Citation:

PANGAEA.943567

Name: Seawater carbonate chemistry and carbonate chemistry in the microenvironment within cyanobacterial aggregates
Published: 2021
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Acid-base regulation; Bacteria; Baltic Sea; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Cyanobacteria; Dolichospermum sp.; Heterotrophic prokaryotes; Laboratory experiment; Nodularia spumigena; Pelagos; Respiration; Single species; Temperate

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook TwitterShow Map Google Earth

Abstract:

Photosynthesis and respiration cause distinct chemical microenvironments within cyanobacterial aggregates. Here, we used microsensors and a diffusion–reaction model to characterize gradients in carbonate chemistry and investigate how these are affected by ocean acidification in Baltic vs. Pacific aggregates (Nodularia and Dolichospermum vs. Trichodesmium). Microsensor measurements of O2 and pH were performed under in situ and expected future pCO2 levels on Nodularia and Dolichospermum aggregates collected in the Baltic Sea. Under in situ conditions, O2 and pH levels within the aggregates covered ranges of 80–175% air saturation and 7.7–9.4 in dark and light, respectively. Carbon uptake in the light was predicted to reduce HCO3− by 100–150 μmol/L and CO2 by 3–6 μmol/L in the aggregate center compared to outside, inducing strong CO2 depletion (down to 0.5 μmol/L CO2 remaining in the center) even when assuming that HCO3− covered 80–90% of carbon uptake. Under ocean acidification conditions, enhanced CO2 availability allowed for significantly lower activity of carbon concentrating mechanisms, including a reduction of the contribution of HCO3− to carbon uptake by up to a factor of 10. The magnification of proton gradients under elevated pCO2 that was predicted based on a lower buffer capacity was observed in measurements despite a concurrent decrease in photosynthetic activity. In summary, we provide a quantitative image of the inorganic carbon environment in cyanobacterial aggregates under present-day and expected future conditions, considering both the individual and combined effects of the chemical and biological processes that shape these environments.

Keyword(s):

Acid-base regulation; Bacteria; Baltic Sea; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Cyanobacteria; Dolichospermum sp.; Heterotrophic prokaryotes; Laboratory experiment; Nodularia spumigena; Pelagos; Respiration; Single species; Temperate

Supplement to:

Eichner, Meri; Wolf-Gladrow, Dieter A; Ploug, Helle (2022): Carbonate chemistry in the microenvironment within cyanobacterial aggregates under present‐day and future pCO2 levels. Limnology and Oceanography, 67(1), 203-218, https://doi.org/10.1002/lno.11986

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 58.832700 * Longitude: 17.757500

Event(s):

station_B1 * Latitude: 58.832700 * Longitude: 17.757500 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-04-28.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Eichner, Meri		study
2	Species	Species		Eichner, Meri
3	Registration number of species	Reg spec no		Eichner, Meri		WoRMS Aphia ID
4	Uniform resource locator/link to reference	URL ref		Eichner, Meri
5	Identification	ID		Eichner, Meri
6	Treatment	Treat		Eichner, Meri		Bulk pH
7	Light mode	L mode		Eichner, Meri
8	pH	pH		Eichner, Meri		in center
9	pH, standard deviation	pH std dev	±	Eichner, Meri		in center
10	Hydrogen ion concentration	H+	nmol/l	Eichner, Meri		in center
11	Hydrogen ion concentration, standard deviation	H+ std dev	±	Eichner, Meri		in center
12	Ratio	Ratio		Eichner, Meri		between d[H+]
13	Oxygen	O2	µmol/l	Eichner, Meri		in center
14	Oxygen, standard deviation	O2 std dev	±	Eichner, Meri		in center
15	Oxygen evolution per individual	O2 ev/ind	nmol/h/#	Eichner, Meri		per aggregates
16	Oxygen evolution, standard deviation	O2 ev std dev	±	Eichner, Meri		per aggregates
17	Ratio	Ratio		Eichner, Meri		between O2 evolution or uptake rates
18	Oxygen evolution	O2 ev	nmol/h/mm³	Eichner, Meri
19	Oxygen evolution, standard deviation	O2 ev std dev	±	Eichner, Meri
20	Salinity	Sal		Eichner, Meri
21	Temperature, water	Temp	°C	Eichner, Meri
22	pH	pH		Eichner, Meri	Potentiometric	NBS scale
23	pH, standard deviation	pH std dev	±	Eichner, Meri	Potentiometric	NBS scale
24	Alkalinity, total	AT	µmol/kg	Eichner, Meri	Potentiometric titration
25	Alkalinity, total, standard deviation	AT std dev	±	Eichner, Meri	Potentiometric titration
26	Carbon, inorganic, dissolved	DIC	µmol/kg	Eichner, Meri	Colorimetric
27	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Eichner, Meri	Colorimetric
28	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Eichner, Meri	Calculated using CO2SYS
29	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
31	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1101 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no (WoRMS Aphia ID)	4 URL ref	5 ID	6 Treat (Bulk pH)	7 L mode	8 pH (in center)	9 pH std dev [±] (in center)	10 H+ [nmol/l] (in center)	11 H+ std dev [±] (in center)	12 Ratio (between d[H+])	13 O2 [µmol/l] (in center)	14 O2 std dev [±] (in center)	15 O2 ev/ind [nmol/h/#] (per aggregates)	16 O2 ev std dev [±] (per aggregates)	17 Ratio (between O2 evolution or uptak...)	18 O2 ev [nmol/h/mm³]	19 O2 ev std dev [±]	20 Sal	21 Temp [°C]	22 pH (NBS scale, Potentiometric)	23 pH std dev [±] (NBS scale, Potentiometric)	24 AT [µmol/kg] (Potentiometric titration)	25 AT std dev [±] (Potentiometric titration)	26 DIC [µmol/kg] (Colorimetric)	27 DIC std dev [±] (Colorimetric)	28 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	29 CSC flag (Calculated using seacarb afte...)	30 pH (total scale, Calculated using...)	31 CO2 [µmol/kg] (Calculated using seacarb afte...)	32 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	33 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	34 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	35 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	36 DIC [µmol/kg] (Calculated using seacarb afte...)	37 Omega Arg (Calculated using seacarb afte...)	38 Omega Cal (Calculated using seacarb afte...)
laboratory	Dolichospermum sp. (prokaryotes)			D1	8.2	Light	8.8		12			402		1.7			4.6		6	15	8.33	0.15	1542	11	1401	29	272	26	8.18	12.64	286.93	287.97	1400.02	65.54	1478.20	1.05	1.89
laboratory	Dolichospermum sp. (prokaryotes)			D1	8.2	Dark	7.9					322		0.6			3.5		6	15	8.33	0.15	1542	11	1401	29	272	26	8.18	12.64	286.93	287.97	1400.02	65.54	1478.20	1.05	1.89
laboratory	Dolichospermum sp. (prokaryotes)			D2	8.3	Light	8.7		6			347		1.0			2.6		6	15	8.33	0.15	1542	11	1401	29	272	26	8.18	12.64	286.93	287.97	1400.02	65.54	1478.20	1.05	1.89
laboratory	Dolichospermum sp. (prokaryotes)			D2	8.3	Dark	8.1					289		0.6			2.3		6	15	8.33	0.15	1542	11	1401	29	272	26	8.18	12.64	286.93	287.97	1400.02	65.54	1478.20	1.05	1.89
laboratory	Dolichospermum sp. (prokaryotes)			D3	8.3	Light	9.0		11			427		4.5			7.1		6	15	8.33	0.15	1542	11	1401	29	272	26	8.18	12.64	286.93	287.97	1400.02	65.54	1478.20	1.05	1.89
laboratory	Dolichospermum sp. (prokaryotes)			D3	8.3	Dark	7.9					290		1.3			1.5		6	15	8.33	0.15	1542	11	1401	29	272	26	8.18	12.64	286.93	287.97	1400.02	65.54	1478.20	1.05	1.89
laboratory	Nodularia spumigena (prokaryotes)	160566	marinespecies.org	N1	8.2	Light	9.0		18			375		0.7			21.0		6	15	8.33	0.15	1542	11	1401	29	272	26	8.18	12.64	286.93	287.97	1400.02	65.54	1478.20	1.05	1.89
laboratory	Nodularia spumigena (prokaryotes)	160566	marinespecies.org	N1	8.2	Dark	7.7					264		1.3			6.3		6	15	8.33	0.15	1542	11	1401	29	272	26	8.18	12.64	286.93	287.97	1400.02	65.54	1478.20	1.05	1.89
laboratory	Nodularia spumigena (prokaryotes)	160566	marinespecies.org	N2	8.6	Light	9.3		3			455		4.0			10.5		6	15	8.33	0.15	1542	11	1401	29	272	26	8.18	12.64	286.93	287.97	1400.02	65.54	1478.20	1.05	1.89
laboratory	Nodularia spumigena (prokaryotes)	160566	marinespecies.org	N2	8.6	Dark	8.4					242		2.3			2.7		6	15	8.33	0.15	1542	11	1401	29	272	26	8.18	12.64	286.93	287.97	1400.02	65.54	1478.20	1.05	1.89
laboratory	Nodularia spumigena (prokaryotes)	160566	marinespecies.org	N3	8.5	Light	9.4		15			489		10.1			7.7		6	15	8.33	0.15	1542	11	1401	29	272	26	8.18	12.64	286.93	287.97	1400.02	65.54	1478.20	1.05	1.89
laboratory	Nodularia spumigena (prokaryotes)	160566	marinespecies.org	N3	8.5	Dark	7.8					239		4.6			1.3		6	15	8.33	0.15	1542	11	1401	29	272	26	8.18	12.64	286.93	287.97	1400.02	65.54	1478.20	1.05	1.89
laboratory	Nodularia spumigena (prokaryotes)	160566	marinespecies.org	N4	8.6	Light	9.4					532		11.9			9.2		6	15	8.33	0.15	1542	11	1401	29	272	26	8.18	12.64	286.93	287.97	1400.02	65.54	1478.20	1.05	1.89
laboratory	Nodularia spumigena (prokaryotes)	160566	marinespecies.org	N4	8.6	Dark													6	15	8.33	0.15	1542	11	1401	29	272	26	8.18	12.64	286.93	287.97	1400.02	65.54	1478.20	1.05	1.89
laboratory	Dolichospermum sp. (prokaryotes)			D1	7.9	Light	8.2		9		0.8	345		0.3		0.2	3.0		6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Dolichospermum sp. (prokaryotes)			D1	7.9	Dark	7.8					305		0.5		0.8	3.9		6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Dolichospermum sp. (prokaryotes)			D2	7.8	Light	8.0		13		2.3	357		0.8		0.9	1.8		6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Dolichospermum sp. (prokaryotes)			D2	7.8	Dark	7.6					308		0.7		1.1	1.3		6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Dolichospermum sp. (prokaryotes)			D3	7.9	Light	8.4		23		2.1	382		1.8		0.4	6.5		6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Dolichospermum sp. (prokaryotes)			D3	7.9	Dark	7.6					291		0.9		0.7	2.1		6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Dolichospermum sp. (prokaryotes)			D4	7.8	Light	8.2												6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Dolichospermum sp. (prokaryotes)			D4	7.8	Dark													6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Dolichospermum sp. (prokaryotes)			D5	7.8	Light	8.2		18										6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Dolichospermum sp. (prokaryotes)			D5	7.8	Dark	7.6												6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Dolichospermum sp. (prokaryotes)			D6	7.7	Light	8.5		15										6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Dolichospermum sp. (prokaryotes)			D6	7.7	Dark	7.7												6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Dolichospermum sp. (prokaryotes)			D7	7.8	Light	9.2		49		1.9	615		32.0		1.1	15.6		6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Dolichospermum sp. (prokaryotes)			D7	7.8	Dark	7.3					167		9.2		1.3	5.0		6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Nodularia spumigena (prokaryotes)	160566	marinespecies.org	N1	7.8	Light	8.2		25		1.4	375		0.9		1.3	14.5		6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Nodularia spumigena (prokaryotes)	160566	marinespecies.org	N1	7.8	Dark													6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Nodularia spumigena (prokaryotes)	160566	marinespecies.org	N4	7.9	Light	9.0					468		6.6		0.6	9.0		6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Nodularia spumigena (prokaryotes)	160566	marinespecies.org	N4	7.9	Dark													6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Nodularia spumigena (prokaryotes)	160566	marinespecies.org	N5	7.9	Light	8.5		26		5.0	417		4.5		2.4	4.8		6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory	Nodularia spumigena (prokaryotes)	160566	marinespecies.org	N5	7.9	Dark	7.5												6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory					A-CO2	Light	8.9	0.2	31	24		446	69	7.0	4		7.0	5	6	15	8.33	0.15	1542	11	1401	29	272	26	8.18	12.64	286.93	287.97	1400.02	65.54	1478.20	1.05	1.89
laboratory					A-CO2	Dark	7.6	0.3				227	59	5.0	3		3.0	2	6	15	8.33	0.15	1542	11	1401	29	272	26	8.18	12.64	286.93	287.97	1400.02	65.54	1478.20	1.05	1.89
laboratory					F-CO2	Light	8.5	0.5	33	21		418	75	5.0	2		9.0	8	6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81
laboratory					F-CO2	Dark	7.4	0.2				263	55	4.0	2		3.0	1	6	15	7.94	0.20	1538	12	1466	22	707	26	7.79	32.67	741.58	744.27	1476.81	28.22	1537.70	0.45	0.81