Citation:

PANGAEA.939724

Name: Nitrous oxide production kinetics from ammonia oxidation in the Eastern Tropical North Pacific in March/April 2018
Published: 2021-12-20
License: https://creativecommons.org/licenses/by/4.0/
Keywords: 15N-tracer; ammonium oxidation; eastern tropical north pacific; greenhouse gas; N2O production rates; nitrogen cycle; ocean

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Published: 2021-12-20 • DOI registered: 2023-01-10

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Abstract:

In March/April 2018 during a cruise on R/V Sally Ride, SR1805, 15N-NH4+ incubations in 60mL glass serum bottles were performed to measure ammonium oxidation rates to nitrite and nitrous oxide in different depth at 3 different stations in the oxygen deficient zone (ODZ) of the Eastern Tropical North Pacific off the coast of Mexico. Water samples were collected from 30L Niskin bottles deployed with a conductivity-temperature-depth profiler (CTD, Seabird Electronics). The goal was to get a better understanding on the controls of nitrous oxide (N2O) production. The N2O production rate experiments were performed according to Bourbonnais et al. 2021 (https://doi.org/10.3389/fmars.2021.611937). Furthermore, ammonium (NH4+), nitrite (NO2-) and nitrate (NO3-) as well as N2O concentrations were determined using standard fluorometric (Holmes et al. 1999, https://doi.org/10.1139/f99-128), photometric (Strickland and Parsons 1972, hdl:10013/epic.46454.d001), chemiluminescent (Braman and Hendrix 1989, doi:10.1021/ac00199a007) and mass spectrometric techniques (McIlvin and Casciotti 2010, https://doi.org/10.4319/lom.2010.8.54), respectively. The N2O yield per nitrite produced was calculated. The archaeal ammonia monooxygenase gene subunit A (amoA) copy numbers/mL were determined using qPCR as described previously (Peng et al. 2015, https://doi.org/10.1002/2015GB005278).

Keyword(s):

15N-tracer; ammonium oxidation; eastern tropical north pacific; greenhouse gas; N2O production rates; nitrogen cycle; ocean

Supplement to:

Frey, Claudia; Sun, Xin; Szemberski, Laura; Casciotti, Karen L; Garcia-Robledo, Emilio; Jayakumar, Amal; Kelly, C L; Lehmann, Moritz F; Ward, Bess B (2022): Kinetics of nitrous oxide production from ammonia oxidation in the Eastern Tropical North Pacific. Limnology and Oceanography, https://doi.org/10.1002/lno.12283

Related to:

Bourbonnais, Annie; Frey, Claudia; Sun, Xin; Bristow, Laura Anne; Jayakumar, Amal; Ostrom, Nathaniel E; Casciotti, Karen L; Ward, Bess B (2021): Protocols for Assessing Transformation Rates of Nitrous Oxide in the Water Column. Frontiers in Marine Science, 8, https://doi.org/10.3389/fmars.2021.611937

Braman, Robert S; Hendrix, Steven A (1989): Nanogram nitrite and nitrate determination in environmental and biological materials by vanadium(III) reduction with chemiluminescence detection. Analytical Chemistry, 61(24), 2715-2718, https://doi.org/10.1021/ac00199a007

Holmes, Robert M; Aminot, A; Kerouel, Roger; Hooker, Bethanie A; Peterson, Bruce J (1999): A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Canadian Journal of Fisheries and Aquatic Sciences, 56(10), 1801-1808, https://doi.org/10.1139/f99-128

McIlvin, Matthew R; Casciotti, Karen L (2010): Fully automated system for stable isotopic analyses of dissolved nitrous oxide at natural abundance levels. Limnology and Oceanography-Methods, 8(2), 54-66, https://doi.org/10.4319/lom.2010.8.54

Peng, Xuefeng; Fuchsman, Clara A; Jayakumar, Amal; Oleynik, Sergey; Martens-Habbena, Willm; Devol, Allan H; Ward, Bess B (2015): Ammonia and nitrite oxidation in the Eastern Tropical North Pacific. Global Biogeochemical Cycles, 29(12), 2034-2049, https://doi.org/10.1002/2015GB005278

Strickland, John D H; Parsons, T R (1972): A practical handbook of seawater analysis. Fisheries Research Board of Canada, hdl:10013/epic.46454.d001

Ward, Bess B; Casciotti, Karen L (2021): Bottle data from CTD casts conducted on R/V Sally Ride cruise SR1805 in the Eastern Tropical North Pacific Ocean from March to April 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO), https://doi.org/10.26008/1912/bco-dmo.854091.1

Funding:

National Science Foundation (NSF), grant/award no. 1657663 : Collaborative Research: Mechanisms and Controls of Nitrous Oxide Production in the Eastern Tropical North Pacific Ocean

National Science Foundation (NSF), grant/award no. 1657868 : Collaborative Research: Mechanisms and Controls of Nitrous Oxide Production in the Eastern Tropical North Pacific Ocean

Coverage:

Median Latitude: 14.483349 * Median Longitude: -106.783299 * South-bound Latitude: 10.000020 * West-bound Longitude: -113.000020 * North-bound Latitude: 17.683370 * East-bound Longitude: -102.350000

Date/Time Start: 2018-03-19T15:28:17 * Date/Time End: 2018-04-08T15:16:35

Minimum DEPTH, water: 15.857 m * Maximum DEPTH, water: 1002.448 m

Event(s):

SR1805_PS1_CTD5 * Latitude: 10.000040 * Longitude: -112.999980 * Date/Time: 2018-03-19T15:28:17 * Elevation: -4101.0 m * Location: North Pacific Ocean * Campaign: * Basis: * Method/Device: (CTD-RO)

SR1805_PS1_CTD16 * Latitude: 10.000120 * Longitude: -113.000000 * Date/Time: 2018-03-21T08:07:45 * Elevation: -4101.0 m * Location: North Pacific Ocean * Campaign: * Basis: * Method/Device: (CTD-RO)

SR1805_PS2_CTD32 * Latitude: 15.766660 * Longitude: -104.999780 * Date/Time: 2018-03-27T17:27:40 * Elevation: -2998.0 m * Location: North Pacific Ocean * Campaign: * Basis: * Method/Device: (CTD-RO)

Comment:

The data table contains the depth profiles of salinity, temperature (°C), oxygen concentration (µmol/L), ammonium (µmol/L), nitrate (µmol/L), nitrite (µmol/L) and nitrous oxide concentration (nmol/L) for three stations in the oxygen deficient zones (ODZ) in the Eastern Tropical North Pacific. It also contains the rate measurements of N2O production rate (nmol N2O/L*d) with standard error, ammonium oxidation rate (nmol N2O/L*d) to nitrite with standard error as well as N2O yield (%) with standard deviation. The % hybrid N2O production refers to the production of 45N2O, which requires incorporation of a 14N substrate other than NH4+. It is expressed in percent with respect to the total N2O produced from 15NH4+. Furthermore, the archaeal amoA copy numbers / mL and the standard deviation are given for the different depths.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Frey, Claudia
2	DATE/TIME	Date/Time		Frey, Claudia		Geocode
3	Station label	Station		Frey, Claudia
4	Treatment	Treat		Frey, Claudia
5	Depth, bottom/max	Depth bot	m	Frey, Claudia
6	Identification	ID		Frey, Claudia		CTD
7	Cast number	Cast		Frey, Claudia
8	Bottle number	Bottle		Frey, Claudia		Niskin
9	LATITUDE	Latitude		Frey, Claudia		Geocode
10	LONGITUDE	Longitude		Frey, Claudia		Geocode
11	DEPTH, water	Depth water	m	Frey, Claudia		Geocode
12	Density, sigma-theta (0)	Sigma-theta	kg/m³	Frey, Claudia	CTD, Sea-Bird
13	Salinity	Sal		Frey, Claudia	CTD, Sea-Bird	psu
14	Temperature, water	Temp	°C	Frey, Claudia	CTD, Sea-Bird
15	Radiation, photosynthetically active	PAR		Frey, Claudia	CTD, Sea-Bird
16	Oxygen	O2	µmol/l	Frey, Claudia	CTD, Sea-Bird
17	Nitrite	[NO2]-	µmol/l	Frey, Claudia	Photometry (Strickland & Parsons, 1972)
18	Nitrate	[NO3]-	µmol/l	Frey, Claudia	Chemiluminescence detection (Braman and Hendrix 1989)
19	Ammonium	[NH4]+	nmol/l	Frey, Claudia	Fluorometry (Holmes et al. 1999)
20	Nitrous oxide, dissolved	N2O	nmol/l	Frey, Claudia	Mass spectrometry (McIlvin and Casciotti 2010)	average between duplicates
21	Nitrous oxide, standard deviation	N2O std dev	±	Frey, Claudia		std dev between duplicates
22	Oxygen	O2	µmol/l	Frey, Claudia	Switchable trace oxygen sensor (STOX)	ultra low concentrations
23	Nitrous oxide production	N2O prod	nmol/l/day	Frey, Claudia	15N tracer incubations (Bourbonnais et al. 2021)
24	Nitrous oxide production, standard error	N2O prod std e	±	Frey, Claudia
25	Comment	Comment		Frey, Claudia		Significance y/n (yes/no)
26	Ammonium, oxidation rate	[NH4]+ OR	nmol/l/day	Frey, Claudia	15N tracer incubations (Bourbonnais et al. 2021)
27	Ammonium, oxidation rate, standard error	[NH4]+OR std e	±	Frey, Claudia
28	Comment	Comment		Frey, Claudia		Significance y/n (yes/no)
29	Nitrous oxide, hybrid	N2O hybrid	%	Frey, Claudia	15N tracer incubations (Bourbonnais et al. 2021)
30	Nitrous oxide, hybrid, standard error	N2O hybrid std e	±	Frey, Claudia
31	Nitrous oxide, yield	N2O yield	%	Frey, Claudia	Calculated
32	Nitrous oxide, yield, standard error	N2O yield std e	±	Frey, Claudia	Calculated
33	Ammonium, labelled, fraction	[NH4]+ labelled frac		Frey, Claudia
34	amoA gene, copy number	amoA copies	#/ml	Frey, Claudia	Real-time quantitative polymerase chain reaction (qPCR)	average
35	amoA gene, copy number, standard deviation	amoA copies std dev	±	Frey, Claudia	Real-time quantitative polymerase chain reaction (qPCR)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

796 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 Date/Time	3 Station	4 Treat	5 Depth bot [m]	6 ID (CTD)	7 Cast	8 Bottle (Niskin)	9 Latitude	10 Longitude	11 Depth water [m]	12 Sigma-theta [kg/m³] (CTD, Sea-Bird)	13 Sal (psu, CTD, Sea-Bird)	14 Temp [°C] (CTD, Sea-Bird)	15 PAR (CTD, Sea-Bird)	16 O2 [µmol/l] (CTD, Sea-Bird)	17 [NO2]- [µmol/l] (Photometry (Strickland & Pars...)	18 [NO3]- [µmol/l] (Chemiluminescence detection (...)	19 [NH4]+ [nmol/l] (Fluorometry (Holmes et al. 1999))	20 N2O [nmol/l] (average between duplicates, M...)	21 N2O std dev [±] (std dev between duplicates)	22 O2 [µmol/l] (ultra low concentrations, Swi...)	23 N2O prod [nmol/l/day] (15N tracer incubations (Bourb...)	24 N2O prod std e [±]	25 Comment (Significance y/n (yes/no))	26 [NH4]+ OR [nmol/l/day] (15N tracer incubations (Bourb...)	27 [NH4]+OR std e [±]	28 Comment (Significance y/n (yes/no))	29 N2O hybrid [%] (15N tracer incubations (Bourb...)	30 N2O hybrid std e [±]	31 N2O yield [%] (Calculated)	32 N2O yield std e [±] (Calculated)	33 [NH4]+ labelled frac	34 amoA copies [#/ml] (average, Real-time quantitati...)	35 amoA copies std dev [±] (Real-time quantitative polyme...)
SR1805_PS1_CTD5	2018-03-19T15:28:17	outside ODZ PS1	depth profile	4101	CTD5	5	11	10.00004	-112.99998	30.865	21.4726	33.6784	27.7870	175.600000	199.106	0.00	0.38	0.00				0.00010	0.00000	n	0.00100	0.00010	y					0.99000	8.23000	2.15000
SR1805_PS1_CTD5	2018-03-19T15:28:17	outside ODZ PS1	depth profile	4101	CTD5	5	9	10.00002	-112.99998	60.556	23.3020	34.2313	23.1818	23.817000	106.122	1.52	8.66	8.75				0.00706	0.00151	y	0.83900	0.09140	y	98.53	10.17	0.42	0.10	0.97087	7793.98000	376.04000
SR1805_PS1_CTD5	2018-03-19T15:28:17	outside ODZ PS1	depth profile	4101	CTD5	5	7	10.00004	-112.99998	69.546	24.4278	34.5245	19.9032	9.149500	46.241	0.09	17.01	3.98			47.490	0.02831	0.00312	y	1.08000	0.08390	y	98.28	1.70	1.31	0.18	0.98121	30643.96000	4185.54000
SR1805_PS1_CTD5	2018-03-19T15:28:17	outside ODZ PS1	depth profile	4101	CTD5	5	4	10.00004	-112.99997	88.796	25.5199	34.7015	16.0054	1.223500	0.354	0.00	24.30	54.50			0.930	0.01477	0.00357	y	0.26000	0.02910	y	98.28	3.56	2.84	0.76	0.88183	11592.20000	1191.10000
SR1805_PS1_CTD16	2018-03-21T08:07:45	outside ODZ PS1	depth profile	4101	CTD16	16	10	10.00012	-113.00000	100.708	25.8429	34.7622	14.7662	1.335300	-0.619	0.02	23.79	3.70			0.260	0.01069	0.00194	y	0.30700	0.02390	y	98.06	3.47	1.74	0.34	0.98183	51991.57000	8038.40000
SR1805_PS1_CTD5	2018-03-19T15:28:17	outside ODZ PS1	depth profile	4101	CTD5	5	1	10.00006	-112.99996	109.029	26.0496	34.7812	13.8645	0.216140	-0.405	0.03	27.52	2.14			0.160	0.00004	0.00074	n	0.02800	0.00330	y					0.98525	16552.71000	3589.35000
SR1805_PS1_CTD16	2018-03-21T08:07:45	outside ODZ PS1	depth profile	4101	CTD16	16	8	10.00012	-113.00000	153.034	26.3754	34.7887	12.2781	0.000000	-0.289	0.00	27.97	6.66			0.500	-0.00027	0.00092	n	0.00100	0.00010	y					0.97538	6394.84000	425.12000
SR1805_PS1_CTD16	2018-03-21T08:07:45	outside ODZ PS1	depth profile	4101	CTD16	16	6	10.00010	-113.00001	262.826	26.6013	34.7257	10.8131	0.000000	1.606	0.08	27.89	3.65			3.000	-0.00003	0.00095	n	0.00100	0.00080	y					0.98193	9454.96000	2594.57000
SR1805_PS1_CTD16	2018-03-21T08:07:45	outside ODZ PS1	depth profile	4101	CTD16	16	4	10.00010	-113.00002	502.847	26.9707	34.5862	7.9046	0.000000	-1.038	0.01	28.79	3.78			0.040	0.00423	0.02270	n	0.00000	0.00000	y					0.98164	1825.57000	627.86000
SR1805_PS1_CTD16	2018-03-21T08:07:45	outside ODZ PS1	depth profile	4101	CTD16	16	1	10.00010	-113.00000	1002.448	27.4097	34.5611	4.3390	0.000000	28.757	0.02	38.23	5.09			29.510	0.00011	0.00143	n	0.00000	0.00000	y					0.97879	3767.28000	2295.76000
SR1805_PS2_CTD32	2018-03-27T17:27:40	OMZ PS2	depth profile	2998	CTD32	32	11	15.76666	-104.99978	60.100	23.6671	34.4284	22.4262	43.019000	194.030	0.62	0.00	6.61	91.02	13.00		-0.00030	0.00130	n	0.02929	0.00793	y					0.97548	53.12000	9.79000
SR1805_PS2_CTD32	2018-03-27T17:27:40	OMZ PS2	depth profile	2998	CTD32	32	9	15.76664	-104.99978	74.663	24.6834	34.4682	18.7418	8.487800	50.974	0.13	15.35	12.22	101.78	14.57	50.260	0.03000	0.01270	y	2.74762	0.93982	y	0.55	0.32	96.69	5.63	0.96349	905.07000	319.31000
SR1805_PS2_CTD32	2018-03-27T17:27:40	OMZ PS2	depth profile	2998	CTD32	32	6	15.76666	-104.99978	94.699	25.3722	34.6455	16.4614	1.667300	3.532	0.00	24.70	13.65	144.01	23.11	3.750	0.03180	0.01000	y	0.97888	0.08021	y	1.62	0.53	90.32	20.00	0.96038	14975.78000	1549.75000
SR1805_PS2_CTD32	2018-03-27T17:27:40	OMZ PS2	depth profile	2998	CTD32	32	4	15.76664	-104.99976	119.775	25.8604	34.7556	14.6646	0.231990	-0.733	0.03	23.30	50.04	48.59	6.63	0.050	-0.00010	0.00000	n	0.00929	0.00377	y					0.88979	790.21000	370.33000
SR1805_PS2_CTD32	2018-03-27T17:27:40	OMZ PS2	depth profile	2998	CTD32	32	1	15.76666	-104.99978	150.443	26.2095	34.7915	13.1295	0.040890	-0.764	1.36	22.56	2.13	7.38	0.97	0.060	0.00000	0.00000	n	0.00241	0.00123	n					0.98527	1087.47000	368.64000
SR1805_PS2_CTD45	2018-03-30T00:02:55	OMZ PS2	depth profile	3058	CTD45	45	9	15.76672	-104.99998	201.028	26.3938	34.7824	12.1639	0.025552	-1.559	1.39	22.83	2.05	4.54	0.58	0.040	0.00000	0.00080	n	0.00247	0.00061	y					0.98534	560.45793	185.07312
SR1805_PS2_CTD45	2018-03-30T00:02:55	OMZ PS2	depth profile	3058	CTD45	45	7	15.76670	-104.99998	250.941	26.5072	34.7394	11.3893	0.023113	-1.070	1.62	23.71	1.22	4.65	0.62	0.020	0.00010	0.00090	n	0.00022	0.00106	n					0.98689	50.62269	25.59859
SR1805_PS2_CTD45	2018-03-30T00:02:55	OMZ PS2	depth profile	3058	CTD45	45	5	15.76672	-105.00000	300.632	26.5952	34.7056	10.7642	0.022470	-1.057	1.37	24.03	2.00	4.89	0.51	0.000	0.00000	0.00110	n	0.00000	0.00036	y					0.98556	39.47983	19.16856
SR1805_PS2_CTD45	2018-03-30T00:02:55	OMZ PS2	depth profile	3058	CTD45	45	3	15.76669	-104.99998	501.053	26.9031	34.5824	8.3365	0.020348	-1.043	0.00	28.00	3.97	31.68	5.62	0.060	0.00010	0.00080	n	0.00053	0.00009	y					0.98116	1610.21984	1703.12717
SR1805_PS2_CTD45	2018-03-30T00:02:55	OMZ PS2	depth profile	3058	CTD45	45	1	15.76666	-105.00000	850.293	27.2924	34.5406	5.2316	0.018748	0.447	0.00	42.04	2.24	84.46	13.37	2.590	0.00000	0.00060	n	0.00024	0.00010	n					0.98358	335.31598	104.28749
SR1805_PS3_CTD71	2018-04-05T13:20:12	OMZ PS3 coastaL	depth profile	1030	CTD71	71	11	17.68334	-102.35004	15.857	23.8063	34.4772	22.0545	46.279000	124.281	0.40	5.22	399.62	110.40	7.86		0.04676	0.01519	y	0.22368	0.01662	n	95.18	7.12			0.52194	206.24927	43.69975
SR1805_PS3_CTD71	2018-04-05T13:20:12	OMZ PS3 coastaL	depth profile	1030	CTD71	71	9	17.68334	-102.35000	24.883	24.7389	34.5861	18.8704	9.230800	29.066	0.33	19.64	12.39	214.39	15.70	6.800	0.12876	0.01896	y	11.72723	0.32990	y	95.52	6.89	0.55	0.96	0.96313	1.62400	0.44433
SR1805_PS3_CTD71	2018-04-05T13:20:12	OMZ PS3 coastaL	depth profile	1030	CTD71	71	6	17.68337	-102.35000	34.850	25.0344	34.6330	17.8289	2.624100	7.203	0.26	23.81	9.31	208.14	21.99	1.060	0.14943	0.01504	y	6.38495	0.22128	y	97.08	3.22	1.17	0.12	0.96967	10835.03763	739.60907
SR1805_PS3_CTD71	2018-04-05T13:20:12	OMZ PS3 coastaL	depth profile	1030	CTD71	71	3	17.68336	-102.35002	44.609	25.3784	34.6931	16.5835	0.935480	-0.200	0.19	25.98	3.38	155.65	12.52	0.110	0.00822	0.00310	y	0.18608	0.32573	n	98.28	10.44			0.98251	9419.17704	107.70888
SR1805_PS3_CTD84	2018-04-08T15:16:35	OMZ PS3 coastaL	depth profile	1028	CTD84	84	11	17.68328	-102.35004	60.140	25.5037	34.7278	16.1604	0.708750	-0.119	0.60	25.52	15.85	67.65	9.20	0.060	0.01609	0.00176	y	0.18319	0.02789	y	98.07	2.64	4.39	0.58	0.95590	2181.74034	463.69765
SR1805_PS3_CTD84	2018-04-08T15:16:35	OMZ PS3 coastaL	depth profile	1028	CTD84	84	9	17.68323	-102.35004	70.215	25.7186	34.7669	15.3440	0.277980	-1.327		21.78	4.87	19.71	2.13	0.005	0.01956	0.00229	y	0.00000	0.16767	n	97.50	5.98			0.97927	4496.57438	998.18098
SR1805_PS3_CTD84	2018-04-08T15:16:35	OMZ PS3 coastaL	depth profile	1028	CTD84	84	7	17.68324	-102.35008	99.798	26.0478	34.8174	14.0061	0.038755	-0.942	2.52	20.86	7.98	16.24	1.43	0.000	-0.00548	0.01012	n	0.02940	0.00740	y					0.97253	2229.88141	291.56998
SR1805_PS3_CTD84	2018-04-08T15:16:35	OMZ PS3 coastaL	depth profile	1028	CTD84	84	5	17.68330	-102.35000	159.859	26.3714	34.8095	12.3833	0.023830	-1.015	2.79	21.95	3.47	20.11	1.55	0.020	-0.00007	0.00110	y	0.00614	0.00166	y					0.98233	2132.89048	439.85544
SR1805_PS3_CTD84	2018-04-08T15:16:35	OMZ PS3 coastaL	depth profile	1028	CTD84	84	3	17.68326	-102.35002	249.355	26.4915	34.7644	11.5793	0.022557	-0.947		22.65	4.89	14.45	1.50	0.000	0.00041	0.00111	n	0.00662	0.00488	n					0.97651	1093.29684	88.03708
SR1805_PS3_CTD84	2018-04-08T15:16:35	OMZ PS3 coastaL	depth profile	1028	CTD84	84	1	17.68326	-102.35002	800.632	27.2095	34.5526	5.9902	0.018805	-0.666	0.00	41.16	5.26	43.62	3.27	0.400	-0.00023	0.00252	n	0.03452	0.01243	y					0.97034	2262.61880	198.60941