Citation:

PANGAEA.945127

Name: Seawater carbonate chemistry and blood and endolymph acid-base parameters in control and OA-exposed rockfish
Published: 2022-06-08
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Acid-base regulation; Animalia; Bottles or small containers/Aquaria (<20 L); Chordata; Coast and continental shelf; Laboratory experiment; Nekton; North Pacific; Pelagos; Sebastes diploproa; Single species; Temperate

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Abstract:

Over a decade ago, ocean acidification (OA) exposure was reported to induce otolith overgrowth in teleost fish. This phenomenon was subsequently confirmed in multiple species; however, the underlying physiological causes remain unknown. Here, we report that splitnose rockfish (Sebastes diploproa) exposed to 1600 μatm pCO2 (pH 7.5) were able to fully regulated the pH of both blood and endolymph (the fluid that surrounds the otolith within the inner ear). However, while blood was regulated around pH 7.80, the endolymph was regulated around pH 8.30. These different pH setpoints result in increased pCO2 diffusion into the endolymph, which in turn leads to proportional increases in endolymph [HCO3−] and [CO32−]. Endolymph pH regulation despite the increased pCO2 suggests enhanced H+ removal. However, a lack of differences in inner ear bulk and cell-specific Na+/K+-ATPase and vacuolar type H+-ATPase protein abundance localization pointed out to activation of preexisting ATPases, non-bicarbonate pH buffering, or both, as the mechanism for endolymph pH-regulation. These results provide the first direct evidence showcasing the acid-base chemistry of the endolymph of OA-exposed fish favors otolith overgrowth, and suggests that this phenomenon will be more pronounced in species that count with more robust blood and endolymph pH regulatory mechanisms.

Keyword(s):

Acid-base regulation; Animalia; Bottles or small containers/Aquaria (<20 L); Chordata; Coast and continental shelf; Laboratory experiment; Nekton; North Pacific; Pelagos; Sebastes diploproa; Single species; Temperate

Supplement to:

Kwan, Garfield Tsz; Tresguerres, Martin (2022): Elucidating the acid-base mechanisms underlying otolith overgrowth in fish exposed to ocean acidification. Science of the Total Environment, 823, 153690, https://doi.org/10.1016/j.scitotenv.2022.153690

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-06-08.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Kwan, Garfield Tsz		Study
2	Species, unique identification	Species UID		Kwan, Garfield Tsz
3	Species, unique identification (URI)	Species UID (URI)		Kwan, Garfield Tsz
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Kwan, Garfield Tsz
5	Treatment	Treat		Kwan, Garfield Tsz
6	Carbon dioxide, partial pressure	pCO2	µatm	Kwan, Garfield Tsz		Blood
7	Carbon dioxide, partial pressure, standard error	pCO2 std e	±	Kwan, Garfield Tsz		Blood
8	Carbon dioxide	CO2	mmol/l	Kwan, Garfield Tsz		Blood
9	Carbon dioxide, standard error	CO2 std e	±	Kwan, Garfield Tsz		Blood
10	Hydrogen ion concentration	H+	nmol/l	Kwan, Garfield Tsz		Blood
11	Hydrogen ion concentration, standard error	H+ std e	±	Kwan, Garfield Tsz		Blood
12	pH	pH		Kwan, Garfield Tsz		Blood
13	pH, standard error	pH std e	±	Kwan, Garfield Tsz		Blood
14	Bicarbonate	[HCO3]-	mmol/l	Kwan, Garfield Tsz		Blood
15	Bicarbonate ion, standard error	[HCO3]- std e	±	Kwan, Garfield Tsz		Blood
16	Carbonate ion	[CO3]2-	µmol/l	Kwan, Garfield Tsz		Blood
17	Carbonate ion, standard error	[CO3]2- std e	±	Kwan, Garfield Tsz		Blood
18	Carbon, inorganic, dissolved	DIC	mmol/l	Kwan, Garfield Tsz		Blood
19	Carbon, inorganic, dissolved, standard error	DIC std e	±	Kwan, Garfield Tsz		Blood
20	Replicates	Repl	#	Kwan, Garfield Tsz		Blood
21	Carbon dioxide, partial pressure	pCO2	µatm	Kwan, Garfield Tsz		Endolymph
22	Carbon dioxide, partial pressure, standard error	pCO2 std e	±	Kwan, Garfield Tsz		Endolymph
23	Carbon dioxide	CO2	mmol/l	Kwan, Garfield Tsz		Endolymph
24	Carbon dioxide, standard error	CO2 std e	±	Kwan, Garfield Tsz		Endolymph
25	Hydrogen ion concentration	H+	nmol/l	Kwan, Garfield Tsz		Endolymph
26	Hydrogen ion concentration, standard error	H+ std e	±	Kwan, Garfield Tsz		Endolymph
27	pH	pH		Kwan, Garfield Tsz		Endolymph
28	pH, standard error	pH std e	±	Kwan, Garfield Tsz		Endolymph
29	Bicarbonate	[HCO3]-	mmol/l	Kwan, Garfield Tsz		Endolymph
30	Bicarbonate ion, standard error	[HCO3]- std e	±	Kwan, Garfield Tsz		Endolymph
31	Carbonate ion	[CO3]2-	µmol/l	Kwan, Garfield Tsz		Endolymph
32	Carbonate ion, standard error	[CO3]2- std e	±	Kwan, Garfield Tsz		Endolymph
33	Carbon, inorganic, dissolved	DIC	mmol/l	Kwan, Garfield Tsz		Endolymph
34	Carbon, inorganic, dissolved, standard error	DIC std e	±	Kwan, Garfield Tsz		Endolymph
35	Replicates	Repl	#	Kwan, Garfield Tsz		Endolymph
36	Proteins	Protein		Kwan, Garfield Tsz		NKA
37	Proteins, standard error	Protein std e	±	Kwan, Garfield Tsz		NKA
38	Proteins	Protein		Kwan, Garfield Tsz		VHA
39	Proteins, standard error	Protein std e	±	Kwan, Garfield Tsz		VHA
40	pH	pH		Kwan, Garfield Tsz	Spectrophotometric	total scale
41	pH, standard error	pH std e	±	Kwan, Garfield Tsz	Spectrophotometric	total scale
42	Alkalinity, total	AT	µmol/kg	Kwan, Garfield Tsz	Potentiometric titration
43	Alkalinity, total, standard error	AT std e	±	Kwan, Garfield Tsz	Potentiometric titration
44	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Kwan, Garfield Tsz	Calculated using CO2SYS
45	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Kwan, Garfield Tsz	Calculated using CO2SYS
46	Salinity	Sal		Kwan, Garfield Tsz
47	Salinity, standard error	Sal std e	±	Kwan, Garfield Tsz
48	Temperature, water	Temp	°C	Kwan, Garfield Tsz
49	Temperature, water, standard error	T std e	±	Kwan, Garfield Tsz
50	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
54	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
55	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
56	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
57	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
58	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

112 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (Study)	2 Species UID	3 Species UID (URI)	4 Species UID (Semantic URI)	5 Treat	6 pCO2 [µatm] (Blood)	7 pCO2 std e [±] (Blood)	8 CO2 [mmol/l] (Blood)	9 CO2 std e [±] (Blood)	10 H+ [nmol/l] (Blood)	11 H+ std e [±] (Blood)	12 pH (Blood)	13 pH std e [±] (Blood)	14 [HCO3]- [mmol/l] (Blood)	15 [HCO3]- std e [±] (Blood)	16 [CO3]2- [µmol/l] (Blood)	17 [CO3]2- std e [±] (Blood)	18 DIC [mmol/l] (Blood)	19 DIC std e [±] (Blood)	20 Repl [#] (Blood)	21 pCO2 [µatm] (Endolymph)	22 pCO2 std e [±] (Endolymph)	23 CO2 [mmol/l] (Endolymph)	24 CO2 std e [±] (Endolymph)	25 H+ [nmol/l] (Endolymph)	26 H+ std e [±] (Endolymph)	27 pH (Endolymph)	28 pH std e [±] (Endolymph)	29 [HCO3]- [mmol/l] (Endolymph)	30 [HCO3]- std e [±] (Endolymph)	31 [CO3]2- [µmol/l] (Endolymph)	32 [CO3]2- std e [±] (Endolymph)	33 DIC [mmol/l] (Endolymph)	34 DIC std e [±] (Endolymph)	35 Repl [#] (Endolymph)	36 Protein (NKA)	37 Protein std e [±] (NKA)	38 Protein (VHA)	39 Protein std e [±] (VHA)	40 pH (total scale, Spectrophotometric)	41 pH std e [±] (total scale, Spectrophotometric)	42 AT [µmol/kg] (Potentiometric titration)	43 AT std e [±] (Potentiometric titration)	44 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	45 pCO2water_SST_wet std e [±] (Calculated using CO2SYS)	46 Sal	47 Sal std e [±]	48 Temp [°C]	49 T std e [±]	50 CSC flag (Calculated using seacarb afte...)	51 CO2 [µmol/kg] (Calculated using seacarb afte...)	52 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	53 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	54 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	55 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	56 DIC [µmol/kg] (Calculated using seacarb afte...)	57 Omega Arg (Calculated using seacarb afte...)	58 Omega Cal (Calculated using seacarb afte...)
laboratory	Sebastes diploproa	marinespecies.org	urn:lsid:marinespecies.org:taxname:274787	Control	1603.25	190.69	0.07	0.01	17.69	1.38	7.75	0.03	2.37	0.20	20	0	2.46	0.20	8	971.38	120.70	0.06	0.01	5.04	0.65	8.32	0.05	8.63	0.35	0.36	0.04	9.06	0.38	7	1.00	0.12	1.00	0.08	7.89	0.01	2229.65	1.62	571.90	4.88	33.27	0.04	15.83	0.003	8	21.81	591.25	593.38	1953.04	111.68	2086.53	1.73	2.69
laboratory	Sebastes diploproa	marinespecies.org	urn:lsid:marinespecies.org:taxname:274787	OA	2659.20	223.87	0.12	0.01	14.50	1.29	7.85	0.04	5.16	0.31	70	10	5.34	0.32	9	1503.21	73.72	0.10	0.00	4.50	0.18	8.35	0.02	15.19	0.95	0.67	0.06	15.96	1.02	8	0.98	0.15	0.78	0.13	7.49	0.01	2230.33	3.27	1591.56	18.58	33.27	0.04	15.83	0.003	8	59.23	1605.52	1611.29	2111.31	48.06	2218.60	0.74	1.16