16.4°C). After three months, we examined the relative atomic weight composition (weight %/atomic weight of element) of the carapace using a scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX). Each cuticle sample was rinsed with deionized water and allowed to air dry. Samples were then freeze-fractured with liquid nitrogen and critical-point dried (AutoSamdri 815 Series A, Tousimis, Rockville, MD, USA) before being mounted on a 90-degree SEM tip and sputter-coated with iridium. Cross-sections of these cuticle samples were examined with ultra-high-resolution scanning electron microscopy under high vacuum (XL30 SFEG with Sirion column and Apreo LoVac, FEI, Hillsboro, OR, USA with Oxford X-MAX 80 EDS detector, Concord, MA, USA) at 10 or 20 kV. One to two samples each of the carapace spine and antenna from individual lobsters were imaged. EDX was measured with with two machines (XL30 SFEG with Sirion column and Apreo LoVac, FEI, Hillsboro, OR, USA with Oxford X-MAX 80 EDS detector, Concord, MA, USA) at 20 kV acceleration voltage. Spectra were taken on the cross-sectional surface of the exocuticle and the endocuticle layers of the carapace spine and antenna base and the core and outer ring of the horn tip. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.945338"!

https://doi.org/10.3389/fmars.2022.909017 Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification-like conditions https://doi.org/10.5281/zenodo.6596558 kblowder/Spiny-lobster-exoskeleton-OA: code only PANGAEA_945362 DIF 9.4 2022-06-13 2026-04-10