Citation:

PANGAEA.941237

Name: Enzyme activity after drying and bedform migration in the Spree River in 2018/2019
Published: 2023-04-26
License: https://creativecommons.org/licenses/by/4.0/
Keywords: drought; microbial abundance; microbial community; ripples; sediment transport; streambed metabolism

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Abstract:

Sandy streambed sediments that had been exposed to either intense drying (90-day without rainfall) or bedform migration (periodically moving ripples) were placed in 250 µm mesh bags to the Spree River in Northeastern Germany on October 22, 2018, to initiate the recovery process from drying or migration stress. Subsamples from the recovering sediments were collected at eight dates within eight months and taken to the laboratory for further analysis. Potential extracellular enzyme activities (Beta-Glucosidase, Xylosidase, Chitinase, Phosphatase, Leucine-Aminopeptidase, Phenol-Oxidase, and Peroxidase) were assessed by measuring fluorescence and absorbance after extraction from sediments. The aim of the experiment was to observe the long-term recovery of microbial communities in lowland streams from drying and bedform migration and to identify if stress effects persisted as legacy after the stress period.

Keyword(s):

drought; microbial abundance; microbial community; ripples; sediment transport; streambed metabolism

Supplement to:

Oprei, Anna; Schreckinger, José; Kholiavko, Tatiana; Frossard, Aline; Mutz, Michael; Risse-Buhl, Ute (2023): Long-term functional recovery and associated microbial community structure after sediment drying and bedform migration. Frontiers in Ecology and Evolution, 11, 1112519, https://doi.org/10.3389/fevo.2023.1112519

Related to:

Mendoza-Lera, Clara; Frossard, Aline; Knie, Matthias; Federlein, Laura L; Gessner, Mark O; Mutz, Michael (2017): Importance of advective mass transfer and sediment surface area for streambed microbial communities. Freshwater Biology, 62(1), 133-145, https://doi.org/10.1111/fwb.12856

Perez-Mon, Carla; Frey, Beat; Frossard, Aline (2020): Functional and Structural Responses of Arctic and Alpine Soil Prokaryotic and Fungal Communities Under Freeze-Thaw Cycles of Different Frequencies. Frontiers in Microbiology, 11, https://doi.org/10.3389/fmicb.2020.00982

Winkler, J P; Cherry, R S; Schlesinger, W H (1996): The Q10 relationship of microbial respiration in a temperate forest soil. Soil Biology and Biochemistry, 28, 1067–1072

Project(s):

Overlooked bed shift? Modulation of the stream microbial food web and metabolism by patches of migrating sand ripples (EcoMigRip)

Funding:

Deutsche Forschungsgemeinschaft, Bonn (DFG), grant/award no. 400895888

Coverage:

Latitude: 51.833333 * Longitude: 14.333333

Date/Time Start: 2018-10-22T10:00:00 * Date/Time End: 2019-02-04T10:00:00

Minimum Elevation: 60.0 m * Maximum Elevation: 60.0 m

Event(s):

Spree_2018-2019 * Latitude: 51.833333 * Longitude: 14.333333 * Date/Time Start: 2018-10-22T10:00:00 * Date/Time End: 2019-06-03T10:00:00 * Elevation: 60.0 m * Location: Spree River near Döbbrick, Brandenburg, Germany * Method/Device: Experiment (EXP) * Comment: Exposition and collection site of sediment samples

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	DATE/TIME	Date/Time		Oprei, Anna		Geocode – Collection date of sediment samples
2	Time in days	Time	days	Oprei, Anna		Days after start of recovery
3	Treatment	Treat		Oprei, Anna
4	Beta-glucosidase activity	B-glu	µmol/h/ml	Schreckinger, José	MUB, Infinite 200 fluorometer (Tecan, Männedorf, Switzerland), 365/445 nm (excitation/emmission wavelength), standardized to 20°C, Q10 factor of 2 (Winkler et al. 1996)
5	Xylosidase activity	Xylo	µmol/h/ml	Schreckinger, José	MUB, Infinite 200 fluorometer (Tecan, Männedorf, Switzerland), 365/445 nm (excitation/emmission wavelength), standardized to 20°C, Q10 factor of 2 (Winkler et al. 1996)
6	Chitinase activity	Chit	µmol/h/ml	Schreckinger, José	MUB, Infinite 200 fluorometer (Tecan, Männedorf, Switzerland), 365/445 nm (excitation/emmission wavelength), standardized to 20°C, Q10 factor of 2 (Winkler et al. 1996)
7	Phosphatase activity	Phos	µmol/h/ml	Schreckinger, José	MUB, Infinite 200 fluorometer (Tecan, Männedorf, Switzerland), 365/445 nm (excitation/emmission wavelength), standardized to 20°C, Q10 factor of 2 (Winkler et al. 1996)
8	Leucine aminopeptidase activity	Leu aminopep	µmol/h/ml	Schreckinger, José	AMC, Infinite 200 fluorometer (Tecan, Männedorf, Switzerland), 365/450 nm (excitation/emmission wavelength), standardized to 20°C, Q10 factor of 2 (Winkler et al. 1996)
9	Phenol oxidase activity	Phen ox	µmol/h/ml	Schreckinger, José	L-DOPA, 200 fluorometer (Tecan, Männedorf, Switzerland), 365/460 nm (excitation/emmission wavelength), standardized to 20°C, Q10 factor of 2 (Winkler et al. 1996)
10	Peroxidase activity	Perox	µmol/h/ml	Schreckinger, José	L-DOPA, 200 fluorometer (Tecan, Männedorf, Switzerland), 365/460 nm (excitation/emmission wavelength), standardized to 20°C, Q10 factor of 2 (Winkler et al. 1996)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

297 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Date/Time	2 Time [days]	3 Treat	4 B-glu [µmol/h/ml]	5 Xylo [µmol/h/ml]	6 Chit [µmol/h/ml]	7 Phos [µmol/h/ml]	8 Leu aminopep [µmol/h/ml]	9 Phen ox [µmol/h/ml]	10 Perox [µmol/h/ml]
2018-10-22T10:00:00	0	dried	0.034677148	0.000000000	0.000000000	1.339639591	1.313547360	2.892083608	2.156798316
2018-10-22T10:00:00	0	dried	0.000000000	0.000000000	0.000000000	2.284369704	1.339433437	2.130633030	3.061452166
2018-10-22T10:00:00	0	dried	0.097065230	0.000000000	0.000000000	1.520588978	0.669380635	3.174816987	3.011613737
2018-10-22T10:00:00	0	migrated	0.000000000	0.000000000	0.000000000	9.076713687	2.185800385	0.514781915	2.429396449
2018-10-22T10:00:00	0	migrated	0.000000000	0.000000000	0.016005561	1.894900153	1.865634683	1.272412194	2.098752341
2018-10-22T10:00:00	0	migrated	0.000000000	0.000000000	0.000000000	2.694232704	0.876410612	0.000000000	2.223143572
2018-10-22T10:00:00	0	in situ	0.147432916	0.000000000	0.255487241	26.210841010	8.034047761	2.819460079	4.489228545
2018-10-22T10:00:00	0	in situ	0.000000000	0.000000000	0.148421935	16.501579120	4.791941271	2.079833200	3.886360255
2018-10-22T10:00:00	0	in situ	0.080111367	0.000000000	0.069669360	21.124912370	5.192799142	1.835388841	2.577274139
2018-11-12T10:00:00	21	dried	0.120827523	0.000000000	0.013154058	9.929871048	3.345099101	2.187778659	1.648480665
2018-11-12T10:00:00	21	dried	0.110788986	0.000000000	0.061690180	10.884480720	3.709819907	2.632180871	3.774662969
2018-11-12T10:00:00	21	dried	0.206768296	0.000000000	0.261928297	19.096608540	5.370682336	2.594408999	3.099964987
2018-11-12T10:00:00	21	migrated	0.190767321	0.000000000	0.108227701	10.830184110	4.962835795	2.955640523	2.805517019
2018-11-12T10:00:00	21	migrated	0.000000000	0.000000000	0.005407120	10.223357150	1.949033928	1.495654151	2.265030271
2018-11-12T10:00:00	21	migrated	0.000000000	0.000000000	0.233566891	14.505554870	1.884091773	4.361693439	3.887345227
2018-11-12T10:00:00	21	in situ	0.393995846	0.000000000	0.324311804	41.769886240	6.594578396	2.182273922	3.064651309
2018-11-12T10:00:00	21	in situ	0.441128036	0.006114370	0.430183594	32.056578950	6.214928120	1.956999531	2.113887265
2018-11-12T10:00:00	21	in situ	0.710077265	0.028320231	0.439629416	54.013398040	11.296566080	0.000000000	3.469367414
2018-11-12T10:00:00	21	control	0.188263307	0.000000000	0.410998141	38.594504390	8.976599678	2.186528587	2.790476590
2018-11-12T10:00:00	21	control	0.583315537	0.000000000	0.330750143	46.495938150	10.204014980	3.464953824	4.141630397
2018-11-12T10:00:00	21	control	0.000000000	0.000000000	0.137611310	25.958567580	4.041902403	3.244463352	2.234982395
2019-02-04T10:00:00	105	dried	1.030764868	0.000000000	0.567798717	44.405726550	14.619931830	5.990555905	6.040043800
2019-02-04T10:00:00	105	dried	0.308793553	0.000000000	0.376960430	11.099586260	4.911639148	4.586206144	3.099551321
2019-02-04T10:00:00	105	dried	0.298596928	0.021940348	0.318775643	8.578195697	3.636853387	2.897619174	3.858328279
2019-02-04T10:00:00	105	migrated	0.746155258	0.000000000	0.834409191	35.417343380	13.196824250	5.510372225	5.323101295
2019-02-04T10:00:00	105	migrated	0.136166507	0.000000000	0.080993993	15.253528200	0.000000000	2.293295085	2.765204092
2019-02-04T10:00:00	105	migrated	0.189580010	0.000000000	0.063018043	17.395258740	4.094076008	2.005705658	2.267668189
2019-02-04T10:00:00	105	in situ	1.980150052	0.474975872	0.977729401	99.345794760	33.186248360	5.111314986	3.654473282
2019-02-04T10:00:00	105	in situ	2.265470434	0.092930639	0.310264731	43.566844750	21.730251440	4.463124195	5.120583609
2019-02-04T10:00:00	105	in situ	3.543578768	0.101891340	1.443167854	98.842541440	45.481734010	4.846111847	4.488369824
2019-02-04T10:00:00	105	control	0.356534173	0.000000000	0.126574942	23.696776480	7.399808260	3.816312137	4.992454040
2019-02-04T10:00:00	105	control	0.969055125	0.000000000	0.363271850	30.710969760	21.703694580	4.900517248	7.743182458
2019-02-04T10:00:00	105	control	0.949695300	0.000000000	1.961416399	27.029692910	14.286473550	5.661240233	5.903984378