/* DATA DESCRIPTION: Citation: Dede, Bledina; Meyerdierks, Anke (2021): Niche differentiation of sulfur-oxidizing bacteria (SUP05) in submarine hydrothermal plumes [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.939214 Abstract: Chemistry and microbial cell numbers of two bathy- (Brothers volcano; BrV-cone and northwest caldera; NWC) and a mesopelagic (Macauley volcano; McV) plumes on the Kermadec intra-oceanic arc in the South Pacific Ocean. The microbial cell numbers were determined using Catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH). Chemical measurements of DFe, DOC, TDN, PO4, NOx and SPE-DOC in the plumes are given. Keyword(s): Candidatus Thioglobus; intraoceanic arc; metabolic flexibility; Metagenome; metatranscriptome; microbial ecology; volcanic arc Related to: Dede, Bledina; Hansen, Christian T; Neuholz, René; Schnetger, Bernhard; Kleint, Charlotte; Walker, Sharon L; Bach, Wolfgang; Amann, Rudolf; Meyerdierks, Anke (2022): Niche differentiation of sulfur-oxidizing bacteria (SUP05) in submarine hydrothermal plumes. The ISME Journal, https://doi.org/10.1038/s41396-022-01195-x Further details: Specifications for CARD-FISH probes. https://download.pangaea.de/reference/111180/attachments/dede_2021_ISME_PangaeaSubmission_probe.txt Funding: Bundesministerium für Bildung und Forschung, Bonn (BMBF) (URI: https://www.bmbf.de), grant/award no. 03G0253: Geosphere-Biosphere Interactions Coverage: MEDIAN LATITUDE: -32.279008 * MEDIAN LONGITUDE: -179.837148 * SOUTH-BOUND LATITUDE: -35.258350 * WEST-BOUND LONGITUDE: 178.777120 * NORTH-BOUND LATITUDE: -28.259600 * EAST-BOUND LONGITUDE: -178.433730 DATE/TIME START: 2016-12-24T22:01:00 * DATE/TIME END: 2017-01-16T10:13:00 MINIMUM DEPTH, water: 195 m * MAXIMUM DEPTH, water: 1750 m Event(s): SO253_1-1 * LATITUDE: -28.259600 * LONGITUDE: -179.075180 * DATE/TIME: 2016-12-24T22:01:00 * ELEVATION: -2039.0 m * LOCATION: South Pacific Ocean * CAMPAIGN: SO253 (HYDROTHERMADEC) (URI: https://doi.org/10.2312/cr_so253) * BASIS: Sonne_2 (URI: https://uol.de/icbm/forschungsplattformen-schiffe/sonne) * METHOD/DEVICE: CTD/Rosette (CTD-RO) SO253_4-1 * LATITUDE: -30.209320 * LONGITUDE: -178.433730 * DATE/TIME: 2016-12-26T05:28:00 * ELEVATION: -575.0 m * LOCATION: South Pacific Ocean * CAMPAIGN: SO253 (HYDROTHERMADEC) (URI: https://doi.org/10.2312/cr_so253) * BASIS: Sonne_2 (URI: https://uol.de/icbm/forschungsplattformen-schiffe/sonne) * METHOD/DEVICE: CTD/Rosette (CTD-RO) SO253_10-1 * LATITUDE: -30.214650 * LONGITUDE: -178.443570 * DATE/TIME: 2016-12-27T10:02:00 * ELEVATION: -461.0 m * LOCATION: South Pacific Ocean * CAMPAIGN: SO253 (HYDROTHERMADEC) (URI: https://doi.org/10.2312/cr_so253) * BASIS: Sonne_2 (URI: https://uol.de/icbm/forschungsplattformen-schiffe/sonne) * METHOD/DEVICE: CTD/Rosette (CTD-RO) SO253_49-1 * LATITUDE: -34.862700 * LONGITUDE: 179.089820 * DATE/TIME: 2017-01-08T08:08:00 * ELEVATION: -1760.0 m * LOCATION: South Pacific Ocean * CAMPAIGN: SO253 (HYDROTHERMADEC) (URI: https://doi.org/10.2312/cr_so253) * BASIS: Sonne_2 (URI: https://uol.de/icbm/forschungsplattformen-schiffe/sonne) * METHOD/DEVICE: CTD/Rosette (CTD-RO) SO253_54-1 * LATITUDE: -34.869430 * LONGITUDE: 179.062650 * DATE/TIME: 2017-01-09T11:12:00 * ELEVATION: -1805.0 m * LOCATION: South Pacific Ocean * CAMPAIGN: SO253 (HYDROTHERMADEC) (URI: https://doi.org/10.2312/cr_so253) * BASIS: Sonne_2 (URI: https://uol.de/icbm/forschungsplattformen-schiffe/sonne) * METHOD/DEVICE: CTD/Rosette (CTD-RO) SO253_79-1 * LATITUDE: -35.258350 * LONGITUDE: 178.777120 * DATE/TIME: 2017-01-16T10:13:00 * ELEVATION: -3151.0 m * LOCATION: South Pacific Ocean * CAMPAIGN: SO253 (HYDROTHERMADEC) (URI: https://doi.org/10.2312/cr_so253) * BASIS: Sonne_2 (URI: https://uol.de/icbm/forschungsplattformen-schiffe/sonne) * METHOD/DEVICE: CTD/Rosette (CTD-RO) Parameter(s): Event label (Event) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) Latitude of event (Latitude) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) Longitude of event (Longitude) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) Date/Time of event (Date/Time) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) DEPTH, water [m] (Depth water) * GEOCODE * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) Sample ID (Sample ID) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) Origin (Origin) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * COMMENT: Source Type (Type) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * COMMENT: Cast type Location (Location) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * COMMENT: Plume area Turbidity (Nephelometric turbidity unit) [NTU] (Turbidity) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * METHOD/DEVICE: CTD, SEA-BIRD SBE 9 plus Temperature, water [°C] (Temp) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * METHOD/DEVICE: CTD, SEA-BIRD SBE 9 plus pH (pH) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) Oxygen [µmol/kg] (O2) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) Iron, dissolved [nmol/l] (Fe diss) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * METHOD/DEVICE: High Resolution Sector Field Inductively Coupled Plasma Mass Spectrometry (HR-SF-ICP-MS) Carbon, organic, dissolved [µmol/l] (DOC) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * METHOD/DEVICE: High temperature catalytic combustion Nitrogen, total dissolved [µmol/l] (TDN) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * METHOD/DEVICE: High temperature catalytic combustion Phosphate [µmol/l] ([PO4]3-) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * METHOD/DEVICE: Colorimetric analysis Nitrogen oxide [µmol/l] (NOx) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * METHOD/DEVICE: Colorimetric analysis Hydrogen sulfide [µmol/l] (H2S) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * METHOD/DEVICE: Modeled Carbon, organic, dissolved, extracted [µmol/l] (DOC extr) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * METHOD/DEVICE: Solid-phase extracted (Dittmer etal, 2008) (SPE) (URI: https://doi.org/10.4319/lom.2008.6.230) * COMMENT: solid-phase extractable dissolved organic carbon Analysis (Analysis) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * COMMENT: CARD FISH Analysis (Analysis) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * COMMENT: Metagenome Analysis (Analysis) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * COMMENT: Metatranscriptome Prokaryotic cell abundance [#/cm**3] (P cell abund) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) Bacteria, targed with EUB338(I-III) oligonucleotide FISH-probe [%] (EUB338 I-III) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * METHOD/DEVICE: Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) (URI: https://doi.org/10.1128/AEM.68.6.3094-3101.2002) Archaea, targed with ARCH915 oligonucleotide FISH-probe [%] (ARCH915) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * METHOD/DEVICE: Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) (URI: https://doi.org/10.1128/AEM.68.6.3094-3101.2002) Gammaproteobacteria, targeted with Gam42a oligonucleotide FISH-probe [%] (Gam42a) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * METHOD/DEVICE: Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) (URI: https://doi.org/10.1128/AEM.68.6.3094-3101.2002) Gammaproteobacteria, targed with the SUP05_1241 oligonucleotide FISH-probe [%] (SUP05_1241 SUP05 cluster) * PI: Dede, Bledina (https://orcid.org/0000-0001-5244-5499, bdede@mpi-bremen.de) * METHOD/DEVICE: Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) (URI: https://doi.org/10.1128/AEM.68.6.3094-3101.2002) License: Creative Commons Attribution 4.0 International (CC-BY-4.0) (URI: https://creativecommons.org/licenses/by/4.0/) Status: Curation Level: Enhanced curation (URI: https://wiki.pangaea.de/wiki/Curation_levels) Size: 526 data points */ Event Latitude Longitude Date/Time Depth water [m] Sample ID Origin (Source) Type (Cast type) Location (Plume area) Turbidity [NTU] (CTD, SEA-BIRD SBE 9 plus) Temp [°C] (CTD, SEA-BIRD SBE 9 plus) pH O2 [µmol/kg] Fe diss [nmol/l] (High Resolution Sector Field ...) DOC [µmol/l] (High temperature catalytic co...) TDN [µmol/l] (High temperature catalytic co...) [PO4]3- [µmol/l] (Colorimetric analysis) NOx [µmol/l] (Colorimetric analysis) H2S [µmol/l] (Modeled) DOC extr [µmol/l] (solid-phase extractable disso...) Analysis (CARD FISH) Analysis (Metagenome) Analysis (Metatranscriptome) P cell abund [#/cm**3] EUB338 I-III [%] (Catalysed reporter deposition...) ARCH915 [%] (Catalysed reporter deposition...) Gam42a [%] (Catalysed reporter deposition...) SUP05_1241 SUP05 cluster [%] (Catalysed reporter deposition...) SO253_1-1 -28.2596 -179.0752 2016-12-24T22:01:00 402 01CTD_b19 Background Vertical Backg. 13.6 184.20 - - - SO253_79-1 -35.2584 178.7771 2017-01-16T10:13:00 1499 79CTD_b6 Background Vertical Backg. 3.8 7.80 159.90 172 39 20.00 - - - SO253_4-1 -30.2093 -178.4337 2016-12-26T05:28:00 248 04CTD_b2 Macauley Tow-yo Plume 0.0534 15.1 8.00 186.70 7.20 177 10 0.400 7.100 65.7 2.78 + - - 127018.66260 43 23 6 10 SO253_4-1 -30.2093 -178.4337 2016-12-26T05:28:00 221 04CTD_b4 Macauley Tow-yo Above 0.0021 15.6 8.00 188.30 3.80 194 10 0.400 5.900 34.3 31.00 + - - 112208.59340 45 25 2 0 SO253_4-1 -30.2093 -178.4337 2016-12-26T05:28:00 287 04CTD_b6 Macauley Tow-yo Max 0.2021 15.0 7.90 178.70 126.00 199 11 0.500 8.100 1148.8 19.80 + + + 117863.27360 29 19 4 14 SO253_4-1 -30.2093 -178.4337 2016-12-26T05:28:00 351 04CTD_b8 Macauley Tow-yo Below 0.0055 13.0 8.00 187.10 6.00 169 14 0.700 12.000 54.4 26.40 + - - 107158.99130 44 21 4 2 SO253_4-1 -30.2093 -178.4337 2016-12-26T05:28:00 250 04CTD_b10 Macauley Tow-yo No sig. 0.0024 15.0 8.00 187.10 3.80 162 10 0.500 7.000 34.3 26.70 + - - 118306.63990 31 22 6 0 SO253_10-1 -30.2146 -178.4436 2016-12-27T10:02:00 253 10CTD_b2 Macauley Tow-yo Plume 0.0345 15.1 8.00 187.70 9.50 0.500 7.000 87.1 + + - 93619.85388 52 21 4 23 SO253_10-1 -30.2146 -178.4436 2016-12-27T10:02:00 274 10CTD_b4 Macauley Tow-yo Plume 0.2777 14.8 7.80 183.00 22.20 291 11 0.600 7.400 202.3 27.00 + + + 147050.63710 37 21 3 23 SO253_10-1 -30.2146 -178.4436 2016-12-27T10:02:00 195 10CTD_b10 Macauley Tow-yo Above 0.0227 16.2 7.90 188.10 6.10 299 9 0.300 5.500 55.2 24.00 + + - 142098.58210 43 25 1 0 SO253_10-1 -30.2146 -178.4436 2016-12-27T10:02:00 264 10CTD_b12 Macauley Tow-yo Plume 0.0654 14.9 7.90 186.80 5.50 0.500 6.800 50.0 + + - 115743.58550 43 22 3 5 SO253_10-1 -30.2146 -178.4436 2016-12-27T10:02:00 274 10CTD_b17 Macauley Tow-yo Weak 0.0260 14.9 7.90 186.80 12.80 257 17 0.900 8.500 23.00 + - - 119147.54330 36 21 3 2 SO253_10-1 -30.2146 -178.4436 2016-12-27T10:02:00 276 10CTD_b19 Macauley Tow-yo No sig 0.0186 14.8 7.90 186.40 12.50 242 11 0.500 8.100 28.00 + - - 119524.12580 44 24 2 1 SO253_49-1 -34.8627 179.0898 2017-01-08T08:08:00 1335 49CTD_b2 Brothers NW Caldera Tow-yo Plume 0.0208 4.2 7.80 168.30 6.00 45 33 2.200 31.900 2.2 22.00 + - - 31393.19767 65 10 5 8 SO253_49-1 -34.8627 179.0898 2017-01-08T08:08:00 1496 49CTD_b4 Brothers NW Caldera Tow-yo Plume 0.0107 3.3 7.80 155.04 9.60 43 35 2.200 31.400 3.5 19.00 - - - SO253_49-1 -34.8627 179.0898 2017-01-08T08:08:00 1229 49CTD_b6 Brothers upper cone Tow-yo Above 0.0118 4.9 7.80 178.30 20.60 47 31 2.100 28.100 7.4 20.00 - - - SO253_49-1 -34.8627 179.0898 2017-01-08T08:08:00 1538 49CTD_b8 Brothers NW Caldera Tow-yo Plume 0.1103 3.4 7.80 156.00 15.30 44 34 2.300 31.300 5.5 20.00 - - - SO253_49-1 -34.8627 179.0898 2017-01-08T08:08:00 1308 49CTD_b10 Brothers lower cone Tow-yo Plume 0.0042 4.5 7.80 171.10 11.70 44 33 2.200 30.800 4.2 20.00 - + - SO253_49-1 -34.8627 179.0898 2017-01-08T08:08:00 1280 49CTD_b12 Brothers lower cone Tow-yo Plume 0.0136 4.6 7.80 171.60 25.10 44 32 2.100 29.700 9.0 20.00 - - - SO253_49-1 -34.8627 179.0898 2017-01-08T08:08:00 1304 49CTD_b16 Brothers lower cone Tow-yo Plume 0.0226 4.3 7.60 167.60 18.80 48 33 2.100 29.600 6.8 23.00 - + - SO253_49-1 -34.8627 179.0898 2017-01-08T08:08:00 1556 49CTD_b18 Brothers NW Caldera Tow-yo Weak 0.0049 3.2 8.02 153.40 1.73 59 10 0.500 5.500 0.6 24.00 - - - SO253_54-1 -34.8694 179.0626 2017-01-09T11:12:00 1750 54CTD_b4 Brothers NW Caldera Vertical Plume 0.0466 3.3 7.70 153.10 15.50 42 35 2.200 35.000 5.6 20.00 +- - - 31058.54937 75 16 0 56 SO253_54-1 -34.8694 179.0626 2017-01-09T11:12:00 1560 54CTD_b8 Brothers NW Caldera Vertical Plume 0.1222 3.4 7.80 154.70 34.60 42 34 2.200 35.000 12.5 19.00 + + + 43214.50280 80 8 1 67 SO253_54-1 -34.8694 179.0626 2017-01-09T11:12:00 1440 54CTD_b12 Brothers NW Caldera Vertical Plume 0.0475 3.5 7.80 157.80 12.70 44 35 2.200 34.700 4.6 19.00 + + + 38250.22256 59 17 1 26 SO253_54-1 -34.8694 179.0626 2017-01-09T11:12:00 1371 54CTD_b15 Brothers NW Caldera Vertical Between 0.0025 3.8 7.80 152.50 6.80 42 34 2.200 34.300 2.4 20.00 + - - 26542.56794 52 19 2 9 SO253_54-1 -34.8694 179.0626 2017-01-09T11:12:00 1229 54CTD_b18 Brothers upper cone Vertical Above 0.0124 4.6 7.80 147.70 22.20 46 33 2.080 32.300 8.0 21.00 - - -