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Beaufort, Luc; Probert, Ian; Suchéras-Marx, Baptiste (2022): Coccolith production through coccolithophore growth experiment [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.942383

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Published: 2022-03-15 • DOI registered: 2022-05-03

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Abstract:
Experiment measuring mass of a single coccolithophore cell every minute for near 9h. The coccolithophore is a Gephyrocapsa huxleyi culture strain RCC1216 from the Roscoff Culture Collection. A multi-well plate containing a low-density culture of calcifying cells grown in K/2 culture medium (Keller et al., 1987) was placed on the stage of an inverted microscope Leica DMi8 with a Smarteck GC3851MP camera, installed in a climate-controlled room (20°C) at the Biological Station of Roscoff and illuminated with large-spectrum white LED lights with an intensity of ca. 100 µmol.m-2.s-1. The coccolithophore produced 9 coccoliths during the experiment. The mass of individual coccospheres (in pg CaCO3) was estimated from their birefringence quantified from the images (Beaufort et al., 2014). Each produced coccolith was heavier than the previous one. The cell was most probably in G1 interphase and thus growing during the experiment.
Keyword(s):
coccolith; coccolithophore; culture experiment; life cycle
Related to:
Suchéras-Marx, Baptiste; Viseur, Sophie; Walker, Charlotte E; Beaufort, Luc; Probert, Ian; Bolton, Clara T (2022): Coccolith size rules - What controls the size of coccoliths during coccolithogenesis? Marine Micropaleontology, 170, 102080, https://doi.org/10.1016/j.marmicro.2021.102080
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 Quality flag, timeQF timeSuchéras-Marx, Baptistesee Beaufort et al. (2014)Time (decimal hour)
2 MassMasspgSuchéras-Marx, Baptistesee Beaufort et al. (2014)
3 Sample commentSample commentSuchéras-Marx, Baptistesee Beaufort et al. (2014)Coccolith construction number
4 Sample commentSample commentSuchéras-Marx, Baptistesee Beaufort et al. (2014)Coccolith number expulsion
Change history:
2022-04-04T05:27:29 – The abstract has been updated at the author request.
License:
Creative Commons Attribution-ShareAlike 4.0 International (CC-BY-SA-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1612 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
QF time
(Time (decimal hour))		2 
Mass [pg]		3 
Sample comment
(Coccolith construction number)		4 
Sample comment
(Coccolith number expulsion)
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