Citation:

PANGAEA.942812

Name: Seawater carbonate chemistry and biodiversity of coral reef cryptobiota
Published: 2021
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Benthos; Coast and continental shelf; Community composition and diversity; Containers and aquaria (20-1000 L or < 1 m**2); Entire community; Laboratory experiment; North Pacific; Rocky-shore community; Temperature; Tropical

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Abstract:

Ocean-warming and acidification are predicted to reduce coral reef biodiversity, but the combined effects of these stressors on overall biodiversity are largely unmeasured. Here, we examined the individual and combined effects of elevated temperature (+2 °C) and reduced pH (−0.2 units) on the biodiversity of coral reef communities that developed on standardized sampling units over a 2-y mesocosm experiment. Biodiversity and species composition were measured using amplicon sequencing libraries targeting the cytochrome oxidase I (COI) barcoding gene. Ocean-warming significantly increased species richness relative to present-day control conditions, whereas acidification significantly reduced richness. Contrary to expectations, species richness in the combined future ocean treatment with both warming and acidification was not significantly different from the present-day control treatment. Rather than the predicted collapse of biodiversity under the dual stressors, we find significant changes in the relative abundance but not in the occurrence of species, resulting in a shuffling of coral reef community structure among the highly species-rich cryptobenthic community. The ultimate outcome of altered community structure for coral reef ecosystems will depend on species-specific ecological functions and community interactions. Given that most species on coral reefs are members of the understudied cryptobenthos, holistic research on reef communities is needed to accurately predict diversity–function relationships and ecosystem responses to future climate conditions.

Keyword(s):

Benthos; Coast and continental shelf; Community composition and diversity; Containers and aquaria (20-1000 L or < 1 m**2); Entire community; Laboratory experiment; North Pacific; Rocky-shore community; Temperature; Tropical

Supplement to:

Timmers, Molly A; Jury, Christopher P; Vicente, Jan; Bahr, Keisha D; Webb, M; Toonen, Robert J (2021): Biodiversity of coral reef cryptobiota shuffles but does not decline under the combined stressors of ocean warming and acidification. Proceedings of the National Academy of Sciences, 118(39), https://doi.org/10.1073/pnas.2103275118

Source:

Timmers, Molly A (2021): Cryptobiota_Climate_Change. https://github.com/CuriousFauna/Cryptobiota_Climate_Change

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-03-30.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Timmers, Molly A		study
2	Treatment	Treat		Timmers, Molly A
3	Identification	ID		Timmers, Molly A		ARMS Unit
4	Identification	ID		Timmers, Molly A		Mesocosm Name
5	Number of sequences	Sequence no	#	Timmers, Molly A		Raw
6	Number of sequences	Sequence no	#	Timmers, Molly A		Post Filtration
7	Number of sequences	Sequence no	#	Timmers, Molly A		Metazoa & Macroalgae
8	Number of sequences	Sequence no	#	Timmers, Molly A		Classified to Phylum
9	Number of sequences	Sequence no	#	Timmers, Molly A		Post Rarefied
10	Operational taxonomic unit	OTU	#	Timmers, Molly A		Raw
11	Operational taxonomic unit	OTU	#	Timmers, Molly A		Post Filtration
12	Operational taxonomic unit	OTU	#	Timmers, Molly A		Metazoa & Macroalgae
13	Operational taxonomic unit	OTU	#	Timmers, Molly A		Classified to Phylum
14	Species richness	S		Timmers, Molly A		total
15	Phylum	Phylum		Timmers, Molly A
16	Reads	Reads	#	Timmers, Molly A		phlya
17	Abundance	Abund		Timmers, Molly A		Relative, phlya reads
18	Proportion	Prop		Timmers, Molly A		Phlya reads relative to Control
19	Operational taxonomic unit	OTU	#	Timmers, Molly A
20	Abundance	Abund		Timmers, Molly A		Relative, MOTUs
21	Proportion	Prop		Timmers, Molly A		MOTUs relative to Control
22	Salinity	Sal		Timmers, Molly A
23	Salinity, standard deviation	Sal std dev	±	Timmers, Molly A
24	Temperature, water	Temp	°C	Timmers, Molly A
25	Temperature, water, standard deviation	Temp std dev	±	Timmers, Molly A
26	pH	pH		Timmers, Molly A	Spectrophotometric	total scale
27	pH, standard deviation	pH std dev	±	Timmers, Molly A	Spectrophotometric	total scale
28	Alkalinity, total	AT	µmol/kg	Timmers, Molly A	Potentiometric titration
29	Alkalinity, total, standard deviation	AT std dev	±	Timmers, Molly A	Potentiometric titration
30	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Timmers, Molly A	Calculated using CO2SYS
31	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Timmers, Molly A	Calculated using CO2SYS
32	Aragonite saturation state	Omega Arg		Timmers, Molly A	Calculated using CO2SYS
33	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Timmers, Molly A	Calculated using CO2SYS
34	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
37	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
39	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
41	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
43	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
45	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
47	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
49	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2010 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Treat	3 ID (ARMS Unit)	4 ID (Mesocosm Name)	5 Sequence no [#] (Raw)	6 Sequence no [#] (Post Filtration)	7 Sequence no [#] (Metazoa & Macroalgae)	8 Sequence no [#] (Classified to Phylum)	9 Sequence no [#] (Post Rarefied)	10 OTU [#] (Raw)	11 OTU [#] (Post Filtration)	12 OTU [#] (Metazoa & Macroalgae)	13 OTU [#] (Classified to Phylum)	14 S (total)	15 Phylum	16 Reads [#] (phlya)	17 Abund (Relative, phlya reads)	18 Prop (Phlya reads relative to Control)	19 OTU [#]	20 Abund (Relative, MOTUs)	21 Prop (MOTUs relative to Control)	22 Sal	23 Sal std dev [±]	24 Temp [°C]	25 Temp std dev [±]	26 pH (total scale, Spectrophotometric)	27 pH std dev [±] (total scale, Spectrophotometric)	28 AT [µmol/kg] (Potentiometric titration)	29 AT std dev [±] (Potentiometric titration)	30 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	31 pCO2 std dev [±] (Calculated using CO2SYS)	32 Omega Arg (Calculated using CO2SYS)	33 Omega Arg std dev [±] (Calculated using CO2SYS)	34 CSC flag (Calculated using seacarb afte...)	35 CO2 [µmol/kg] (Calculated using seacarb afte...)	36 CO2 std dev [±] (Calculated using seacarb afte...)	37 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	38 fCO2 std dev [±] (Calculated using seacarb afte...)	39 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	40 pCO2 std dev [±] (Calculated using seacarb afte...)	41 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	42 [HCO3]- std dev [±] (Calculated using seacarb afte...)	43 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	44 [CO3]2- std dev [±] (Calculated using seacarb afte...)	45 DIC [µmol/kg] (Calculated using seacarb afte...)	46 DIC std dev [±] (Calculated using seacarb afte...)	47 Omega Arg (Calculated using seacarb afte...)	48 Omega Arg std dev [±] (Calculated using seacarb afte...)	49 Omega Cal (Calculated using seacarb afte...)	50 Omega Cal std dev [±] (Calculated using seacarb afte...)
laboratory	Control	1	Control10	99151	98102	70588	64252	16135	532	161	86	53	53								34.24	0.35	25.10	1.24	7.99	0.06	2176	54	447	61	2.88	0.34	8	12.50	2.15	439.69	74.14	441.10	74.38	1729.55	68.36	180.28	21.34	1922.33	59.64	2.88	0.35	4.37	0.52
laboratory	Control	2	Control17	79252	78290	54390	43857	16137	613	208	119	81	81								34.24	0.35	25.10	1.24	7.99	0.06	2176	54	447	61	2.88	0.34	8	12.50	2.15	439.69	74.14	441.10	74.38	1729.55	68.36	180.28	21.34	1922.33	59.64	2.88	0.35	4.37	0.52
laboratory	Control	3	Control23	41939	41552	34504	20539	16133	417	154	103	65	65								34.24	0.35	25.10	1.24	7.99	0.06	2176	54	447	61	2.88	0.34	8	12.50	2.15	439.69	74.14	441.10	74.38	1729.55	68.36	180.28	21.34	1922.33	59.64	2.88	0.35	4.37	0.52
laboratory	Control	4	Control26	133359	131426	87586	69356	16133	800	217	120	82	82								34.24	0.35	25.10	1.24	7.99	0.06	2176	54	447	61	2.88	0.34	8	12.50	2.15	439.69	74.14	441.10	74.38	1729.55	68.36	180.28	21.34	1922.33	59.64	2.88	0.35	4.37	0.52
laboratory	Control	5	Control9	188537	186078	121869	84384	16131	790	183	106	63	63								34.24	0.35	25.10	1.24	7.99	0.06	2176	54	447	61	2.88	0.34	8	12.50	2.15	439.69	74.14	441.10	74.38	1729.55	68.36	180.28	21.34	1922.33	59.64	2.88	0.35	4.37	0.52
laboratory	Acidified	6	Acid1	147690	146290	102861	90211	16133	590	138	74	47	47								34.24	0.35	25.12	1.23	7.78	0.07	2184	51	798	128	1.93	0.30	8	22.08	4.14	777.11	143.69	779.59	144.15	1884.57	62.58	121.21	17.74	2027.87	57.07	1.93	0.29	2.94	0.43
laboratory	Acidified	7	Acid15	58971	58450	47430	38223	16133	421	130	72	49	49								34.24	0.35	25.12	1.23	7.78	0.07	2184	51	798	128	1.93	0.30	8	22.08	4.14	777.11	143.69	779.59	144.15	1884.57	62.58	121.21	17.74	2027.87	57.07	1.93	0.29	2.94	0.43
laboratory	Acidified	8	Acid25	23080	22810	16867	16132	16132	372	161	89	59	59								34.24	0.35	25.12	1.23	7.78	0.07	2184	51	798	128	1.93	0.30	8	22.08	4.14	777.11	143.69	779.59	144.15	1884.57	62.58	121.21	17.74	2027.87	57.07	1.93	0.29	2.94	0.43
laboratory	Acidified	9	Acid36	136750	135074	96455	61968	16131	629	144	85	56	56								34.24	0.35	25.12	1.23	7.78	0.07	2184	51	798	128	1.93	0.30	8	22.08	4.14	777.11	143.69	779.59	144.15	1884.57	62.58	121.21	17.74	2027.87	57.07	1.93	0.29	2.94	0.43
laboratory	Acidified	10	Acid39	87137	86311	74351	51037	16135	489	134	80	54	54								34.24	0.35	25.12	1.23	7.78	0.07	2184	51	798	128	1.93	0.30	8	22.08	4.14	777.11	143.69	779.59	144.15	1884.57	62.58	121.21	17.74	2027.87	57.07	1.93	0.29	2.94	0.43
laboratory	Acidified	11	Acid7	76079	75351	59197	54573	16132	453	129	67	43	43								34.24	0.35	25.12	1.23	7.78	0.07	2184	51	798	128	1.93	0.30	8	22.08	4.14	777.11	143.69	779.59	144.15	1884.57	62.58	121.21	17.74	2027.87	57.07	1.93	0.29	2.94	0.43
laboratory	Heated	12	Heat18	227257	222986	139375	114585	16131	1133	260	159	111	111								34.27	0.35	27.05	1.20	7.98	0.06	2188	51	455	62	3.06	0.35	8	12.27	2.12	453.06	76.79	454.48	77.03	1722.19	67.77	188.51	21.96	1922.97	57.74	3.04	0.36	4.58	0.54
laboratory	Heated	13	Heat22	108989	107753	94682	87232	16132	637	185	123	90	90								34.27	0.35	27.05	1.20	7.98	0.06	2188	51	455	62	3.06	0.35	8	12.27	2.12	453.06	76.79	454.48	77.03	1722.19	67.77	188.51	21.96	1922.97	57.74	3.04	0.36	4.58	0.54
laboratory	Heated	14	Heat3	329916	325855	237876	133258	16135	1084	226	157	97	97								34.27	0.35	27.05	1.20	7.98	0.06	2188	51	455	62	3.06	0.35	8	12.27	2.12	453.06	76.79	454.48	77.03	1722.19	67.77	188.51	21.96	1922.97	57.74	3.04	0.36	4.58	0.54
laboratory	Heated	15	Heat31	489606	483185	429862	348066	16132	1164	216	146	101	101								34.27	0.35	27.05	1.20	7.98	0.06	2188	51	455	62	3.06	0.35	8	12.27	2.12	453.06	76.79	454.48	77.03	1722.19	67.77	188.51	21.96	1922.97	57.74	3.04	0.36	4.58	0.54
laboratory	Heated	16	Heat8	217388	214452	197158	153143	16133	884	223	134	93	93								34.27	0.35	27.05	1.20	7.98	0.06	2188	51	455	62	3.06	0.35	8	12.27	2.12	453.06	76.79	454.48	77.03	1722.19	67.77	188.51	21.96	1922.97	57.74	3.04	0.36	4.58	0.54
laboratory	Acidified-Heated	17	AcidHeat16	86486	85588	39209	34850	16130	501	159	77	50	50								34.27	0.35	27.11	1.50	7.77	0.07	2197	50	819	129	2.06	0.31	8	21.74	4.11	803.75	149.31	806.26	149.78	1883.19	63.56	127.38	18.81	2032.30	56.91	2.05	0.31	3.10	0.46
laboratory	Acidified-Heated	18	AcidHeat20	69353	68626	48010	43121	16137	493	199	109	78	78								34.27	0.35	27.11	1.50	7.77	0.07	2197	50	819	129	2.06	0.31	8	21.74	4.11	803.75	149.31	806.26	149.78	1883.19	63.56	127.38	18.81	2032.30	56.91	2.05	0.31	3.10	0.46
laboratory	Acidified-Heated	19	AcidHeat21	35957	35738	30917	26358	16127	307	167	109	81	81								34.27	0.35	27.11	1.50	7.77	0.07	2197	50	819	129	2.06	0.31	8	21.74	4.11	803.75	149.31	806.26	149.78	1883.19	63.56	127.38	18.81	2032.30	56.91	2.05	0.31	3.10	0.46
laboratory	Acidified-Heated	20	AcidHeat24	107760	106702	78706	58257	16132	541	179	115	80	80								34.27	0.35	27.11	1.50	7.77	0.07	2197	50	819	129	2.06	0.31	8	21.74	4.11	803.75	149.31	806.26	149.78	1883.19	63.56	127.38	18.81	2032.30	56.91	2.05	0.31	3.10	0.46
laboratory	Acidified-Heated	21	AcidHeat33	71096	70374	66717	35147	16135	480	143	99	63	63								34.27	0.35	27.11	1.50	7.77	0.07	2197	50	819	129	2.06	0.31	8	21.74	4.11	803.75	149.31	806.26	149.78	1883.19	63.56	127.38	18.81	2032.30	56.91	2.05	0.31	3.10	0.46
laboratory	Acidified-Heated	22	AcidHeat4	176210	174243	140163	128079	16132	701	162	94	57	57								34.27	0.35	27.11	1.50	7.77	0.07	2197	50	819	129	2.06	0.31	8	21.74	4.11	803.75	149.31	806.26	149.78	1883.19	63.56	127.38	18.81	2032.30	56.91	2.05	0.31	3.10	0.46
laboratory	Control													Annelida	26166	28.67	1.00	47	64.38	1.00	34.24	0.35	25.10	1.24	7.99	0.06	2176	54	447	61	2.88	0.34	8	12.50	2.15	439.69	74.14	441.10	74.38	1729.55	68.36	180.28	21.34	1922.33	59.64	2.88	0.35	4.37	0.52
laboratory	Acidified													Annelida	26758	29.32	1.02	28	38.36	0.60	34.24	0.35	25.12	1.23	7.78	0.07	2184	51	798	128	1.93	0.30	8	22.08	4.14	777.11	143.69	779.59	144.15	1884.57	62.58	121.21	17.74	2027.87	57.07	1.93	0.29	2.94	0.43
laboratory	Heated													Annelida	18412	20.18	0.70	56	76.71	1.19	34.27	0.35	27.05	1.20	7.98	0.06	2188	51	455	62	3.06	0.35	8	12.27	2.12	453.06	76.79	454.48	77.03	1722.19	67.77	188.51	21.96	1922.97	57.74	3.04	0.36	4.58	0.54
laboratory	Acidified-Heated													Annelida	19925	21.83	0.76	46	63.01	0.98	34.27	0.35	27.11	1.50	7.77	0.07	2197	50	819	129	2.06	0.31	8	21.74	4.11	803.75	149.31	806.26	149.78	1883.19	63.56	127.38	18.81	2032.30	56.91	2.05	0.31	3.10	0.46
laboratory	Control													Arthropoda	3549	11.31	1.00	33	50	1.00	34.24	0.35	25.10	1.24	7.99	0.06	2176	54	447	61	2.88	0.34	8	12.50	2.15	439.69	74.14	441.10	74.38	1729.55	68.36	180.28	21.34	1922.33	59.64	2.88	0.35	4.37	0.52
laboratory	Acidified													Arthropoda	11427	36.42	3.22	31	46.97	0.94	34.24	0.35	25.12	1.23	7.78	0.07	2184	51	798	128	1.93	0.30	8	22.08	4.14	777.11	143.69	779.59	144.15	1884.57	62.58	121.21	17.74	2027.87	57.07	1.93	0.29	2.94	0.43
laboratory	Heated													Arthropoda	6738	21.48	1.90	41	62.12	1.24	34.27	0.35	27.05	1.20	7.98	0.06	2188	51	455	62	3.06	0.35	8	12.27	2.12	453.06	76.79	454.48	77.03	1722.19	67.77	188.51	21.96	1922.97	57.74	3.04	0.36	4.58	0.54
laboratory	Acidified-Heated													Arthropoda	9658	30.79	2.72	38	57.58	1.15	34.27	0.35	27.11	1.50	7.77	0.07	2197	50	819	129	2.06	0.31	8	21.74	4.11	803.75	149.31	806.26	149.78	1883.19	63.56	127.38	18.81	2032.30	56.91	2.05	0.31	3.10	0.46
laboratory	Control													Cnidaria	4011	28.85	1.00	10	52.63	1.00	34.24	0.35	25.10	1.24	7.99	0.06	2176	54	447	61	2.88	0.34	8	12.50	2.15	439.69	74.14	441.10	74.38	1729.55	68.36	180.28	21.34	1922.33	59.64	2.88	0.35	4.37	0.52
laboratory	Acidified													Cnidaria	3391	24.39	0.85	6	31.58	0.60	34.24	0.35	25.12	1.23	7.78	0.07	2184	51	798	128	1.93	0.30	8	22.08	4.14	777.11	143.69	779.59	144.15	1884.57	62.58	121.21	17.74	2027.87	57.07	1.93	0.29	2.94	0.43
laboratory	Heated													Cnidaria	3597	25.87	0.90	12	63.16	1.20	34.27	0.35	27.05	1.20	7.98	0.06	2188	51	455	62	3.06	0.35	8	12.27	2.12	453.06	76.79	454.48	77.03	1722.19	67.77	188.51	21.96	1922.97	57.74	3.04	0.36	4.58	0.54
laboratory	Acidified-Heated													Cnidaria	2903	20.88	0.72	8	42.11	0.80	34.27	0.35	27.11	1.50	7.77	0.07	2197	50	819	129	2.06	0.31	8	21.74	4.11	803.75	149.31	806.26	149.78	1883.19	63.56	127.38	18.81	2032.30	56.91	2.05	0.31	3.10	0.46
laboratory	Control													Echinodermata	9822	18.04	1.00	10	90.91	1.00	34.24	0.35	25.10	1.24	7.99	0.06	2176	54	447	61	2.88	0.34	8	12.50	2.15	439.69	74.14	441.10	74.38	1729.55	68.36	180.28	21.34	1922.33	59.64	2.88	0.35	4.37	0.52
laboratory	Acidified													Echinodermata	20736	38.1	2.11	11	100	1.10	34.24	0.35	25.12	1.23	7.78	0.07	2184	51	798	128	1.93	0.30	8	22.08	4.14	777.11	143.69	779.59	144.15	1884.57	62.58	121.21	17.74	2027.87	57.07	1.93	0.29	2.94	0.43
laboratory	Heated													Echinodermata	5894	10.83	0.60	11	100	1.10	34.27	0.35	27.05	1.20	7.98	0.06	2188	51	455	62	3.06	0.35	8	12.27	2.12	453.06	76.79	454.48	77.03	1722.19	67.77	188.51	21.96	1922.97	57.74	3.04	0.36	4.58	0.54
laboratory	Acidified-Heated													Echinodermata	17979	33.03	1.83	10	90.91	1.00	34.27	0.35	27.11	1.50	7.77	0.07	2197	50	819	129	2.06	0.31	8	21.74	4.11	803.75	149.31	806.26	149.78	1883.19	63.56	127.38	18.81	2032.30	56.91	2.05	0.31	3.10	0.46
laboratory	Control													Mollusca	13120	22.86	1.00	15	62.5	1.00	34.24	0.35	25.10	1.24	7.99	0.06	2176	54	447	61	2.88	0.34	8	12.50	2.15	439.69	74.14	441.10	74.38	1729.55	68.36	180.28	21.34	1922.33	59.64	2.88	0.35	4.37	0.52
laboratory	Acidified													Mollusca	5526	9.63	0.42	13	54.17	0.87	34.24	0.35	25.12	1.23	7.78	0.07	2184	51	798	128	1.93	0.30	8	22.08	4.14	777.11	143.69	779.59	144.15	1884.57	62.58	121.21	17.74	2027.87	57.07	1.93	0.29	2.94	0.43
laboratory	Heated													Mollusca	25929	45.17	1.98	22	91.67	1.47	34.27	0.35	27.05	1.20	7.98	0.06	2188	51	455	62	3.06	0.35	8	12.27	2.12	453.06	76.79	454.48	77.03	1722.19	67.77	188.51	21.96	1922.97	57.74	3.04	0.36	4.58	0.54
laboratory	Acidified-Heated													Mollusca	12824	22.34	0.98	13	54.17	0.87	34.27	0.35	27.11	1.50	7.77	0.07	2197	50	819	129	2.06	0.31	8	21.74	4.11	803.75	149.31	806.26	149.78	1883.19	63.56	127.38	18.81	2032.30	56.91	2.05	0.31	3.10	0.46
laboratory	Control													Porifera	19886	34.26	1.00	18	43.9	1.00	34.24	0.35	25.10	1.24	7.99	0.06	2176	54	447	61	2.88	0.34	8	12.50	2.15	439.69	74.14	441.10	74.38	1729.55	68.36	180.28	21.34	1922.33	59.64	2.88	0.35	4.37	0.52
laboratory	Acidified													Porifera	12582	21.67	0.63	20	48.78	1.11	34.24	0.35	25.12	1.23	7.78	0.07	2184	51	798	128	1.93	0.30	8	22.08	4.14	777.11	143.69	779.59	144.15	1884.57	62.58	121.21	17.74	2027.87	57.07	1.93	0.29	2.94	0.43
laboratory	Heated													Porifera	16508	28.44	0.83	29	70.73	1.61	34.27	0.35	27.05	1.20	7.98	0.06	2188	51	455	62	3.06	0.35	8	12.27	2.12	453.06	76.79	454.48	77.03	1722.19	67.77	188.51	21.96	1922.97	57.74	3.04	0.36	4.58	0.54
laboratory	Acidified-Heated													Porifera	9075	15.63	0.46	19	46.34	1.06	34.27	0.35	27.11	1.50	7.77	0.07	2197	50	819	129	2.06	0.31	8	21.74	4.11	803.75	149.31	806.26	149.78	1883.19	63.56	127.38	18.81	2032.30	56.91	2.05	0.31	3.10	0.46
laboratory	Control													Rhodophyta	3825	24.98	1.00	17	53.13	1.00	34.24	0.35	25.10	1.24	7.99	0.06	2176	54	447	61	2.88	0.34	8	12.50	2.15	439.69	74.14	441.10	74.38	1729.55	68.36	180.28	21.34	1922.33	59.64	2.88	0.35	4.37	0.52
laboratory	Acidified													Rhodophyta	155	1.01	0.04	5	15.63	0.29	34.24	0.35	25.12	1.23	7.78	0.07	2184	51	798	128	1.93	0.30	8	22.08	4.14	777.11	143.69	779.59	144.15	1884.57	62.58	121.21	17.74	2027.87	57.07	1.93	0.29	2.94	0.43
laboratory	Heated													Rhodophyta	3542	23.13	0.93	25	78.13	1.47	34.27	0.35	27.05	1.20	7.98	0.06	2188	51	455	62	3.06	0.35	8	12.27	2.12	453.06	76.79	454.48	77.03	1722.19	67.77	188.51	21.96	1922.97	57.74	3.04	0.36	4.58	0.54
laboratory	Acidified-Heated													Rhodophyta	7792	50.88	2.04	19	59.38	1.12	34.27	0.35	27.11	1.50	7.77	0.07	2197	50	819	129	2.06	0.31	8	21.74	4.11	803.75	149.31	806.26	149.78	1883.19	63.56	127.38	18.81	2032.30	56.91	2.05	0.31	3.10	0.46