Citation:

PANGAEA.939902

Name: Seawater carbonate chemistry and phytoplankton growth and mortality
Published: 2021
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Community composition and diversity; Entire community; Growth/Morphology; Laboratory experiment; Mediterranean Sea; Other metabolic rates; Pelagos; Primary production/Photosynthesis; Temperate; Temperature

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Abstract:

Changes in temperature and CO2 are typically associated with climate change, but they also act on shorter time scales, leading to alterations in phytoplankton physiology and community structure. Interactions among stressors may cause synergistic or antagonistic effects on phytoplankton dynamics. Therefore, the main goal of this work is to understand the short-term isolated and interactive effects of warming and high CO2 on phytoplankton nutrient consumption, growth, production, and community structure in the Ria Formosa coastal lagoon (southern Portugal). We performed microcosm experiments with temperature and CO2 manipulation, and dilution experiments under temperature increase, using winter phytoplankton assemblages. Phytoplankton responses were evaluated using inverted and epifluorescence microscopy. Overall, phytoplankton growth and microzooplankton grazing on phytoplankton decreased with warming. Negative antagonist interactions with CO2 alleviated the negative effect of temperature on phytoplankton and cryptophytes. In contrast, higher temperature benefited smaller-sized phytoplankton, namely cyanobacteria and eukaryotic picophytoplankton. Diatom growth was not affected by temperature, probably due to nutrient limitation, but high CO2 had a positive effect on diatoms, alleviating the effect of nutrient limitation. Results suggest that this winter phytoplankton assemblage is well acclimated to ambient conditions, and short-term increases in temperature are detrimental, but can be alleviated by high CO2.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Community composition and diversity; Entire community; Growth/Morphology; Laboratory experiment; Mediterranean Sea; Other metabolic rates; Pelagos; Primary production/Photosynthesis; Temperate; Temperature

Supplement to:

Domingues, Rita B; Barreto, Marcelle; Brotas, Vanda; Galvão, Helena M; Barbosa, Ana B (2021): Short-term effects of winter warming and acidification on phytoplankton growth and mortality: more losers than winners in a temperate coastal lagoon. Hydrobiologia, 848(20), 4763-4785, https://doi.org/10.1007/s10750-021-04672-0

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-1-6.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Domingues, Rita B		study
2	Treatment	Treat		Domingues, Rita B
3	Nitrate consumption rate	[NO3]- con rate	µmol/l/day	Domingues, Rita B		net
4	Nitrate consumption rate, standard error	[NO3]- con rate std e	±	Domingues, Rita B		net
5	Ammonium consumption rate	[NH4]+ con rate	µmol/l/day	Domingues, Rita B		net
6	Ammonium consumption rate, standard error	[NH4]+ con rate std e	±	Domingues, Rita B		net
7	Phosphate consumption rate	[PO4]3- con rate	µmol/l/day	Domingues, Rita B		net
8	Phosphate consumption rate, standard error	[PO4]3- con rate std e	±	Domingues, Rita B		net
9	Silicate consumption rate	Si(OH)4 con rate	µmol/l/day	Domingues, Rita B		net
10	Silicate consumption rate, standard error	Si(OH)4 con rate std e	±	Domingues, Rita B		net
11	Growth rate	µ	1/day	Domingues, Rita B		phytoplankton community
12	Growth rate, standard error	µ std e	±	Domingues, Rita B		phytoplankton community
13	Growth rate	µ	1/day	Domingues, Rita B		diatoms
14	Growth rate, standard error	µ std e	±	Domingues, Rita B		diatoms
15	Growth rate	µ	1/day	Domingues, Rita B		cryptophytes
16	Growth rate, standard error	µ std e	±	Domingues, Rita B		cryptophytes
17	Growth rate	µ	1/day	Domingues, Rita B		cyanobacteria
18	Growth rate, standard error	µ std e	±	Domingues, Rita B		cyanobacteria
19	Irradiance	E	µmol/m²/s	Domingues, Rita B
20	Specific primary production of carbon per Chlorophyll a	PP spec/Chl a	mg/mg/h	Domingues, Rita B
21	Production rate, maximal, light saturated, as carbon per chlorophyll a	PBmax	mg C/mg Chl a/h	Domingues, Rita B
22	Production rate, maximal, light saturated, as carbon normalized to chlorophyll a, standard error	PBmax std e	±	Domingues, Rita B
23	Light saturation point	Ik	µmol/m²/s	Domingues, Rita B
24	Light saturation point, standard error	Ik std e	±	Domingues, Rita B
25	Photosynthetic efficiency, carbon production	alpha	mg C/(µE/m²/s)/mg Chl/h	Domingues, Rita B
26	Photosynthetic efficiency, standard error	alpha std e	±	Domingues, Rita B
27	Salinity	Sal		Domingues, Rita B
28	Temperature, water	Temp	°C	Domingues, Rita B
29	pH	pH		Domingues, Rita B		total scale
30	Alkalinity, total	AT	µmol/kg	Domingues, Rita B
31	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1219 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Treat	3 [NO3]- con rate [µmol/l/day] (net)	4 [NO3]- con rate std e [±] (net)	5 [NH4]+ con rate [µmol/l/day] (net)	6 [NH4]+ con rate std e [±] (net)	7 [PO4]3- con rate [µmol/l/day] (net)	8 [PO4]3- con rate std e [±] (net)	9 Si(OH)4 con rate [µmol/l/day] (net)	10 Si(OH)4 con rate std e [±] (net)	11 µ [1/day] (phytoplankton community)	12 µ std e [±] (phytoplankton community)	13 µ [1/day] (diatoms)	14 µ std e [±] (diatoms)	15 µ [1/day] (cryptophytes)	16 µ std e [±] (cryptophytes)	17 µ [1/day] (cyanobacteria)	18 µ std e [±] (cyanobacteria)	19 E [µmol/m²/s]	20 PP spec/Chl a [mg/mg/h]	21 PBmax [mg C/mg Chl a/h]	22 PBmax std e [±]	23 Ik [µmol/m²/s]	24 Ik std e [±]	25 alpha [mg C/(µE/m²/s)/mg Chl/h]	26 alpha std e [±]	27 Sal	28 Temp [°C]	29 pH (total scale)	30 AT [µmol/kg]	31 CSC flag	32 CO2 [µmol/kg]	33 fCO2water_SST_wet [µatm]	34 pCO2water_SST_wet [µatm]	35 [HCO3]- [µmol/kg]	36 [CO3]2- [µmol/kg]	37 DIC [µmol/kg]	38 Omega Arg	39 Omega Cal
laboratory	ambient	0.291	0.069	-0.076	0.031	0.065	0.016	-0.551	0.28020	0.3099	0.14	-0.39170	0.20	-0.08165	0.07067	-1.167	0.3382			2.47	0.10	322	25	0.00574	0.00123	35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	high CO2	0.204	0.065	-0.148	0.036	0.068	0.012	2.239	0.37330	0.1910	0.16	-0.02667	0.14	-0.30120	0.08330	-1.167	0.1912			2.54	0.17	336	69	0.00817	0.00300	35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high T	0.254	0.029	0.050	0.021	0.019	0.011	1.225	0.09244	-0.4654	0.23	-0.61000	0.12	-1.12700	0.29470	-0.425	0.1601			2.91	0.38	401	303	0.02190	0.00964	35	16.23	8.10	2636.99	8	14.56	403.15	404.59	2137.05	207.08	2358.68	3.18	4.94
laboratory	high T + high CO2	0.254	0.028	0.067	0.003	0.020	0.013	1.225	0.09244	-0.1368	0.09	-0.56000	0.07	-0.52680	0.11190	-0.450	0.1118			1.95	0.15	408	110	0.00685	0.00179	35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87
laboratory	ambient																	0	0.07							35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	ambient																	0	0.11							35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	ambient																	0	0.06							35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	ambient																	0	0.08							35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	ambient																	47	0.33							35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	ambient																	47	0.38							35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	ambient																	47	0.44							35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	ambient																	113	0.91							35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	ambient																	113	0.87							35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	ambient																	113	0.96							35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	ambient																	230	2.02							35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	ambient																	230	1.92							35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	ambient																	230	2.51							35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	ambient																	365	2.22							35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	ambient																	365	2.21							35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	ambient																	365	2.81							35	17.45	8.14	2683.65	8	12.54	359.15	360.41	2109.48	238.80	2360.82	3.68	5.70
laboratory	high CO2																	0	0.07							35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high CO2																	0	0.11							35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high CO2																	0	0.06							35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high CO2																	0	0.08							35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high CO2																	47	0.40							35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high CO2																	47	0.55							35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high CO2																	47	0.54							35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high CO2																	113	0.87							35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high CO2																	113	1.35							35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high CO2																	113	1.10							35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high CO2																	230	1.67							35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high CO2																	230	2.65							35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high CO2																	230	2.45							35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high CO2																	365	1.85							35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high CO2																	365	3.16							35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high CO2																	365	2.55							35	16.45	7.92	2702.84	8	23.82	664.08	666.44	2337.85	151.86	2513.53	2.33	3.62
laboratory	high T																	0	0.07							35	16.23	8.10	2636.99	8	14.56	403.15	404.59	2137.05	207.08	2358.68	3.18	4.94
laboratory	high T																	0	0.11							35	16.23	8.10	2636.99	8	14.56	403.15	404.59	2137.05	207.08	2358.68	3.18	4.94
laboratory	high T																	0	0.06							35	16.23	8.10	2636.99	8	14.56	403.15	404.59	2137.05	207.08	2358.68	3.18	4.94
laboratory	high T																	0	0.08							35	16.23	8.10	2636.99	8	14.56	403.15	404.59	2137.05	207.08	2358.68	3.18	4.94
laboratory	high T																	47	0.55							35	16.23	8.10	2636.99	8	14.56	403.15	404.59	2137.05	207.08	2358.68	3.18	4.94
laboratory	high T																	47	0.59							35	16.23	8.10	2636.99	8	14.56	403.15	404.59	2137.05	207.08	2358.68	3.18	4.94
laboratory	high T																	47	1.08							35	16.23	8.10	2636.99	8	14.56	403.15	404.59	2137.05	207.08	2358.68	3.18	4.94
laboratory	high T																	113	1.37							35	16.23	8.10	2636.99	8	14.56	403.15	404.59	2137.05	207.08	2358.68	3.18	4.94
laboratory	high T																	113	1.82							35	16.23	8.10	2636.99	8	14.56	403.15	404.59	2137.05	207.08	2358.68	3.18	4.94
laboratory	high T																	113	2.84							35	16.23	8.10	2636.99	8	14.56	403.15	404.59	2137.05	207.08	2358.68	3.18	4.94
laboratory	high T																	230	2.14							35	16.23	8.10	2636.99	8	14.56	403.15	404.59	2137.05	207.08	2358.68	3.18	4.94
laboratory	high T																	230	2.74							35	16.23	8.10	2636.99	8	14.56	403.15	404.59	2137.05	207.08	2358.68	3.18	4.94
laboratory	high T																	230	4.92							35	16.23	8.10	2636.99	8	14.56	403.15	404.59	2137.05	207.08	2358.68	3.18	4.94
laboratory	high T																	365	2.75							35	16.23	8.10	2636.99	8	14.56	403.15	404.59	2137.05	207.08	2358.68	3.18	4.94
laboratory	high T																	365	3.18							35	16.23	8.10	2636.99	8	14.56	403.15	404.59	2137.05	207.08	2358.68	3.18	4.94
laboratory	high T + high CO2																	0	0.07							35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87
laboratory	high T + high CO2																	0	0.11							35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87
laboratory	high T + high CO2																	0	0.06							35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87
laboratory	high T + high CO2																	0	0.08							35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87
laboratory	high T + high CO2																	47	0.36							35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87
laboratory	high T + high CO2																	47	0.39							35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87
laboratory	high T + high CO2																	47	0.33							35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87
laboratory	high T + high CO2																	113	0.84							35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87
laboratory	high T + high CO2																	113	0.86							35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87
laboratory	high T + high CO2																	113	0.79							35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87
laboratory	high T + high CO2																	230	1.44							35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87
laboratory	high T + high CO2																	230	1.85							35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87
laboratory	high T + high CO2																	230	1.48							35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87
laboratory	high T + high CO2																	365	1.72							35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87
laboratory	high T + high CO2																	365	2.32							35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87
laboratory	high T + high CO2																	365	1.77							35	14.70	7.85	2616.88	8	28.98	766.57	769.36	2325.38	120.37	2474.73	1.84	2.87