PANGAEA_937551
Hydrogen evolution activities of metagenomic fosmid clones derived from hydrothermal vent systems along the Mid-Atlantic Ridge
Adam, Nicole; Schlicht, Stefanie; Han, Yuchen; Bechelany, Mikhael; Bachmann, Julien; Perner, Mirjam
Hydrogen evolution activities of metagenomic fosmid clones derived from hydrothermal vent systems along the Mid-Atlantic Ridge
2021-10-26
PANGAEA
Dataset
https://doi.pangaea.de/10.1594/PANGAEA.937551
Investigator
Mirjam
Perner
mperner@geomar.de
Earth Science
Event label
Area/locality
Sample ID
Treatment: pH
Treatment: temperature
Treatment
Treatment: time after
Hydrogen evolution activity
Hydrogen evolution activity, standard deviation
Hydrogen evolution activity
Accession number, genetics
Sample method
Comment
geoscientificInformation
Hydrogenase
hydrothermal vent
M78/2
M78/2_274
M78/2_314
M78/2_324
Metagenome
Remote operated vehicle
Meteor (1986)
2009-04-17
2009-04-30
Complete
-9.5438333333333
-4.804
-13.508833333332973
-12.37233329999998
unrestricted
CC-BY-4.0: Creative Commons Attribution 4.0 International
English
PANGAEA
Data Publisher for Earth & Environmental Science
https://www.pangaea.de/
Data Center Contact
Michael
Diepenbroek
info@pangaea.de
Leobener Str.
Bremen
Bremen
28359
Germany
online
95 data points
text/tab-separated-values
Adam, Nicole; Schlicht, Stefanie; Han, Yuchen; Bechelany, Mikhael; Bachmann, Julien; Perner, Mirjam (2020): Metagenomics Meets Electrochemistry: Utilizing the Huge Catalytic Potential From the Uncultured Microbial Majority for Energy-Storage. Frontiers in Bioengineering and Biotechnology, 8(567), https://doi.org/10.3389/fbioe.2020.00567
Metagenomic fosmid libraries were constructed from hydrothermal chimneys and fluids and screened for hydrogenase activity by means of an activity-based screen. The hydrogen evolution activity of protein extracts from positively screened clones was determined by means of gas chromatography. For the most active clone (SP11F2) the hydrogen evolution activity was determined at different pH conditions and the stability of the respective enzyme ectract under different storage conditions was tested. The datasets contain hydrogen evolution activities of fosmid clones from the Sisters Peak, Nibelungen and Lilliput hydrothermal vent fields. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.937551"! ** Metagenomic fosmid libraries were constructed from the environmental sample and screened for hydrogen uptake activity (Adam and Perner 2018). The hydrogen evolution activity of the fosmid clones has been determined as described in the related publication.
https://doi.org/10.3389/fbioe.2020.00567
Metagenomics Meets Electrochemistry: Utilizing the Huge Catalytic Potential From the Uncultured Microbial Majority for Energy-Storage
https://en.wikipedia.org/wiki/RV_Meteor_(1986)
Meteor (1986)
https://hdl.handle.net/10013/epic.36797.d001
M78/2
DIF
9.4
2021-10-26
2026-04-29