Citation:

PANGAEA.944162

Name: Seawater carbonate chemistry and Cu accumulation and cellular toxicity in the Pacific Oyster Crassostrea gigas
Published: 2019
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Behaviour; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Inorganic toxins; Laboratory experiment; Mollusca; North Pacific; Other metabolic rates; Other studied parameter or process; Respiration; Single species; Temperate

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Abstract:

Ocean acidification (OA) has been found to increase the release of free Cu2+ in seawater. However, only a handful of studies have investigated the influence of OA on Cu accumulation and cellular toxicity in bivalve species. In this study, Pacific oysters, Crassostrea gigas, were exposed to 25 μg/L Cu2+ at three pH levels (8.1, 7.8 and 7.6) for 14 and 28 days. Physiological and histopathological parameters [(clearance rate (CR), respiration rate (RR), histopathological damage and condition index (CI)), oxidative stress and neurotoxicity biomarkers [superoxide dismutase (SOD) and glutathione transferase (GST) activities, lipid peroxidation (LPO) and acetylcholinesterase (AChE) activity], combined with glycolytic enzyme activities [pyruvate kinase (PK) and hexokinase (HK)] were investigated in C. gigas. The bioconcentration of Cu was increased in soft tissues of Cu-exposed oysters under OA. Our results suggest that both OA and Cu could lead to physiological disturbance, oxidative stress, cellular damage, disturbance in energy metabolism and neurotoxicity in oysters. The inhibited CR, increased glycolytic enzymes activities and decreased CI suggested that the energy metabolism strategy adopted by oysters was not sustainable in the long term. Furthermore, integrated biomarker response (IBR) results found that OA and Cu exposure lead to severe stress to oysters, and co-exposure was the most stressful condition. Results from this study highlight the need to include OA in future environmental assessments of pollutants and hazardous materials to better elucidate the risks of those environmental perturbations.

Keyword(s):

Animalia; Behaviour; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Inorganic toxins; Laboratory experiment; Mollusca; North Pacific; Other metabolic rates; Other studied parameter or process; Respiration; Single species; Temperate

Supplement to:

Cao, Ruiwen; Zhang, Tianyu; Li, Xiao; Zhao, Y; Wang, Qing; Yang, Dinglong; Qu, Yi; Liu, Hui; Dong, Zhijun; Zhao, Jianmin (2019): Seawater acidification increases copper toxicity: A multi-biomarker approach with a key marine invertebrate, the Pacific Oyster Crassostrea gigas. Aquatic Toxicology, 210, 167-178, https://doi.org/10.1016/j.aquatox.2019.03.002

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-05-13.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Zhao, Jianmin		study
2	Species, unique identification	Species UID		Zhao, Jianmin
3	Species, unique identification (URI)	Species UID (URI)		Zhao, Jianmin
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Zhao, Jianmin
5	Experiment day	Exp day	day	Zhao, Jianmin
6	Treatment	Treat		Zhao, Jianmin
7	Copper	Cu	µg/g	Zhao, Jianmin		accumulation in soft tissues
8	Copper, standard deviation	Cu std dev	±	Zhao, Jianmin		accumulation in soft tissues
9	Replicates	Repl	#	Zhao, Jianmin
10	Clearance rate	CR	ml/g/h	Zhao, Jianmin
11	Clearance rate, standard deviation	CR std dev	±	Zhao, Jianmin
12	Replicates	Repl	#	Zhao, Jianmin
13	Respiration rate, oxygen	Resp O2	mg/g/h	Zhao, Jianmin
14	Respiration rate, oxygen, standard deviation	Resp O2 std dev	±	Zhao, Jianmin
15	Replicates	Repl	#	Zhao, Jianmin
16	Condition index	CI		Zhao, Jianmin
17	Condition index, standard deviation	CI std dev	±	Zhao, Jianmin
18	Replicates	Repl	#	Zhao, Jianmin
19	Acetylcholinesterase activity, unit per protein mass	AChE/prot	U/mg	Zhao, Jianmin
20	Acetylcholinesterase activity, standard deviation	AChE std dev	±	Zhao, Jianmin
21	Replicates	Repl	#	Zhao, Jianmin
22	Superoxide dismutase activity, unit per protein mass	SOD/prot	U/mg	Zhao, Jianmin
23	Superoxide dismutase activity, standard deviation	SOD std dev	±	Zhao, Jianmin
24	Replicates	Repl	#	Zhao, Jianmin
25	Glutathione S-transferase activity, unit per protein mass	GST/prot	U/mg	Zhao, Jianmin
26	Glutathione S-transferase activity, standard deviation	GST std dev	±	Zhao, Jianmin
27	Replicates	Repl	#	Zhao, Jianmin
28	Lipid peroxidation, per protein	LPO/prot	nmol/mg	Zhao, Jianmin
29	Lipid peroxidation, standard deviation	LPO std dev	±	Zhao, Jianmin
30	Replicates	Repl	#	Zhao, Jianmin
31	Pyruvate kinase activity, per protein	PK act	U/g	Zhao, Jianmin
32	Pyruvate kinase activity, standard deviation	PK act std dev	±	Zhao, Jianmin
33	Replicates	Repl	#	Zhao, Jianmin
34	Hexokinase activity, per protein mass	HK act/prot	U/mg	Zhao, Jianmin
35	Hexokinase activity, standard deviation	HK act std dev	±	Zhao, Jianmin
36	Replicates	Repl	#	Zhao, Jianmin
37	Integrated biomarker response index	IBR		Zhao, Jianmin
38	pH	pH		Zhao, Jianmin	Potentiometric	NBS scale
39	pH, standard deviation	pH std dev	±	Zhao, Jianmin	Potentiometric	NBS scale
40	Temperature, water	Temp	°C	Zhao, Jianmin
41	Temperature, water, standard deviation	Temp std dev	±	Zhao, Jianmin
42	Salinity	Sal		Zhao, Jianmin
43	Salinity, standard deviation	Sal std dev	±	Zhao, Jianmin
44	Alkalinity, total	AT	µmol/kg	Zhao, Jianmin	Potentiometric titration
45	Alkalinity, total, standard deviation	AT std dev	±	Zhao, Jianmin	Potentiometric titration
46	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Zhao, Jianmin	Calculated using CO2SYS
47	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Zhao, Jianmin	Calculated using CO2SYS
48	Carbon, inorganic, dissolved	DIC	µmol/kg	Zhao, Jianmin	Calculated using CO2SYS
49	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Zhao, Jianmin	Calculated using CO2SYS
50	Calcite saturation state	Omega Cal		Zhao, Jianmin	Calculated using CO2SYS
51	Calcite saturation state, standard deviation	Omega Cal std dev	±	Zhao, Jianmin	Calculated using CO2SYS
52	Aragonite saturation state	Omega Arg		Zhao, Jianmin	Calculated using CO2SYS
53	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Zhao, Jianmin	Calculated using CO2SYS
54	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
55	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
56	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
57	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
58	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
59	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
60	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
61	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
62	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
63	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

732 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species UID	3 Species UID (URI)	4 Species UID (Semantic URI)	5 Exp day [day]	6 Treat	7 Cu [µg/g] (accumulation in soft tissues)	8 Cu std dev [±] (accumulation in soft tissues)	9 Repl [#]	10 CR [ml/g/h]	11 CR std dev [±]	12 Repl [#]	13 Resp O2 [mg/g/h]	14 Resp O2 std dev [±]	15 Repl [#]	16 CI	17 CI std dev [±]	18 Repl [#]	19 AChE/prot [U/mg]	20 AChE std dev [±]	21 Repl [#]	22 SOD/prot [U/mg]	23 SOD std dev [±]	24 Repl [#]	25 GST/prot [U/mg]	26 GST std dev [±]	27 Repl [#]	28 LPO/prot [nmol/mg]	29 LPO std dev [±]	30 Repl [#]	31 PK act [U/g]	32 PK act std dev [±]	33 Repl [#]	34 HK act/prot [U/mg]	35 HK act std dev [±]	36 Repl [#]	37 IBR	38 pH (NBS scale, Potentiometric)	39 pH std dev [±] (NBS scale, Potentiometric)	40 Temp [°C]	41 Temp std dev [±]	42 Sal	43 Sal std dev [±]	44 AT [µmol/kg] (Potentiometric titration)	45 AT std dev [±] (Potentiometric titration)	46 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	47 pCO2 std dev [±] (Calculated using CO2SYS)	48 DIC [µmol/kg] (Calculated using CO2SYS)	49 DIC std dev [±] (Calculated using CO2SYS)	50 Omega Cal (Calculated using CO2SYS)	51 Omega Cal std dev [±] (Calculated using CO2SYS)	52 Omega Arg (Calculated using CO2SYS)	53 Omega Arg std dev [±] (Calculated using CO2SYS)	54 CSC flag (Calculated using seacarb afte...)	55 pH (total scale, Calculated using...)	56 CO2 [µmol/kg] (Calculated using seacarb afte...)	57 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	58 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	59 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	60 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	61 DIC [µmol/kg] (Calculated using seacarb afte...)	62 Omega Arg (Calculated using seacarb afte...)	63 Omega Cal (Calculated using seacarb afte...)
laboratory	Crassostrea gigas	marinespecies.org	urn:lsid:marinespecies.org:taxname:140656	14	pH=8.1, non-Cu	137.516125	22.89295782	6	1091.6173330	0.431158573	9	0.423941800	0.054260823	18	4.962230600	1.039649117	15	0.858254714	0.118531834	8	35.19094125	3.934212846	8	99.24566750	11.20174146	8	4.502669571	0.840690314	8	138648.4250	10014.687220	8	94.18179333	13.799851810	8	0.000000	8.14	0.02	17.3	0.2	31.2	0.5	2249.12	39.14	444.13	22.21	2055.23	39.41	3.5	0.3	2.3	0.2	26	8.02	15.60	436.51	438.05	1892.88	145.52	2054.0	2.28	3.56
laboratory	Crassostrea gigas	marinespecies.org	urn:lsid:marinespecies.org:taxname:140656	14	pH=7.8, non-Cu	152.935300	28.34542801	6	979.9617667	0.208304263	9	0.309045500	0.062369600	18	4.956822867	1.137086605	15	0.836214250	0.146897611	8	37.45075143	6.600011468	8	107.47142130	27.12785083	8	4.208436750	0.774125388	8	155695.4000	20889.774080	8	107.93830000	15.267355240	8	7.928813	7.77	0.03	17.7	0.5	31.2	0.5	2257.96	47.93	1153.64	85.91	2198.62	53.65	1.7	0.2	1.1	0.1	26	7.65	40.04	1133.70	1137.68	2088.24	69.42	2197.7	1.09	1.70
laboratory	Crassostrea gigas	marinespecies.org	urn:lsid:marinespecies.org:taxname:140656	14	pH=7.6, non-Cu	158.957525	33.89843243	6	1570.6446670	0.288589088	9	0.362493667	0.058825595	18	4.824995067	0.970542002	15	0.812957688	0.101792098	8	36.41924000	5.346070844	8	121.38428250	23.17634224	8	4.793028000	0.805605915	8	191437.8000	27507.077750	8	118.41461000	15.000244290	8	24.068810	7.56	0.02	17.3	0.3	31.2	0.5	2287.56	47.14	1942.54	75.30	2293.53	58.77	1.1	0.1	0.7	0.1	26	7.44	68.29	1911.39	1918.13	2180.03	44.08	2292.4	0.69	1.08
laboratory	Crassostrea gigas	marinespecies.org	urn:lsid:marinespecies.org:taxname:140656	14	pH=8.1, Cu	301.881225	57.05857321	6	1097.2043000	0.311959777	9	0.388335033	0.088858400	18	4.947743933	1.147908981	15	0.948856688	0.151737830	8	41.38864000	5.240030358	8	109.60020860	21.28833137	8	4.578977750	0.763469327	8	161224.9875	8451.238617	8	95.11961625	11.956404650	8	6.205315	8.12	0.04	17.5	0.5	31.2	0.5	2275.13	33.26	476.06	61.10	2087.13	19.32	3.5	0.5	2.2	0.3	26	8.00	16.56	466.30	467.93	1927.16	142.47	2086.2	2.24	3.49
laboratory	Crassostrea gigas	marinespecies.org	urn:lsid:marinespecies.org:taxname:140656	14	pH=7.8, Cu	287.766250	65.57531789	6	1568.5183330	0.303648563	9	0.407733900	0.115760812	18	5.017399200	0.802298329	15	0.876975000	0.086194233	8	38.61189750	6.871365018	8	89.57008500	14.21569540	8	4.829812143	1.212891077	8	180734.2500	17381.534960	8	97.86547750	11.445652500	8	16.880450	7.79	0.02	17.5	0.6	31.2	0.5	2263.95	33.53	1124.15	90.23	2248.47	32.06	1.8	0.1	1.1	0.1	26	7.67	38.40	1080.92	1084.72	2087.59	72.12	2198.1	1.13	1.77
laboratory	Crassostrea gigas	marinespecies.org	urn:lsid:marinespecies.org:taxname:140656	14	pH=7.6, Cu	397.857475	161.59942370	6	831.7329333	0.186124670	9	0.355460933	0.051710137	18	3.526267333	0.555784907	15	0.704797063	0.140629296	8	46.09459500	7.857466448	8	116.34427500	73.70166669	8	6.139121500	1.144764661	8	181462.0750	24477.753850	8	106.83738860	10.360054960	8	29.033590	7.59	0.02	17.7	0.5	31.2	0.5	2273.83	41.49	1802.78	76.37	2269.29	50.56	1.2	0.1	0.7	0.1	26	7.47	62.68	1775.02	1781.25	2158.16	47.36	2268.2	0.74	1.16
laboratory	Crassostrea gigas	marinespecies.org	urn:lsid:marinespecies.org:taxname:140656	28	pH=8.1, non-Cu	160.602450	20.48255873	6	873.0037000	0.287329469	9	0.430483900	0.038867741	18	5.520648867	1.020294856	15	0.902314150	0.243537742	8	35.33405375	4.044088849	8	93.04257125	55.93587127	8	3.616707333	1.224307288	8	133452.3000	13148.682470	8	110.40912250	18.078272610	8	0.000000	8.14	0.02	17.3	0.2	31.2	0.5	2249.12	39.14	444.13	22.21	2055.23	39.41	3.5	0.3	2.3	0.2	26	8.02	15.60	436.51	438.05	1892.88	145.52	2054.0	2.28	3.56
laboratory	Crassostrea gigas	marinespecies.org	urn:lsid:marinespecies.org:taxname:140656	28	pH=7.8, non-Cu	192.079175	47.61592612	6	852.4206333	0.110093889	9	0.408746033	0.089398109	18	5.566415200	0.486393833	15	0.961102813	0.115718428	8	42.33155250	2.973821822	8	139.02934290	18.03089324	8	3.538356286	1.111381214	8	140907.7750	20307.009030	8	122.98541630	20.530378390	8	5.608838	7.77	0.03	17.7	0.5	31.2	0.5	2257.96	47.93	1153.64	85.91	2198.62	53.65	1.7	0.2	1.1	0.1	26	7.65	40.04	1133.70	1137.68	2088.24	69.42	2197.7	1.09	1.70
laboratory	Crassostrea gigas	marinespecies.org	urn:lsid:marinespecies.org:taxname:140656	28	pH=7.6, non-Cu	188.787625	51.36307413	6	890.3342000	0.215894016	9	0.477650167	0.034965553	18	5.306029333	0.588035228	15	0.651983125	0.141189912	8	42.15931000	3.548105882	8	109.98846130	38.08662672	8	4.120210857	1.378737196	8	133170.3000	21319.367700	8	108.45322430	13.815667480	8	17.744400	7.56	0.02	17.3	0.3	31.2	0.5	2287.56	47.14	1942.54	75.30	2293.53	58.77	1.1	0.1	0.7	0.1	26	7.44	68.29	1911.39	1918.13	2180.03	44.08	2292.4	0.69	1.08
laboratory	Crassostrea gigas	marinespecies.org	urn:lsid:marinespecies.org:taxname:140656	28	pH=8.1, Cu	418.325025	68.16059228	6	449.2871333	0.240913724	9	0.326559400	0.031229851	18	5.539781733	0.719930881	15	1.155394063	0.192387174	8	38.36085875	3.764646367	8	121.59811750	19.45340597	8	4.087047667	1.641723933	8	133295.4843	26550.423880	8	112.20612400	21.873178780	8	6.409133	8.12	0.04	17.5	0.5	31.2	0.5	2275.13	33.26	476.06	61.10	2087.13	19.32	3.5	0.5	2.2	0.3	26	8.00	16.56	466.30	467.93	1927.16	142.47	2086.2	2.24	3.49
laboratory	Crassostrea gigas	marinespecies.org	urn:lsid:marinespecies.org:taxname:140656	28	pH=7.8, Cu	454.553500	128.06741720	6	722.8493000	0.221454675	9	0.338103267	0.097582722	18	5.405896667	0.706219131	15	0.900793786	0.171353652	8	41.69787714	4.941895319	8	173.26355710	43.45178018	8	4.711455625	1.173936363	8	134466.0125	13184.049120	8	123.20818750	8.072814975	8	23.522620	7.79	0.02	17.5	0.6	31.2	0.5	2263.95	33.53	1124.15	90.23	2248.47	32.06	1.8	0.1	1.1	0.1	26	7.67	38.40	1080.92	1084.72	2087.59	72.12	2198.1	1.13	1.77
laboratory	Crassostrea gigas	marinespecies.org	urn:lsid:marinespecies.org:taxname:140656	28	pH=7.6, Cu	603.380975	50.27137994	6	176.9431400	0.111686697	9	0.355532267	0.063416796	18	4.491895467	1.074937666	15	0.948872313	0.170330052	8	45.24027500	4.805731151	8	188.42307500	51.86933542	8	6.173284000	1.176172162	8	147697.6375	19416.033320	8	139.50686670	22.954943880	8	33.199430	7.59	0.02	17.7	0.5	31.2	0.5	2273.83	41.49	1802.78	76.37	2269.29	50.56	1.2	0.1	0.7	0.1	26	7.47	62.68	1775.02	1781.25	2158.16	47.36	2268.2	0.74	1.16