de.pangaea.dataset937741 und de.pangaea.dataset937723 dataset PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact 2026-07-15T19:15:31Z https://doi.pangaea.de/10.1594/PANGAEA.937741 <gco:CharacterString>Phytoplankton and bacteria incubations in part 2 (dissolved organic matter consumption) of the microcosm experiment, Gulf of Finland, Baltic Sea</gco:CharacterString> 2021-11-02T11:58:04 publication https://doi.org/10.1594/PANGAEA.937741 Elovaara, Samu
samu.elovaara@gmail.com
https://orcid.org/0000-0002-4586-0333 information author Eronen-Rasimus, Eeva
eeva.eronen-rasimus@syke.fi
author Asmala, Eero
eero.asmala@gtk.fi
https://orcid.org/0000-0002-9150-1227 information author Tamelander, Tobias
tobias.tamelander@helsinki.fi
author Kaartokallio, Hermanni
hermanni.kaartokallio@syke.fi
author PANGAEA
info@pangaea.de
https://www.pangaea.de/ information publisher tableDigital The data were collected from an experiment using phytoplankton cultures (Apocalathium malmogiense and Rhodomonas marina). The aim of the experiment was to study carbon cycling among phytoplankton and bacteria, and the effects on the dissolved organic matter (DOM) pool. Measured variables include phytoplankton and bacterial abundance, primary production, bacterial production and respiration, 14C-transfer from phytoplankton to DOM and bacteria, concentrations of particulate and dissolved organic carbon, nitrate, phosphate and chlorophyll a, and optical characteristics of dissolved organic matter. The experiment was conducted at Tvärminne Zoological Station, Hanko, Finland with non-axenic unialgal phytoplankton cultures and bacteria originating from the Baltic Sea. The experiment was conducted between Dec. 2017 and Apr. 2018. The experiment consisted of two parts, the DOM release experiment (part 1) and the DOM consumption experiment (part 2). Separate triplicate batch cultures of both phytoplankton species were grown for each experiment. In the DOM release experiment the cultures were grown for over 4 months and three day-long incubations (key point incubations, KPI's) were initiated on three occasions; the first KPI at early exponential growth phase and the second and third KPI's when the phytoplankton had grown more abundant. During each KPI and aliquot of the culture was inoculated with freshly collected sea water bacteria, and bacterial community composition was measured. This aliquot was then divided into two further aliquots; one was incubated with radioisotopes for productivity (primary and bacterial production) and 14C-flow analyses (production line) and one filtered through 0.8 µm for analysis of DOM optical properties. During the KPI's measurements were taken at 0, 4, 8 and 12 h. Nutrient concentrations (measured from non-filtered and 0.8 µm filtered samples) and concentration of dissolved organic carbon were measured only at 0 and 12 h. Concentrations of particulate organic carbon and nitrogen and chlorophyll a were measured only once for each KPI at the beginning of the incubation. In the DOM consumption experiments the cultures were grown to high abundance, after which the phytoplankton and most of the bacteria were filtered out. The filtrate was then inoculated with freshly collected sea water bacteria, after which it was incubated for 7 days. Bacterial abundance, production, respiration, and community composition, and concentration and optical properties of DOM were measured daily. The experimental design is explained in figure 1 of the associated publication. This data table contains measurements collected during the 7-day incubation of part 2 of the experiment (DOM consumption experiment). The measured variables are concentrations of nitrate, phosphate and dissolved organic carbon, incorporation rates of 3H-thymidine and 14C-leucine and bacterial production calculated based on these, abundance of high and low nucleic acid and total bacteria, flow cytometric side scatter of high and low nucleic acid bacteria, optical properties of DOM, and bacterial respiration rate. Daily respiration rate is calculated from continues oxygen measurement using optodes as explained in the associated publication. completed Elovaara, Samu
samu.elovaara@gmail.com
https://orcid.org/0000-0002-4586-0333 information principalInvestigator 14C Bacteria bacterial production Baltic Sea CDOM DOM FDOM Laboratory experiment Laboratory strains Phytoplankton Primary production theme <gco:CharacterString>PANGAEA Keyword List</gco:CharacterString> 1995-01-01 creation PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact copyright copyright CC-BY-4.0: Creative Commons Attribution 4.0 International textTable und geoscientificInformation 23.249642 23.249642 59.844966 59.844966 2018-03-07T00:00:00 2018-04-11T00:00:00 Event label thematicClassification Identification thematicClassification Event2 Type of study thematicClassification Temperature, water physicalMeasurement °C °C Species thematicClassification Flow cytometry Accuri C6 Uniform resource locator/link to reference thematicClassification WoRMS AphiaID Replicate thematicClassification Incubation duration physicalMeasurement days days DATE/TIME thematicClassification experiment Nitrate physicalMeasurement Autoanalyser (Thermo Scientific Aquakem 250) µmol/l µmol/l Phosphate physicalMeasurement Autoanalyser (Thermo Scientific Aquakem 250) µmol/l µmol/l Carbon, organic, dissolved physicalMeasurement Shimadzu TOC-VCPH total organic carbon analyzer µmol/l µmol/l Thymidine incorporation rate physicalMeasurement Liquid scintillation counter, Wallac 1414 LSC pmol/l/h pmol/l/h Bacterial production physicalMeasurement Liquid scintillation counter, Wallac 1414 LSC; 3H-thymidine pmol/l/h pmol/l/h Leucine incorporation rate physicalMeasurement Liquid scintillation counter, Wallac 1414 LSC pmol/l/h pmol/l/h Bacterial production physicalMeasurement Liquid scintillation counter, Wallac 1414 LSC; 14C-leucine pmol/l/h pmol/l/h Absorption coefficient, 230 nm physicalMeasurement Spectrophotometer UV/VIS, Shimadzu, 2401 PC 1/m 1/m Absorption coefficient, 254 nm physicalMeasurement Spectrophotometer UV/VIS, Shimadzu, 2401 PC 1/m 1/m Absorption coefficient, 275 nm physicalMeasurement Spectrophotometer UV/VIS, Shimadzu, 2401 PC 1/m 1/m Absorption coefficient, 295 nm physicalMeasurement Spectrophotometer UV/VIS, Shimadzu, 2401 PC 1/m 1/m Absorption coefficient, 300 nm physicalMeasurement Spectrophotometer UV/VIS, Shimadzu, 2401 PC 1/m 1/m Absorption coefficient, 350 nm physicalMeasurement Spectrophotometer UV/VIS, Shimadzu, 2401 PC 1/m 1/m Absorption coefficient, 355 nm physicalMeasurement Spectrophotometer UV/VIS, Shimadzu, 2401 PC 1/m 1/m Absorption coefficient, 375 nm physicalMeasurement Spectrophotometer UV/VIS, Shimadzu, 2401 PC 1/m 1/m Absorption coefficient, 400 nm physicalMeasurement Spectrophotometer UV/VIS, Shimadzu, 2401 PC 1/m 1/m Absorption coefficient, 440 nm physicalMeasurement Spectrophotometer UV/VIS, Shimadzu, 2401 PC 1/m 1/m Spectral slope, 275-295 nm physicalMeasurement Spectrophotometer UV/VIS, Shimadzu, 2401 PC 1/µm 1/µm Spectral slope, 350-400 nm physicalMeasurement Spectrophotometer UV/VIS, Shimadzu, 2401 PC 1/µm 1/µm Slope ratio thematicClassification Spectrophotometer UV/VIS, Shimadzu, 2401 PC Spectral slope, 300-650 nm physicalMeasurement Spectrophotometer UV/VIS, Shimadzu, 2401 PC 1/µm 1/µm Fluorescence, peak T physicalMeasurement Varian Cary Eclipse fluorometer (Agilent) RU RU Fluorescence, peak A physicalMeasurement Varian Cary Eclipse fluorometer (Agilent) RU RU Fluorescence, peak M physicalMeasurement Varian Cary Eclipse fluorometer (Agilent) RU RU Fluorescence, peak C physicalMeasurement Varian Cary Eclipse fluorometer (Agilent) RU RU Fluorescence index thematicClassification Varian Cary Eclipse fluorometer (Agilent) Humification index thematicClassification Varian Cary Eclipse fluorometer (Agilent) Biological index thematicClassification Varian Cary Eclipse fluorometer (Agilent) High nucleic acid bacteria physicalMeasurement Flow cytometry Accuri C6 #/ml #/ml Low nucleic acid bacteria physicalMeasurement Flow cytometry Accuri C6 #/ml #/ml Bacteria physicalMeasurement Flow cytometry Accuri C6 #/ml #/ml High nucleic acid bacteria, cell size, side scatter thematicClassification Flow cytometry Accuri C6; geometric mean area Low nucleic acid bacteria, cell size, side scatter thematicClassification Flow cytometry Accuri C6; geometric mean area Specific ultraviolet absorbance normalized to DOC, 254 nm physicalMeasurement Shimadzu TOC-VCPH total organic carbon analyzer m**2/g m**2/g Respiration rate, carbon dioxide physicalMeasurement O2-meter (OXY-4 mini, PreSens) µmol/l/day µmol/l/day text/tab-separated-values unknown https://doi.pangaea.de/10.1594/PANGAEA.937741?format=textfile download dataset The data set was checked for completeness, correctness, and consistency of metainformation. Validity of used methods was checked and - if applicable - precision and range of data. EVENT LABEL: TZS_007_2018 * LATITUDE: 59.844966 * LONGITUDE: 23.249642 * DATE/TIME: 2018-04-03T00:00:00 * LOCATION: Tvärminne Zoological Station, Finnland * METHOD/DEVICE: Bucket water sampling Collection site of sea water bacteria. Apocalathium, DOM consumption experiment. 2018-04-03T00:00:00 EVENT LABEL: TZS_008_2018 * LATITUDE: 59.844966 * LONGITUDE: 23.249642 * DATE/TIME: 2018-03-05T00:00:00 * LOCATION: Tvärminne Zoological Station, Finnland * METHOD/DEVICE: Bucket water sampling Collection site of sea water bacteria. Rhodomonas, DOM consumption experiment. 2018-03-05T00:00:00 Supplement to <gco:CharacterString>Contrasting patterns of carbon cycling and dissolved organic matter processing in two phytoplankton–bacteria communities</gco:CharacterString> 2021-01-01 publication https://doi.org/10.5194/bg-18-6589-2021 Elovaara, Samu
samu.elovaara@gmail.com
https://orcid.org/0000-0002-4586-0333 information author Eronen-Rasimus, Eeva
eeva.eronen-rasimus@syke.fi
author Asmala, Eero
eero.asmala@gtk.fi
https://orcid.org/0000-0002-9150-1227 information author Tamelander, Tobias
tobias.tamelander@helsinki.fi
author Kaartokallio, Hermanni
hermanni.kaartokallio@syke.fi
author documentDigital Biogeosciences 18(24) 6589-6616