Oprei, Anna; Schreckinger, José; Kholiavko, Tatiana; Frossard, Aline; Mutz, Michael; Risse-Buhl, Ute (2023): Community abundance after drying and bedform migration in the Spree River in 2018/2019 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.941189, In: Oprei, A et al. (2023): Long-term recovery of metabolism, enzyme activity and community abundance after drying and bedform migration in the Spree river 2018/2019 [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.941237
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Abstract:
Sandy streambed sediments that had been exposed to either intense drying (90-day without rainfall) or bedform migration (periodically moving ripples) were placed in 250 µm mesh bags to the Spree River in Northeastern Germany on October 22, 2018, to initiate the recovery process from drying or migration stress. Subsamples from the recovering sediments were collected at eight dates within eight months and taken to the laboratory for further analysis. Community abundance was assessed on a heterotrophic (amplified bacterial and fungal gene copies) and on an autotrophic level (Chlorophyll a concentration and diatom cell count). The aim of the experiment was to observe the long-term recovery of microbial communities in lowland streams from drying and bedform migration and to identify if stress effects persisted as legacy after the stress period.
Keyword(s):
Supplement to:
Oprei, Anna; Schreckinger, José; Kholiavko, Tatiana; Frossard, Aline; Mutz, Michael; Risse-Buhl, Ute (2023): Long-term functional recovery and associated microbial community structure after sediment drying and bedform migration. Frontiers in Ecology and Evolution, 11, 1112519, https://doi.org/10.3389/fevo.2023.1112519
Related to:
Mendoza-Lera, Clara; Frossard, Aline; Knie, Matthias; Federlein, Laura L; Gessner, Mark O; Mutz, Michael (2017): Importance of advective mass transfer and sediment surface area for streambed microbial communities. Freshwater Biology, 62(1), 133-145, https://doi.org/10.1111/fwb.12856
Perez-Mon, Carla; Frey, Beat; Frossard, Aline (2020): Functional and Structural Responses of Arctic and Alpine Soil Prokaryotic and Fungal Communities Under Freeze-Thaw Cycles of Different Frequencies. Frontiers in Microbiology, 11, https://doi.org/10.3389/fmicb.2020.00982
Project(s):
Funding:
Coverage:
Latitude: 51.833333 * Longitude: 14.333333
Date/Time Start: 2018-10-22T10:00:00 * Date/Time End: 2019-06-03T10:00:00
Minimum Elevation: 60.0 m * Maximum Elevation: 60.0 m
Event(s):
Spree_2018-2019 * Latitude: 51.833333 * Longitude: 14.333333 * Date/Time Start: 2018-10-22T10:00:00 * Date/Time End: 2019-06-03T10:00:00 * Elevation: 60.0 m * Location: Spree River near Döbbrick, Brandenburg, Germany * Method/Device: Experiment (EXP) * Comment: Exposition and collection site of sediment samples
Parameter(s):
# | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
---|---|---|---|---|---|---|
1 | DATE/TIME | Date/Time | Oprei, Anna | Geocode – Collection date of sediment samples | ||
2 | Time in days | Time | days | Oprei, Anna | Days after start of recovery | |
3 | Treatment | Treat | Oprei, Anna | |||
4 | Chlorophyll a per unit sediment mass | Chl a/sed | µg/g | Risse-Buhl, Ute | Reverse phase HPLC (High Performance Liquid Chromatography) using Ultimate3000 (Thermo Fisher Scientific Corporation, Waltham, MA, USA) | Dry weight |
5 | Prokaryotic abundance, 16S gene copy number per unit sediment dry mass | Prok 16S gene/sed dm | #/g | Oprei, Anna | qPCR (primer pairs 27F/519r) with SYBR™ Green in ABI7500 machine (Applied Biosystems, Foster City, USA), Perez-Mon et al. (2020) | |
6 | Fungal abundance, ITS2 copy number per unit sediment dry mass | Fung ITS2/sed dm | #/g | Oprei, Anna | qPCR (primer pairs ITS3/ITS4) with SYBR™ Green in ABI7500 machine (Applied Biosystems, Foster City, USA), Perez-Mon et al. (2020) | |
7 | Diatoms | Diatoms | #/g | Risse-Buhl, Ute | Counting 400 living cells in Utermöhl chamber at 400x magnification, inverse light microscope (Axioskop2, Zeiss, Germany), extraction following Mendoza-Lera et al. (2017) | Cells/g dry weight |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
485 data points