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Howald, Sarah; Moyano, Marta; Crespel, Amélie; Kuchenmüller, Luis L; Cominassi, Louise; Claireaux, Guy; Peck, Myron A; Mark, Felix Christopher (2022): Experimental conditions for respiration and growth studies of F0 and F1 larval and juvenile European seabass Dicentrarchus labrax [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.941766, In: Howald, S et al. (2022): Respiration and growth rates of F0 and F1 larval and juvenile European seabass Dicentrarchus labrax in response to ocean acidification and warming [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.941767

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Abstract:
Water parameters in the 2 years before spawning of F0 (08.02.2016-06.03.2018) and during larval and juvenile phase of F1: Larval period until 17.05.2018 (48 dph, 900 dd) and 01.06.2018 (63 dph, ~900 dd) for warm and cold life condition respectively, for the juveniles until 28.09.2018 (180 dph, ~4000 dd) and 12.02.2019 (319 dph, ~5100 dd) for warm and cold conditioned fish respectively. Means ± s.e. over all replicate tanks per condition. Temperature (Temp.), pH (free scale), salinity, oxygen and total alkalinity (TA) were measured weekly in F1 and monthly in F0; sea water (SW) measurements were conducted in 2017 and 2018. Water parameters during larval and early juvenile phase of F0: Larval period until (45 dph, 900 dd, 06.12.2013), early juveniles until 1.5 years. Means ± s.e.m. over all measurements per condition (triplicate tanks in larvae, single tanks in juveniles). Temperature (Temp.) and pH (NBS scale) were measured daily. pH (total scale), salinity, phosphate, silicate and total alkalinity (TA) were measured once at the beginning and once at the end of the larval phase and 9 times during juvenile phase; PCO2 was calculated with CO2sys; A–Ambient PCO2, D1000 –ambient + 1000 µatm CO2, L – Larvae, J – Juveniles.
Keyword(s):
juvenile growth; Laboratory experiment; larval growth; metabolic rates; Ocean acidification; ocean warming; teleost
Supplement to:
Howald, Sarah; Moyano, Marta; Crespel, Amélie; Cominassi, Louise; Claireaux, Guy; Peck, Myron A; Mark, Felix Christopher (2021): Transgenerational effects decrease larval resilience to ocean acidification & warming but juvenile European sea bass could benefit from higher temperatures in the NE Atlantic. bioRxiv, 2021.11.15.468704, https://doi.org/10.1101/2021.11.15.468704
Howald, Sarah; Moyano, Marta; Crespel, Amélie; Kuchenmüller, Luis L; Cominassi, Louise; Claireaux, Guy; Peck, Myron A; Mark, Felix Christopher (2022): Effects of ocean acidification over successive generations decrease resilience of larval European sea bass to ocean acidification and warming but juveniles could benefit from higher temperatures in the NE Atlantic. Journal of Experimental Biology, 225(9), https://doi.org/10.1242/jeb.243802
Coverage:
Date/Time Start: 2013-12-06T00:00:00 * Date/Time End: 2019-02-12T00:00:00
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
Type of studyStudy typeHowald, Sarah
DATE/TIMEDate/TimeHowald, SarahGeocode – start
DATE/TIMEDate/TimeHowald, SarahGeocode – end
TreatmentTreatHowald, Sarah
GenerationGeneration#Howald, Sarah
Life stageLife stageHowald, Sarah
pHpHHowald, SarahpH meter (WTW 3110) with electrode (WTW Sentix 41)NBS scale
pH, standard errorpH std e±Howald, SarahCalculated
pHpHHowald, SarahMeasured spectrophotometrically (Dickson et al., 2007) with purified m-cresol purpletotal scale
10 pH, standard errorpH std e±Howald, SarahCalculated
11 pHpHHowald, SarahpH meter (WTW 3110) with electrode (WTW Sentix 41)free scale
12 pH, standard errorpH std e±Howald, SarahCalculated
13 Temperature, waterTemp°CHowald, SarahMultiprobe, WTW 340i
14 Temperature, water, standard errorT std e±Howald, SarahCalculated
15 SalinitySalHowald, SarahSalinometer (WTW LF325, Xylem Analytics Germany, Weilheim, Germany)psu
16 Salinity, standard errorSal std e±Howald, SarahCalculated
17 Oxygen, dissolvedDO%Howald, SarahWTW Oxi 340i probe
18 Oxygen, dissolved, standard errorDO std e±Howald, SarahCalculated
19 Alkalinity, totalATµmol/lHowald, SarahCalculated by CO2sys_xls_program (Lewis and Wallace, 2006)
20 Alkalinity, total, standard errorAT std e±Howald, SarahCalculated
21 Carbon dioxide (water) partial pressurepCO2(aq)µatmHowald, SarahCalculated by CO2sys_xls_program (Lewis and Wallace, 2006)
22 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Howald, SarahCalculated
23 Phosphate[PO4]3-µmol/lHowald, SarahSEAL AA3 segmented flow autoanalyzer
24 Phosphate, standard errorPO4 std e±Howald, SarahCalculated
25 SilicateSi(OH)4µmol/lHowald, SarahSEAL AA3 segmented flow autoanalyzer
26 Silicate, standard errorSi(OH)4 std e±Howald, SarahCalculated
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
238 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Study type

Date/Time
(start)

Date/Time
(end)

Treat

Generation [#]

Life stage

pH
(NBS scale, pH meter (WTW 3110...)

pH std e [±]
(Calculated)

pH
(total scale, Measured spectro...)
10 
pH std e [±]
(Calculated)
11 
pH
(free scale, pH meter (WTW 311...)
12 
pH std e [±]
(Calculated)
13 
Temp [°C]
(Multiprobe, WTW 340i)
14 
T std e [±]
(Calculated)
15 
Sal
(psu, Salinometer (WTW LF325, ...)
16 
Sal std e [±]
(Calculated)
17 
DO [%]
(WTW Oxi 340i probe)
18 
DO std e [±]
(Calculated)
19 
AT [µmol/l]
(Calculated by CO2sys_xls_prog...)
20 
AT std e [±]
(Calculated)
21 
pCO2(aq) [µatm]
(Calculated by CO2sys_xls_prog...)
22 
pCO2water_SST_wet std e [±]
(Calculated)
23 
[PO4]3- [µmol/l]
(SEAL AA3 segmented flow autoa...)
24 
PO4 std e [±]
(Calculated)
25 
Si(OH)4 [µmol/l]
(SEAL AA3 segmented flow autoa...)
26 
Si(OH)4 std e [±]
(Calculated)
laboratory2013-12-06L A0Larva7.960.017.890.0119.20.333.80.222943589100.570.018.940.06
laboratory2013-12-06L Delta10000Larva7.590.007.540.0319.20.333.80.2230691521970.570.018.940.06
laboratoryJ A0Juvenile8.050.017.940.0315.30.134.30.2229410516310.710.088.350.26
laboratoryJ Delta10000Juvenile7.610.017.530.0215.30.134.30.22280161489420.710.088.350.26
laboratory2016-02-082018-03-06F0 A0Spawners7.950.0214.10.633.60.392.41.724064967040
laboratory2016-02-082018-03-06F0 Delta10000Spawners7.590.0214.10.633.60.392.41.9241146161674
laboratory2018-06-01F1 L C A1Larva8.060.0115.30.131.80.194.31.023602350419
laboratory2018-06-01F1 L C Delta10001Larva7.530.0115.50.131.80.194.30.8233022187274
laboratory2018-05-17F1 L W A1Larva7.960.0120.20.231.70.084.93.423113265622
laboratory2018-05-17F1 L W Delta10001Larva7.610.0120.20.231.80.088.11.7232132162459
laboratory2019-02-12F1 J C A1Juvenile7.940.0116.10.233.00.192.40.523761569619
laboratory2019-02-12F1 J C Delta10001Juvenile7.600.0116.30.233.00.194.30.5238014160332
laboratory2018-09-28F1 J W Delta10001Juvenile7.570.0222.70.233.00.286.31.3232316186683
ambientSW8.070.0115.00.534.60.3101.00.824412360937