Xie, Emei; Xu, Kai; Li, Zhengke; Li, Wei; Yi, Xiangqi; Li, Hongzhou; Han, Yonghe; Zhang, Hong; Zhang, Yong (2021): Seawater carbonate chemistry and elemental contents and macromolecules of the coccolithophore Emiliania huxleyi [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.943450
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Abstract:
Elemental contents change with shifts in macromolecular composition of marine phytoplankton. Recent studies focus on the responses of elemental contents of coccolithophores, a major calcifying phytoplankton group, to changing carbonate chemistry, caused by the dissolution of anthropogenically derived CO2 into the surface ocean. However, the effects of changing carbonate chemistry on biomacromolecules, such as protein and carbohydrate of coccolithophores, are less documented. Here, we disentangled the effects of elevated dissolved inorganic carbon (DIC) concentration (900 to 4,930μmol/kg) and reduced pH value (8.04 to 7.70) on physiological rates, elemental contents, and macromolecules of the coccolithophore Emiliania huxleyi. Compared to present DIC concentration and pH value, combinations of high DIC concentration and low pH value (ocean acidification) significantly increased pigments content, particulate organic carbon (POC), and carbohydrate content and had less impact on growth rate, maximal relative electron transport rate (rETRmax), particulate organic nitrogen (PON), and protein content. In high pH treatments, elevated DIC concentration significantly increased growth rate, pigments content, rETRmax, POC, particulate inorganic carbon (PIC), protein, and carbohydrate contents. In low pH treatments, the extents of the increase in growth rate, pigments and carbohydrate content were reduced. Compared to high pH value, under low DIC concentration, low pH value significantly increased POC and PON contents and showed less impact on protein and carbohydrate contents; however, under high DIC concentration, low pH value significantly reduced POC, PON, protein, and carbohydrate contents. These results showed that reduced pH counteracted the positive effects of elevated DIC concentration on growth rate, rETRmax, POC, PON, carbohydrate, and protein contents. Elevated DIC concentration and reduced pH acted synergistically to increase the contribution of carbohydrate–carbon to POC, and antagonistically to affect the contribution of protein–nitrogen to PON, which further shifted the carbon/nitrogen ratio of E. huxleyi.
Keyword(s):
Supplement to:
Xie, Emei; Xu, Kai; Li, Zhengke; Li, Wei; Yi, Xiangqi; Li, Hongzhou; Han, Yonghe; Zhang, Hong; Zhang, Yong (2021): Disentangling the Effects of Ocean Carbonation and Acidification on Elemental Contents and Macromolecules of the Coccolithophore Emiliania huxleyi. Frontiers in Microbiology, 12, https://doi.org/10.3389/fmicb.2021.737454
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-04-22.
Parameter(s):
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
284 data points
Data
1 Type (study) | 2 Species | 3 Reg spec no (WoRMS Aphia ID) | 4 URL ref | 5 Exp duration [days] | 6 Treat | 7 µ [1/day] | 8 µ std dev [±] | 9 Chl a/cell [pg/#] | 10 Chl a std dev [±] | 11 Carotenoids/cell [pg/#] | 12 Carotenoids std dev [±] | 13 Carotenoids/Chl a | 14 Carotenoids/Chl a std dev [±] | 15 rETR max | 16 rETR max std dev [±] | 17 alpha | 18 alpha std dev [±] | 19 Ik [µmol/m2/s] | 20 Ik std dev [±] | 21 PIC/cell [pg/#] | 22 PIC std dev [±] | 23 POC/cell [pg/#] | 24 POC std dev [±] | 25 PON/cell [pg/#] | 26 PON std dev [±] | 27 PIC/POC | 28 PIC/POC ratio std dev [±] | 29 POC/PON | 30 POC/PON std dev [±] | 31 Protein/cell [pg/#] | 32 Protein std dev [±] | 33 CHO/cell [pg/#] | 34 CHO tot std dev [±] | 35 POC [%] (Allocated to protein) | 36 POC std dev [±] (Allocated to protein) | 37 POC [%] (Allocated to carbohydrate) | 38 POC std dev [±] (Allocated to carbohydrate) | 39 POC [%] (Allocated to Chl a) | 40 POC std dev [±] (Allocated to Chl a) | 41 POC [%] (Allocated to carotenoid) | 42 POC std dev [±] (Allocated to carotenoid) | 43 PON [%] (Allocated to protein) | 44 PON std dev [±] (Allocated to protein) | 45 PON [%] (Allocated to Chl a) | 46 PON std dev [±] (Allocated to Chl a) | 47 Sal | 48 Temp [°C] | 49 pCO2water_SST_wet [µatm] (Calculated using CO2SYS) | 50 pCO2 std dev [±] (Calculated using CO2SYS) | 51 pH (total scale, Potentiometric) | 52 pH std dev [±] (total scale, Potentiometric) | 53 AT [µmol/kg] (Potentiometric titration) | 54 AT std dev [±] (Potentiometric titration) | 55 DIC [µmol/kg] (Calculated using CO2SYS) | 56 DIC std dev [±] (Calculated using CO2SYS) | 57 [HCO3]- [µmol/kg] (Calculated using CO2SYS) | 58 [HCO3]- std dev [±] (Calculated using CO2SYS) | 59 [CO3]2- [µmol/kg] (Calculated using CO2SYS) | 60 [CO3]2- std dev [±] (Calculated using CO2SYS) | 61 CO2 [µmol/kg] (Calculated using CO2SYS) | 62 CO2 std dev [±] (Calculated using CO2SYS) | 63 CSC flag (Calculated using seacarb afte...) | 64 CO2 [µmol/kg] (Calculated using seacarb afte...) | 65 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 66 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 67 [HCO3]- [µmol/kg] (Calculated using seacarb afte...) | 68 [CO3]2- [µmol/kg] (Calculated using seacarb afte...) | 69 DIC [µmol/kg] (Calculated using seacarb afte...) | 70 Omega Arg (Calculated using seacarb afte...) | 71 Omega Cal (Calculated using seacarb afte...) |
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laboratory | Emiliania huxleyi (phytoplankton) | 115104 | marinespecies.org | 2 | Present DIC and pH | 1.33 | 0.01 | 0.11 | 0.01 | 0.08 | 0.02 | 0.73 | 0.03 | 252.14 | 10.84 | 0.58 | 0.030 | 433.78 | 13.68 | 4.17 | 0.29 | 10.06 | 0.29 | 1.68 | 0.06 | 0.42 | 0.04 | 6.00 | 0.36 | 5.76 | 0.23 | 3.34 | 0.18 | 30.39 | 1.88 | 13.28 | 0.87 | 0.78 | 0.05 | 0.61 | 0.02 | 54.93 | 1.60 | 0.40 | 0.01 | 33 | 20 | 322 | 6 | 8.11 | 0.01 | 2133 | 10 | 1854 | 10 | 1647 | 11 | 196 | 2 | 10.5 | 0.2 | 8 | 10.29 | 314.13 | 315.20 | 1673.60 | 184.82 | 1868.71 | 2.90 | 4.49 |
laboratory | Emiliania huxleyi (phytoplankton) | 115104 | marinespecies.org | 2 | Reduced pH | 1.16 | 0.02 | 0.07 | 0.01 | 0.06 | 0.01 | 0.78 | 0.01 | 197.71 | 12.10 | 0.53 | 0.010 | 370.10 | 19.12 | 1.80 | 0.29 | 11.87 | 0.16 | 2.13 | 0.15 | 0.15 | 0.02 | 5.59 | 0.41 | 6.28 | 0.52 | 3.55 | 0.26 | 28.02 | 2.09 | 11.96 | 1.00 | 0.45 | 0.01 | 0.38 | 0.01 | 47.27 | 4.93 | 0.22 | 0.02 | 33 | 20 | 339 | 7 | 7.73 | 0.10 | 847 | 6 | 776 | 7 | 728 | 6 | 36 | 1 | 11.1 | 0.2 | 8 | 10.87 | 331.68 | 332.81 | 736.65 | 33.91 | 781.43 | 0.53 | 0.82 |
laboratory | Emiliania huxleyi (phytoplankton) | 115104 | marinespecies.org | 2 | Ocean carbonation | 1.58 | 0.06 | 0.14 | 0.01 | 0.12 | 0.01 | 0.83 | 0.02 | 308.87 | 15.57 | 0.70 | 0.030 | 439.98 | 30.39 | 5.58 | 0.27 | 14.53 | 0.70 | 2.25 | 0.07 | 0.38 | 0.03 | 6.47 | 0.48 | 9.24 | 0.11 | 5.19 | 0.18 | 33.76 | 1.27 | 14.31 | 0.87 | 0.72 | 0.04 | 0.65 | 0.04 | 65.76 | 2.58 | 0.40 | 0.01 | 33 | 20 | 880 | 49 | 8.08 | 0.02 | 5229 | 17 | 4702 | 33 | 4205 | 46 | 468 | 16 | 28.7 | 1.6 | 8 | 28.10 | 857.78 | 860.70 | 4264.97 | 439.55 | 4732.62 | 6.91 | 10.67 |
laboratory | Emiliania huxleyi (phytoplankton) | 115104 | marinespecies.org | 2 | Ocean acidification | 1.27 | 0.02 | 0.12 | 0.01 | 0.08 | 0.01 | 0.69 | 0.01 | 241.18 | 7.21 | 0.60 | 0.031 | 402.49 | 23.21 | 2.65 | 0.18 | 11.44 | 0.46 | 1.81 | 0.05 | 0.23 | 0.02 | 6.34 | 0.26 | 5.86 | 0.42 | 4.61 | 0.17 | 27.13 | 1.54 | 16.14 | 0.81 | 0.78 | 0.04 | 0.58 | 0.03 | 51.92 | 3.59 | 0.42 | 0.02 | 33 | 20 | 901 | 14 | 7.72 | 0.01 | 2162 | 17 | 2050 | 17 | 1925 | 15 | 96 | 2 | 29.4 | 0.4 | 8 | 29.36 | 896.30 | 899.35 | 1945.32 | 87.52 | 2062.19 | 1.38 | 2.12 |