Citation:

PANGAEA.943274

Name: Seawater carbonate chemistry and growth rates, carbon utilization and the internal pH regulation at the site of calcification in Emiliania huxleyi, Calcidiscus leptoporus and Pleurochrysis
Published: 2021
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Acid-base regulation; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcidiscus leptoporus; Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Other studied parameter or process; Pelagos; Phytoplankton; Pleurochrysis carterae; Primary production/Photosynthesis; Single species

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Abstract:

Ocean acidification (OA) appears to have diverse impacts on calcareous coccolithophores, but the cellular processes underlying these responses are not well understood. Here we use stable boron and carbon isotopes, B/Ca ratios, as well as inorganic and organic carbon production rates to investigate the carbon utilization and the internal pH regulation at the site of calcification in Emiliania huxleyi, Pleurochrysis carterae and Calcidiscus leptoporus cultured over a wide pCO2 range (180 to 1000 μatm). Despite large variability, the geochemistry data indicate species-specific modes of pH control and differences in the utilization of inorganic carbon. Boron isotope data suggest that all three species generally upregulate the pH of the calcification fluid (pHCF) compared to surrounding seawater, which coincides with relatively constant growth rates and cellular ratios of inorganic to organic carbon. Furthermore, species exhibit different strategies in regulating their pHCF, i.e., two species maintain homeostasis (pHCF = ∼ 8.7), while one species shows a constant offset to the surrounding seawater (ΔpH = ∼0.6 units) over the entire tested pCO2 range. In addition to these different strategies, carbon isotope data suggests that high plasticity in the utilization of dissolved inorganic carbon might be an explanation for species-specific differences in coccolithophore responses to OA.

Keyword(s):

Acid-base regulation; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcidiscus leptoporus; Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Other studied parameter or process; Pelagos; Phytoplankton; Pleurochrysis carterae; Primary production/Photosynthesis; Single species

Supplement to:

Liu, Yiwei; Rokitta, Sebastian D; Rost, Björn; Eagle, Robert A (2021): Constraints on coccolithophores under ocean acidification obtained from boron and carbon geochemical approaches. Geochimica et Cosmochimica Acta, 315, 317-332, https://doi.org/10.1016/j.gca.2021.09.025

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-04-13.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Liu, Yiwei		study
2	Species	Species		Liu, Yiwei
3	Registration number of species	Reg spec no		Liu, Yiwei		WoRMS Aphia ID
4	Uniform resource locator/link to reference	URL ref		Liu, Yiwei
5	Identification	ID		Liu, Yiwei
6	pH	pH		Liu, Yiwei		NBS scale
7	pH, standard deviation	pH std dev	±	Liu, Yiwei		NBS scale
8	Growth rate	µ	1/day	Liu, Yiwei		Specific
9	Growth rate, standard deviation	µ std dev	±	Liu, Yiwei		Specific
10	Carbon, organic, particulate, per cell	POC/cell	pg/#	Liu, Yiwei
11	Carbon, organic, particulate, standard deviation	POC std dev	±	Liu, Yiwei
12	Carbon, inorganic, particulate, per cell	PIC/cell	pg/#	Liu, Yiwei
13	Particulate inorganic carbon per cell, standard deviation	PIC/cell std dev	±	Liu, Yiwei
14	Carbon, organic, particulate, production per cell	POC prod/cell	pg/#/day	Liu, Yiwei
15	Carbon, inorganic, particulate, production per cell	PIC prod/cell	pg/#/day	Liu, Yiwei
16	Particulate inorganic carbon/particulate organic carbon ratio	PIC/POC		Liu, Yiwei
17	Particulate inorganic carbon/particulate organic carbon ratio, standard deviation	PIC/POC ratio std dev	±	Liu, Yiwei
18	δ13C, particulate organic carbon	δ13C POC	‰ PDB	Liu, Yiwei
19	δ13C, particulate organic carbon, standard deviation	δ13C POC std dev	±	Liu, Yiwei
20	δ13C, particulate inorganic carbon	δ13C PIC	‰	Liu, Yiwei
21	δ13C, particulate inorganic carbon, standard deviation	δ13C PIC std dev	±	Liu, Yiwei
22	δ13C, dissolved inorganic carbon	δ13C DIC	‰ PDB	Liu, Yiwei		t0
23	δ13C, dissolved inorganic carbon	δ13C DIC	‰ PDB	Liu, Yiwei		tfin
24	δ13C, dissolved inorganic carbon	δ13C DIC	‰ PDB	Liu, Yiwei		average
25	δ13C, dissolved inorganic carbon, standard deviation	δ13C DIC std dev	±	Liu, Yiwei
26	Difference δ13C, particulate organic carbon and dissolved inorganic carbon	δ13C POC - δ13C DIC	‰	Liu, Yiwei
27	Difference δ13C, particulate organic carbon and dissolved inorganic carbon, standard deviation	δ13C POC - δ13C DIC std dev	±	Liu, Yiwei		2 SD
28	Difference δ13C, particulate inorganic carbon and dissolved inorganic carbon	δ13C PIC - δ13C DIC	‰	Liu, Yiwei
29	Difference δ13C, particulate inorganic carbon and dissolved inorganic carbon, standard deviation	δ13C PIC - δ13C DIC std dev	±	Liu, Yiwei		2 SD
30	δ11B	δ11B	‰ SRM	Liu, Yiwei
31	δ11B, standard deviation	δ11B std dev	±	Liu, Yiwei		2 SD
32	Calcifying fluid, pH	CF pH		Liu, Yiwei
33	Calcifying fluid, pH, standard deviation	CF pH std dev	±	Liu, Yiwei		2 SD
34	pH, difference	Delta pH		Liu, Yiwei
35	pH, difference, standard deviation	Delta pH std dev	±	Liu, Yiwei		2 SD
36	Boron/Calcium ratio	B/Ca	µmol/mol	Liu, Yiwei
37	Boron/Calcium ratio, standard deviation	B/Ca std dev	±	Liu, Yiwei		2RSD
38	Magnesium/Calcium ratio	Mg/Ca	mmol/mol	Liu, Yiwei
39	Magnesium/Calcium ratio, standard deviation	Mg/Ca std dev	±	Liu, Yiwei		2RSD
40	Iron/Calcium ratio	Fe/Ca	µmol/mol	Liu, Yiwei
41	Iron/Calcium ratio, standard deviation	Fe/Ca std dev	±	Liu, Yiwei		2RSD
42	Salinity	Sal		Liu, Yiwei
43	pH	pH		Liu, Yiwei	Potentiometric	NBS scale
44	pH, standard deviation	pH std dev	±	Liu, Yiwei	Potentiometric	NBS scale
45	Temperature, water	Temp	°C	Liu, Yiwei
46	Temperature, water, standard deviation	Temp std dev	±	Liu, Yiwei
47	Carbon, inorganic, dissolved	DIC	µmol/kg	Liu, Yiwei	Calculated using CO2SYS
48	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Liu, Yiwei	Calculated using CO2SYS
49	Alkalinity, total	AT	µmol/kg	Liu, Yiwei	Potentiometric titration
50	Alkalinity, total, standard deviation	AT std dev	±	Liu, Yiwei	Potentiometric titration
51	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
53	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
54	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
55	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
56	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
57	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
58	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
59	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
60	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1945 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no (WoRMS Aphia ID)	4 URL ref	5 ID	6 pH (NBS scale)	7 pH std dev [±] (NBS scale)	8 µ [1/day] (Specific)	9 µ std dev [±] (Specific)	10 POC/cell [pg/#]	11 POC std dev [±]	12 PIC/cell [pg/#]	13 PIC/cell std dev [±]	14 POC prod/cell [pg/#/day]	15 PIC prod/cell [pg/#/day]	16 PIC/POC	17 PIC/POC ratio std dev [±]	18 δ13C POC [‰ PDB]	19 δ13C POC std dev [±]	20 δ13C PIC [‰]	21 δ13C PIC std dev [±]	22 δ13C DIC [‰ PDB] (t0)	23 δ13C DIC [‰ PDB] (tfin)	24 δ13C DIC [‰ PDB] (average)	25 δ13C DIC std dev [±]	26 δ13C POC - δ13C DIC [‰]	27 δ13C POC - δ13C DIC std dev [±] (2 SD)	28 δ13C PIC - δ13C DIC [‰]	29 δ13C PIC - δ13C DIC std dev [±] (2 SD)	30 δ11B [‰ SRM]	31 δ11B std dev [±] (2 SD)	32 CF pH	33 CF pH std dev [±] (2 SD)	34 Delta pH	35 Delta pH std dev [±] (2 SD)	36 B/Ca [µmol/mol]	37 B/Ca std dev [±] (2RSD)	38 Mg/Ca [mmol/mol]	39 Mg/Ca std dev [±] (2RSD)	40 Fe/Ca [µmol/mol]	41 Fe/Ca std dev [±] (2RSD)	42 Sal	43 pH (NBS scale, Potentiometric)	44 pH std dev [±] (NBS scale, Potentiometric)	45 Temp [°C]	46 Temp std dev [±]	47 DIC [µmol/kg] (Calculated using CO2SYS)	48 DIC std dev [±] (Calculated using CO2SYS)	49 AT [µmol/kg] (Potentiometric titration)	50 AT std dev [±] (Potentiometric titration)	51 CSC flag (Calculated using seacarb afte...)	52 pH (total scale, Calculated using...)	53 CO2 [µmol/kg] (Calculated using seacarb afte...)	54 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	55 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	56 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	57 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	58 AT [µmol/kg] (Calculated using seacarb afte...)	59 Omega Arg (Calculated using seacarb afte...)	60 Omega Cal (Calculated using seacarb afte...)
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	EH180A	8.321	0.007	0.77	0.07	14.79	0.20	11.73	0.43	11.40	9.04	0.79	0.03	-32.23	0.04	-13.280	0.00	-14.80	-14.89	-14.84	0.06	-17.39	0.15	1.56	0.13	22.56	1.66	8.48	0.14	0.16	0.14	19.00	2.28	0.14	0.02	157.49	11.02	34.10	8.29	0.01	17.17	0.84	1955.86	38.47	2294.09	20.47	27	8.17	9.77	276.75	277.73	1741.28	204.85	2248.80	3.17	4.92
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	EH180B	8.351	0.021	0.76	0.08	16.31	0.08	12.46	0.28	12.41	9.48	0.76	0.02	-38.81	0.13	-19.890	0.00	-21.81	-21.12	-21.47	0.49	-17.34	1.01	1.58	0.98							20.34	2.44	0.43	0.06	101.03	7.07	34.10	8.32	0.01	17.23	0.91	1912.70	83.08	2267.47	49.63	27	8.20	8.85	251.27	252.16	1690.60	213.25	2220.30	3.30	5.12
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	EH180C	8.342	0.012	0.78	0.01	18.52	0.40	14.57	0.42	14.49	11.40	0.79	0.03	-38.13	0.01	-19.620	0.00	-21.11	-20.74	-20.93	0.26	-17.20	0.52	1.31	0.52	25.85	1.90	8.69	0.11	0.35	0.12	13.40	1.61	0.29	0.04	91.34	6.39	34.10	8.31	0.01	17.23	0.77	1951.47	66.79	2296.16	18.20	27	8.19	9.26	262.74	263.67	1728.94	213.30	2257.00	3.30	5.12
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	EH380A	8.111	0.003	0.85	0.04	21.13	0.63	16.22	0.95	17.99	13.80	0.77	0.05	-28.18	0.02	-7.180	0.01	-9.08	-8.53	-8.81	0.39	-19.37	0.77	1.62	0.77	28.74	1.66	8.88	0.11	0.77	0.11							34.10	8.08	0.00	17.10	0.44	2050.12	42.88	2275.83	40.48	27	7.96	17.20	486.27	487.99	1895.22	137.68	2236.60	2.13	3.31
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	EH380B	8.094	0.007	0.94	0.07	16.16	0.18	10.93	0.23	15.12	10.22	0.68	0.02	-28.88	0.21	-6.650	0.01	-8.32	-8.29	-8.31	0.02	-20.57	0.41	1.66	0.03	23.49	1.66	8.54	0.13	0.45	0.13							34.10	8.06	0.01	17.62	0.75	2052.31	36.73	2281.05	34.76	27	7.94	18.05	518.10	519.92	1901.16	133.09	2231.10	2.06	3.20
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	EH380C	8.101	0.017	0.89	0.02	18.03	0.28	11.23	0.75	15.99	9.95	0.62	0.04	-32.22	0.02	-12.250	0.01	-14.20	-13.71	-13.96	0.34	-18.26	0.69	1.71	0.69							20.19	2.42	0.32	0.04	106.02	7.42	34.10	8.07	0.01	17.37	0.84	2052.07	41.27	2274.45	41.99	27	7.95	17.72	504.97	506.74	1899.57	134.81	2233.80	2.09	3.24
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	EH750A	7.887	0.024	0.90	0.08	13.19	0.04	9.59	0.11	11.81	8.59	0.73	0.01	-40.31	0.11	-22.460	0.00	-24.05	-23.67	-23.86	0.27	-16.45	0.58	1.40	0.54							19.11	2.29	0.28	0.04	398.24	27.88	34.10	7.86	0.02	17.22	0.57	2169.86	20.70	2283.78	31.77	27	7.74	31.11	882.75	885.87	2049.61	89.18	2270.00	1.38	2.14
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	EH750B	7.882	0.026	0.83	0.03	18.57	0.07	11.67	0.53	15.40	9.68	0.63	0.03	-40.28	0.42	-23.240	0.00	-25.24	-24.69	-24.96	0.39	-15.32	1.14	1.72	0.78	22.43	1.66	8.47	0.14	0.59	0.15	19.81	2.38	0.22	0.03	105.30	7.37	34.10	7.86	0.02	17.03	0.59	2162.18	24.58	2280.40	35.46	27	7.74	31.33	884.03	887.15	2043.13	87.74	2260.20	1.36	2.11
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	EH750C	7.878	0.028	0.82	0.07	15.71	0.74	11.80	0.76	12.81	9.62	0.75	0.06	-42.70	0.04	-23.430	0.01	-25.61	-24.95	-25.28	0.47	-17.42	0.94	1.85	0.93	22.27	1.90	8.46	0.12	0.58	0.13	22.53	2.70	0.28	0.04	111.42	7.80	34.10	7.85	0.02	17.40	0.77	2158.89	28.45	2276.46	39.79	27	7.73	31.65	902.83	906.01	2040.15	87.09	2255.60	1.35	2.09
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	EH1000A	7.764	0.021	0.73	0.08	13.16	0.18	10.59	2.08	9.62	7.75	0.81	0.16	-39.61	0.56	-20.490	0.00	-22.41	-22.26	-22.34	0.10	-17.27	1.13	1.84	0.21	29.72	1.66	8.94	0.11	1.18	0.12	22.45	2.69	0.31	0.04	186.53	13.06	34.10	7.74	0.02	17.20	0.44	2192.17	25.38	2301.69	12.14	27	7.62	41.86	1186.93	1191.12	2081.97	68.38	2251.20	1.06	1.64
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	EH1000B	7.800	0.065	0.92	0.17	11.31	0.34	7.81	0.36	10.41	7.18	0.69	0.04	-43.40	0.10	-23.530	0.01	-24.82	-24.84	-24.83	0.01	-18.57	0.21	1.29	0.03													34.10	7.77	0.06	17.22	0.64	2160.96	72.40	2259.63	58.22	27	7.65	37.96	1077.11	1080.91	2049.93	73.11	2231.20	1.13	1.76
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	EH1000C	7.771	0.025	0.84	0.03	17.74	0.50	9.46	1.00	14.86	7.92	0.53	0.06	-40.33	0.13	-23.670	0.01	-25.70	-25.32	-25.51	0.27	-14.82	0.60	1.84	0.53	20.80	1.90	8.36	0.13	0.59	0.14							34.10	7.74	0.02	17.53	0.77	2189.74	45.68	2280.66	35.18	27	7.62	41.17	1178.83	1182.98	2078.87	69.66	2251.20	1.08	1.67
laboratory	Calcidiscus leptoporus (phytoplankton)	235923	marinespecies.org	CL180A	8.361	0.028	0.39		63.91	0.23	31.20	5.83	24.78	12.10	0.49	0.09	-36.17	0.15	-21.247	0.01	-19.20	-18.81	-19.00	0.28	-17.16	0.64	-2.24	0.56	35.13	1.90	9.40	0.21	1.04	0.21	119.95	4.80	11.59	1.53	539.63	37.77	33.90	8.33	0.02	17.40	0.77	1986.59	7.48	2321.10	18.81	27	8.21	9.02	256.92	257.83	1752.64	224.94	2306.60	3.49	5.41
laboratory	Calcidiscus leptoporus (phytoplankton)	235923	marinespecies.org	CL180B	8.355	0.015	0.37		62.68	1.22	34.55	4.64	23.12	12.75	0.55	0.07	-34.86	0.37	-18.991	0.02	-16.79	-16.40	-16.60	0.28	-18.26	0.93	-2.39	0.55	19.45	1.90	8.26	0.15	-0.10	0.15							33.90	8.33	0.01	17.02	0.46	1981.47	7.75	2316.70	13.99	27	8.21	9.09	256.25	257.15	1751.07	221.34	2296.80	3.43	5.32
laboratory	Calcidiscus leptoporus (phytoplankton)	235923	marinespecies.org	CL180C	8.350	0.011	0.39		57.34	1.39	31.20	7.43	22.64	12.32	0.54	0.13	-34.69	0.02	-19.757	0.01	-17.80	-17.76	-17.78	0.03	-16.92	0.07	-1.98	0.06							75.83	3.03	7.43	0.98	966.03	67.62	33.90	8.32	0.01	16.97	0.51	1982.20	5.34	2316.34	13.60	27	8.20	9.20	258.76	259.68	1753.66	219.35	2294.60	3.40	5.27
laboratory	Calcidiscus leptoporus (phytoplankton)	235923	marinespecies.org	CL380A	8.128	0.018	0.38	0.05	65.64	2.72	30.54	3.55	24.87	11.57	0.47	0.06	-35.38	0.07	-17.950	0.01	-16.61	-16.60	-16.61	0.01	-18.78	0.14	-1.34	0.03	27.14	1.90	8.78	0.11	0.65	0.12							33.90	8.10	0.01	17.17	0.70	2081.94	12.56	2311.88	8.71	27	7.98	16.82	476.13	477.81	1920.78	144.30	2277.00	2.24	3.47
laboratory	Calcidiscus leptoporus (phytoplankton)	235923	marinespecies.org	CL380B	8.122	0.021	0.38	0.06	73.06	9.63	34.10	13.44	27.79	12.97	0.47	0.19	-35.09	1.01			-19.03	-19.04	-19.03	0.01	-16.06	2.02															33.90	8.09	0.01	17.25	0.71	2085.36	5.61	2313.85	10.87	27	7.97	17.14	486.10	487.81	1925.69	142.57	2277.60	2.21	3.43
laboratory	Calcidiscus leptoporus (phytoplankton)	235923	marinespecies.org	CL380C	8.120	0.021	0.34	0.07	65.25	2.42	34.63	7.66	21.93	11.64	0.53	0.12	-35.14	0.19			-15.86	-15.60	-15.73	0.18	-19.42	0.52															33.90	8.09	0.02	17.10	0.44	2086.82	6.68	2308.02	4.49	27	7.97	17.19	485.61	487.33	1927.57	142.04	2278.20	2.20	3.42
laboratory	Calcidiscus leptoporus (phytoplankton)	235923	marinespecies.org	CL750A	7.870	0.023	0.43	0.17	54.38	0.88	24.40	4.00	23.64	10.61	0.45	0.07	-43.03	0.08	-25.518	0.01	-25.21	-25.01	-25.11	0.14	-17.92	0.33	-0.41	0.28	34.95	1.90	9.38	0.20	1.51	0.20							33.90	7.84	0.01	17.35	0.71	2215.34	13.89	2319.95	17.56	27	7.72	33.16	943.40	946.72	2094.82	87.32	2309.70	1.35	2.10
laboratory	Calcidiscus leptoporus (phytoplankton)	235923	marinespecies.org	CL750B	7.864	0.028	0.45	0.10	54.89	1.62	35.87	2.65	24.60	16.08	0.65	0.05	-39.70	0.51			-25.40	-24.73	-25.06	0.48	-14.64	1.39															33.90	7.84	0.02	16.93	0.40	2214.61	6.14	2311.95	8.79	27	7.72	33.51	941.81	945.15	2095.21	85.87	2306.70	1.33	2.07
laboratory	Calcidiscus leptoporus (phytoplankton)	235923	marinespecies.org	CL750C	7.839	0.024	0.42	0.12	56.37	2.00	37.46	6.50	23.70	15.75	0.66	0.12	-40.68	0.42	-25.053	0.01	-25.22	-24.81	-25.01	0.29	-15.67	1.02	-0.04	0.57	28.35	1.90	8.86	0.12	1.02	0.13							33.90	7.81	0.02	17.15	0.60	2213.15	4.01	2309.15	5.72	27	7.69	35.55	1005.46	1009.01	2096.24	81.31	2296.60	1.26	1.96
laboratory	Calcidiscus leptoporus (phytoplankton)	235923	marinespecies.org	CL1000A	7.757	0.010	0.21	0.14	155.56	3.33	41.86	3.35	33.25	8.95	0.27	0.02	-36.04	0.24			-25.93	-25.29	-25.61	0.45	-10.43	1.02									82.12	3.28	0.68	0.09	636.03	44.52	33.90	7.73	0.00	17.25	0.71	2205.10	27.78	2324.08	22.07	27	7.61	42.88	1216.28	1220.57	2094.80	67.43	2261.40	1.04	1.62
laboratory	Calcidiscus leptoporus (phytoplankton)	235923	marinespecies.org	CL1000B	7.762	0.028	0.25	0.07	71.91	1.75	53.68	6.52	18.12	13.52	0.75	0.09	-38.10	0.28			-26.10	-25.55	-25.82	0.39	-12.28	0.96															33.90	7.74	0.02	17.18	0.57	2203.39	14.69	2324.85	22.92	27	7.62	42.38	1199.73	1203.97	2092.99	68.03	2261.10	1.05	1.64
laboratory	Calcidiscus leptoporus (phytoplankton)	235923	marinespecies.org	CL1000C	7.762	0.028	0.34	0.02	61.58	0.17	31.29	4.17	21.04	10.70	0.51	0.07	-40.57	0.27			-25.31	-25.37	-25.34	0.04	-15.23	0.54															33.90	7.73	0.02	17.38	0.71	2212.90	5.34	2324.73	22.79	27	7.61	42.64	1214.17	1218.44	2101.86	68.40	2270.70	1.06	1.65
laboratory	Pleurochrysis carterae (phytoplankton)	235969	marinespecies.org	PC180D	8.399	0.043	0.77	0.07	128.96	7.44	23.19	9.19	99.14	17.83	0.18	0.07	-31.59	0.17	-9.330	0.01	-9.98	-9.00	-9.49	0.69	-22.10	1.43	0.16	1.38	28.35	1.90	8.88	0.12	0.48	0.14							30.80	8.37	0.04	16.90	0.48	1971.71	33.93	2352.18	1.13	27	8.25	8.49	234.35	235.18	1740.05	223.16	2283.90	3.50	5.48
laboratory	Pleurochrysis carterae (phytoplankton)	235969	marinespecies.org	PC180E	8.381	0.035	0.88	0.54	176.20	2.27	26.65	9.60	155.41	23.51	0.15	0.05	-30.63	0.04	-7.490	0.01									35.49	1.90	9.46	0.22	1.08	0.23							30.80	8.36	0.03	16.72	0.53	1988.25	23.78	2356.81	7.76	27	8.23	8.94	245.41	246.28	1762.41	216.85	2290.70	3.40	5.32
laboratory	Pleurochrysis carterae (phytoplankton)	235969	marinespecies.org	PC180F	8.374	0.035	0.81	0.50	166.80	14.44	32.58	15.40	135.29	26.43	0.20	0.09	-31.41	0.82	-7.970	0.01	-8.51	-8.64	-8.58	0.09	-22.83	1.66	0.61	0.18	33.11	1.90	9.22	0.15	0.85	0.17							30.80	8.35	0.03	17.04	0.47	1990.20	25.11	2354.52	4.63	27	8.22	9.16	253.76	254.65	1767.15	213.90	2288.20	3.36	5.25
laboratory	Pleurochrysis carterae (phytoplankton)	235969	marinespecies.org	PC380D	8.173	0.073	0.85	0.65	125.98	2.51	28.80	11.00	107.60	24.60	0.23	0.09	-32.15	0.24	-6.510	0.00	-6.27	-6.22	-6.24	0.04	-25.91	0.48	-0.27	0.07	29.89	1.90	8.98	0.12	0.80	0.19	46.18	0.92	2.09	0.28	388.53	27.20	30.80	8.15	0.07	16.98	0.44	2076.04	24.31	2392.40	37.66	27	8.02	15.70	434.34	435.88	1914.08	146.22	2270.90	2.29	3.59
laboratory	Pleurochrysis carterae (phytoplankton)	235969	marinespecies.org	PC380E	8.146	0.079	0.86	0.58	141.61	4.20	16.98	9.44	121.21	14.53	0.12	0.07	-32.41	0.03	-6.520	0.01	-6.62	-5.83	-6.22	0.56	-26.18	1.12	-0.30	1.12	28.72	1.90	8.90	0.12	0.75	0.20	61.82	1.24	7.20	0.95	899.58	62.97	30.80	8.12	0.07	17.00	1.00	2084.92	37.67	2353.80	3.64	27	8.00	16.84	466.24	467.88	1929.51	138.54	2267.70	2.17	3.40
laboratory	Pleurochrysis carterae (phytoplankton)	235969	marinespecies.org	PC380F	8.169	0.068	0.88	0.42	127.06	0.39	13.66	16.21	111.98	12.03	0.11	0.13	-32.09	0.45	-6.210	0.01	-7.49	-6.48	-6.99	0.71	-25.11	1.69	0.77	1.42	26.19	1.66	8.74	0.12	0.57	0.18							30.80	8.14	0.05	17.13	1.06	2076.09	32.33	2357.49	6.95	27	8.02	15.87	441.14	442.70	1915.13	145.09	2269.20	2.28	3.56
laboratory	Pleurochrysis carterae (phytoplankton)	235969	marinespecies.org	PC750D	7.904	0.083	0.97	0.48	144.13	1.20	29.37	7.63	139.25	28.37	0.20	0.05	-46.74	0.15	-21.150	0.01	-21.52	-21.11	-21.31	0.29	-25.43	0.65	0.17	0.57	19.19	1.66	8.25	0.20	0.35	0.26							30.80	7.88	0.07	16.92	0.85	2201.20	20.04	2355.88	6.49	27	7.76	31.66	874.35	877.44	2083.69	85.85	2292.90	1.35	2.11
laboratory	Pleurochrysis carterae (phytoplankton)	235969	marinespecies.org	PC750E	7.923	0.084	0.84	0.37	137.64	0.00	24.34	6.06	115.12	20.36	0.18	0.04	-42.98	0.13	-17.440	0.01	-18.25	-17.80	-18.02	0.32	-24.95	0.70	0.59	0.64	26.79	1.66	8.78	0.11	0.85	0.20	14.03	0.28	0.88	0.12	225.33	15.77	30.80	7.90	0.07	17.15	0.82	2198.51	31.79	2363.43	13.45	27	7.77	30.30	842.43	845.40	2078.62	89.58	2296.80	1.41	2.20
laboratory	Pleurochrysis carterae (phytoplankton)	235969	marinespecies.org	PC750F	7.938	0.103	0.90	0.56	168.03	5.62	25.15	5.62	151.49	22.67	0.15	0.03	-34.69	0.08	-7.850	0.00	-7.30	-6.39	-6.84	0.65	-27.85	1.30	-1.00	1.29	18.89	1.66	8.23	0.16	0.29	0.26	75.80	1.52	2.41	0.32	560.25	39.22	30.80	7.91	0.09	17.37	1.17	2187.30	37.67	2354.21	3.36	27	7.78	29.18	816.69	819.56	2065.93	92.19	2290.50	1.45	2.26
laboratory	Pleurochrysis carterae (phytoplankton)	235969	marinespecies.org	PC1000D	7.821	0.070	0.74	0.14	159.57	4.58	32.36	8.75	117.85	23.90	0.20	0.06	-36.96	0.06	-8.760	0.01	-8.02	-7.46	-7.74	0.40	-29.21	0.80	-1.02	0.79	17.64	1.66	8.11	0.29	0.29	0.33							30.80	7.80	0.06	16.66	0.63	2183.54	28.85	2353.35	3.02	27	7.68	37.94	1039.88	1043.57	2074.82	70.74	2247.90	1.11	1.74
laboratory	Pleurochrysis carterae (phytoplankton)	235969	marinespecies.org	PC1000E	7.838	0.086	0.72	0.01	179.74	2.48	29.75	5.00	128.62	21.29	0.17	0.03	-41.89	0.36	-13.800	0.01	-13.61	-12.77	-13.19	0.59	-28.70	1.39	-0.61	1.19	20.10	1.90	8.33	0.14	0.49	0.22	79.66	1.59	0.67	0.09	311.11	21.78	30.80	7.82	0.08	16.83	0.62	2181.44	32.33	2351.54	0.44	27	7.69	36.51	1005.58	1009.14	2071.42	73.47	2251.10	1.15	1.80
laboratory	Pleurochrysis carterae (phytoplankton)	235969	marinespecies.org	PC1000F	7.827	0.079	0.77	0.36	140.03	0.00	33.16	7.41	107.13	25.37	0.24	0.05	-42.62	0.00	-15.280	0.01	-14.86	-14.39	-14.63	0.33	-27.99	0.67	-0.65	0.67	27.37	1.90	8.81	0.11	0.99	0.19	142.96	2.86	1.55	0.21	586.00	41.02	30.80	7.80	0.07	16.88	0.71	2186.32	16.30	2358.92	8.51	27	7.68	37.61	1037.36	1041.03	2076.94	71.76	2252.40	1.13	1.76