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Marasco, Ramona: Larval growth of the Black Soldier Fly Hermetia illucens reared in the laboratory on three different diets [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.939665 (dataset in review), In: Marasco, R: Growth bioassay of Black Soldier Fly Hermetia illucens from Italy on three different diets [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.939683 (dataset in review)

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Abstract:
Hermetia illucens individuals were reared at the entomological facilities of the University of Milan (45°28'33.9N, 9°13'39.9E). The Black Soldier Flies (BSFs) used in this work originated from a laboratory stock culture of H. illucens that was retrieved from a compost container in Lombardy, Northern Italy (45°19ʹ54ʺN, 9°05ʹ58ʺE); refer to Jucker et al., 2017, doi:10.1093/ee/nvx154. Insects were reared on three different diets: a full nutrient (FN) diet, composed by 50% wheat germ, 30% alfalfa, and 20% corn flour, to which an equal volume of water was added; a nutrient restriction fruit (NRF) diet formed by mixing fresh apples, pears and oranges (33.3% each); and a nutrient restriction vegetable (NRV) diet composed by fresh green beans, cabbage and lettuce (33% each). In the laboratory, the BSF eggs were transferred into plastic containers (10.5 × 5 cm), with metal mesh to allow air exchange. To each of the containers one of the diets (FN, NRF or NRV) was added for a total of 3 containers per diet. These containers were kept in a climate chamber under controlled conditions (temperature: 25°C ± 0.5°C; relative humidity: 60% ± 5%; photoperiod: 12:12 light:dark). The larvae were provided ad libitum access to the three diets for up to 83 days (UNTIL PUPAE EMERGED?). Wet mass was determined of groups of 10 individuals usually every 2-3 days.
Keyword(s):
Diets; Dysbiosis; Hermetia; Laboratory experiment; microbiome; Nutrient restriction
Supplement to:
Marasco, Ramona; Fusi, Marco; Callegari, Matteo; Jucker, Costanza; Mapelli, Francesca; Borin, Sara; Savoldelli, Sara; Daffonchio, Daniele; Crotti, Elena (2022): Destabilization of the Bacterial Interactome Identifies Nutrient Restriction-Induced Dysbiosis in Insect Guts. Microbiology Spectrum, 10(1), e01580-21, https://doi.org/10.1128/spectrum.01580-21
Further details:
Jucker, Constanza; Erba, Daniela; Leonardi, Maria Giovanna; Lupi, Daniela; Savoldelli, Sara (2017): Assessment of vegetable and fruit substrates as potential rearing media for Hermetia illucens (Diptera: Stratiomyidae) larvae. Environmental Entomology, 46(6), 1415-1423, https://doi.org/10.1093/ee/nvx154
Coverage:
Latitude: 45.331667 * Longitude: 9.099444
Date/Time Start: 2019-11-01T00:00:00 * Date/Time End: 2020-01-23T00:00:00
Event(s):
Lombardy_2015 * Latitude: 45.331667 * Longitude: 9.099444 * Date/Time: 2015-03-01T00:00:00 * Location: Lombardy, Italy * Comment: Collection site of Hermetia illucens for stock culture
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Type of studyStudy typeMarasco, Ramona
2DATE/TIMEDate/TimeMarasco, RamonaGeocode – experiment
3Experiment durationExp durationdaysMarasco, Ramona
4SpeciesSpeciesMarasco, Ramona
5Uniform resource locator/link to referenceURL refMarasco, RamonaITIS TSN
6Life stageLife stageMarasco, Ramona
7Treatment: foodT:foodMarasco, Ramona
8Number of individualsInd No#Marasco, Ramona
9Hermetia illucens, wet massH. illucens wmgMarasco, RamonaAnalytical balance, Sartorius CP64 (Sartorius AG, Göttingen, Germany)
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
3312 data points

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