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Bode, Antonio; Saavedra, Camilio; Álvarez-González, Miguel; Arregui, Marina; Arbelo, Manuel; Fernández, A; Freitas, Luís; Silva, Mónica A; Prieto, Rui; Azevedo, José M N; Giménez, Joan; Pierce, Graham J; Santos, M Begoña (2022): Stable C and N isotope data for cetacea collected in the Macaronesian region between 1996 and 2018 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.943603

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Abstract:
This dataset provides the values of stable carbon and nitrogen stable isotopes in bulk muscle samples of 11 species of cetacea from the Macaronesian regions (Canary, Madeira, and Azores Islands) collected between 1996 and 2018. The values of nitrogen stable isotopes in amino acids of muscle samples of the common dolphin (Delphinus delphis) were also provided. The samples were collected from stranded animals by trained personnel. Cetacean samples were obtained from necropsies of stranded cetaceans following a standard protocol defined by the European Cetacean Society (after Kuiken and García Hartmann 1991). Additional data included body length, age (adult, juvenile) and sex for each animal, along with carbon, nitrogen, and lipid content of muscle samples. Isotope data for bulk samples included values for samples with and without lipids. Exact latitude and longitude coordinates for each sample are not available, geographical position of the center of a circle including each island is given instead. Samples were freeze dried or dried (60°C, 48h) before analysis. Stable isotopes in bulk muscle samples were analysed in an isotope-ratio mass spectrometer coupled to an elemental analyser. Aliquots of each sample were analysed whole or after lipid extraction with trichloromethane:methanol (Bligh and Dyer, 1959). Stable nitrogen isotopes in amino acids were analysed after hydrolisis and derivatization of samples in an isotope-ratio mass spectrometer coupled to a gas chromatograph. Details on the analytical procedures can be found in Bode et al. (2021).
Keyword(s):
Atlantic spotted dolphin; bottlenose dolphin; Bryde's whale; Carbon isotopes; common dolphin; Cuvier's beaked whale; fin whale; nitrogen isotopes; pygmy sperm whale; Risso's dolphin; short-finned pilot whale; sperm whale; Stable isotopes; striped dolphin
Supplement to:
Bode, Antonio; Saavedra, Camilio; Álvarez-González, Miguel; Arregui, Marina; Arbelo, Manuel; Fernández, Antonio; Freitas, Luís; Silva, Mónica A; Prieto, Rui; Azevedo, José M N; Giménez, Joan; Pierce, Graham J; Santos, M Begoña (2022): Trophic position of dolphins tracks recent changes in the pelagic ecosystem of the Macaronesian region (NE Atlantic). Marine Ecology Progress Series, 699, 167-180, https://doi.org/10.3354/meps14176
Further details:
Bligh, E G; Dyer, W J (1959): A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37(8), 911-917, https://doi.org/10.1139/o59-099
Bode, Antonio; Olivar, M Pilar; López-Pérez, Cristina; Hernández-León, Santiago (2021): The microbial contribution to the trophic position of stomiiform fishes. ICES Journal of Marine Science, fsab189, https://doi.org/10.1093/icesjms/fsab189
Kuiken, Thijs; García Hartmann, Manuel (1991): Cetacean pathology: dissection techniques and tissue sampling. European Cetacean Society Newsletter, Special Issue, 17, 1-39, KuikenGarciaHartmann1991ECS.pdf
Funding:
Agencia Estatal de Investigación (AEI), grant/award no. PID2020-115620RB-100: QLOCKS project
European Commission (EC), grant/award no. 11.0661/2017/750679/SUB/ENV.C2: MISCTIC SEAS2: Seguimiento y evaluación coordinada de la biodiversidad marina en la Macaronesia
European Commission (EC), grant/award no. ACORES-01-0145-FEDER-000140: InvestigadoresMarAZ-Consolidar corpo de investigadores em Ciências do Mar nos Açores
European Commission (EC), grant/award no. MAC/1.1b/149: MARCET- Red Macaronésica de Transferencia de Conocimientos y Tecnologías Interregional y Multidisciplinar para proteger, vigilar y monitorizar los cetáceos y el medio marino, y analizar y explotar de forma sostenible la actividad Turística asociada
European Commission (EC), grant/award no. MAC2/4.6c/392: MARCET II - Promotion of whale watching ecotourism activity as a model of sustainable economic development by the protection and conservation of cetacean populations and increasing their value as a natural heritage of the Macaronesia
Fundação para a Ciência e Tecnologia (FCT), grant/award no. IF/00943/2013/ CP1199/CT0001: MOVE-ON: Movement ecology of whales: investigating environmental, social and physiological motivations
Fundação para a Ciência e Tecnologia (FCT), grant/award no. M2.1.2/F/012/2011: MAPCET - Integrating cetaceans into marine spacial management in the Azores
Fundação para a Ciência e Tecnologia (FCT), grant/award no. PTDC/MAR/74071/2006: TRACE - Cetacean habitat associations in oceanic systems: an integrated approach
Fundação para a Ciência e Tecnologia (FCT), grant/award no. UIDB/05634/2020
Horizon 2020 (H2020), grant/award no. 817806: Sustainable management of mesopelagic resources
Ministerio de Ciencia e Innovación (MICINN), grant/award no. CEX2019-000928-S: Severo Ochoa Centre of Excellence / Severo Ochoa Excellence Award
Ministerio de Ciencia e Innovación (MICINN), grant/award no. FJC2019-040016-I
Ministerio de Economía y Competitividad (MEC), grant/award no. Commission [28-5307]: Technical Scientific Advice for the Protection of the Marine Environment: Assessment and Monitoring of Marine Strategies, Monitoring of Marine Protected Areas of State Competence (2018-2021)
Regional Secretariat for the Sea, Science and Technology, Government of Azores, grant/award no. M1.1.A/REEQ.CIENTÍFICO UI&D/2021/010: Reequipamento científico OKEANOS
Coverage:
Median Latitude: 32.245462 * Median Longitude: -19.335077 * South-bound Latitude: 27.971000 * West-bound Longitude: -28.707000 * North-bound Latitude: 38.748000 * East-bound Longitude: -13.514000
Date/Time Start: 2000-02-29T00:00:00 * Date/Time End: 2018-08-03T00:00:00
Minimum DEPTH, water: m * Maximum DEPTH, water: m
Event(s):
Cetacea_Faial * Latitude: 38.583000 * Longitude: -28.707000 * Date/Time Start: 2014-02-23T00:00:00 * Date/Time End: 2017-06-26T00:00:00 * Location: Faial, Azores Islands, Portugal * Method/Device: Necropsy after Kuiken and García Hartmann (1991)
Cetacea_Fuerteventura * Latitude: 28.362000 * Longitude: -14.106000 * Date/Time Start: 2001-11-26T00:00:00 * Date/Time End: 2018-01-22T00:00:00 * Location: Fuerteventura, Canary Islands, Spain * Method/Device: Necropsy after Kuiken and García Hartmann (1991)
Cetacea_Gran_Canaria * Latitude: 27.971000 * Longitude: -15.566000 * Date/Time Start: 2000-03-16T00:00:00 * Date/Time End: 2015-02-17T00:00:00 * Location: Gran Canaria, Canary Islands, Spain * Method/Device: Necropsy after Kuiken and García Hartmann (1991)
Comment:
This research was supported by the Madeira Cetacean Stranding Network (RACAM) of the Machico Municipality, Madeira, Portugal.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventBode, Antonio
2Sample IDSample IDBode, Antonio
3SpeciesSpeciesBode, Antonio
4Species codeSpec codeBode, Antonio
5Uniform resource locator/link to referenceURL refBode, AntonioWoRMS AphiaID
6Area/localityAreaBode, Antonio
7LocationLocationBode, Antonio
8Year of observationYear obsa ADBode, Antonio
9DATE/TIMEDate/TimeBode, AntonioGeocode – sampling
10Latitude of eventLatitudeBode, Antonio
11Longitude of eventLongitudeBode, Antonio
12DEPTH, waterDepth watermBode, AntonioGeocode
13SexSexBode, AntonioVisual observation
14Length, totalTLmmBode, AntonioTape measuretotal length
15Life stageLife stageBode, AntonioVisual observation
16LipidsLipids%Bode, AntonioGravimetry (GRAV)of dry mass
17Nitrogen, totalTN%Bode, AntonioElemental analyser - isotope ratio mass spectrometryof dry mass in untreated samples
18δ15Nδ15N‰ airBode, AntonioElemental analyser - isotope ratio mass spectrometryuntreated samples
19Carbon, totalTC%Bode, AntonioElemental analyser - isotope ratio mass spectrometryof dry mass in untreated samples
20δ13Cδ13C‰ PDBBode, AntonioElemental analyser - isotope ratio mass spectrometryuntreated samples
21Carbon/Nitrogen ratioC/NBode, AntonioElemental analyser - isotope ratio mass spectrometryuntreated samples
22Nitrogen, totalTN%Bode, AntonioElemental analyser - isotope ratio mass spectrometryof dry mass in delipidized samples
23δ15Nδ15N‰ airBode, AntonioElemental analyser - isotope ratio mass spectrometrydelipidized samples
24Carbon, totalTC%Bode, AntonioElemental analyser - isotope ratio mass spectrometryof dry mass in delipidized samples
25δ13Cδ13C‰ PDBBode, AntonioElemental analyser - isotope ratio mass spectrometrydelipidized samples
26Carbon/Nitrogen ratioC/NBode, AntonioCalculateddelipidized samples
27Alanine, δ15NAla δ15N‰ airBode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
28Glycine, δ15NGly δ15N‰ airBode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
29Threonine, δ15NThr δ15N‰ airBode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
30Serine, δ15NSer δ15N‰ airBode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
31Valine, δ15NVal δ15N‰ airBode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
32Leucine, δ15NLeu δ15N‰ airBode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
33Isoleucine, δ15NIle δ15N‰ airBode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
34Proline, δ15NPro δ15N‰ airBode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
35Aspartamine and Aspartic acid, δ15NAsn + Asp δ15N‰ airBode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
36Methionine, δ15NMet δ15N‰ airBode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
37Glutamine and Glutamic acid, δ15NGln + Glu δ15N‰ airBode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
38Phenylalanine, δ15NPhe δ15N‰ airBode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
39Lysine, δ15NLys δ15N‰ airBode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
40AlanineAla%Bode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
41GlycineGly%Bode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
42ThreonineThr%Bode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
43SerineSer%Bode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
44ValineVal%Bode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
45LeucineLeu%Bode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
46IsoleucineIle%Bode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
47ProlinePro%Bode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
48Aspartamine and Aspartic acidAsn + Asp%Bode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
49MethionineMet%Bode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
50Glutamine and Glutamic acidGln + Glu%Bode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
51PhenylalaninePhe%Bode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
52LysineLys%Bode, AntonioGas chromatography - Isotope ratio mass spectrometer (GC-IRMS)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2379 data points

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