Adam, Nicole; Schlicht, Stefanie; Han, Yuchen; Bechelany, Mikhael; Bachmann, Julien; Perner, Mirjam (2021): Hydrogen evolution activities of metagenomic fosmid clones derived from hydrothermal vent systems along the Mid-Atlantic Ridge [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.937551
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Abstract:
Metagenomic fosmid libraries were constructed from hydrothermal chimneys and fluids and screened for hydrogenase activity by means of an activity-based screen. The hydrogen evolution activity of protein extracts from positively screened clones was determined by means of gas chromatography. For the most active clone (SP11F2) the hydrogen evolution activity was determined at different pH conditions and the stability of the respective enzyme ectract under different storage conditions was tested. The datasets contain hydrogen evolution activities of fosmid clones from the Sisters Peak, Nibelungen and Lilliput hydrothermal vent fields.
Supplement to:
Adam, Nicole; Schlicht, Stefanie; Han, Yuchen; Bechelany, Mikhael; Bachmann, Julien; Perner, Mirjam (2020): Metagenomics Meets Electrochemistry: Utilizing the Huge Catalytic Potential From the Uncultured Microbial Majority for Energy-Storage. Frontiers in Bioengineering and Biotechnology, 8(567), https://doi.org/10.3389/fbioe.2020.00567
Coverage:
Median Latitude: -7.548333 * Median Longitude: -13.031333 * South-bound Latitude: -9.543833 * West-bound Longitude: -13.508833 * North-bound Latitude: -4.804000 * East-bound Longitude: -12.372333
Date/Time Start: 2009-04-17T18:08:00 * Date/Time End: 2009-04-30T11:50:00
Minimum Elevation: -2980.0 m * Maximum Elevation: -1519.0 m
Event(s):
M78/2_274 * Latitude: -4.804000 * Longitude: -12.372333 * Date/Time: 2009-04-17T18:08:00 * Elevation: -2980.0 m * Campaign: M78/2 (MARSUED5) * Basis: Meteor (1986) * Method/Device: Remote operated vehicle (ROV)
M78/2_314 * Latitude: -8.297167 * Longitude: -13.508833 * Date/Time: 2009-04-27T10:01:00 * Elevation: -2886.0 m * Campaign: M78/2 (MARSUED5) * Basis: Meteor (1986) * Method/Device: Remote operated vehicle (ROV)
M78/2_324 * Latitude: -9.543833 * Longitude: -13.212833 * Date/Time: 2009-04-30T11:50:00 * Elevation: -1519.0 m * Campaign: M78/2 (MARSUED5) * Basis: Meteor (1986) * Method/Device: Remote operated vehicle (ROV)
Comment:
Metagenomic fosmid libraries were constructed from the environmental sample and screened for hydrogen uptake activity (Adam and Perner 2018). The hydrogen evolution activity of the fosmid clones has been determined as described in the related publication.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Perner, Mirjam | |||
| 2 | Area/locality | Area | Perner, Mirjam | |||
| 3 | Sample ID | Sample ID | Perner, Mirjam | |||
| 4 | Treatment: pH | T:pH | Perner, Mirjam | |||
| 5 | Treatment: temperature | T:temp | °C | Perner, Mirjam | Storage temperature of the enzyme extract | |
| 6 | Treatment | Treat | Perner, Mirjam | Storage atmosphere of the enzyme extract | ||
| 7 | Treatment: time after | T:time after | h | Perner, Mirjam | ||
| 8 | Hydrogen evolution activity | H2 ev act | nmol/min/mg | Perner, Mirjam | at 28°C | |
| 9 | Hydrogen evolution activity, standard deviation | H2 ev act std dev | ± | Perner, Mirjam | ||
| 10 | Hydrogen evolution activity | H2 ev act | % | Perner, Mirjam | ||
| 11 | Hydrogen evolution activity, standard deviation | H2 ev act std dev | ± | Perner, Mirjam | ||
| 12 | Accession number, genetics | Access no gen | Perner, Mirjam | |||
| 13 | Sample method | Sample method | Perner, Mirjam | |||
| 14 | Comment | Comment | Perner, Mirjam |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
95 data points
Data
| 1 Event | 2 Area | 3 Sample ID | 4 T:pH | 5 T:temp [°C] (Storage temperature of the en...) | 6 Treat (Storage atmosphere of the enz...) | 7 T:time after [h] | 8 H2 ev act [nmol/min/mg] (at 28°C) | 9 H2 ev act std dev [±] | 10 H2 ev act [%] | 11 H2 ev act std dev [±] | 12 Access no gen | 13 Sample method | 14 Comment |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M78/2_324 | Lilliput hydrothermal field | Lilli33G1 | 153.22 | 8.65 | insdc:MG456606 | Diffuse hydrothermal fluids were collected from the Lilliput vent field using the KIPS (Kiel Pumping System, Garbe-Schönberg et al. 2006). | |||||||
| M78/2_314 | Nibelungen hydrothermal field | Nib22E5 | 13.12 | 1.37 | insdc:MG456605 | Hydrothermal fluids were collected from Dragon's throat crater using the KIPS (Kiel Pumping System, Garbe-Schönberg et al. 2006). | |||||||
| M78/2_274 | Sisters Peak hydrothermal field | SP11A2 | 280.53 | 15.36 | insdc:MG456603 | Beehive-like material from the Sisters Peak chimney structure was collected by means of the ROV. | |||||||
| M78/2_274 | Sisters Peak hydrothermal field | SP11F2 | 1002.64 | 86.16 | insdc:MG456604 | Beehive-like material from the Sisters Peak chimney structure was collected by means of the ROV. | |||||||
| M78/2_274 | Sisters Peak hydrothermal field | SP11F2 | 5 | 0.00 | 0.00 | Beehive-like material from the Sisters Peak chimney structure was collected by means of the ROV. | pH of the buffer used for the respective H2-evolution assay | ||||||
| M78/2_274 | Sisters Peak hydrothermal field | SP11F2 | 6 | 923.45 | 86.16 | Beehive-like material from the Sisters Peak chimney structure was collected by means of the ROV. | pH of the buffer used for the respective H2-evolution assay | ||||||
| M78/2_274 | Sisters Peak hydrothermal field | SP11F2 | 7 | 1073.02 | 114.63 | Beehive-like material from the Sisters Peak chimney structure was collected by means of the ROV. | pH of the buffer used for the respective H2-evolution assay | ||||||
| M78/2_274 | Sisters Peak hydrothermal field | SP11F2 | 8 | 1513.75 | 289.56 | Beehive-like material from the Sisters Peak chimney structure was collected by means of the ROV. | pH of the buffer used for the respective H2-evolution assay | ||||||
| M78/2_274 | Sisters Peak hydrothermal field | SP11F2 | 9 | 1128.36 | 155.92 | Beehive-like material from the Sisters Peak chimney structure was collected by means of the ROV. | pH of the buffer used for the respective H2-evolution assay | ||||||
| M78/2_274 | Sisters Peak hydrothermal field | SP11F2 | 4 | anoxic | 24 | 67.75 | 6.04 | Beehive-like material from the Sisters Peak chimney structure was collected by means of the ROV. | after storage of the enzyme extract for 24h under different conditions | ||||
| M78/2_274 | Sisters Peak hydrothermal field | SP11F2 | 4 | oxic | 24 | 44.22 | 2.16 | Beehive-like material from the Sisters Peak chimney structure was collected by means of the ROV. | after storage of the enzyme extract for 24h under different conditions | ||||
| M78/2_274 | Sisters Peak hydrothermal field | SP11F2 | 22 | anoxic | 24 | 24.20 | 3.00 | Beehive-like material from the Sisters Peak chimney structure was collected by means of the ROV. | after storage of the enzyme extract for 24h under different conditions | ||||
| M78/2_274 | Sisters Peak hydrothermal field | SP11F2 | 22 | oxic | 24 | 20.35 | 1.69 | Beehive-like material from the Sisters Peak chimney structure was collected by means of the ROV. | after storage of the enzyme extract for 24h under different conditions |