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Posadas, Niño; Baquiran, Jake Ivan P; Nada, Michael Angelou L; Kelly, Michelle; Conaco, Cecilia (2021): Seawater carbonate chemistry and bacterial diversity and immune repertoire of the demosponge, Neopetrosia compacta, and the calcareous sponge, Leucetta chagosensis [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.942405

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Abstract:
The sponge-associated microbial community contributes to the overall health and adaptive capacity of the sponge holobiont. This community is regulated by the environment and the immune system of the host. However, little is known about the effect of environmental stress on the regulation of host immune functions and how this may, in turn, affect sponge–microbe interactions. In this study, we compared the bacterial diversity and immune repertoire of the demosponge, Neopetrosia compacta, and the calcareous sponge, Leucetta chagosensis, under varying levels of acidification and warming stress based on climate scenarios predicted for 2100. Neopetrosia compacta harbors a diverse microbial community and possesses a rich repertoire of scavenger receptors while L. chagosensis has a less diverse microbiome and an expanded range of pattern recognition receptors and immune response-related genes. Upon exposure to RCP 8.5 conditions, the microbiome composition and host transcriptome of N. compacta remained stable, which correlated with high survival (75%). In contrast, tissue necrosis and low survival (25%) of L. chagosensis was accompanied by microbial community shifts and downregulation of host immune-related pathways. Meta-analysis of microbiome diversity and immunological repertoire across poriferan classes further highlights the importance of host–microbe interactions in predicting the fate of sponges under future ocean conditions.
Keyword(s):
Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Community composition and diversity; Entire community; Gene expression (incl. proteomics); Immunology/Self-protection; Laboratory experiment; Leucetta chagosensis; Mortality/Survival; Neopetrosia compacta; North Pacific; Porifera; Single species; Temperature; Tropical
Supplement to:
Posadas, Niño; Baquiran, Jake Ivan P; Nada, Michael Angelou L; Kelly, Michelle; Conaco, Cecilia (2022): Microbiome diversity and host immune functions influence survivorship of sponge holobionts under future ocean conditions. The ISME Journal, 16(1), 58-67, https://doi.org/10.1038/s41396-021-01050-5
Original version:
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html
Coverage:
Latitude: 16.296000 * Longitude: 120.014000
Date/Time Start: 2018-09-01T00:00:00 * Date/Time End: 2018-09-30T00:00:00
Event(s):
Bolinao_Anda_Reef_Complex * Latitude: 16.296000 * Longitude: 120.014000 * Date/Time Start: 2018-09-01T00:00:00 * Date/Time End: 2018-09-30T00:00:00 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-3-14.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeConaco, Ceciliastudy
2SpeciesSpeciesConaco, Cecilia
3Registration number of speciesReg spec noConaco, CeciliaWoRMS Aphia ID
4Uniform resource locator/link to referenceURL refConaco, Cecilia
5FigureFigConaco, Cecilia
6TypeTypeConaco, Cecilia
7ReplicateReplConaco, Cecilia
8TreatmentTreatConaco, Cecilia
9Time in daysTimedaysConaco, Cecilia
10StatusStatusConaco, Cecilia0 alive, 1 dead
11OrderOrderConaco, Cecilia
12AbundanceAbund%Conaco, CeciliaRelative
13Species richnessSConaco, Cecilia
14Shannon Diversity IndexH'Conaco, Cecilia
15Diversity indexDiv indexConaco, CeciliaFisher
16Simpson index of diversitySimpson IConaco, Cecilia
17Inverse Simpson index of diversityInv Simpson IConaco, Cecilia
18IdentificationIDConaco, CeciliaASV
19FamilyFamilyConaco, Cecilia
20Fold changeFold changeConaco, Cecilialog
21NameNameConaco, CeciliaPathway
22AbundanceAbund%Conaco, CeciliaRelative
23GroupGroupConaco, CeciliaPattern
24IdentificationIDConaco, CeciliaGO
25GroupGroupConaco, CeciliaTerm
26Fold changeFold changeConaco, Cecilia
27Gene nameGeneConaco, Cecilia
28Numbern#Conaco, CeciliaOrthologs
29Gene expressionGene expressionConaco, CeciliaRelative
30Gene expressionGene expressionConaco, CeciliaDifferentially
31SalinitySalConaco, Cecilia
32Alkalinity, totalATµmol/kgConaco, Cecilia
33Carbon, inorganic, dissolvedDICµmol/kgConaco, Cecilia
34pH, total scalepHTConaco, Ceciliatotal scale
35Temperature, waterTemp°CConaco, CeciliaSample
36Carbon, inorganic, totalTICµmol/kgConaco, Cecilia
37Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmConaco, CeciliaCalculated using CO2SYS
38Carbon dioxideCO2µmol/kgConaco, CeciliaCalculated using CO2SYS
39Bicarbonate ion[HCO3]-µmol/kgConaco, CeciliaCalculated using CO2SYS
40Carbonate ion[CO3]2-µmol/kgConaco, CeciliaCalculated using CO2SYS
41Calcite saturation stateOmega CalConaco, CeciliaCalculated using CO2SYS
42Aragonite saturation stateOmega ArgConaco, CeciliaCalculated using CO2SYS
43Revelle factorRConaco, CeciliaCalculated using CO2SYS
44Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
45Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
46Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
47Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
48Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
49Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
50Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
51Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
52Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
38509 data points

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