Hamer, Jorin; Matthiessen, Birte; Pulina, Silvia; Hattich, Giannina S I (2022): Long term experiment using a semi-continuous batch cycle system with the phytoplankton species Chaetoceros affinis and Emiliania huxleyi [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.940796, In: Hamer, J et al. (2022): Trait measurements and long-term experiment on maintenance of intraspecific diversity using genotypes of phytoplankton species Chaetoceros affinis and Emiliania huxleyi [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.940800
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Abstract:
Long term experiment using a semi-continuous batch cycle system with the phytoplankton species Chaetoceros affinis and Emiliania huxleyi each consisting of 9 genotypes (http://roscoff-culture-collection.org/; Emiliania huxleyi: GC22: RCC6383, C42: RCC6376, C41: RCC6375, C47: RCC4546, C96: RCC6381, C91: RCC6380, C35: RCC6374, C30: RCC6371, C48: RCC6377, Chaetoceros affinis: B63: RCC6347, B67: RCC6348, B68: RCC6349, B74: RCC6350). Cultivation of species separately in mono-cultures and together in mix-cultures at three different nutrient regimes (10N:1P, 20N:1P, and 30N:1P) with increasing nitrate concentration over 91 days. Nutrient concentrations were 8.60 ± 0.62, 19.08 ± 0.32, and 29.36 ± 0.47 µmol L−1 nitrate and 0.93 ± 0.09 phosphate. Silicate concentrations were aligned to reflect a 4:1 N:Si ratio. Transfer of part of the cells into bottles with new nutrients every 7 days at fixed batch cycle length and after 7,4, and 10 days in a recurring fashion at variable batch cycle length. Measurements of cell density and cell volume at the end of every batch cycle and cell density or fluorescence daily during the third batch cycle. Nitrate, phosphate, and silicate measurements in mix-cultures at variable batch cycle length at the end of the first (7 days), second (4 days), and third (10 days) batch cycle. Daily nutrient measurements in mix-cultures from day 4 to day 7 at batch cycle seven.
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Date/Time Start: 2019-05-27T00:00:00 * Date/Time End: 2019-08-19T00:00:00
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experiment | Exp | Hamer, Jorin | type | ||
| 2 | DATE/TIME | Date/Time | Hamer, Jorin | Geocode – of experiment | ||
| 3 | Experiment day | Exp day | day | Hamer, Jorin | of batch cycle | |
| 4 | Number of batch | Batch no | # | Hamer, Jorin | batch cycle | |
| 5 | Bottle number | Bottle | # | Hamer, Jorin | Bottles 4,47,56, and 58 were excluded due to contamination | |
| 6 | Treatment: nutrients | T:nutrients | Hamer, Jorin | Nutrient regime | ||
| 7 | Treatment | Treat | Hamer, Jorin | Batch cycle length | ||
| 8 | Treatment | Treat | Hamer, Jorin | Culture | ||
| 9 | Emiliania huxleyi | E. huxleyi | #/ml | Hamer, Jorin | Microscopy (Zeiss Axiovert 200 and Axio Observer A1) | |
| 10 | Chaetoceros affinis | C. affinis | #/ml | Hamer, Jorin | Microscopy (Zeiss Axiovert 200 and Axio Observer A1) | |
| 11 | Fluorescence | Fluorescence | Hamer, Jorin | Fluorometer, Turner Designs, 10-AU | ||
| 12 | Emiliania huxleyi, cell biovolume | E. huxleyi cell biovol | µm3 | Hamer, Jorin | Microscopy (Zeiss Axiovert 200 and Axio Observer A1) | |
| 13 | Chaetoceros affinis, cell biovolume | C. affinis cell biovol | µm3 | Hamer, Jorin | Microscopy (Zeiss Axiovert 200 and Axio Observer A1) | |
| 14 | Silicate | Si(OH)4 | µmol/l | Hamer, Jorin | Autoanalyzer (Thermo Scientific Flash) | |
| 15 | Phosphate | [PO4]3- | µmol/l | Hamer, Jorin | Autoanalyzer (Thermo Scientific Flash) | |
| 16 | Nitrate | [NO3]- | µmol/l | Hamer, Jorin | Autoanalyzer (Thermo Scientific Flash) |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
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Curation Level: Enhanced curation (CurationLevelC)
Size:
18078 data points
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