Balaguer, Jenna; Thoms, Silke; Trimborn, Scarlett (2023): Cell growth and Particulate Organic Carbon / Nitrogen content of Phaeocystis antarctica after exposure to different Fe and Mn availabilities at the end of the experiment [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.944456, In: Balaguer, J et al. (2023): Elemental composition of the Southern Ocean key species Phaeocystis antarctica grown under altered iron and manganese supply [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.944462
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Abstract:
Cell counts and POC/PON content of P.antartica for the 4 different treatments were determined at the beginning and at the end of the experiment. The cell density and size were determined using a Beckman MultisizerTM 3 Coulter Counter® with a 100 μm aperture. For the POC/PON, 250 mL of water were filtered onto pre-combusted glass-fiber filters (15h, 500 ̊C, GF/F, ~0.6 μm, 25 mm, Whatman, Wisconsin, USA) and stored at -20°C in pre-combusted glass petri dishes. Prior to analysis with an Euro Elemental Analyzer 3000 CHNS-O (HEKAtech GmbH), the filters were dried for > 12h at 60 °C and then acidified with 200 μL of 0.2N HCL to remove inorganic carbon and dried a second time. Contents of POC and PON were corrected for blank measurements and normalized to filtered volume and cell densities to yield cellular quotas.
Keyword(s):
Funding:
German Research Foundation (DFG), grant/award no. 5472008: Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas
Coverage:
Latitude: -62.237900 * Longitude: -64.557460
Date/Time Start: 2018-04-02T09:11:00 * Date/Time End: 2018-04-02T09:11:00
Minimum Elevation: -2647.0 m * Maximum Elevation: -2647.0 m
Event(s):
PS112_26-1 * Latitude: -62.237900 * Longitude: -64.557460 * Date/Time: 2018-04-02T09:11:00 * Elevation: -2647.0 m * Location: Drake Passage * Campaign: PS112 (ANT-XXXIII/3) * Basis: Polarstern * Method/Device: In situ pump (ISP) * Comment: Trace metal sea water pump from a depth of 25 m
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Treatment | Treat | Balaguer, Jenna | |||
| 2 | Phaeocystis antarctica | P. antarctica | #/l | Balaguer, Jenna | Coulter counter, Beckman Coulter, Multisizer 3 | |
| 3 | Carbon, organic, particulate | POC | mg/l | Balaguer, Jenna | Element analyser Euro EA 3000 | 1 |
| 4 | Carbon, organic, particulate | POC | mg/l | Balaguer, Jenna | Element analyser Euro EA 3000 | 2 |
| 5 | Nitrogen, organic, particulate | PON | mg/l | Balaguer, Jenna | Element analyser Euro EA 3000 | 1 |
| 6 | Nitrogen, organic, particulate | PON | mg/l | Balaguer, Jenna | Element analyser Euro EA 3000 | 2 |
| 7 | Experiment day | Exp day | day | Balaguer, Jenna |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
120 data points
Data
| 1 Treat | 2 P. antarctica [#/l] (Coulter counter, Beckman Coul...) | 3 POC [mg/l] (1, Element analyser Euro EA 3000) | 4 POC [mg/l] (2, Element analyser Euro EA 3000) | 5 PON [mg/l] (1, Element analyser Euro EA 3000) | 6 PON [mg/l] (2, Element analyser Euro EA 3000) | 7 Exp day [day] |
|---|---|---|---|---|---|---|
| /-FeMn_ Initial_A | 10710000 | 9 | ||||
| /-FeMn_ Initial_B | 13148000 | 9 | ||||
| /-FeMn_ Initial_C | 10534000 | 9 | ||||
| /-FeMn_ A | 105731000 | 0.421826667 | 0.427063333 | 0.079676667 | 0.083023333 | 9 |
| /-FeMn_ B | 115735000 | 0.468576667 | 0.479640000 | 0.092666667 | 0.094333333 | 9 |
| /-FeMn_ C | 108119000 | 0.473853333 | 0.476653333 | 0.093000000 | 0.094626667 | 9 |
| /-Fe_ Initial_A | 13590000 | 9 | ||||
| /-Fe_ Initial_B | 10698000 | 9 | ||||
| /-Fe_ Initial_C | 10537000 | 9 | ||||
| /-Fe_ A | 123618000 | 0.495846667 | 0.490630000 | 0.103630000 | 0.101816667 | 9 |
| /-Fe_ B | 133282000 | 0.535540000 | 0.537220000 | 0.107750000 | 0.107970000 | 9 |
| /-Fe_ C | 119889000 | 0.493296667 | 0.490060000 | 0.098266667 | 0.097340000 | 9 |
| /-Mn_ Initial_A | 4733000 | 11 | ||||
| /-Mn_ Initial_B | 4400000 | 11 | ||||
| /-Mn_ Initial_C | 4346000 | 11 | ||||
| /-Mn_ A | 130016000 | 0.536613333 | 0.531173333 | 0.106783333 | 0.103326667 | 11 |
| /-Mn_ B | 128000000 | 0.577866667 | 0.579623333 | 0.112180000 | 0.112246667 | 11 |
| /-Mn_ C | 140037000 | 0.691046667 | 0.681830000 | 0.132120000 | 0.131550000 | 11 |
| Control_ Initial_A | 3221000 | 10 | ||||
| Control_ Initial_B | 3697000 | 10 | ||||
| Control_ Initial_C | 5527000 | 10 | ||||
| Control_ A | 119372000 | 0.513720000 | 0.511190000 | 0.101746667 | 0.101303333 | 10 |
| Control_ B | 134055000 | 0.528073333 | 0.529436667 | 0.104480000 | 0.103230000 | 10 |
| Control_ C | 127493000 | 0.534033333 | 0.535956667 | 0.104826667 | 0.105636667 | 10 |