Adam Nicole nadam@geomar.de 0000-0002-4928-3924 Schlicht Stefanie Han Yuchen 0000-0003-3834-0310 Bechelany Mikhael Bachmann Julien 0000-0001-6480-6212 Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 2021 Hydrogen evolution activities of metagenomic fosmid clones derived from hydrothermal vent systems along the Mid-Atlantic Ridge dataset https://doi.org/10.1594/PANGAEA.937551 2021-10-26T08:18:58 Metagenomic fosmid libraries were constructed from hydrothermal chimneys and fluids and screened for hydrogenase activity by means of an activity-based screen. The hydrogen evolution activity of protein extracts from positively screened clones was determined by means of gas chromatography. For the most active clone (SP11F2) the hydrogen evolution activity was determined at different pH conditions and the stability of the respective enzyme ectract under different storage conditions was tested. The datasets contain hydrogen evolution activities of fosmid clones from the Sisters Peak, Nibelungen and Lilliput hydrothermal vent fields. Adam Nicole nadam@geomar.de 0000-0002-4928-3924 Schlicht Stefanie Han Yuchen 0000-0003-3834-0310 Bechelany Mikhael Bachmann Julien 0000-0001-6480-6212 Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 2020 Metagenomics Meets Electrochemistry: Utilizing the Huge Catalytic Potential From the Uncultured Microbial Majority for Energy-Storage journal article Frontiers in Bioengineering and Biotechnology 8(567) https://doi.org/10.3389/fbioe.2020.00567 -13.508833333332973 -12.37233329999998 -9.5438333333333 -4.804 -13.031333322221997 -7.548333333333333 2009-04-17T18:08:00 2009-04-30T11:50:00 -2980.0 -1519.0 not specified M78/2_274 -4.804 -12.3723333 -2980.0 2009-04-17T18:08:00 M78/2 M78/2 MARSUED5 MARSUED5 hdl:10013/epic.36797.d001 Port of Spain, Trinidad and Tobago 2009-04-01T00:00:00 2009-04-01 Rio de Janeiro, Brazil 2009-05-12T00:00:00 2009-05-12 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Seifert, Richard Seifert, Richard doi:10.2312/cr_m78_2 20090245 hdl:10013/epic.44784.d030 hdl:10013/epic.44803.d121 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Remote operated vehicle ROV M78/2_314 -8.2971666666667 -13.508833333333 -2886.0 2009-04-27T10:01:00 M78/2 M78/2 MARSUED5 MARSUED5 hdl:10013/epic.36797.d001 Port of Spain, Trinidad and Tobago 2009-04-01T00:00:00 2009-04-01 Rio de Janeiro, Brazil 2009-05-12T00:00:00 2009-05-12 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Seifert, Richard Seifert, Richard doi:10.2312/cr_m78_2 20090245 hdl:10013/epic.44784.d030 hdl:10013/epic.44803.d121 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Remote operated vehicle ROV M78/2_324 -9.5438333333333 -13.212833333333 -1519.0 2009-04-30T11:50:00 M78/2 M78/2 MARSUED5 MARSUED5 hdl:10013/epic.36797.d001 Port of Spain, Trinidad and Tobago 2009-04-01T00:00:00 2009-04-01 Rio de Janeiro, Brazil 2009-05-12T00:00:00 2009-05-12 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Seifert, Richard Seifert, Richard doi:10.2312/cr_m78_2 20090245 hdl:10013/epic.44784.d030 hdl:10013/epic.44803.d121 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Remote operated vehicle ROV 95 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Metagenomic fosmid libraries were constructed from the environmental sample and screened for hydrogen uptake activity (Adam and Perner 2018). The hydrogen evolution activity of the fosmid clones has been determined as described in the related publication. Event label Event Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 Event Area/locality Area Area http://dbpedia.org/resource/Area Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 Area Sample ID Sample ID Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 Sample ID Treatment: pH T:pH treatment Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 T:pH Treatment: temperature T:temp °C treatment Temperature Θ http://dbpedia.org/resource/Temperature Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 Storage temperature of the enzyme extract T:temp [°C] (Storage temperature of the en...) Treatment Treat treatment Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 Storage atmosphere of the enzyme extract Treat (Storage atmosphere of the enz...) Treatment: time after T:time after h treatment Time T http://dbpedia.org/resource/Time Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 T:time after [h] Hydrogen evolution activity H2 ev act nmol/min/mg Specific Activity http://search.ebscohost.com/login.aspx?direct=true&db=nlebk&AN=98508&site=ehost-live&ebv=EB&ppid=pp_610 Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 at 28°C H2 ev act [nmol/min/mg] (at 28°C) Hydrogen evolution activity, standard deviation H2 ev act std dev ± standard deviation std dev https://en.wikipedia.org/wiki/Standard_deviation Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 H2 ev act std dev [±] Hydrogen evolution activity H2 ev act % Dimensionless Ratio ψ Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 H2 ev act [%] Hydrogen evolution activity, standard deviation H2 ev act std dev ± standard deviation std dev https://en.wikipedia.org/wiki/Standard_deviation Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 H2 ev act std dev [±] Accession number, genetics Access no gen accession number Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 Access no gen Sample method Sample method Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 Sample method Comment Comment Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 Comment CurationLevelC Enhanced curation https://wiki.pangaea.de/wiki/Curation_levels citable Hydrogenase hydrothermal vent Metagenome geomar author73528 author75058 author79605 author81065 author81066 author81067 basis2 campaign3391645 campaign34047 curlevel30 event2621046 event2621085 event2621095 geocode1599 geocode1600 geocode1601 geocode8128 journal17100 license21 method11520 param105163 param106488 param153190 param15373 param1570 param16065 param16119 param174923 param192851 param192852 param505228 param96278 pi73528 ref107874 term242 term37764 term38742 term41013 term43770 term43794 term43972 term43975 term44032 term53736 term68002 term68003 term77733 term77900 topotype1