Citation:

PANGAEA.944572

Name: Seawater carbonate chemistry and drug concentrations in tissues, bioconcentration factor and enzymatic activity of Ruditapes philippinarum
Published: 2022-05-25
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Brackish waters; Laboratory experiment; Mollusca; North Atlantic; Organic toxins; Other metabolic rates; Other studied parameter or process; Ruditapes philippinarum; Single species; Temperate

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Abstract:

In coastal systems, organisms are exposed to a multitude of stressors whose interactions and effects are poorly studied. Pharmaceutical drugs and Climate Change consequences, such as reduced pH, are examples of stressors affecting marine organisms, as bivalves. Although a vast literature is available for the effects of these stressors when acting individually, very limited information exists on the impacts that the combination of both can have on marine bivalves. For this reason, this study aimed to evaluate the impacts of a simulated ocean acidification scenario (control pH, 8.0; reduced pH, pH 7.6) on the effects of the antiepileptic carbamazepine (CBZ, 1 μg/L) and the antihistamine cetirizine (CTZ, 0.6 μg/L), when acting individually and combined (CBZ + CTZ), on the edible clam Ruditapes philippinarum. After 28 days of exposure, drug concentrations, bioconcentration factors and biochemical parameters related to the clam's metabolic capacity and oxidative stress were evaluated. The results showed that R. philippinarum clams responded differently to pharmaceutical drugs depending on the pH tested, influencing both bioconcentration and biological responses. In general, drug combined treatments showed fewer impacts than drugs acting alone, and acidification seemed to activate at a higher extension the elimination processes that were not activated under control pH. Also, reduced pH per se exerted negative impacts (e.g., cellular damage) on R. philippinarum and the combination with pharmaceutical drugs did not enhance the toxicity.

Keyword(s):

Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Brackish waters; Laboratory experiment; Mollusca; North Atlantic; Organic toxins; Other metabolic rates; Other studied parameter or process; Ruditapes philippinarum; Single species; Temperate

Supplement to:

Almeida, Angela; Calisto, Vania; Esteves, Valdemar I; Schneider, Rudolf J; Soares, Amadeu M V M; Freitas, Rosa (2022): Responses of Ruditapes philippinarum to contamination by pharmaceutical drugs under ocean acidification scenario. Science of the Total Environment, 824, 153591, https://doi.org/10.1016/j.scitotenv.2022.153591

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-05-24.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Freitas, Rosa		study
2	Species, unique identification	Species UID		Freitas, Rosa
3	Species, unique identification (URI)	Species UID (URI)		Freitas, Rosa
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Freitas, Rosa
5	Treatment	Treat		Freitas, Rosa
6	Treatment	Treat		Freitas, Rosa
7	Drug concentration per fresh mass	Drug/fm	ng/g	Freitas, Rosa		in clams tissues
8	Drug concentration per fresh mass, standard deviation	Drug/fm std dev	±	Freitas, Rosa		in clams tissues
9	Bioconcentration factor	BCF	L/kg	Freitas, Rosa
10	Bioconcentration factor, standard deviation	BCF std dev	±	Freitas, Rosa
11	Energy transport system activity, per fresh mass	ETS/fm	nmol/g/min	Freitas, Rosa
12	Electron transport system activity of oyxgen, standard deviation	ETS std dev	±	Freitas, Rosa
13	Glycogen	GLY	µg/mg	Freitas, Rosa
14	Glycogen, standard deviation	GLY std dev	±	Freitas, Rosa
15	Proteins per fresh mass	Prot	mg/g	Freitas, Rosa
16	Proteins, standard deviation	Protein std dev	±	Freitas, Rosa
17	Superoxide dismutase, per fresh mass	SOD/fm	U/g	Freitas, Rosa
18	Superoxide dismutase activity, standard deviation	SOD std dev	±	Freitas, Rosa
19	Catalase activity, per fresh mass	CA/fm	U/g	Freitas, Rosa
20	Catalase activity, standard deviation	CAT std dev	±	Freitas, Rosa
21	Glutathione S-transferase activity, per fresh mass	GST/fm	U/g	Freitas, Rosa
22	Glutathione S-transferase activity, standard deviation	GST std dev	±	Freitas, Rosa
23	Lipid peroxidation, per fresh mass	LPO/fm	nmol/g	Freitas, Rosa
24	Lipid peroxidation, standard deviation	LPO std dev	±	Freitas, Rosa
25	Protein carbonylation, per fresh mass	PC/fm	µmol/g	Freitas, Rosa
26	Protein carbonylation, per fresh mass, standard deviation	PC/fm std dev	±	Freitas, Rosa
27	Glutathione reduced/Glutathione oxidized, ratio	GSH/GSSG		Freitas, Rosa
28	Glutathione reduced/Glutathione oxidized, ratio, standard deviation	GSH/GSSG std dev	±	Freitas, Rosa
29	Temperature, water	Temp	°C	Freitas, Rosa
30	Temperature, water, standard deviation	Temp std dev	±	Freitas, Rosa
31	Salinity	Sal		Freitas, Rosa
32	Salinity, standard deviation	Sal std dev	±	Freitas, Rosa
33	pH	pH		Freitas, Rosa	Potentiometric	NBS scale
34	pH, standard deviation	pH std dev	±	Freitas, Rosa	Potentiometric	NBS scale
35	Alkalinity, total	AT	µmol/kg	Freitas, Rosa	Potentiometric titration
36	Alkalinity, total, standard deviation	AT std dev	±	Freitas, Rosa	Potentiometric titration
37	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Freitas, Rosa	Calculated using CO2SYS
38	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Freitas, Rosa	Calculated using CO2SYS
39	Carbonate ion	[CO3]2-	µmol/kg	Freitas, Rosa	Calculated using CO2SYS
40	Carbonate ion, standard deviation	[CO3]2- std dev	±	Freitas, Rosa	Calculated using CO2SYS
41	Bicarbonate ion	[HCO3]-	µmol/kg	Freitas, Rosa	Calculated using CO2SYS
42	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Freitas, Rosa	Calculated using CO2SYS
43	Calcite saturation state	Omega Cal		Freitas, Rosa	Calculated using CO2SYS
44	Calcite saturation state, standard deviation	Omega Cal std dev	±	Freitas, Rosa	Calculated using CO2SYS
45	Aragonite saturation state	Omega Arg		Freitas, Rosa	Calculated using CO2SYS
46	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Freitas, Rosa	Calculated using CO2SYS
47	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
49	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
54	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
55	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
56	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

464 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species UID	3 Species UID (URI)	4 Species UID (Semantic URI)	5 Treat	6 Treat	7 Drug/fm [ng/g] (in clams tissues)	8 Drug/fm std dev [±] (in clams tissues)	9 BCF [L/kg]	10 BCF std dev [±]	11 ETS/fm [nmol/g/min]	12 ETS std dev [±]	13 GLY [µg/mg]	14 GLY std dev [±]	15 Prot [mg/g]	16 Protein std dev [±]	17 SOD/fm [U/g]	18 SOD std dev [±]	19 CA/fm [U/g]	20 CAT std dev [±]	21 GST/fm [U/g]	22 GST std dev [±]	23 LPO/fm [nmol/g]	24 LPO std dev [±]	25 PC/fm [µmol/g]	26 PC/fm std dev [±]	27 GSH/GSSG	28 GSH/GSSG std dev [±]	29 Temp [°C]	30 Temp std dev [±]	31 Sal	32 Sal std dev [±]	33 pH (NBS scale, Potentiometric)	34 pH std dev [±] (NBS scale, Potentiometric)	35 AT [µmol/kg] (Potentiometric titration)	36 AT std dev [±] (Potentiometric titration)	37 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	38 pCO2 std dev [±] (Calculated using CO2SYS)	39 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	40 [CO3]2- std dev [±] (Calculated using CO2SYS)	41 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	42 [HCO3]- std dev [±] (Calculated using CO2SYS)	43 Omega Cal (Calculated using CO2SYS)	44 Omega Cal std dev [±] (Calculated using CO2SYS)	45 Omega Arg (Calculated using CO2SYS)	46 Omega Arg std dev [±] (Calculated using CO2SYS)	47 CSC flag (Calculated using seacarb afte...)	48 pH (total scale, Calculated using...)	49 CO2 [µmol/kg] (Calculated using seacarb afte...)	50 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	51 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	52 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	53 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	54 DIC [µmol/kg] (Calculated using seacarb afte...)	55 Omega Arg (Calculated using seacarb afte...)	56 Omega Cal (Calculated using seacarb afte...)
laboratory	Ruditapes philippinarum	marinespecies.org	urn:lsid:marinespecies.org:taxname:231750	Control pH	CBZ, single	1.02	0.06	0.98	0.06
laboratory	Ruditapes philippinarum	marinespecies.org	urn:lsid:marinespecies.org:taxname:231750	Control pH	CTZ, single	1.25	0.09	2.31	0.17
laboratory	Ruditapes philippinarum	marinespecies.org	urn:lsid:marinespecies.org:taxname:231750	Control pH	CBZ, combined	0.94	0.12	0.92	0.12
laboratory	Ruditapes philippinarum	marinespecies.org	urn:lsid:marinespecies.org:taxname:231750	Control pH	CTZ, combined	1.11	0.20	2.01	0.37
laboratory	Ruditapes philippinarum	marinespecies.org	urn:lsid:marinespecies.org:taxname:231750	Lowered pH	CBZ, single	1.37	0.21	1.37	0.21
laboratory	Ruditapes philippinarum	marinespecies.org	urn:lsid:marinespecies.org:taxname:231750	Lowered pH	CTZ, single	1.15	0.10	2.18	0.18
laboratory	Ruditapes philippinarum	marinespecies.org	urn:lsid:marinespecies.org:taxname:231750	Lowered pH	CBZ, combined	0.54	0.10	0.53	0.09
laboratory	Ruditapes philippinarum	marinespecies.org	urn:lsid:marinespecies.org:taxname:231750	Lowered pH	CTZ, combined	0.87	0.16	1.88	0.35
laboratory	Ruditapes philippinarum	marinespecies.org	urn:lsid:marinespecies.org:taxname:231750	Control pH	CTL					2.06	0.30	1.84	0.13	18.06	1.03	0.47	0.15	1.92	0.48	0.14	0.04	4.92	0.54	0.16	0.01	0.59	0.01	17.60	0.48	25.67	0.49	8.05	0.01	1203.84	30.21	221.38	6.72	71.18	2.04	1007.52	26.57	1.81	0.05	1.13	0.03	26	7.91	11.61	317.95	319.07	1050.91	55.79	1118.3	0.89	1.42
laboratory	Ruditapes philippinarum	marinespecies.org	urn:lsid:marinespecies.org:taxname:231750	Control pH	CBZ					2.06	0.55	2.82	0.10	20.16	1.97	0.52	0.27	2.58	0.55	0.18	0.02	7.27	0.64	0.17	0.02	0.51	0.05	17.09	0.14	25.42	0.51	8.05	0.01	1148.71	9.46	206.54	3.17	68.65	1.18	956.79	7.45	1.57	0.34	1.09	0.02	26	7.92	11.07	298.39	299.44	1002.70	52.62	1066.4	0.84	1.34
laboratory	Ruditapes philippinarum	marinespecies.org	urn:lsid:marinespecies.org:taxname:231750	Control pH	CTZ					2.75	0.40	2.85	0.31	19.58	0.68	2.00	0.30	3.54	0.46	0.17	0.02	6.78	0.30	0.23	0.01	0.50	0.03	18.17	0.49	25.50	0.52	8.04	0.01	1186.60	33.50	218.09	8.81	70.10	1.77	992.41	30.61	1.78	0.04	1.12	0.03	26	7.91	11.35	315.72	316.82	1033.96	55.60	1100.9	0.89	1.41
laboratory	Ruditapes philippinarum	marinespecies.org	urn:lsid:marinespecies.org:taxname:231750	Control pH	CBZ+CTZ					2.03	0.38	2.15	0.24	19.33	0.86	0.85	0.25	3.28	0.16	0.16	0.02	5.91	0.66	0.18	0.01	0.49	0.07	17.39	0.35	25.42	0.51	8.03	0.01	1157.93	42.13	222.32	12.02	66.17	2.41	973.36	38.46	1.68	0.06	1.05	0.04	26	7.90	11.69	317.74	318.86	1015.72	51.40	1078.8	0.82	1.31
laboratory	Ruditapes philippinarum	marinespecies.org	urn:lsid:marinespecies.org:taxname:231750	Lowered pH	CTL					3.73	0.28	2.04	0.31	20.87	2.66	1.96	0.33	3.64	0.40	0.26	0.04	8.58	0.10	0.20	0.03	0.56	0.04	16.73	0.11	25.42	0.51	7.54	0.02	1239.17	42.03	789.35	27.34	26.08	0.22	1167.89	42.49	0.66	0.01	0.42	0.00	26	7.41	42.43	1130.98	1135.00	1187.23	19.15	1248.8	0.30	0.49
laboratory	Ruditapes philippinarum	marinespecies.org	urn:lsid:marinespecies.org:taxname:231750	Lowered pH	CBZ					4.09	0.52	2.50	0.25	20.69	2.17	2.48	0.62	3.75	0.29	0.18	0.04	9.71	1.13	0.22	0.03	0.55	0.01	17.83	0.38	25.33	0.49	7.57	0.01	1258.29	4.73	772.68	30.99	28.09	0.85	1181.91	6.64	0.71	0.02	0.45	0.01	26	7.44	39.47	1086.33	1090.14	1200.64	21.38	1261.5	0.34	0.54
laboratory	Ruditapes philippinarum	marinespecies.org	urn:lsid:marinespecies.org:taxname:231750	Lowered pH	CTZ					3.98	0.37	2.17	0.43	21.30	1.85	1.69	0.49	2.95	0.54	0.24	0.02	7.41	1.19	0.19	0.04	0.52	0.08	16.37	0.15	25.67	0.49	7.55	0.03	1254.19	27.22	808.93	47.15	26.86	2.46	1181.06	21.45	0.68	0.06	0.43	0.04	26	7.42	42.30	1117.07	1121.05	1200.98	19.62	1262.9	0.31	0.50
laboratory	Ruditapes philippinarum	marinespecies.org	urn:lsid:marinespecies.org:taxname:231750	Lowered pH	CBZ+CTZ					5.17	0.55	3.05	0.47	21.50	2.45	1.97	0.54	3.59	0.27	0.24	0.02	7.39	0.37	0.20	0.01	0.53	0.02	16.73	0.28	25.33	0.49	7.55	0.02	1283.21	31.85	834.12	30.62	27.26	1.69	1209.46	28.54	0.69	0.04	0.43	0.03	26	7.42	43.53	1159.95	1164.06	1229.48	19.99	1293.0	0.32	0.51