Citation:

PANGAEA.938692

Name: High variability in SSU rDNA gene copy number among planktonic foraminifera revealed by single-cell qPCR
Published: 2021-11-29
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Foraminifera; gene copy number; quantitative PCR

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Abstract:

Metabarcoding has become the workhorse of community ecology. Sequencing a taxonomically informative DNA fragment from environmental samples gives fast access to community composition across taxonomic groups, but it relies on the assumption that the number of sequences for each taxon correlates with its abundance in the sampled community. However, gene copy number varies among and within taxa, and the extent of this variability must therefore be considered when interpreting community composition data derived from environmental sequencing. Here we measured with single-cell qPCR the SSU rDNA gene copy number of 139 specimens of five species of planktonic foraminifera. We found that the average gene copy number varied between of ~4 000 to ~50 000 gene copies between species, and individuals of the same species can carry between ~300 to more than 350 000 gene copies. This variability cannot be explained by differences in cell size and considering all plausible sources of bias, we conclude that this variability likely reflects dynamic genomic processes acting during the life cycle. We used the observed variability to model its impact on metabarcoding and found that the application of a correcting factor at species level may correct the derived relative abundances, provided sufficiently large populations have been sampled.

Keyword(s):

Foraminifera; gene copy number; quantitative PCR

Related to:

Milivojević, Tamara; Rahman, Shirin Nurshan; Raposo, Débora; Siccha, Michael; Kucera, Michal; Morard, Raphael (2021): High variability in SSU rDNA gene copy number among planktonic foraminifera revealed by single-cell qPCR. ISME Communications, 1(1), https://doi.org/10.1038/s43705-021-00067-3

Project(s):

Center for Marine Environmental Sciences (MARUM)

Coverage:

Median Latitude: -15.636116 * Median Longitude: -22.066239 * South-bound Latitude: -38.772500 * West-bound Longitude: -67.000367 * North-bound Latitude: 71.999950 * East-bound Longitude: 17.584500

Date/Time Start: 2015-01-31T12:05:00 * Date/Time End: 2017-08-19T17:35:00

Minimum DEPTH, water: 10 m * Maximum DEPTH, water: 150 m

Event(s):

M113/2_44-2 (GeoB19612-2) * Latitude: 28.583170 * Longitude: -49.364000 * Date/Time: 2015-01-31T12:05:00 * Elevation: -4374.0 m * Campaign: (SpaDE) * Basis: * Method/Device: Multiple opening/closing net (MSN)

M113/2_44-3 (GeoB19612-3) * Latitude: 28.583330 * Longitude: -49.364000 * Date/Time: 2015-01-31T12:37:00 * Elevation: -4370.0 m * Campaign: (SpaDE) * Basis: * Method/Device: Multiple opening/closing net (MSN)

M124_0344_K349 * Latitude: -29.180000 * Longitude: -20.053000 * Campaign: * Basis: * Method/Device: Multiple opening/closing net (MSN)

Comment:

Supplementary Material 1. Geographic origin, taxonomic identification and SSU rDNA gene copy number quantification obtain with qPCR for the 139 specimens. Sequences are provided for the fragment 45E-47F.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Comment
1	Event label	Event		Morard, Raphael
2	Latitude of event	Latitude		Morard, Raphael
3	Longitude of event	Longitude		Morard, Raphael
4	Elevation of event	Elevation	m	Morard, Raphael
5	Voucher Specimen Code	Voucher Spec Code		Morard, Raphael
6	Cruise/expedition	Expedition		Morard, Raphael
7	Station label	Station		Morard, Raphael
8	DEPTH, water	Depth water	m	Morard, Raphael	Geocode
9	Depth, top/min	Depth top	m	Morard, Raphael
10	Depth, bottom/max	Depth bot	m	Morard, Raphael
11	Species	Species		Morard, Raphael
12	Primers	Primers		Morard, Raphael
13	Region, genetic	Region		Morard, Raphael	SSU region
14	Genetic sequence	Gensequence		Morard, Raphael
15	Gene copies per specimen	GCN per spec	#	Morard, Raphael
16	Gene copies per specimen, normalized, standard deviation	GCN per spec norm	±	Morard, Raphael
17	Gene copies per specimen, minimum	GCN per spec min	#	Morard, Raphael
18	Gene copies per specimen, maximum	GCN per spec max	#	Morard, Raphael
19	Mid sphere test volume	Mid sphere vol	mm³	Morard, Raphael
20	Gene copies per area	GCN per area	mm³	Morard, Raphael

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2505 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 Latitude	3 Longitude	4 Elevation [m]	5 Voucher Spec Code	6 Expedition	7 Station	8 Depth water [m]	9 Depth top [m]	10 Depth bot [m]	11 Species	12 Primers	13 Region	14 Gensequence	15 GCN per spec [#]	16 GCN per spec norm [±]	17 GCN per spec min [#]	18 GCN per spec max [#]	19 Mid sphere vol [mm³]	20 GCN per area [mm³]
M113/2_44-2	28.5832	-49.3640	-4374	T299	M113-2	GeoB19612-02	150	100	200	G. siphonifera	S18F-S19F	45E-47F	GCTACATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTGGATTGGTGGTGAATTGTTGGCCCCGTCTAATACTGTCCTGTCTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTACTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGTAACCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGG	1818.4791	308.6319	1509.8472	2127.1109	0.005902050	308109.7178
M113/2_44-3	28.5833	-49.3640	-4370	T316	M113-2	GeoB19612-03	30	20	40	G. glutinata	S18F-S19F	45E-47F	ATGATTGTTGCAGTGAGCATCTCAATTCTTTACCTTAACACCGCACACGTGAGTATTAACGTACTTTACGCAGCGGTAAAGCCTGCTTCGAGAGCAAGTGGGTAATCAATTAGAAGTAACGATTTCCCAAGTTAGCACACCTATATACGGCGTTTGTGCCCGGGTTTCACTTGTTGGAGCTTTTGTGCGCGCAGATGTTTCT	2711.9230	145.1898	2566.7331	2857.1128	0.000711539	3811346.9170
M113/2_44-3	28.5833	-49.3640	-4370	T317	M113-2	GeoB19612-03	30	20	40	G. glutinata	S18F-S19F	45E-47F	ATGATTGTTGCAGTGAAGCATCTCAATTCTTTACCTTAACACCGCACACGTGAGTATTAACGTACTTTACGCAGCGGTAAAGCCTGCTTCGAGAGCAAGTGGGTAATCAATTAGAAGTAACGATTTCCCAAGTTAGCACACCTATATACGGCGTTTGTGCCCGGGTTTCACTTGTTGGAGCTTTTGTGCGCGCAGATGTTTCTTCC	258.5164	82.6333	175.8831	341.1497	0.000501357	515633.1386
M124_0344_K349	-29.1800	-20.0530		T220	M124	K349	30	20	40	T. sacculifer	S14p-S15r2	37F	NA	8118.8652	113.2308	8005.6344	8232.0960	0.004464641	1818481.2610
M124_0344_K349	-29.1800	-20.0530		T222	M124	K349	30	20	40	T. sacculifer	S14p-S15r2	37F	NA	16688.5132	973.7967	15714.7165	17662.3099	0.002133362	7822633.7340
M124_0344_K349	-29.1800	-20.0530		T223	M124	K349	30	20	40	T. sacculifer	S14p-S15r2	37F	NA	16969.4008	677.2574	16292.1434	17646.6582	0.004191168	4048847.4830
M124_0344_K349	-29.1800	-20.0530		T220	M124	K349	30	20	40	T. sacculifer	S18F-S19F	45E-47F	GCTACAATGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGGGA	2486.8720	495.7129	1991.1592	2982.5849	0.004464641	557015.0610
M124_0344_K349	-29.1800	-20.0530		T222	M124	K349	30	20	40	T. sacculifer	S18F-S19F	45E-47F	GAATGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGGGA	3045.2637	872.8078	2172.4559	3918.0715	0.002133362	1427447.8840
M124_0344_K349	-29.1800	-20.0530		T223	M124	K349	30	20	40	T. sacculifer	S18F-S19F	45E-47F	GCTACAATGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGGG	1111.4719	157.3319	954.1400	1268.8039	0.004191168	265193.8285
M133_1678-1	-34.3828	17.5835	-2864	T400	M133	ME1330/1678	20	0	40	T. sacculifer	S14p-S15r2	37F	NA	30227.9622	2968.4421	27259.5201	33196.4043	0.003318427	9109123.7140
M133_1678-1	-34.3828	17.5835	-2864	T491	M133	ME1330/1678	20	0	40	T. sacculifer	S14p-S15r2	37F	NA	163164.6769	17938.7981	145225.8788	181103.4750	0.008798672	18544239.6800
M133_1678-1	-34.3828	17.5835	-2864	T493	M133	ME1330/1678	20	0	40	T. sacculifer	S14p-S15r2	37F	NA	1805.7126	81.2932	1724.4193	1887.0058	0.003506965	514893.2713
M133_1678-1	-34.3828	17.5835	-2864	T494	M133	ME1330/1678	20	0	40	T. sacculifer	S14p-S15r2	37F	NA	66197.6603	6488.3995	59709.2608	72686.0598	0.005224154	12671460.2600
M133_1678-1	-34.3828	17.5835	-2864	T688	M133	ME1330/1678	20	0	40	T. sacculifer	S14p-S15r2	37F	NA	23376.5874	4036.0604	19340.5271	27412.6478	0.004977580	4696375.7410
M133_1678-1	-34.3828	17.5835	-2864	T400	M133	ME1330/1678	20	0	40	T. sacculifer	S18F-S19F	45E-47F	ATGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGGG	5839.4465	1546.6968	4292.7497	7386.1433	0.003318427	1759703.1610
M133_1678-1	-34.3828	17.5835	-2864	T491	M133	ME1330/1678	20	0	40	T. sacculifer	S18F-S19F	45E-47F	TGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGGGA	42897.2076	4504.0591	38393.1485	47401.2668	0.008798672	4875418.5950
M133_1678-1	-34.3828	17.5835	-2864	T494	M133	ME1330/1678	20	0	40	T. sacculifer	S18F-S19F	45E-47F	CTACAATGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGGG	29092.2638	1041.0765	28051.1873	30133.3403	0.005224154	5568799.0010
M133_1678-1	-34.3828	17.5835	-2864	T496	M133	ME1330/1678	20	0	40	G. siphonifera	S18F-S19F	45E-47F	TGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGGA	19676.6032	3491.9634	16184.6398	23168.5666	0.014847765	1325223.2110
M133_1678-1	-34.3828	17.5835	-2864	T500	M133	ME1330/1678	20	0	40	G. siphonifera	S18F-S19F	45E-47F	TGCTACATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGG	5936.6742	876.4518	5060.2225	6813.1260	0.000974443	6092378.3880
M133_1678-1	-34.3828	17.5835	-2864	T671	M133	ME1330/1678	20	0	40	G. glutinata	S18F-S19F	45E-47F	CTACATGATTGTTGCAGTGAGCATCTCAATTTTTTACCTTAACACCGCACACGTGAGTAGCATACTTGTATGTTTACTTTACGCAGCGGTAAAGCCTGCTTCGAGAGCAAGTGGGTAATCAATTAGAAGTAACGATTTCCCAAATTAGCACACTTATATGCGGCGTTTGCGCCCGGGTGCCACTTGTTGGTACTTCTGTGCGTGCAGATGTTTTTTTT	11007.7858	1458.1524	9549.6334	12465.9381	0.001935307	5687874.7330
M133_1678-1	-34.3828	17.5835	-2864	T672	M133	ME1330/1678	20	0	40	G. glutinata	S18F-S19F	45E-47F	TACATGATTGTTGCAGTGAGCATCTCAATTTTTTACCTAACACCGCACACGTGAGTGCATACTTGTATGTTACTTTACGCAGCGGTAAAGCCTGCTTCGAGAGCAAGTGGGTAATCAATTAGAAGTAACGATTTCCCAAATTAGCACACTTATATACGGCGTTTGTGCCCGGGTTTCACTTGTTGGAGCTTTTGTGCGCGCAGATGTTTTTTCCGT	5779.9089	41.2597	5738.6493	5821.1686	0.002540924	2274727.5980
M133_1678-1	-34.3828	17.5835	-2864	T675	M133	ME1330/1678	20	0	40	G. glutinata	S18F-S19F	45E-47F	TGATTGTTGCAGTGAGCATCTCAATTTTTTACCTAACACCGCACACGTGAGTGCATACTTGTATGTTACTTTACGCAGCGGTAAAGCCTGCTTCGAGAGCAAGTGGGTAATCAATTAGAAGTAACGATTTCCCAAATTAGCACACTTATATACGGCGTTTGTGCCCGGGTTTCACTTGTTGGAACTTTTGTGCGCGCAGATGTTTTTT	1557.8353	181.7960	1376.0392	1739.6313	0.002385028	653172.7454
M133_1678-1	-34.3828	17.5835	-2864	T679	M133	ME1330/1678	20	0	40	G. glutinata	S18F-S19F	45E-47F	CAATGATTGTTGCAGTGAGCATCTCAATTTTTTACCTAACACCGCACACGTGAGTGCATACTTGTATGTTACTTTACGCAGCGGTAAAGCCTGCTTCGAGAGCAAGTGGGTAATCAATTAGAAGTAACGATTTCCCAAATTAGCACACTTATATGCGGCGTTTGCGCCCGGGTATCACTTGTTGGTACTTCTGTGCGTGCAGATGTTTTTTTCCGCATGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	1365.6978	458.3970	907.3008	1824.0949	0.000851808	1603292.1630
M133_1678-1	-34.3828	17.5835	-2864	T688	M133	ME1330/1678	20	0	40	T. sacculifer	S18F-S19F	45E-47F	CTACAATGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGG	7658.0668	609.0632	7049.0036	8267.1300	0.004977580	1538511.9540
M133_1685-1	-36.1522	15.6662	-4682	T066	M133	ME1330/1685	70	60	80	T. sacculifer	S14p-S15r2	37F	NA	128615.9342	17877.3396	110738.5946	146493.2739	0.012170680	10567687.0400
M133_1685-1	-36.1522	15.6662	-4682	T634	M133	ME1330/1685	30	20	40	T. sacculifer	S14p-S15r2	37F	NA	5843.0285	777.3742	5065.6544	6620.4027	0.017928669	325904.2033
M133_1685-1	-36.1522	15.6662	-4682	T640	M133	ME1330/1685	30	20	40	T. sacculifer	S14p-S15r2	37F	NA	7788.8733	1709.8941	6078.9792	9498.7674	0.004637904	1679395.1700
M133_1685-1	-36.1522	15.6662	-4682	T802	M133	ME1330/1685	10	0	20	N. dutertrei	S18F-S19F	45E-47F	CTACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	291.8739	80.0142	211.8597	371.8881	0.010646847	27414.1150
M133_1685-1	-36.1522	15.6662	-4682	T803	M133	ME1330/1685	10	0	20	N. dutertrei	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAA	2317.6338	705.0786	1612.5551	3022.7124	0.005098395	454581.0723
M133_1685-1	-36.1522	15.6662	-4682	T807	M133	ME1330/1685	30	20	40	N. dutertrei	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCCCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	61607.4931	2089.4932	59517.9999	63696.9863	0.011483052	5365080.2340
M133_1685-1	-36.1522	15.6662	-4682	T808	M133	ME1330/1685	30	20	40	N. dutertrei	S18F-S19F	45E-47F	GTGCTACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTGGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCCAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	1442.5171	280.5331	1161.9840	1723.0501	0.009123427	158111.3137
M133_1685-1	-36.1522	15.6662	-4682	T811	M133	ME1330/1685	30	20	40	N. dutertrei	S18F-S19F	45E-47F	TACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	13205.1203	1293.2768	11911.8435	14498.3972	0.003191151	4138042.6420
M133_1685-1	-36.1522	15.6662	-4682	T815	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	GATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGGA	127930.1636	8426.0395	119504.1240	136356.2031	0.006619671	19325758.6400
M133_1685-1	-36.1522	15.6662	-4682	T816	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	CTACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	17419.9437	1436.4943	15983.4495	18856.4380	0.009119213	1910246.3740
M133_1685-1	-36.1522	15.6662	-4682	T817	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	TACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	6171.2446	109.6356	6061.6089	6280.8802	0.007946163	776632.0203
M133_1685-1	-36.1522	15.6662	-4682	T829	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTTCGTACGGGA	31304.7038	2797.7725	28506.9313	34102.4763	0.009745846	3212107.4490
M133_1685-1	-36.1522	15.6662	-4682	T831	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	TAAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	1403.4481	337.5263	1065.9217	1740.9744	0.008800469	159474.2337
M133_1685-1	-36.1522	15.6662	-4682	T832	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	ATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCCCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	10538.9682	312.1275	10226.8407	10851.0956	0.005872938	1794496.7430
M133_1685-1	-36.1522	15.6662	-4682	T833	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	ACATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGGA	17955.7190	2575.4588	15380.2602	20531.1778	0.009255212	1940065.5820
M133_1685-1	-36.1522	15.6662	-4682	T834	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	GCTACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGG	3889.6852	737.7968	3151.8884	4627.4820	0.004840325	803599.9405
M133_1685-1	-36.1522	15.6662	-4682	T837	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	ATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGG	16010.8785	2029.8088	13981.0698	18040.6872	0.008068290	1984420.1840
M133_1685-1	-36.1522	15.6662	-4682	T838	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATA	23897.4472	3130.4131	20767.0341	27027.8603	0.004148837	5760035.8820
M133_1685-1	-36.1522	15.6662	-4682	T839	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTTCGTACGGGA	90260.0382	30386.3749	59873.6633	120646.4132	0.008176859	11038472.9800
M133_1685-1	-36.1522	15.6662	-4682	T841	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	GCTAAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGC	626.8110	173.8030	453.0080	800.6140	0.002548992	245905.4301
M133_1685-1	-36.1522	15.6662	-4682	T845	M133	ME1330/1685	10	0	20	G. siphonifera	S18F-S19F	45E-47F	GCTACATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGG	3399.2289	433.9105	2965.3184	3833.1393	0.001000064	3399012.2440
M133_1685-1	-36.1522	15.6662	-4682	T851	M133	ME1330/1685	30	20	40	G. siphonifera	S18F-S19F	45E-47F	GATTAGCGCAGTGCGCATACTTTCAATATGATACATTGGATTGGAGTGGTCTGGTGAGCTCCGTCTAATACTGTCCTGCCTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCA	3671.3549	127.4426	3543.9122	3798.7975	0.001756573	2090066.7140
M133_1685-1	-36.1522	15.6662	-4682	T853	M133	ME1330/1685	50	40	60	G. siphonifera	S18F-S19F	45E-47F	TGCTACATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGG	5738.6323	198.4001	5540.2322	5937.0324	0.005284187	1086001.0410
M133_1685-1	-36.1522	15.6662	-4682	T855	M133	ME1330/1685	50	40	60	G. siphonifera	S18F-S19F	45E-47F	TGCTACATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGGA	2334.5714	543.0454	1791.5260	2877.6168	0.002463925	947500.9077
M133_1685-1	-36.1522	15.6662	-4682	T857	M133	ME1330/1685	50	40	60	G. siphonifera	S18F-S19F	45E-47F	GTGCTACATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGG	4116.4982	964.6648	3151.8334	5081.1629	0.003066806	1342275.3680
M133_1685-1	-36.1522	15.6662	-4682	T859	M133	ME1330/1685	50	40	60	G. siphonifera	S18F-S19F	45E-47F	TGCTACATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGG	815.2349	88.4351	726.7999	903.6700	0.003355113	242982.8482
M133_1685-1	-36.1522	15.6662	-4682	T860	M133	ME1330/1685	50	40	60	G. siphonifera	S18F-S19F	45E-47F	GCTACATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTGGATTGGTGGTGAATTGTTGGCCCCGTCTAATACTGTCCTGTCTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTACTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGTAACCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGG	1435.6287	429.7236	1005.9051	1865.3523	0.007744620	185371.1001
M133_1685-1	-36.1522	15.6662	-4682	T861	M133	ME1330/1685	50	40	60	G. siphonifera	S18F-S19F	45E-47F	ATGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGGA	4286.5522	1006.5354	3280.0168	5293.0876	0.002950164	1452987.8370
M133_1685-1	-36.1522	15.6662	-4682	T862	M133	ME1330/1685	50	40	60	G. siphonifera	S18F-S19F	45E-47F	ATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGGA	3454.9409	259.5610	3195.3799	3714.5019	0.004013601	860808.3212
M133_1685-1	-36.1522	15.6662	-4682	T864	M133	ME1330/1685	50	40	60	G. siphonifera	S18F-S19F	45E-47F	TTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGG	12225.2731	1235.2924	10989.9807	13460.5655	0.001587435	7701274.5550
M133_1685-1	-36.1522	15.6662	-4682	T865	M133	ME1330/1685	50	40	60	G. siphonifera	S18F-S19F	45E-47F	TGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGGA	6328.1422	543.6760	5784.4661	6871.8182	0.000549462	11516982.1900
M133_1685-1	-36.1522	15.6662	-4682	T866	M133	ME1330/1685	50	40	60	G. siphonifera	S18F-S19F	45E-47F	TTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATAGCTTTTGCTTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGTAATATGACTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGGA	4137.1295	299.7640	3837.3655	4436.8934	0.002379245	1738841.0970
M133_1685-1	-36.1522	15.6662	-4682	T870	M133	ME1330/1685	50	40	60	G. siphonifera	S18F-S19F	45E-47F	GCTACATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGGA	1204.0946	126.6658	1077.4288	1330.7604	0.001030799	1168117.1590
M133_1685-1	-36.1522	15.6662	-4682	T871	M133	ME1330/1685	50	40	60	G. siphonifera	S18F-S19F	45E-47F	TGCTACATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATAGCTTTTGCTTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGTAATATGACTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGGA	1904.8829	318.6178	1586.2651	2223.5007	0.001950894	976415.3782
M133_1685-1	-36.1522	15.6662	-4682	T873	M133	ME1330/1685	50	40	60	G. siphonifera	S18F-S19F	45E-47F	GACTACATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGG	1122.6158	111.5018	1011.1139	1234.1176	0.006092268	184268.9401
M133_1685-1	-36.1522	15.6662	-4682	T875	M133	ME1330/1685	70	60	80	G. siphonifera	S18F-S19F	45E-47F	ATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTGGATTGGTGGTGAATTGTTGGCCCCGTCTAATACTGTCCTGTCTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTACTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGTAACCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGGA	1685.8368	84.0962	1601.7406	1769.9330	0.006648535	253565.1506
M133_1685-1	-36.1522	15.6662	-4682	T877	M133	ME1330/1685	70	60	80	G. siphonifera	S18F-S19F	45E-47F	GATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGGA	4385.6077	771.0948	3614.5129	5156.7024	0.005275494	831316.9913
M133_1685-1	-36.1522	15.6662	-4682	T880	M133	ME1330/1685	70	60	80	G. siphonifera	S18F-S19F	45E-47F	TGCGCATACTTTCAATATGATACATTGGATTGGTGGTGAATTGTTGGCCCCGTCTAATACTGTCCTGTCTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTACTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGTAACCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGGA	457.5040	172.7697	284.7343	630.2738	0.008406539	54422.4031
M133_1685-1	-36.1522	15.6662	-4682	T881	M133	ME1330/1685	70	60	80	G. siphonifera	S18F-S19F	45E-47F	CTACATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATAGCTTTTGCTTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGTAATATGACTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGGA	7803.8900	311.7724	7492.1176	8115.6624	0.004450046	1753665.1880
M133_1685-1	-36.1522	15.6662	-4682	T884	M133	ME1330/1685	70	60	80	G. siphonifera	S18F-S19F	45E-47F	CTAATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGGA	1922.9843	479.6599	1443.3244	2402.6442	0.004626881	415611.3169
M133_1685-1	-36.1522	15.6662	-4682	T034	M133	ME1330/1685	50	40	60	T. sacculifer	S18F-S19F	45E-47F	TGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGGG	628.7514	368.9430	259.8084	997.6945	0.013111497	47954.2078
M133_1685-1	-36.1522	15.6662	-4682	T040	M133	ME1330/1685	50	40	60	T. sacculifer	S18F-S19F	45E-47F	CAATGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGGG	15307.3130	5112.6796	10194.6334	20419.9926	0.017765860	861613.9502
M133_1685-1	-36.1522	15.6662	-4682	T041	M133	ME1330/1685	50	40	60	T. sacculifer	S18F-S19F	45E-47F	TACAATGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGGG	8971.2865	885.3561	8085.9304	9856.6426	0.007622223	1176990.8970
M133_1685-1	-36.1522	15.6662	-4682	T042	M133	ME1330/1685	50	40	60	T. sacculifer	S18F-S19F	45E-47F	TGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGGGA	37983.5551	12981.8494	25001.7057	50965.4045	0.028083217	1352535.7500
M133_1685-1	-36.1522	15.6662	-4682	T044	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	GATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	44331.4346	1396.3223	42935.1124	45727.7569	0.032127399	1379863.7960
M133_1685-1	-36.1522	15.6662	-4682	T046	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	ATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	121.2203	4.1184	117.1019	125.3388	0.031618707	3833.8165
M133_1685-1	-36.1522	15.6662	-4682	T047	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	CTACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCCCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	6530.1580	418.6570	6111.5011	6948.8150	0.029973878	217861.6318
M133_1685-1	-36.1522	15.6662	-4682	T048	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	CTACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	3968.4392	921.3931	3047.0461	4889.8323	0.024431305	162432.5513
M133_1685-1	-36.1522	15.6662	-4682	T049	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	GCTACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGGA	548.6263	132.3792	416.2471	681.0054	0.022620977	24252.9868
M133_1685-1	-36.1522	15.6662	-4682	T050	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	CTACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	3839.8797	1052.9416	2786.9381	4892.8213	0.018526502	207264.1453
M133_1685-1	-36.1522	15.6662	-4682	T062	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	GATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCC	7814.9011	3830.1084	3984.7927	11645.0095	0.025122504	311071.7384
M133_1685-1	-36.1522	15.6662	-4682	T064	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	ACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	4465.4282	198.1702	4267.2580	4663.5983	0.016830354	265319.9126
M133_1685-1	-36.1522	15.6662	-4682	T065	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	2026.6190	276.4075	1750.2115	2303.0265	0.010254114	197639.6068
M133_1685-1	-36.1522	15.6662	-4682	T066	M133	ME1330/1685	70	60	80	T. sacculifer	S18F-S19F	45E-47F	AATGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGGGA	27160.8337	4489.9937	22670.8401	31650.8274	0.012170680	2231661.2040
M133_1685-1	-36.1522	15.6662	-4682	T343	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	TGCTACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTTCGTACGGG	3212.9930	3496.8021	-283.8092	6709.7951	0.007124368	450986.3828
M133_1685-1	-36.1522	15.6662	-4682	T344	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	111985.7275	44712.9357	67272.7918	156698.6633	0.015856130	7062613.9150
M133_1685-1	-36.1522	15.6662	-4682	T345	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCCCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGGA	4803.8751	854.5494	3949.3257	5658.4245	0.007948726	604357.8344
M133_1685-1	-36.1522	15.6662	-4682	T346	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	CTACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGC	33351.5442	3154.9142	30196.6300	36506.4584	0.021472195	1553243.3770
M133_1685-1	-36.1522	15.6662	-4682	T347	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCTT	195467.4194	23447.0726	172020.3468	218914.4921	0.007370812	26519116.1400
M133_1685-1	-36.1522	15.6662	-4682	T349	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	GATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCCAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	556.1282	112.8946	443.2336	669.0228	0.006217629	89443.7824
M133_1685-1	-36.1522	15.6662	-4682	T350	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	GCTACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGGA	133739.7502	5706.0032	128033.7470	139445.7533	0.010751685	12438957.7000
M133_1685-1	-36.1522	15.6662	-4682	T352	M133	ME1330/1685	70	60	80	G. siphonifera	S18F-S19F	45E-47F	TACATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGG	407.1014	55.2822	351.8192	462.3835	0.014962548	27208.0245
M133_1685-1	-36.1522	15.6662	-4682	T353	M133	ME1330/1685	70	60	80	G. siphonifera	S18F-S19F	45E-47F	TGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGG	4513.1662	523.9529	3989.2132	5037.1191	0.010814123	417339.9987
M133_1685-1	-36.1522	15.6662	-4682	T354	M133	ME1330/1685	70	60	80	G. siphonifera	S18F-S19F	45E-47F	ACATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTGGATTGGTGGTGAATTGTTGGCCCCGTCTAATACTGTCCTGTCTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTACTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGTAACCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGGA	4202.1297	350.6522	3851.4776	4552.7819	0.007904876	531587.0568
M133_1685-1	-36.1522	15.6662	-4682	T358	M133	ME1330/1685	70	60	80	G. siphonifera	S18F-S19F	45E-47F	CTACATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTGGATTGGTGGTGAATTGTTGGCCCCGTCTAATACTGTCCTGTCTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTACTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGTAACCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGG	306.5303	50.3116	256.2187	356.8418	0.008267774	37075.3131
M133_1685-1	-36.1522	15.6662	-4682	T444	M133	ME1330/1685	10	0	20	N. dutertrei	S18F-S19F	45E-47F	GATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	262.3471	42.3607	219.9864	304.7078	0.015575819	16843.2312
M133_1685-1	-36.1522	15.6662	-4682	T448	M133	ME1330/1685	10	0	20	G. glutinata	S18F-S19F	45E-47F	GTGCTACATGATTGTGGCAGTGAGCATCTTCAAATTTATTACATACGCCGCATACGTGAGTGAATTTTTTATTCCTTTTGC	9109.2389	550.9180	8558.3210	9660.1569	0.001045186	8715422.3540
M133_1685-1	-36.1522	15.6662	-4682	T452	M133	ME1330/1685	10	0	20	G. glutinata	S18F-S19F	45E-47F	TGCTAAATGATTGTGGCAGTGAGCATCTTCAAAATTTATTACATACGCCGCATACGTGAGTGATGGTTTTATTACTGTTACTTTGCGCA	13174.4194	1169.5692	12004.8502	14343.9886	0.000726005	18146463.9400
M133_1685-1	-36.1522	15.6662	-4682	T528	M133	ME1330/1685	90	80	100	G. glutinata	S18F-S19F	45E-47F	GCTAAATGATTGTTGCAGTGAGCATCTCAATTTTTTACCTAACACCGCACACGTGAGTGCATACTTGTATGTTACTTTACGCAGCGGTAAAGCCTGCTTCGAGAGCAAGTGGGTAATCAATTAGAAGTAACGATTTCCCAAATTAGCACACTTATATACGGCGTTTGTGCCCGGGTTTCACTTGTTGGAACTTTTGTGCGCGCAGATGTTTTTCCGTATGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	5232.5348	708.0510	4524.4837	5940.5858	0.000542522	9644841.6150
M133_1685-1	-36.1522	15.6662	-4682	T529	M133	ME1330/1685	90	80	100	G. glutinata	S18F-S19F	45E-47F	TGATTGTGGCAGTGAGCATCTTCAAATTTATTACATACGCCGCATACGTGAGTGATGGTT	6223.1434	1504.6032	4718.5402	7727.7466	0.000687372	9053530.4340
M133_1685-1	-36.1522	15.6662	-4682	T530	M133	ME1330/1685	90	80	100	G. glutinata	S18F-S19F	45E-47F	TGTTGCAGGTGAAGCATCTCAATTTTTTACCTAACACCGCACACGTGAGTGCATACTTGTATGTTACTTTA	195.7625	24.1950	171.5674	219.9575	0.000681955	287060.8356
M133_1685-1	-36.1522	15.6662	-4682	T531	M133	ME1330/1685	90	80	100	G. glutinata	S18F-S19F	45E-47F	TGCTACAATGATTGTTGCAGTGAGCATCTCAATTTTTTACCTAACACCGCACACGTGAGTGCATACTTGTATGTTACTTTACGCAGCGGTAAAGCCTGCTTCGAGAGCAAGTGGGTAATCAATTAGAAGTAACGATTTCCCAAATTAGCACACTTATATGCGGCGTTTGCGCCCGGGTATCACTTGTTGGTACTTCTGTGCGTGCAGATGTTTTTTCCGCATGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	1535.2117	36.3396	1498.8721	1571.5512	0.000833148	1842664.2790
M133_1685-1	-36.1522	15.6662	-4682	T549	M133	ME1330/1685	10	0	20	G. siphonifera	S18F-S19F	45E-47F	GATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGGA	463.7096	171.8342	291.8754	635.5437	0.023094585	20078.7138
M133_1685-1	-36.1522	15.6662	-4682	T550	M133	ME1330/1685	10	0	20	G. siphonifera	S18F-S19F	45E-47F	TGCTAATTGATTAGCGCAGTGCGCATACTTTCAATATGATACATTAGATTGGATTGTTCTGTTGAGCTCCATCTAATACTATCCGGCTTGAGAAGGCTGGGTAATCAATTGTAAGTGCTGGTTCCTCCTCCCGTTGAGCATTTTAATAATGGCCTCTCTACATCCCTAGCAATATGCCTAGTGCGATTGTAGTTGAGCCTTGCCATTTATGCAAGTGTCAATTCTCAGTGGGGACAGACATTTGATAATTCTTTGTCTCGTTCTTAACTAGGAATGCCTCGTACGGGA	13960.3190	1678.1628	12282.1562	15638.4818	0.004174293	3344355.5580
M133_1685-1	-36.1522	15.6662	-4682	T557	M133	ME1330/1685	10	0	20	N. dutertrei	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	204761.2061	6303.4091	198457.7970	211064.6151	0.012712691	16106834.3700
M133_1685-1	-36.1522	15.6662	-4682	T558	M133	ME1330/1685	10	0	20	N. dutertrei	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	742.2028	349.6761	392.5267	1091.8789	0.005506661	134782.7211
M133_1685-1	-36.1522	15.6662	-4682	T567	M133	ME1330/1685	10	0	20	G. glutinata	S18F-S19F	45E-47F	TGATTGTTGCAGTGAGCATCTCAATTTTTTACCTAACACCGCATACGTGAGTTAACTTTAGCTTCGGTTATTGTGTACTTTACGCAGCGGTAAAGCCTGCTTCGAGAGCAAGTGGGTAATCAATTAGAAG	1317.4848	269.5991	1047.8857	1587.0840	0.001258584	1046798.8920
M133_1685-1	-36.1522	15.6662	-4682	T568	M133	ME1330/1685	10	0	20	G. glutinata	S18F-S19F	45E-47F	TGATTGTTGCAGTGAGCATCTCAATTTTTTACCTAACACCGCACACGTGAGTGCATACTTGTATGTTACTTTACGCAGCGGTAAAGCCTGCTTCGAGAGCAAGTGGGTAATCAATTAGAAGTAACGATTTCCCAAATTAGCACACTTATATGCGGCGTTTGCGCCCGGG	786.9301	123.4866	663.4435	910.4167	0.000953704	825129.9418
M133_1685-1	-36.1522	15.6662	-4682	T569	M133	ME1330/1685	10	0	20	G. glutinata	S18F-S19F	45E-47F	GCATCTTCAAATTTATTACATACGCCGCATACGTGAGTGATGGTTTTATTACTTTTACTTTGCGCAACGGTGAAGCCTGAGTTGAGAGCTAGTGGGTAATCAATTAGAAGTTACGATCTCTTCGCATTAGATCTCTTATATACAGGGTTTATGCCCGGGTGTCACTTGTTCTAACCGCTGCGAGCTTATATGTTGTGAGTCTTCTACAACTTACATTTACGCTATGTGCAATTGTCGATGCGTGGAGAGGACAGACCATTGTTGATTTCTGGCCTCGCTCTTAATGAGGAATGCCGGATACG	11855.4759	2467.5215	9387.9544	14322.9973	0.001634733	7252239.2230
M133_1685-1	-36.1522	15.6662	-4682	T610	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	ACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	60429.8126	1844.2479	58585.5647	62274.0605	0.014716525	4106255.6830
M133_1685-1	-36.1522	15.6662	-4682	T613	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	2123.7024	185.2376	1938.4648	2308.9401	0.007661377	277195.9346
M133_1685-1	-36.1522	15.6662	-4682	T615	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACG	257.8685	116.3131	141.5554	374.1816	0.013015203	19812.8659
M133_1685-1	-36.1522	15.6662	-4682	T617	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	GATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	710.5308	234.1619	476.3689	944.6927	0.003715404	191239.1599
M133_1685-1	-36.1522	15.6662	-4682	T619	M133	ME1330/1685	70	60	80	N. dutertrei	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGT	5057.3324	313.4989	4743.8335	5370.8313	0.009418922	536933.2705
M133_1685-1	-36.1522	15.6662	-4682	T625	M133	ME1330/1685	70	60	80	G. glutinata	S18F-S19F	45E-47F	ATGATTGTTGCAGTGAGCATCTCAATTTTTTACCTAACACCGCACACGTGAGTTTTTG	3705.9603	290.6495	3415.3108	3996.6099	0.001786513	2074409.9130
M133_1685-1	-36.1522	15.6662	-4682	T626	M133	ME1330/1685	70	60	80	G. glutinata	S18F-S19F	45E-47F	ATGATTGTTGCAGTGAGCATCTCAATTTTTTACCTAACACCGCACACGTGAGTTTACATACTC	2260.1484	190.9435	2069.2049	2451.0919	0.002049561	1102747.4080
M133_1685-1	-36.1522	15.6662	-4682	T634	M133	ME1330/1685	30	20	40	T. sacculifer	S18F-S19F	45E-47F	GCTACAATGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGGG	1076.6038	279.5279	797.0759	1356.1318	0.017928669	60049.2906
M133_1685-1	-36.1522	15.6662	-4682	T655	M133	ME1330/1685	30	20	40	G. glutinata	S18F-S19F	45E-47F	TGATTGTTGCAGTGAGCATCTCAATTTTTTACCTAACACCGCACACGTGAGTGCATACTTGTATGTTACTTTACGCAGCGGTAAAGCCTGCTTCGAGAGCAAGTGGGTAATCAATTAGAAGTAACGATTTCCCAAATTAGCACACTTATATGCGGCGTTTGCGCCCGGGTACCACTTGTTGGTACTTCTGTGCGTGCAGATGTTTTTTCCGCATGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGGA	900.3779	88.2940	812.0840	988.6719	0.005895077	152733.8784
M133_1685-1	-36.1522	15.6662	-4682	T658	M133	ME1330/1685	30	20	40	G. glutinata	S18F-S19F	45E-47F	TGATTGTTGCAGTGAGCATCTCAATTTTTTACCTAACACCGCACACGTGAGTGCATACTTGTATGTTACTTTACGCAGCGGTAAAGCCTGCTTCGAGAGCAAGTGGGTAATCAATTAGAAGTAACGATTTCCCAAATTAGCACACTTATATGCGGCGTTTGCGCCCGGGTACCACTTGTTGGTACTTCTGTGCGTGCAGATGTTTTTTCC	2099.2029	503.0908	1596.1120	2602.2937	0.002055225	1021397.9670
M133_1685-1	-36.1522	15.6662	-4682	T664	M133	ME1330/1685	30	20	40	N. pachyderma	S18F-S19F	45E-47F	GCTACAATGATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGATTAATGGATCGTTTTTTATTAAGCGAGTCGTTTAATCGATGTTTTCTAATGTGCGCGGTAAAGCCTGCTTCGAGAGTAAGCGGGTAATCCATTGGAAGTAATGATTTCTCTATATCTAGTGCACAACTATGCACGGCACTCATTCCCGAGACGGCTAGTTTCGTCTTTTAGTGTGAATGTAGTGTTATTCAAACGTGCGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAACTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	969.7025	240.7756	728.9270	1210.4781	0.002598139	373229.6164
M133_1685-1	-36.1522	15.6662	-4682	T666	M133	ME1330/1685	30	20	40	N. pachyderma	S18F-S19F	45E-47F	ACATGATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGATTAATGGATCGCTTTTTATTAAGCGAGTCGTTTAATCGATGTTGTCTAATGTGCGCGGTAAAGCCTGCTTCGAGAGTAAGCGGGTAATCCATTGGAAGTAATGATTTCTCTATATCTAGTGCACAACTATGCACGGCACTCATTCCCGAGACGGCTAGTTTCGTCTTTTAGTGTGAATGTAGTGTTATTCAAACGTGCGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAACTGTTGGTCTCGGTCTTAACTAGGAATGCCTTCGTACGGGA	23277.2581	1671.8772	21605.3809	24949.1354	0.001280515	18178038.7300
M133_1685-1	-36.1522	15.6662	-4682	T668	M133	ME1330/1685	30	20	40	N. pachyderma	S18F-S19F	45E-47F	CTACAATGATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGATTAATGGATTGCTTTTTATTAAGCGAGTCGTTTAATCGATGTTGTCTAATGTGCGCGGTAAAGCCTGCTTCGAGAGTAAGCGGGTAATCCATTGGAAGTAATGATTTCTCTATATCTAGTGCACAACTATGCACGGCACTCATTCCCGAGACGGCTAGTTTCGTCTTTTAGTGTGAATGTAGTGTTATTCAAACGTGCGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAACTGTTGGTCTCGGTCTTAACTAGGAATGCCTTCGTACGGG	743.2942	184.2617	559.0324	927.5559	0.001585317	468861.5538
M133_1685-1	-36.1522	15.6662	-4682	T669	M133	ME1330/1685	30	20	40	N. pachyderma	S18F-S19F	45E-47F	ACATGATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGATTAATGGATCGTTTTTTATTAAGCGAGTCGTTTAATCGATGTTTTCTAATGTGCGCGGTAAAGCCTGCTTCGAGAGTAAGCGGGTAATCCATTGGAAGTAATGATTTCTCTATATCTAGTGCACAACTATGCACGGCACTCATTCCCGAGACGGCTAGTTTCGTCTTTTAGTGTGAATGTAGTGTTATTCAAACGTGCGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAACTGTTGGTCTCGGTCTTAACTAGGAATGCCTTCGTACGG	3002.3212	765.9781	2236.3431	3768.2993	0.001448260	2073053.4370
M133_1685-1	-36.1522	15.6662	-4682	T742	M133	ME1330/1685	50	40	60	T. sacculifer	S18F-S19F	45E-47F	AAATGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGGG	456.3154	121.2644	335.0510	577.5797	0.010390975	43914.5878
M133_1685-1	-36.1522	15.6662	-4682	T743	M133	ME1330/1685	50	40	60	T. sacculifer	S18F-S19F	45E-47F	ACAATGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGGG	15085.0128	691.3105	14393.7023	15776.3234	0.013811376	1092216.5170
M133_1685-1	-36.1522	15.6662	-4682	T746	M133	ME1330/1685	50	40	60	T. sacculifer	S18F-S19F	45E-47F	AATGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGG	66192.2363	8992.9107	57199.3256	75185.1471	0.013892590	4764571.2110
M133_1685-1	-36.1522	15.6662	-4682	T748	M133	ME1330/1685	50	40	60	T. sacculifer	S18F-S19F	45E-47F	CTACAATGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGGG	2661.3548	654.6521	2006.7026	3316.0069	0.003221528	826115.7571
M133_1685-1	-36.1522	15.6662	-4682	T749	M133	ME1330/1685	50	40	60	T. sacculifer	S18F-S19F	45E-47F	TGGCTAGCGCAGTGAGCATATTTCAACCGAAACATCGGTTGGCTGTTCTGATTGACCCCTTCTGGGTCTCTCTGTAACACTACCGATGATCCCTCCTCTGAAAAGAGAGGGTAAGCCGTTCGAAATTCTGGTAACGATTCCCCGTAGTTAAGCAAACTTAAACCATAGTGGTGTCAAACGGGTCCGCTTCCATCGGAAAAGATTCCTCCGGAAAAAGGCTTATGCAGGCATTTCACGTATGCTCCTATAAATTCCTGGTAGGGATAGTCTATTGTAACTGGTAGACTTGTCAACTAGGAATGCCTCGTACGGGA	6041.5957	761.3792	5280.2165	6802.9749	0.002291635	2636368.8700
M133_1685-1	-36.1522	15.6662	-4682	T761	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	GTGCTACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGG	35059.1655	3145.4428	31913.7226	38204.6082	0.020717674	1692234.5980
M133_1685-1	-36.1522	15.6662	-4682	T764	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	GATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTAT	101759.8165	9511.3478	92248.4686	111271.1643	0.009290564	10953028.9900
M133_1685-1	-36.1522	15.6662	-4682	T765	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	TACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGGA	2868.2419	223.9383	2644.3036	3092.1801	0.005418780	529315.0923
M133_1685-1	-36.1522	15.6662	-4682	T766	M133	ME1330/1685	50	40	60	N. dutertrei	S18F-S19F	45E-47F	GCTACAATGATCAGTACAGTGAGCATCTCAATATTATACACCGTATTAAGCGCTTAGTTGCGATTATTGGCTCATTATTGGGTCTTTTAATTGTATTTCTAATGCGCGCGGTAAAGCCTGCTTCGAGAGTAAGTGGGTAATCCATTGGAAGTAATGATTTCTCTTTTTATAGCACACCTATATACGGCATTCATTCCCGGGACGACTAGTTTCGTCTTTTTTGTGCGAATGTAATGTATTCTTTATCCGTATGTGCGATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	112.9697	10.6081	102.3616	123.5778	0.014022694	8056.2053
M133_1685-1	-36.1522	15.6662	-4682	T776	M133	ME1330/1685	50	40	60	G. glutinata	S18F-S19F	45E-47F	TGCTACATGATTGTTGCAGTGAGCATCTCAATTTTTTACCTAACACCGCACACGTGAGTAGCATACTTGTATGTTTACTTTACACAGCGGTAAAGCCTGCTTCGAGAGCGAGTGGGTAATC	1342.9186	594.7591	748.1596	1937.6777	0.001289010	1041822.0280
M133_1775-1	-34.5037	-18.8002	-3560	T730	M133	ME1330/1775	50	0	100	G. glutinata	S18F-S19F	45E-47F	TGATTGTTGCAGTGAGCATCTCAATTCTTTACCTTAACACCGCACACGTGAGTATTAACGTACTTTACGCAGCGGTAAAGCCTGCTTCGAGAGCAAGTGGGTAATCAATTAGAAGTAACGATTTCCCAAGTTAGCACACCTATATACGGCGTTTGTGCCCGGGTTTCACTTGTTGGAGCTTTTGTGCGCGCAGATGTTTCTTCCGTATGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACG	1794.2936	278.3195	1515.9740	2072.6131	0.001102338	1627715.6970
M133_56-1	-38.7685	-53.9655	-2495	T077	M133	ME1330/056	10	0	20	N. pachyderma	S14f1-S15r2	37F	NA	89633.0872	6310.4859	83322.6012	95943.5731	0.003738072	23978425.8400
MSM66/61-2	71.9999	-67.0004	-2330	T097	M133	ME1330/056	50	40	60	N. pachyderma	S14f1-S15r2	37F	NA	2435.3725	286.2567	2149.1159	2721.6292	0.001985054	1226854.4510
MSM66/61-2	71.9999	-67.0004	-2330	T100	M133	ME1330/056	50	40	60	N. pachyderma	S14f1-S15r2	37F	NA	48817.0685	2955.7004	45861.3681	51772.7689	0.002114453	23087329.4700
MSM66/61-2	71.9999	-67.0004	-2330	T103	M133	ME1330/056	50	40	60	N. pachyderma	S14f1-S15r2	37F	NA	97308.9498	7925.3796	89383.5701	105234.3294	0.002199850	44234359.5100
MSM66/61-2	71.9999	-67.0004	-2330	T104	M133	ME1330/056	50	40	60	N. pachyderma	S14f1-S15r2	37F	NA	41430.4708	367.1433	41063.3274	41797.6141	0.001246366	33241024.1100
MSM66/61-2	71.9999	-67.0004	-2330	T105	M133	ME1330/056	50	40	60	N. pachyderma	S14f1-S15r2	37F	NA	26212.9303	1815.9521	24396.9783	28028.8823	0.000982067	26691602.3800
MSM66/61-2	71.9999	-67.0004	-2330	T106	M133	ME1330/056	50	40	60	N. pachyderma	S14f1-S15r2	37F	NA	5089.4485	207.6576	4881.7910	5297.1061	0.003315486	1535053.6130
MSM66/61-2	71.9999	-67.0004	-2330	T077	M133	ME1330/056	10	0	20	N. pachyderma	S18F-S19F	45E-47F	CTACAATGATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGATTAATGGATCGCTTTTTATTAAGCGAGTCGTTTAATCGATGTTGTCTAATGTGCGCGGTAAAGCCTGCTTCGAGAGTAAGCGGGTAATCCATTGGAAGTAATGATTTCTCTATATCTAGTGCACAACTATGCACGGCACTCATTCCCGAGACGGCTAGTTTCGTCTTTTAGTGTGAATGTAGTGTTATTCAAACGTGCGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAACTGTTGGTCTCGGTCTTAACTAGGAATGCTCCGTACGG	49885.1268	1129.1501	48755.9767	51014.2768	0.003738072	13345147.9800
MSM66/61-2	71.9999	-67.0004	-2330	T097	M133	ME1330/056	50	40	60	N. pachyderma	S18F-S19F	45E-47F	CAATGATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGATTAATGGATCGCTTTTTATTAAGCGAGTCGTTTAATCGATGTTTTCTAATGTGCGCGGTAAAGCCTGCTTCGAGAGTAAGCGGGTAATCCATTGGAAGTAATGATTTCTCTATATCTAGTGCACAACTATGCACGGCACTCATTCCCGAGACGGCTAGTTTCGTCTTTTAGTGTGAATGTAGTGTTATTCAAACGTGCGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAACTGTTGGTCTCGGTCTTAACTAGGAATG	464.1777	138.4982	325.6795	602.6759	0.001985054	233836.2988
MSM66/61-2	71.9999	-67.0004	-2330	T100	M133	ME1330/056	50	40	60	N. pachyderma	S18F-S19F	45E-47F	TCCTGCCAACTGAAGGTGCATCTCCATATAATACAACGCTGTTTATCGCTTATGAGCCCCCTTTATGATCGTCTTTTATTCATCAAGTCGTTTCATCGATGTTTT	15481.1417	812.4369	14668.7048	16293.5786	0.002114453	7321582.9870
MSM66/61-2	71.9999	-67.0004	-2330	T103	M133	ME1330/056	50	40	60	N. pachyderma	S18F-S19F	45E-47F	GTGCTAAATGATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGATTAATGGATCGTTTTTTATTAAGCGAGTCGTTTAATCGATGTTTTCTAATGTGCGCGGTAAAGCCTGCTTCGAGAGTAAGCGGGTAATCCATTGGAAGTAATGATTTCTCTATATCTAGTGCACAACTATGCACGGCACTCATTCCCGAGACGGCTAGTTTCGTCTTTTAGTGTGAATGTAGTGTTATTCAAACGTGCGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAACTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	81587.0402	6514.9994	75072.0408	88102.0397	0.002199850	37087549.2500
MSM66/61-2	71.9999	-67.0004	-2330	T104	M133	ME1330/056	50	40	60	N. pachyderma	S18F-S19F	45E-47F	AATTATGTGTACAAGTGGAGCATCTCAATATAATGCAACGTTCTTTAGCGTCTTAGAACAGCGATCCCCATTAAATTTTTTTTTATTAAGCTTAGTCTTTTCATCTATTGTCTTCTAATGTATCGCGAGTGAATTCCTGCCTTCGACTAGTACATCGAGGTAATCCATTGTAATTATTGATTTCTCTATAT	15856.2002	368.6466	15487.5535	16224.8468	0.001246366	12721948.8900
MSM66/61-2	71.9999	-67.0004	-2330	T105	M133	ME1330/056	50	40	60	N. pachyderma	S18F-S19F	45E-47F	GTGCTACATGATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGATTAATGGATTGCTTTTTATTAAGCGAGTCGTTTAATCGATGTTTTCTAATGTGCGCGGTAAAGCCTGCTTCGAGAGTAAGCGGGTAATCCATTGGAAGTAATGATTTCTCTATATCTAGTGCACAACTATGCACGGCACTCATTCCCGAGACGGCTAGTTTCGTCTTTTAGTGTGAATGTAGTGTTATTCAAACGTGCGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAACTGTTGGTCTCGGTCTTAACTAGGAATGCCTTCGTACGGGA	11592.6285	531.3810	11061.2475	12124.0095	0.000982067	11804320.4800
MSM66/61-2	71.9999	-67.0004	-2330	T106	M133	ME1330/056	50	40	60	N. pachyderma	S18F-S19F	45E-47F	CTCAATATAATACACCGTCTTTAGCGCTTAGACGCGATTAATGGATCGCTTTTTATTAAGCGAGTCGTTTAATCGATGTTTTCTAATGTGCGCGGTAAAGCCTGCTTCGAGAGTAAGCGGGTAATCCATTGGAAGTAATGATTTCTCTATATCTAGTGCACAACTATGCACGGCACTCATTCCCGAGACGGCTAGTTTCGTCTTTTAGTGTGAATGTAGTGTTATTCAAACGTGCGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAACTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGGA	936.0445	90.0194	846.0252	1026.0639	0.003315486	282325.0014
MSM66/61-2	71.9999	-67.0004	-2330	T793	M133	ME1330/056	70	60	80	N. pachyderma	S18F-S19F	45E-47F	GCTACATGATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGATTAATGGATCGCTTTTTATTAAGCGAGTCGTTTAATCGATGTTTTCTAATGTGCGCGGTAAAGCCTGCTTCGAGAGTAAGCGGGTAATCCATTGGAAGTAATGATTTCTCTATATCTAGTGCACAACTATGCACGGCACTCATTCCCGAGACGGCTAGTTTCGTCTTTTAGTGTGAATGTAGTGTTATTCAAACGTGCGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAACTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	35018.7124	5681.5683	29337.1440	40700.2807	0.001602347	21854640.0300
MSM66/61-2	71.9999	-67.0004	-2330	T325	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S14f1-S15r2	37F	NA	385237.1012	52804.6402	332432.4610	438041.7415	0.003344592	115182102.3000
MSM66/61-2	71.9999	-67.0004	-2330	T328	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S14f1-S15r2	37F	NA	301270.3939	15214.3134	286056.0805	316484.7073	0.002512368	119914913.6000
MSM66/61-2	71.9999	-67.0004	-2330	T329	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S14f1-S15r2	37F	NA	171714.4084	1969.1332	169745.2752	173683.5415	0.003292142	52158870.0500
MSM66/61-2	71.9999	-67.0004	-2330	T330	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S14f1-S15r2	37F	NA	28493.6829	1765.6026	26728.0802	30259.2855	0.005499450	5181187.6780
MSM66/61-2	71.9999	-67.0004	-2330	T362	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S14f1-S15r2	37F	NA	201829.9357	29673.9756	172155.9601	231503.9113	0.004731608	42655679.7400
MSM66/61-2	71.9999	-67.0004	-2330	T363	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S14f1-S15r2	37F	NA	1821.5255	152.4218	1669.1037	1973.9472	0.002513121	724806.0278
MSM66/61-2	71.9999	-67.0004	-2330	T366	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S14f1-S15r2	37F	NA	100970.7865	10107.1082	90863.6784	111077.8947	0.003407909	29628371.8600
MSM66/61-2	71.9999	-67.0004	-2330	T367	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S14f1-S15r2	37F	NA	14935.0789	1052.7737	13882.3052	15987.8526	0.004262654	3503704.5350
MSM66/61-2	71.9999	-67.0004	-2330	T368	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S14f1-S15r2	37F	NA	3371.7415	444.5676	2927.1740	3816.3091	0.002552898	1320750.4490
MSM66/61-2	71.9999	-67.0004	-2330	T370	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S14f1-S15r2	37F	NA	5180.2026	996.0247	4184.1779	6176.2273	0.004437210	1167445.8250
MSM66/61-2	71.9999	-67.0004	-2330	T373	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S14f1-S15r2	37F	NA	823.0042	173.7130	649.2913	996.7172	0.001389226	592419.2387
MSM66/61-2	71.9999	-67.0004	-2330	T374	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S14f1-S15r2	37F	NA	463.1233	68.7326	394.3907	531.8559	0.002689703	172183.8077
MSM66/61-2	71.9999	-67.0004	-2330	T325	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S18F-S19F	45E-47F	TGCTACAATGATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGGTTATTGGCTTACTTTTTATTAAGTGAGTTTAATTAATCGATGATTTCTAATGTGCGCGGTAAAGCCTGCTTCGAGAGTAAGCGGGTAATCCATTGGAAGTAATGATTTCTCTATATCTAGTGCACAACTATGTACGGCATTCATTCCCGAGACGGCTAGTTTCGTCTTTTAGTGCGAATGTAGTGTTATTCAAACGTACGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAACTGTTGGTCTCGGTCTTAACTAGGAATGC	372600.7324	45094.5875	327506.1450	417695.3199	0.003344592	111403952.4000
MSM66/61-2	71.9999	-67.0004	-2330	T328	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGGTTATTGGCTTACTTTTTATT	347808.4391	35749.8163	312058.6228	383558.2554	0.002512368	138438491.7000
MSM66/61-2	71.9999	-67.0004	-2330	T329	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGGTTATTGGCTTACTTTTTCTTTAACGTTTAAGTGAGTTTAATTAATCGATGATTTCTAATGTGCGCGGTAAAGCCTGCTTCGAGAGTAAGCGGGTAATCCATTGGAAGTAATGATTTCTCTATATCTAGTGCACAACTATGTACGGCATTCATTCCCGAGACGGCTAGTTTCGTCTTTTAGTGCGAATGTAGTGTTATTCAAACGTACGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAACTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGGTACGGG	151277.1688	23997.9201	127279.2487	175275.0889	0.003292142	45950984.9100
MSM66/61-2	71.9999	-67.0004	-2330	T330	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S18F-S19F	45E-47F	CCCCTTTATGGTTGTCTTTTTTTATTTAACTCTTTGAGTCCTTGATTAATCATTTGCTTCTCAACGCCTGCTGCGAGCGTGCTCCGGTAATCAATTGGTAGTTTTTTTTTCTTTATTTCTTCTGGACAACTATGCACCTAAATAATTCCCGCCTCCGCTACGCTCCTCCTTTATTCTCATTTTATTCTTATACTACTATGCTTTCAACAGTCTATTCAATGTCTGGACATACTGTCAACACCTAATTCTTCTCATCTTTACTTGGA	15617.5079	421.1363	15196.3716	16038.6442	0.005499450	2839830.8520
MSM66/61-2	71.9999	-67.0004	-2330	T362	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGGTTATTGGCTTACTTTTTCTTTAACGT	93254.0141	12080.4114	81173.6027	105334.4255	0.004731608	19708738.1700
MSM66/61-2	71.9999	-67.0004	-2330	T363	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S18F-S19F	45E-47F	GATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGGTTATTGGCTTACTTTTTCTTTAACGTTTAAGTGAGTTTAATTAATCG	1554.5868	87.7620	1466.8248	1642.3488	0.002513121	618588.0412
MSM66/61-2	71.9999	-67.0004	-2330	T366	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S18F-S19F	45E-47F	TGCTAAATGATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGGTTATTGGCTTACTTTTTCTTTAACG	27914.6622	4916.3003	22998.3619	32830.9624	0.003407909	8191141.4120
MSM66/61-2	71.9999	-67.0004	-2330	T367	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S18F-S19F	45E-47F	GATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGGTTATTGGCTTACTTTT	3011.0687	608.6501	2402.4186	3619.7188	0.004262654	706383.6216
MSM66/61-2	71.9999	-67.0004	-2330	T368	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S18F-S19F	45E-47F	GTACAAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGGTTATTGCCATACCTTTTCCTTTACCTTTTAGTTAGTTTTATTTACC	297.7733	177.5052	120.2681	475.2785	0.002552898	116641.2809
MSM66/61-2	71.9999	-67.0004	-2330	T370	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S18F-S19F	45E-47F	NA	3005.7207	718.9022	2286.8185	3724.6229	0.004437210	677389.7391
MSM66/61-2	71.9999	-67.0004	-2330	T373	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S18F-S19F	45E-47F	TGATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGGTTATTGGCTTACTTTTTCTTTAACGTTTAAGTGAGTTTAATTAACCGATGATTTCTAATGTGCGCGGTAAAGCCTGCTTCGAGAGTAAGCGGGTAATCCATTGGAAGTAATGATTTCTCTATATCTAGTGCACAACTATGTACGGCATTCATTCCCGAGACGGCTAGTTTCGTCTTTTAGTGCGAATGTAGTGTTATTCAAACGTACGTGCAATTGTCAATTCATGGTGGGGACAGACCATTGTTAACTGTTGGTCTCGGTCTTAACTAGGAATGCCTCGTACGGG	490.2191	178.4216	311.7975	668.6406	0.001389226	352872.0732
MSM66/61-2	71.9999	-67.0004	-2330	T374	MSM66	GeoB22361-02	75	50	100	N. pachyderma	S18F-S19F	45E-47F	TAAATGATCAGTACAGTGAGCATCTCAATATAATACACCGTCTTTAGCGCTTAGACGCGGTAATTGCTATACCTTTTTCTTTAACGTT	373.0446	141.7901	231.2545	514.8347	0.002689703	138693.5997