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Balaguer, Jenna; Thoms, Silke; Trimborn, Scarlett (2023): Intracellular trace metals content of iron and manganese of Phaeocystis antarctica after exposure to different Fe and Mn availabilities at the end of the experiment [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.944457, In: Balaguer, J et al. (2023): Elemental composition of the Southern Ocean key species Phaeocystis antarctica grown under altered iron and manganese supply [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.944462

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Abstract:
For determination of the cellular trace metal content, at the end of the experiment 400 mL of water were filtered onto 0.2 μm TMC-cleaned polycarbonate filters (EMD Millipore, Darmstadt, Germany) and then rinsed for 15 min with a 0.1 M oxalic acid wash to remove cell surface bound trace metals. Finally, the filters were rinsed with filtered seawater and stored in 5 mL TMC polyfluoralkoxy vials until further analysis. Samples were analyzed with an inductively coupled plasma mass spectrometry (ICP-MS) following a digestion with nitric acid (HNO3) and HF.
Keyword(s):
carbon fixation; Fe:C ratio; growth; iron; manganese; photophysiology; Phytoplankton; Southern Ocean; Trace metal
Funding:
German Research Foundation (DFG), grant/award no. 5472008: Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas
Coverage:
Latitude: -62.237900 * Longitude: -64.557460
Date/Time Start: 2018-04-02T09:11:00 * Date/Time End: 2018-04-02T09:11:00
Minimum Elevation: -2647.0 m * Maximum Elevation: -2647.0 m
Event(s):
PS112_26-1 * Latitude: -62.237900 * Longitude: -64.557460 * Date/Time: 2018-04-02T09:11:00 * Elevation: -2647.0 m * Location: Drake Passage * Campaign: PS112 (ANT-XXXIII/3) * Basis: Polarstern * Method/Device: In situ pump (ISP) * Comment: Trace metal sea water pump from a depth of 25 m
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TreatmentTreatBalaguer, Jenna
2Manganese, cellular quotaQMnamol/#Balaguer, JennaInductively coupled plasma mass spectrometry (ICP-MS)
3Iron, cellular quotaQFeamol/#Balaguer, JennaInductively coupled plasma mass spectrometry (ICP-MS)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
48 data points

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