Citation:

PANGAEA.943227

Name: Seawater carbonate chemistry and biomarkers related to metabolic potential, antioxidant capacity, cellular damage and energetic fitness in two life stages (juvenile and adult)
Published: 2018
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Benthic animals; Benthos; Brackish waters; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea brasiliana; Crassostrea gigas; Laboratory experiment; Mollusca; Other metabolic rates; Other studied parameter or process; Single species; South Atlantic; Temperate

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook TwitterShow Map Google Earth

Abstract:

Environmental hypercapnia in shallow coastal marine ecosystems can be exacerbated by increasing levels of atmospheric CO2. In these ecosystems organisms are expected to become increasingly subjected to pCO2 levels several times higher than those inhabiting ocean waters (e.g.: 10,000 µatm), but still our current understanding on different species capacity to respond to such levels of hypercapnia is limited. Oysters are among the most important foundation species inhabiting these coastal ecosystems, although natural oyster banks are increasingly threatened worldwide. In the present study we studied the effects of hypercapnia on two important oyster species, the pacific oyster C. gigas and the mangrove oyster C. brasiliana, to bring new insights on different species response mechanisms towards three hypercapnic levels (ca. 1,000; 4,000; 10,000 µatm), by study of a set of biomarkers related to metabolic potential (electron transport system – ETS), antioxidant capacity (SOD, CAT, GSH), cellular damage (LPO) and energetic fitness (GLY), in two life stages (juvenile and adult) after 28 days of exposure.

Results showed marked differences between each species tolerance capacity to hypercapnia, with contrasting metabolic readjustment strategies (ETS), different antioxidant response capacities (SOD, CAT, GSH), which generally allowed to prevent increased cellular damage (LPO) and energetic impairment (GLY) in both species. Juveniles were more responsive to hypercapnia stress in both congeners, and are likely to be most sensitive to extreme hypercapnia in the environment. Juvenile C. gigas presented more pronounced biochemical alterations at intermediate hypercapnia (4,000 µatm) than C. brasiliana. Adult C. gigas showed biochemical alterations mostly in response to high hypercapnia (10,000 µatm), while adult C. brasiliana were less responsive to this environmental stressor, despite presenting decreased metabolic potential.

Our data bring new insights on the biochemical performance of two important oyster species, and suggest that the duration of extreme hypercapnia events in the ecosystem may pose increased challenges for these organisms as their tolerance capacity may be time limited.

Keyword(s):

Animalia; Benthic animals; Benthos; Brackish waters; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea brasiliana; Crassostrea gigas; Laboratory experiment; Mollusca; Other metabolic rates; Other studied parameter or process; Single species; South Atlantic; Temperate

Related to:

Moreira, Anthony; Figueira, Etelvina; Pecora, Iracy L; Soares, Amadeu M V M; Freitas, Rosa (2018): Native and exotic oysters in Brazil: Comparative tolerance to hypercapnia. Environmental Research, 161, 202-211, https://doi.org/10.1016/j.envres.2017.10.035

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: -25.008200 * Longitude: -48.024800

Event(s):

Cananeia_estuary * Latitude: -25.008200 * Longitude: -48.024800 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-04-11.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Moreira, Anthony		study
2	Species	Species		Moreira, Anthony
3	Registration number of species	Reg spec no		Moreira, Anthony		WoRMS Aphia ID
4	Uniform resource locator/link to reference	URL ref		Moreira, Anthony
5	Experiment duration	Exp duration	days	Moreira, Anthony
6	Treatment	Treat		Moreira, Anthony
7	Stage	Stage		Moreira, Anthony
8	Electron transport system activity of oyxgen	ETS	µmol/g/min	Moreira, Anthony
9	Electron transport system activity of oyxgen, standard deviation	ETS std dev	±	Moreira, Anthony
10	Superoxide dismutase activity, unit per protein mass	SOD/prot	U/mg	Moreira, Anthony
11	Superoxide dismutase activity, standard deviation	SOD std dev	±	Moreira, Anthony
12	Catalase activity, unit per protein mass	CAT/prot	U/mg	Moreira, Anthony
13	Catalase activity, standard deviation	CAT std dev	±	Moreira, Anthony
14	Glutathione, total, reduced, per protein mass	TGSH/prot	nmol/mg	Moreira, Anthony
15	Glutathione, total, reduced, per protein mass, standard deviation	TGSH/prot std dev	±	Moreira, Anthony
16	Glutathione reduced/Glutathione oxidized, ratio	GSH/GSSG		Moreira, Anthony
17	Glutathione reduced/Glutathione oxidized, ratio, standard deviation	GSH/GSSG std dev	±	Moreira, Anthony
18	Lipid peroxidation, per wet mass	LPO	nmol/mg	Moreira, Anthony
19	Lipid peroxidation, standard deviation	LPO std dev	±	Moreira, Anthony
20	Glycogen	GLY	µg/mg	Moreira, Anthony
21	Glycogen, standard deviation	GLY std dev	±	Moreira, Anthony
22	Temperature, water	Temp	°C	Moreira, Anthony
23	Salinity	Sal		Moreira, Anthony
24	pH	pH		Moreira, Anthony	Potentiometric	NBS scale
25	pH, standard deviation	pH std dev	±	Moreira, Anthony	Potentiometric	NBS scale
26	Alkalinity, total	AT	µmol/kg	Moreira, Anthony	Potentiometric titration
27	Alkalinity, total, standard deviation	AT std dev	±	Moreira, Anthony	Potentiometric titration
28	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Moreira, Anthony	Calculated using CO2SYS
29	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Moreira, Anthony	Calculated using CO2SYS
30	Bicarbonate ion	[HCO3]-	µmol/kg	Moreira, Anthony	Calculated using CO2SYS
31	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Moreira, Anthony	Calculated using CO2SYS
32	Carbonate ion	[CO3]2-	µmol/kg	Moreira, Anthony	Calculated using CO2SYS
33	Carbonate ion, standard deviation	[CO3]2- std dev	±	Moreira, Anthony	Calculated using CO2SYS
34	Calcite saturation state	Omega Cal		Moreira, Anthony	Calculated using CO2SYS
35	Calcite saturation state, standard deviation	Omega Cal std dev	±	Moreira, Anthony	Calculated using CO2SYS
36	Aragonite saturation state	Omega Arg		Moreira, Anthony	Calculated using CO2SYS
37	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Moreira, Anthony	Calculated using CO2SYS
38	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
40	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

564 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no (WoRMS Aphia ID)	4 URL ref	5 Exp duration [days]	6 Treat	7 Stage	8 ETS [µmol/g/min]	9 ETS std dev [±]	10 SOD/prot [U/mg]	11 SOD std dev [±]	12 CAT/prot [U/mg]	13 CAT std dev [±]	14 TGSH/prot [nmol/mg]	15 TGSH/prot std dev [±]	16 GSH/GSSG	17 GSH/GSSG std dev [±]	18 LPO [nmol/mg]	19 LPO std dev [±]	20 GLY [µg/mg]	21 GLY std dev [±]	22 Temp [°C]	23 Sal	24 pH (NBS scale, Potentiometric)	25 pH std dev [±] (NBS scale, Potentiometric)	26 AT [µmol/kg] (Potentiometric titration)	27 AT std dev [±] (Potentiometric titration)	28 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	29 pCO2 std dev [±] (Calculated using CO2SYS)	30 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	31 [HCO3]- std dev [±] (Calculated using CO2SYS)	32 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	33 [CO3]2- std dev [±] (Calculated using CO2SYS)	34 Omega Cal (Calculated using CO2SYS)	35 Omega Cal std dev [±] (Calculated using CO2SYS)	36 Omega Arg (Calculated using CO2SYS)	37 Omega Arg std dev [±] (Calculated using CO2SYS)	38 CSC flag (Calculated using seacarb afte...)	39 pH (total scale, Calculated using...)	40 CO2 [µmol/kg] (Calculated using seacarb afte...)	41 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	42 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	43 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	44 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	45 DIC [µmol/kg] (Calculated using seacarb afte...)	46 Omega Arg (Calculated using seacarb afte...)	47 Omega Cal (Calculated using seacarb afte...)
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	pH 7.8	Adult	2.16	0.39	0.29	0.07	0.50	0.06	0.0355	0.0083	1.48	0.47	0.0126	3.69	8.00	1.68	24	25	7.78	0.03	2087	88	1182	76	1937	99	69.0	6.3	1.8	0.20	1.1	0.10	26	7.64	35.54	1157.84	1161.59	1925.69	67.28	2028.5	1.11	1.74
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	pH 7.8	Juvenile	2.69	0.71	0.27	0.07	0.44	0.10	0.0446	0.0073	1.86	0.39	0.0154	4.19	6.19	2.33	24	25	7.78	0.03	2087	88	1182	76	1937	99	69.0	6.3	1.8	0.20	1.1	0.10	26	7.64	35.54	1157.84	1161.59	1925.69	67.28	2028.5	1.11	1.74
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	pH 7.4	Adult	2.64	0.87	0.20	0.03	0.42	0.08	0.0368	0.0092	1.74	0.40	0.0119	2.70	9.45	3.21	24	25	7.38	0.02	2679	115	3927	305	2591	113	37.5	2.1	1.0	0.10	0.6	0.04	26	7.24	120.38	3922.19	3934.95	2595.73	36.09	2752.2	0.60	0.94
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	pH 7.4	Juvenile	3.72	1.06	0.20	0.05	0.38	0.07	0.0486	0.0118	1.90	0.43	0.0198	5.03	7.43	3.11	24	25	7.38	0.02	2679	115	3927	305	2591	113	37.5	2.1	1.0	0.10	0.6	0.04	26	7.24	120.38	3922.19	3934.95	2595.73	36.09	2752.2	0.60	0.94
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	pH 7.0	Adult	3.06	1.14	0.26	0.07	0.51	0.09	0.0470	0.0053	1.78	0.30	0.0115	2.25	5.94	1.08	24	25	7.01	0.04	2881	107	10101	862	2840	105	17.7	1.9	0.5	0.05	0.3	0.03	26	6.87	309.20	10074.02	10107.03	2842.65	16.85	3168.7	0.28	0.44
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	pH 7.0	Juvenile	3.13	0.98	0.19	0.05	0.30	0.08	0.0338	0.0068	2.39	0.54	0.0155	3.90	7.50	1.82	24	25	7.01	0.04	2881	107	10101	862	2840	105	17.7	1.9	0.5	0.05	0.3	0.03	26	6.87	309.20	10074.02	10107.03	2842.65	16.85	3168.7	0.28	0.44
laboratory	Crassostrea brasiliana (mollusk)	506705	marinespecies.org	28	pH 7.8	Adult	5.93	1.08	0.09	0.03	0.38	0.04	0.0562	0.0053	1.60	0.20	0.0103	3.27	23.04	4.68	24	25	7.78	0.02	1919	109	1068	66	1764	101	63.6	5.2	1.7	0.14	1.1	0.09	26	7.64	32.64	1063.54	1066.99	1768.67	61.78	1863.1	1.02	1.60
laboratory	Crassostrea brasiliana (mollusk)	506705	marinespecies.org	28	pH 7.8	Juvenile	5.42	0.99	0.11	0.06	0.40	0.05	0.0539	0.0088	1.60	0.24	0.0171	3.62	20.25	7.08	24	25	7.78	0.02	1919	109	1068	66	1764	101	63.6	5.2	1.7	0.14	1.1	0.09	26	7.64	32.64	1063.54	1066.99	1768.67	61.78	1863.1	1.02	1.60
laboratory	Crassostrea brasiliana (mollusk)	506705	marinespecies.org	28	pH 7.4	Adult	3.92	1.13	0.16	0.06	0.39	0.07	0.0546	0.0098	1.78	0.30	0.0120	2.05	17.75	6.30	24	25	7.38	0.04	2508	175	3751	423	2428	170	34.4	4.1	0.9	0.10	0.6	0.07	26	7.24	112.67	3670.87	3682.81	2429.36	33.77	2575.8	0.56	0.88
laboratory	Crassostrea brasiliana (mollusk)	506705	marinespecies.org	28	pH 7.4	Juvenile	4.33	0.79	0.11	0.06	0.35	0.04	0.0514	0.0155	1.72	0.18	0.0175	4.61	14.55	4.15	24	25	7.38	0.04	2508	175	3751	423	2428	170	34.4	4.1	0.9	0.10	0.6	0.07	26	7.24	112.67	3670.87	3682.81	2429.36	33.77	2575.8	0.56	0.88
laboratory	Crassostrea brasiliana (mollusk)	506705	marinespecies.org	28	pH 7.0	Adult	4.65	0.75	0.11	0.05	0.34	0.06	0.0495	0.0058	1.52	0.13	0.0105	1.87	16.70	4.58	24	25	7.00	0.04	2789	105	9992	1010	2751	104	16.7	1.5	0.4	0.04	0.3	0.03	26	6.86	306.38	9982.16	10014.86	2752.57	15.94	3074.9	0.26	0.41
laboratory	Crassostrea brasiliana (mollusk)	506705	marinespecies.org	28	pH 7.0	Juvenile	4.15	1.37	0.12	0.04	0.34	0.07	0.0479	0.0074	1.68	0.48	0.0124	2.09	23.34	5.88	24	25	7.00	0.04	2789	105	9992	1010	2751	104	16.7	1.5	0.4	0.04	0.3	0.03	26	6.86	306.38	9982.16	10014.86	2752.57	15.94	3074.9	0.26	0.41