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Leibold, Sandra; Lakshminarasimha, Amrutha Bagivalu; Gremse, Felix; Hammerschmidt, Matthias; Michel, Maximilian (2022): Micro CT scans for zebrafish on an obesogenic diet (DIO), on caloric restriction (CR) or undergoing catch up growth after 1, 3 or 9 months of CR (CG1, CG3, CG9) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.940201

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Abstract:
For µCT imaging, adult zebrafish were fixed and decalcified in Bouin's solution at room temperature for 7 days, stored in PBS and imaged using a micro-computed tomography (µCT) device (SkyScan1272, Bruker BioSpin GmbH, Ettlingen, Germany). Zebrafish were placed individually in 1.5ml Eppendorf tubes using and an ultra-focus scan over the whole body was performed in a full-rotation in step-and-shoot mode. 322 projections (1008x672 pixels, 4x4 binning) were acquired per subscan with an x-ray tube voltage of 60 kV, power 0.166 mA, aluminum filter 0.25 mm,exposure time of 363 ms, 6 averages and a object-source distance of 86 mm. All CT images were reconstructed at an isotropic voxel size of 18 µm using a Feldkamp type algorithm (filtered back-projection). Fat-containing regions were appear hypo intense in µCT data and were segmented using Imalytics Preclinical (Gremse-IT GmbH, Aachen, Germany (Gremse et al., 2016; doi:10.7150/thno.13624). The volumetric fat percentage was calculated as the ratio of subcutaneous adipose tissue (SAT) or visceral adipose tissue (VAT) fat volume compared to the entire volume of the body cavity anterior of the anal fin and expressed per skeletal segment.
Keyword(s):
Caloric Restriction; Diet Induced Obesity; Fish growth; Zebrafish; Zoology
Supplement to:
Leibold, Sandra; Lakshminarasimha, Amrutha Bagivalu; Gremse, Felix; Hammerschmidt, Matthias; Michel, Maximilian (accepted): Long-term obesogenic diet leads to metabolic phenotypes which are not exacerbated by catch-up growth in zebrafish. PLoS ONE
Related to:
Leibold, Sandra; Lakshminarasimha, Amrutha Bagivalu; Gremse, Felix; Hammerschmidt, Matthias; Michel, Maximilian (2022): Long-term obesogenic diet leads to metabolic phenotypes which are not exacerbated by catch-up growth in zebrafish. PANGAEA, https://doi.org/10.1594/PANGAEA.941313
Further details:
Dogan, Sukru Anil; Pujol, Claire; Maiti, Priyanka; Kukat, Alexandra; Wang, Shuaiyu; Hermans, Steffen; Senft, Katharina; Wibom, Rolf; Rugarli, Elena I; Trifunovic, Aleksandra (2014): Tissue-Specific Loss of DARS2 Activates Stress Responses Independently of Respiratory Chain Deficiency in the Heart. Cell Metabolism, 19(3), 458-469, https://doi.org/10.1016/j.cmet.2014.02.004
Eames, Stefani C; Philipson, Louis H; Prince, Victoria E; Kinkel, Mary D (2010): Blood Sugar Measurement in Zebrafish Reveals Dynamics of Glucose Homeostasis. Zebrafish, 7(2), 205-213, https://doi.org/10.1089/zeb.2009.0640
Gremse, Felix; Stärk, Marius; Ehling, Josef; Menzel, Jan Robert; Lammers, Twan; Kiessling, Fabian (2016): Imalytics Preclinical: Interactive Analysis of Biomedical Volume Data. Theranostics, 6(3), 328-341, https://doi.org/10.7150/thno.13624
Hopkins, Kevin D (1992): Reporting Fish Growth: A Review of the Basics. Journal of the World Aquaculture Society, 23(3), 173-179, https://doi.org/10.1111/j.1749-7345.1992.tb00766.x
Leibold, Sandra; Hammerschmidt, Matthias; Rawls, John F (2015): Long-Term Hyperphagia and Caloric Restriction Caused by Low- or High-Density Husbandry Have Differential Effects on Zebrafish Postembryonic Development, Somatic Growth, Fat Accumulation and Reproduction. PLoS ONE, 10(3), e0120776, https://doi.org/10.1371/journal.pone.0120776
Norin, Tommy; Malte, Hans (2011): Repeatability of standard metabolic rate, active metabolic rate and aerobic scope in young brown trout during a period of moderate food availability. Journal of Experimental Biology, 214(10), 1668-1675, https://doi.org/10.1242/jeb.054205
Steffensen, John Fleng (1989): Some errors in respirometry of aquatic breathers: How to avoid and correct for them. Fish Physiology and Biochemistry, 6(1), 49-59, https://doi.org/10.1007/BF02995809
Comment:
Fish were raised as previously reported (Leibold and Hammerschmidt, 2015) for the following conditions:
CG1: compensatory or catch up growth shifted at 1 month of age
CG3: compensatory or catch up growth shifted at 3 months of age
CG9: compensatory or catch up growth shifted at 9 months of age
CR: caloric restriction
DIO: diet induced obesity
The CT .nii files correlate to the groups as follows: Group 2: CG1; Group 3: DIO1; Group 6: CG3; Group 7 DIO3; Group 10: CG9; Group 11: DIO9; Group 1: CR1; Group 5: CR3; Group 9: CR9
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 Binary ObjectBinaryMichel, Maximilian
2 Binary Object (MD5 Hash)Binary (Hash)Michel, Maximilian
3 Binary Object (Media Type)Binary (Type)Michel, Maximilian
4 Binary Object (File Size)Binary (Size)BytesMichel, Maximilian
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Basic curation (CurationLevelB)
Size:
36 data points

Data

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1 
Binary		2 
Binary (Hash)		3 
Binary (Type)		4 
Binary (Size) [Bytes]
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