Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 Eronen-Rasimus Eeva eeva.eronen-rasimus@syke.fi Asmala Eero eero.asmala@gtk.fi 0000-0002-9150-1227 Tamelander Tobias tobias.tamelander@helsinki.fi Kaartokallio Hermanni hermanni.kaartokallio@syke.fi 2021 Phytoplankton and bacteria incubations in part 2 (dissolved organic matter consumption) of the microcosm experiment, Gulf of Finland, Baltic Sea dataset https://doi.org/10.1594/PANGAEA.937741 2021-11-02T11:58:04 https://doi.org/10.1594/PANGAEA.937723 The data were collected from an experiment using phytoplankton cultures (Apocalathium malmogiense and Rhodomonas marina). The aim of the experiment was to study carbon cycling among phytoplankton and bacteria, and the effects on the dissolved organic matter (DOM) pool. Measured variables include phytoplankton and bacterial abundance, primary production, bacterial production and respiration, 14C-transfer from phytoplankton to DOM and bacteria, concentrations of particulate and dissolved organic carbon, nitrate, phosphate and chlorophyll a, and optical characteristics of dissolved organic matter. The experiment was conducted at Tvärminne Zoological Station, Hanko, Finland with non-axenic unialgal phytoplankton cultures and bacteria originating from the Baltic Sea. The experiment was conducted between Dec. 2017 and Apr. 2018. The experiment consisted of two parts, the DOM release experiment (part 1) and the DOM consumption experiment (part 2). Separate triplicate batch cultures of both phytoplankton species were grown for each experiment. In the DOM release experiment the cultures were grown for over 4 months and three day-long incubations (key point incubations, KPI's) were initiated on three occasions; the first KPI at early exponential growth phase and the second and third KPI's when the phytoplankton had grown more abundant. During each KPI and aliquot of the culture was inoculated with freshly collected sea water bacteria, and bacterial community composition was measured. This aliquot was then divided into two further aliquots; one was incubated with radioisotopes for productivity (primary and bacterial production) and 14C-flow analyses (production line) and one filtered through 0.8 µm for analysis of DOM optical properties. During the KPI's measurements were taken at 0, 4, 8 and 12 h. Nutrient concentrations (measured from non-filtered and 0.8 µm filtered samples) and concentration of dissolved organic carbon were measured only at 0 and 12 h. Concentrations of particulate organic carbon and nitrogen and chlorophyll a were measured only once for each KPI at the beginning of the incubation. In the DOM consumption experiments the cultures were grown to high abundance, after which the phytoplankton and most of the bacteria were filtered out. The filtrate was then inoculated with freshly collected sea water bacteria, after which it was incubated for 7 days. Bacterial abundance, production, respiration, and community composition, and concentration and optical properties of DOM were measured daily. The experimental design is explained in figure 1 of the associated publication. This data table contains measurements collected during the 7-day incubation of part 2 of the experiment (DOM consumption experiment). The measured variables are concentrations of nitrate, phosphate and dissolved organic carbon, incorporation rates of 3H-thymidine and 14C-leucine and bacterial production calculated based on these, abundance of high and low nucleic acid and total bacteria, flow cytometric side scatter of high and low nucleic acid bacteria, optical properties of DOM, and bacterial respiration rate. Daily respiration rate is calculated from continues oxygen measurement using optodes as explained in the associated publication. Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 Eronen-Rasimus Eeva eeva.eronen-rasimus@syke.fi Asmala Eero eero.asmala@gtk.fi 0000-0002-9150-1227 Tamelander Tobias tobias.tamelander@helsinki.fi Kaartokallio Hermanni hermanni.kaartokallio@syke.fi 2021 Contrasting patterns of carbon cycling and dissolved organic matter processing in two phytoplankton–bacteria communities journal article Biogeosciences 18(24) https://doi.org/10.5194/bg-18-6589-2021 6589-6616 23.249642 23.249642 59.844966 59.844966 23.249642 59.844966 2018-03-07T00:00:00 2018-04-11T00:00:00 not specified TZS_007_2018 59.844966 23.249642 Tvärminne Zoological Station, Finnland 2018-04-03T00:00:00 Bucket water sampling BUCKET Collection site of sea water bacteria. Apocalathium, DOM consumption experiment. TZS_008_2018 59.844966 23.249642 Tvärminne Zoological Station, Finnland 2018-03-05T00:00:00 Bucket water sampling BUCKET Collection site of sea water bacteria. Rhodomonas, DOM consumption experiment. 2228 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Event label Event Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 Event Identification ID Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 Event2 ID (Event2) Type of study Study type type Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 Study type Temperature, water Temp °C Temperature Θ http://dbpedia.org/resource/Temperature water http://purl.obolibrary.org/obo/CHEBI_15377 Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 Temp [°C] Species Species https://en.wikipedia.org/wiki/Species species Flow cytometry Accuri C6 Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 Species (Flow cytometry Accuri C6) Uniform resource locator/link to reference URL ref Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 WoRMS AphiaID URL ref (WoRMS AphiaID) Replicate Repl Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 Repl Incubation duration Inc dur days Time T http://dbpedia.org/resource/Time Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 Inc dur [days] DATE/TIME Date/Time http://en.wikipedia.org/wiki/ISO_8601 Time T http://dbpedia.org/resource/Time Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 experiment Date/Time (experiment) Nitrate [NO3]- µmol/l nitrate http://purl.obolibrary.org/obo/CHEBI_17632 Molar Concentration Autoanalyser (Thermo Scientific Aquakem 250) autoanalysers http://vocab.nerc.ac.uk/collection/L05/current/LAB04/ Chemical property sensors Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 [NO3]- [µmol/l] (Autoanalyser (Thermo Scientif...) Phosphate [PO4]3- µmol/l phosphate http://purl.obolibrary.org/obo/CHEBI_26020 Molar Concentration Autoanalyser (Thermo Scientific Aquakem 250) autoanalysers http://vocab.nerc.ac.uk/collection/L05/current/LAB04/ Chemical property sensors Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 [PO4]3- [µmol/l] (Autoanalyser (Thermo Scientif...) Carbon, organic, dissolved DOC µmol/l carbon C https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:27594 organic dissolved http://purl.obolibrary.org/obo/PATO_0001986 Molar Concentration Shimadzu TOC-VCPH total organic carbon analyzer Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 DOC [µmol/l] (Shimadzu TOC-VCPH total organ...) Thymidine incorporation rate Thym inc rate pmol/l/h Reaction Rate Liquid scintillation counter, Wallac 1414 LSC Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 Thym inc rate [pmol/l/h] (Liquid scintillation counter,...) Bacterial production BP pmol/l/h Reaction Rate Liquid scintillation counter, Wallac 1414 LSC Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 3H-thymidine BP [pmol/l/h] (3H-thymidine, Liquid scintill...) Leucine incorporation rate Leu inc rate pmol/l/h Reaction Rate Liquid scintillation counter, Wallac 1414 LSC Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 Leu inc rate [pmol/l/h] (Liquid scintillation counter,...) Bacterial production BP pmol/l/h Reaction Rate Liquid scintillation counter, Wallac 1414 LSC Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 14C-leucine BP [pmol/l/h] (14C-leucine, Liquid scintilla...) Absorption coefficient, 230 nm ac230 1/m Absorption Coefficient Spectrophotometer UV/VIS, Shimadzu, 2401 PC Chromatographs & spectrometers spectrophotometers http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 ac230 [1/m] (Spectrophotometer UV/VIS, Shi...) Absorption coefficient, 254 nm ac254 1/m Absorption Coefficient Spectrophotometer UV/VIS, Shimadzu, 2401 PC Chromatographs & spectrometers spectrophotometers http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 ac254 [1/m] (Spectrophotometer UV/VIS, Shi...) Absorption coefficient, 275 nm ac275 1/m Absorption Coefficient Spectrophotometer UV/VIS, Shimadzu, 2401 PC Chromatographs & spectrometers spectrophotometers http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 ac275 [1/m] (Spectrophotometer UV/VIS, Shi...) Absorption coefficient, 295 nm ac295 1/m Absorption Coefficient Spectrophotometer UV/VIS, Shimadzu, 2401 PC Chromatographs & spectrometers spectrophotometers http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 ac295 [1/m] (Spectrophotometer UV/VIS, Shi...) Absorption coefficient, 300 nm ac300 1/m Absorption Coefficient Spectrophotometer UV/VIS, Shimadzu, 2401 PC Chromatographs & spectrometers spectrophotometers http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 ac300 [1/m] (Spectrophotometer UV/VIS, Shi...) Absorption coefficient, 350 nm ac350 1/m Absorption Coefficient Spectrophotometer UV/VIS, Shimadzu, 2401 PC Chromatographs & spectrometers spectrophotometers http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 ac350 [1/m] (Spectrophotometer UV/VIS, Shi...) Absorption coefficient, 355 nm ac355 1/m Absorption Coefficient Spectrophotometer UV/VIS, Shimadzu, 2401 PC Chromatographs & spectrometers spectrophotometers http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 ac355 [1/m] (Spectrophotometer UV/VIS, Shi...) Absorption coefficient, 375 nm ac375 1/m Absorption Coefficient Spectrophotometer UV/VIS, Shimadzu, 2401 PC Chromatographs & spectrometers spectrophotometers http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 ac375 [1/m] (Spectrophotometer UV/VIS, Shi...) Absorption coefficient, 400 nm ac400 1/m Absorption Coefficient Spectrophotometer UV/VIS, Shimadzu, 2401 PC Chromatographs & spectrometers spectrophotometers http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 ac400 [1/m] (Spectrophotometer UV/VIS, Shi...) Absorption coefficient, 440 nm ac440 1/m Absorption Coefficient Spectrophotometer UV/VIS, Shimadzu, 2401 PC Chromatographs & spectrometers spectrophotometers http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 ac440 [1/m] (Spectrophotometer UV/VIS, Shi...) Spectral slope, 275-295 nm SpSl 275-295 nm 1/µm Inverse Length Spectrophotometer UV/VIS, Shimadzu, 2401 PC Chromatographs & spectrometers spectrophotometers http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 SpSl 275-295 nm [1/µm] (Spectrophotometer UV/VIS, Shi...) Spectral slope, 350-400 nm SpSl 350-400 nm 1/µm Inverse Length Spectrophotometer UV/VIS, Shimadzu, 2401 PC Chromatographs & spectrometers spectrophotometers http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 SpSl 350-400 nm [1/µm] (Spectrophotometer UV/VIS, Shi...) Slope ratio SR slope http://purl.obolibrary.org/obo/ENVO_00002000 ratio Spectrophotometer UV/VIS, Shimadzu, 2401 PC Chromatographs & spectrometers spectrophotometers http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 SR (Spectrophotometer UV/VIS, Shi...) Spectral slope, 300-650 nm SpSl 300-650 nm 1/µm Inverse Length Spectrophotometer UV/VIS, Shimadzu, 2401 PC Chromatographs & spectrometers spectrophotometers http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 SpSl 300-650 nm [1/µm] (Spectrophotometer UV/VIS, Shi...) Fluorescence, peak T F peak T RU https://doi.org/10.1016/0304-4203(95)00062-3 fluorescence http://purl.obolibrary.org/obo/PATO_0000018 Varian Cary Eclipse fluorometer (Agilent) Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 F peak T [RU] (Varian Cary Eclipse fluoromet...) Fluorescence, peak A F peak A RU https://doi.org/10.1016/0304-4203(95)00062-3 fluorescence http://purl.obolibrary.org/obo/PATO_0000018 Varian Cary Eclipse fluorometer (Agilent) Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 F peak A [RU] (Varian Cary Eclipse fluoromet...) Fluorescence, peak M F peak M RU https://doi.org/10.1016/0304-4203(95)00062-3 fluorescence http://purl.obolibrary.org/obo/PATO_0000018 Varian Cary Eclipse fluorometer (Agilent) Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 F peak M [RU] (Varian Cary Eclipse fluoromet...) Fluorescence, peak C F peak C RU https://doi.org/10.1016/0304-4203(95)00062-3 fluorescence http://purl.obolibrary.org/obo/PATO_0000018 Varian Cary Eclipse fluorometer (Agilent) Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 F peak C [RU] (Varian Cary Eclipse fluoromet...) Fluorescence index FI Varian Cary Eclipse fluorometer (Agilent) Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 FI (Varian Cary Eclipse fluoromet...) Humification index HIX Varian Cary Eclipse fluorometer (Agilent) Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 HIX (Varian Cary Eclipse fluoromet...) Biological index BIX Varian Cary Eclipse fluorometer (Agilent) Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 BIX (Varian Cary Eclipse fluoromet...) High nucleic acid bacteria HNA #/ml Flow cytometry Accuri C6 Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 HNA [#/ml] (Flow cytometry Accuri C6) Low nucleic acid bacteria LNA #/ml Flow cytometry Accuri C6 Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 LNA [#/ml] (Flow cytometry Accuri C6) Bacteria Bact #/ml Bacteria https://www.marinespecies.org/aphia.php?p=taxdetails&id=6 Flow cytometry Accuri C6 Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 Bact [#/ml] (Flow cytometry Accuri C6) High nucleic acid bacteria, cell size, side scatter HNA SSC cell size http://purl.obolibrary.org/obo/PATO_0000117 side scatter http://purl.obolibrary.org/obo/PATO_0002033 Flow cytometry Accuri C6 Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 geometric mean area HNA SSC (geometric mean area, Flow cyt...) Low nucleic acid bacteria, cell size, side scatter LNA SSC cell size http://purl.obolibrary.org/obo/PATO_0000117 side scatter http://purl.obolibrary.org/obo/PATO_0002033 Flow cytometry Accuri C6 Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 geometric mean area LNA SSC (geometric mean area, Flow cyt...) Specific ultraviolet absorbance normalized to DOC, 254 nm SUVA254 m**2/g normalized Shimadzu TOC-VCPH total organic carbon analyzer Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 SUVA254 [m**2/g] (Shimadzu TOC-VCPH total organ...) Respiration rate, carbon dioxide Resp CO2 µmol/l/day respiration rate http://www.oxfordreference.com/view/10.1093/oi/authority.20110803100415939?rskey=tF1NrR&result=2 carbon dioxide http://purl.obolibrary.org/obo/CHEBI_16526 Reaction Rate O2-meter (OXY-4 mini, PreSens) Elovaara Samu samu.elovaara@gmail.com 0000-0002-4586-0333 Resp CO2 [µmol/l/day] (O2-meter (OXY-4 mini, PreSens)) CurationLevelC Enhanced curation https://wiki.pangaea.de/wiki/Curation_levels citable 14C Bacteria bacterial production Baltic Sea CDOM DOM FDOM Laboratory experiment Laboratory strains Phytoplankton Primary production author31748 author64375 author67092 author73982 author85281 curlevel30 event2986728 event2986729 geocode1599 geocode1600 geocode1601 journal2146 license21 method10249 method10843 method13922 method7024 method8381 method9699 method9995 method9998 param120848 param125548 param125549 param129269 param140792 param140793 param140794 param143454 param145083 param149105 param149106 param167398 param167399 param167402 param167403 param167404 param167405 param167406 param167407 param167408 param167409 param184253 param184258 param185520 param23794 param25447 param25448 param25824 param2955 param30822 param53379 param53429 param53434 param53451 param54968 param7053 param7056 param717 param756 param758 param766 pi85281 ref110776 term1061861 term1073204 term1073482 term1073484 term1073490 term1073493 term1074060 term1074077 term1081264 term2299433 term2299583 term2606205 term2663825 term2687853 term38477 term38497 term38517 term38841 term38870 term39425 term40551 term43854 term43972 term43975 term44006 term44030 term44031 term44039 term44419 term47694 term47971 term48889 term49384 term49506 term50714 term51202 term52039 term67625 term68027 term69449 topotype1