Citation:

PANGAEA.941567

Name: Seawater carbonate chemistry and RNA:DNA ratios, SOD activity, catalase activity, and immunoblots of HSP 70 in Mytilus edulis
Creator: Michael P Lesser
Published: 2016
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Mollusca; Mytilus edulis; North Pacific; Other studied parameter or process; Single species; Temperate; Temperature

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Abstract:

The interactive effects of multiple stressors associated with global climate change will expose marine organisms to physiological challenges potentially exceeding their current abilities to acclimatize. As a result the ecological effects of the rapidly increasing magnitude, and variability, of environmental change combined with the unknown physiological capacity to acclimatize (= phenotypic plasticity) or genetically adapt, remains uncertain for many marine organisms. In the Gulf of Maine (GOM), the rocky intertidal harbors the blue mussel, Mytilus edulis, an important ecosystem engineer in these communities. Using mussel collections in the Southwest GOM from different tidal heights and a common garden experiment, mussels show significant, phenotypically plastic, effects of tidal height in multiple parameters related to metabolic capacity and stress tolerance. When these mussels are then experimentally exposed to the independent and interactive effects of thermal stress and ocean acidification, several biomarkers of stress (e.g., oxidative stress, HSP70, protein synthesis) are elevated in treatments with higher temperatures, but when combined with lower pH consistent with future predictions these markers show evidence of metabolic depression. In marine ecosystems, exposure to ocean acidification has been hypothesized as a factor that would narrow the thermal window of physiological tolerance for many invertebrates such as marine mussels. The data presented here provide evidence supporting that hypothesis in blue mussels from the GOM, an ecosystem facing one of the greatest rates of change in the marine environment.

Keyword(s):

Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Mollusca; Mytilus edulis; North Pacific; Other studied parameter or process; Single species; Temperate; Temperature

Supplement to:

Lesser, Michael P (2016): Climate change stressors cause metabolic depression in the blue mussel, Mytilus edulis, from the Gulf of Maine. Limnology and Oceanography, 61(5), 1705-1717, https://doi.org/10.1002/lno.10326

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 42.966600 * Longitude: -69.400000

Event(s):

Appledore_Island * Latitude: 42.966600 * Longitude: -69.400000 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-3-1.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Lesser, Michael P		study
2	Species	Species		Lesser, Michael P
3	Registration number of species	Reg spec no		Lesser, Michael P		WoRMS Aphia ID
4	Uniform resource locator/link to reference	URL ref		Lesser, Michael P
5	Experiment duration	Exp duration	days	Lesser, Michael P
6	Treatment	Treat		Lesser, Michael P
7	RNA/DNA ratio	RNA/DNA		Lesser, Michael P
8	RNA/DNA ratio, standard error	RNA/DNA std e	±	Lesser, Michael P
9	Citrate synthase activity, unit per protein mass	CS/prot	U/mg	Lesser, Michael P
10	Citrate synthase activity, standard error	CS act std e	±	Lesser, Michael P
11	Pyruvate kinase activity, per protein	PK act	U/g	Lesser, Michael P
12	Pyruvate kinase activity, per protein, standard error	PK act std e	±	Lesser, Michael P
13	Glycogen	GLY	µg/mg	Lesser, Michael P
14	Glycogen, standard error	GLY std e	±	Lesser, Michael P
15	δ13C	δ13C	‰ PDB	Lesser, Michael P
16	δ13C, standard error	δ13C std e	±	Lesser, Michael P
17	δ15N	δ15N	‰ air	Lesser, Michael P
18	δ15N, standard error	δ15N std e	±	Lesser, Michael P
19	Percentage	Perc	%	Lesser, Michael P		phytoplankton in diet
20	Percentage, standard error	Perc std e	±	Lesser, Michael P		phytoplankton in diet
21	Superoxide dismutase activity, unit per protein mass	SOD/prot	U/mg	Lesser, Michael P
22	Superoxide dismutase activity, standard error	SOD std e	±	Lesser, Michael P
23	Catalase activity, unit per protein mass	CAT/prot	U/mg	Lesser, Michael P
24	Catalase activity, per protein mass, standard error	CAT/prot std e	±	Lesser, Michael P
25	Condition index	CI		Lesser, Michael P
26	Condition index, standard error	CI std e	±	Lesser, Michael P
27	Proteins	Protein		Lesser, Michael P		SOD
28	Proteins, standard error	Protein std e	±	Lesser, Michael P		SOD
29	Proteins	Protein		Lesser, Michael P		HSP 70
30	Proteins, standard error	Protein std e	±	Lesser, Michael P		HSP 70
31	Proteins	Protein		Lesser, Michael P		p53
32	Proteins, standard error	Protein std e	±	Lesser, Michael P		p53
33	Temperature, water	Temp	°C	Lesser, Michael P
34	Temperature, water, standard deviation	Temp std dev	±	Lesser, Michael P
35	Salinity	Sal		Lesser, Michael P
36	Alkalinity, total	AT	µmol/kg	Lesser, Michael P
37	Alkalinity, total, standard deviation	AT std dev	±	Lesser, Michael P
38	Carbon, inorganic, dissolved	DIC	µmol/kg	Lesser, Michael P	Calculated using CO2calc
39	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Lesser, Michael P	Calculated using CO2calc
40	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Lesser, Michael P
41	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Lesser, Michael P
42	pH	pH		Lesser, Michael P	Calculated using CO2calc	total scale
43	Calcite saturation state	Omega Cal		Lesser, Michael P	Calculated using CO2calc
44	Aragonite saturation state	Omega Arg		Lesser, Michael P	Calculated using CO2calc
45	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
47	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

284 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no (WoRMS Aphia ID)	4 URL ref	5 Exp duration [days]	6 Treat	7 RNA/DNA	8 RNA/DNA std e [±]	9 CS/prot [U/mg]	10 CS act std e [±]	11 PK act [U/g]	12 PK act std e [±]	13 GLY [µg/mg]	14 GLY std e [±]	15 δ13C [‰ PDB]	16 δ13C std e [±]	17 δ15N [‰ air]	18 δ15N std e [±]	19 Perc [%] (phytoplankton in diet)	20 Perc std e [±] (phytoplankton in diet)	21 SOD/prot [U/mg]	22 SOD std e [±]	23 CAT/prot [U/mg]	24 CAT/prot std e [±]	25 CI	26 CI std e [±]	27 Protein (SOD)	28 Protein std e [±] (SOD)	29 Protein (HSP 70)	30 Protein std e [±] (HSP 70)	31 Protein (p53)	32 Protein std e [±] (p53)	33 Temp [°C]	34 Temp std dev [±]	35 Sal	36 AT [µmol/kg]	37 AT std dev [±]	38 DIC [µmol/kg] (Calculated using CO2calc)	39 DIC std dev [±] (Calculated using CO2calc)	40 pCO2water_SST_wet [µatm]	41 pCO2 std dev [±]	42 pH (total scale, Calculated using...)	43 Omega Cal (Calculated using CO2calc)	44 Omega Arg (Calculated using CO2calc)	45 CSC flag (Calculated using seacarb afte...)	46 pH (total scale, Calculated using...)	47 CO2 [µmol/kg] (Calculated using seacarb afte...)	48 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	49 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	50 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	51 DIC [µmol/kg] (Calculated using seacarb afte...)	52 Omega Arg (Calculated using seacarb afte...)	53 Omega Cal (Calculated using seacarb afte...)
laboratory	Mytilus edulis (mollusk)	140480	marinespecies.org	21	Low tidal height	3.96	0.17	27.20	1.86	11400	1290	317.0	13.72	-18.48	0.14	9.14	0.20	70.33	0.14	21.80	2.82	15.2	1.77	34.10	2.24	15.96	2.14	26.28	3.18	11.33	2.35
laboratory	Mytilus edulis (mollusk)	140480	marinespecies.org	21	Middle tidal height	3.55	0.06	14.20	1.39	19600	2890	283.8	4.66	-18.10	0.30	8.48	0.07	69.95	0.30	43.80	5.54	23.4	2.99	26.90	1.60	26.14	2.05	34.42	4.33	15.51	1.71
laboratory	Mytilus edulis (mollusk)	140480	marinespecies.org	21	High tidal height	1.76	0.11	5.60	1.44	27400	2420	141.2	9.11	-17.00	0.09	8.36	0.09	68.85	0.09	71.20	10.75	27.6	4.93	20.15	1.93	47.44	3.39	58.38	5.61	28.02	4.37
laboratory	Mytilus edulis (mollusk)	140480	marinespecies.org	21	Common garden	3.10	0.11	14.13	0.80	23330	1090	236.1	10.21	-18.22	0.12	8.85	0.10	70.07	0.12	42.47	2.89	23.6	1.29	31.33	1.30	26.60	1.35	28.42	2.64	15.48	0.82
laboratory	Mytilus edulis (mollusk)	140480	marinespecies.org	21	Ambient T and pH	4.00	0.10	22.70	1.30			311.6	12.00							32.50	2.60	20.7	1.20					28.40	2.60			15.2	1.6	30.8	2231	16	2031	19	379	15	8.067	3.619	2.304	24	8.07	14.39	377.63	1868.27	147.86	2030.52	2.31	3.62
laboratory	Mytilus edulis (mollusk)	140480	marinespecies.org	21	Ambient T and Low pH	3.20	0.10	17.60	1.10			141.7	11.60							31.70	2.20	23.7	1.00					33.40	2.80			15.2	1.6	30.8	2377	32	2223	26	561	29	7.943	3.023	1.924	24	7.94	21.31	558.98	2077.43	123.51	2222.25	1.93	3.03
laboratory	Mytilus edulis (mollusk)	140480	marinespecies.org	21	High T and Ambient pH	2.30	0.10	27.30	1.30			283.4	16.70							44.70	2.90	45.5	1.90					73.60	3.60			18.1	1.1	30.8	2198	23	1951	36	384	15	8.057	4.442	2.879	24	8.06	13.39	382.67	1813.78	156.68	1983.84	2.47	3.85
laboratory	Mytilus edulis (mollusk)	140480	marinespecies.org	21	High T and Low pH	1.50	0.10	11.90	0.50			169.9	16.60							64.00	4.10	31.5	1.60					43.90	2.30			18.1	1.1	30.8	2418	32	2207	12	561	29	7.954	4.034	2.614	24	7.95	19.56	559.05	2078.55	140.84	2238.94	2.22	3.46