Citation:

PANGAEA.937532

Name: Seawater carbonate chemistry and growth and survival of juveniles of the striped venus clam Chamelea gallina
Published: 2021-10-25
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Other studied parameter or process; Pelagos; Phytoplankton; Pseudo-nitzschia australis; Pseudo-nitzschia fraudulenta; Single species

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Abstract:

This paper present the effects of ocean acidification on growth and domoic acid (DA) content of several strains of the toxic Pseudo-nitzschia australis and the non-toxic P. fraudulenta. Three strains of each species (plus two subclones of P. australis) were acclimated and grown in semi-continuous cultures at three pH levels: 8.07, 7.77, and 7.40, in order to simulate changes of seawater pH from present to plausible future levels. Our results showed that lowering pH from current level (8.07) to predicted pH level in 2100 (7.77) did not affect the mean growth rates of some of the P. australis strains (FR-PAU-17 and L3-100), but affected other strains either negatively (L3-30) or positively (L3.4). However, the growth rates significantly decreased with pH lowered to 7.40 (by 13% for L3-100, 43% for L3-30 and 16% for IFR-PAU-17 compared to the rates at pH 8.07). In contrast, growth rates of the non-toxic P. fraudulenta strains were not affected by pH changing from 8.07 to 7.40.

The P. australis strains produced DA at all pH levels tested, and the highest particulate DA concentration normalized to cell abundance (pDA) was found at pH 8.07. Total DA content (pDA and dissolved DA) was significantly higher at current pH (8.07) compared to pH (7.77), exept for one strain (L 3.4) where no difference was found. At lower pH levels 7.77 – 7.40, total DA content was similar, except for strains IFR-PAU-17 and L3-100 which had the lowest content at the pH 7.77. The diversity in the responses in growth and DA content highlights the inter- and intra-specific variation in Pseudo-nitzschia species in response to ocean acidification. When exploring environmental responses of Pseudo-nitzschia using cultured cells, not only strain-specific variation but also culturing history should be taken into consideration, as the light levels under which the subclones were cultured, afterwards affected both maximum growth rates and DA content.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Other studied parameter or process; Pelagos; Phytoplankton; Pseudo-nitzschia australis; Pseudo-nitzschia fraudulenta; Single species

Supplement to:

Ayache, Nour; Lundholm, Nina; Gai, Frederik; Hervé, F; Amzil, Zouher; Caruana, Amandine (2021): Impacts of ocean acidification on growth and toxin content of the marine diatoms Pseudo-nitzschia australis and P. fraudulenta. Marine Environmental Research, 169, 105380, https://doi.org/10.1016/j.marenvres.2021.105380

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-10-22.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Ayache, Nour		study
2	Species	Species		Ayache, Nour
3	Registration number of species	Reg spec no		Ayache, Nour
4	Uniform resource locator/link to reference	URL ref		Ayache, Nour		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	Ayache, Nour
6	Strain	Strain		Ayache, Nour
7	Treatment	Treat		Ayache, Nour
8	Growth rate	µ	1/day	Ayache, Nour		Maximum specific
9	Growth rate, standard deviation	µ std dev	±	Ayache, Nour		Maximum specific
10	Nitrate	[NO3]-	µmol/l	Ayache, Nour
11	Nitrate, standard deviation	NO3 std dev	±	Ayache, Nour
12	Phosphate	[PO4]3-	µmol/l	Ayache, Nour
13	Phosphate, standard deviation	[PO4]3- std dev	±	Ayache, Nour
14	Silicate	Si(OH)4	µmol/l	Ayache, Nour
15	Silicate, standard deviation	Si(OH)4 std dev	±	Ayache, Nour
16	Nitrate	[NO3]-	µmol/l	Ayache, Nour		measured at the beginning
17	Nitrate, standard deviation	NO3 std dev	±	Ayache, Nour		measured at the beginning
18	Phosphate	[PO4]3-	µmol/l	Ayache, Nour		measured at the beginning
19	Phosphate, standard deviation	[PO4]3- std dev	±	Ayache, Nour		measured at the beginning
20	Silicate	Si(OH)4	µmol/l	Ayache, Nour		measured at the beginning
21	Silicate, standard deviation	Si(OH)4 std dev	±	Ayache, Nour		measured at the beginning
22	Domoic acid per cell	DA/cell	pg/#	Ayache, Nour		Particulate
23	Domoic acid per cell, standard deviation	DA/cell std dev	±	Ayache, Nour		Particulate
24	Domoic acid per cell	DA/cell	pg/#	Ayache, Nour		Dissolved
25	Domoic acid per cell, standard deviation	DA/cell std dev	±	Ayache, Nour		Dissolved
26	Domoic acid per cell	DA/cell	pg/#	Ayache, Nour		Total
27	Domoic acid per cell, standard deviation	DA/cell std dev	±	Ayache, Nour		Total
28	pH	pH		Ayache, Nour		Seawater scale
29	Temperature, water	Temp	°C	Ayache, Nour
30	Salinity	Sal		Ayache, Nour
31	Carbon, inorganic, dissolved	DIC	µmol/l	Ayache, Nour
32	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Ayache, Nour
33	Bicarbonate	[HCO3]-	µmol/l	Ayache, Nour	Calculated using CO2SYS
34	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Ayache, Nour	Calculated using CO2SYS
35	Carbonate ion	[CO3]2-	µmol/l	Ayache, Nour	Calculated using CO2SYS
36	Carbonate ion, standard deviation	[CO3]2- std dev	±	Ayache, Nour	Calculated using CO2SYS
37	Carbon dioxide	CO2	µmol/l	Ayache, Nour	Calculated using CO2SYS
38	Carbon dioxide, standard deviation	CO2 std dev	±	Ayache, Nour	Calculated using CO2SYS
39	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Ayache, Nour	Calculated using CO2SYS
40	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Ayache, Nour	Calculated using CO2SYS
41	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
43	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

915 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp duration [days]	6 Strain	7 Treat	8 µ [1/day] (Maximum specific)	9 µ std dev [±] (Maximum specific)	10 [NO3]- [µmol/l]	11 NO3 std dev [±]	12 [PO4]3- [µmol/l]	13 [PO4]3- std dev [±]	14 Si(OH)4 [µmol/l]	15 Si(OH)4 std dev [±]	16 [NO3]- [µmol/l] (measured at the beginning)	17 NO3 std dev [±] (measured at the beginning)	18 [PO4]3- [µmol/l] (measured at the beginning)	19 [PO4]3- std dev [±] (measured at the beginning)	20 Si(OH)4 [µmol/l] (measured at the beginning)	21 Si(OH)4 std dev [±] (measured at the beginning)	22 DA/cell [pg/#] (Particulate)	23 DA/cell std dev [±] (Particulate)	24 DA/cell [pg/#] (Dissolved)	25 DA/cell std dev [±] (Dissolved)	26 DA/cell [pg/#] (Total)	27 DA/cell std dev [±] (Total)	28 pH (Seawater scale)	29 Temp [°C]	30 Sal	31 DIC [µmol/l]	32 DIC std dev [±]	33 [HCO3]- [µmol/l] (Calculated using CO2SYS)	34 [HCO3]- std dev [±] (Calculated using CO2SYS)	35 [CO3]2- [µmol/l] (Calculated using CO2SYS)	36 [CO3]2- std dev [±] (Calculated using CO2SYS)	37 CO2 [µmol/l] (Calculated using CO2SYS)	38 CO2 std dev [±] (Calculated using CO2SYS)	39 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	40 pCO2 std dev [±] (Calculated using CO2SYS)	41 CSC flag (Calculated using seacarb afte...)	42 pH (total scale, Calculated using...)	43 CO2 [µmol/kg] (Calculated using seacarb afte...)	44 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	45 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	46 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	47 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	48 DIC [µmol/kg] (Calculated using seacarb afte...)	49 AT [µmol/kg] (Calculated using seacarb afte...)	50 Omega Arg (Calculated using seacarb afte...)	51 Omega Cal (Calculated using seacarb afte...)
laboratory	Pseudo-nitzschia australis (phytoplankton)	246604	marinespecies.org	14	L 3.4	pH=8.07	1.43	0.04	881	27	32.1	0.1	149	1.8							0.06	0.02	0.04	0.010	0.10	0.030	8.07	16	32	2000	56	1828	44	158	10	13	2	362	49	27	8.08	12.99	351.32	352.57	1786.75	154.46	1954.19	2208.60	2.40	3.76
laboratory	Pseudo-nitzschia australis (phytoplankton)	246604	marinespecies.org	14	IFR-PAU-17	pH=8.07	1.67	0.08	510	25	32.9	0.5	147	0.6							2.49	0.15	1.04	0.070	3.53	0.220	8.07	16	32	2054	23	1878	12	163	10	14	1	371	23	27	8.08	13.34	360.77	362.06	1834.98	158.64	2006.95	2266.00	2.47	3.86
laboratory	Pseudo-nitzschia australis (phytoplankton)	246604	marinespecies.org	14	L 3-30	pH=8.07	2.47	0.04	670	29	33.7	0.6	154	1.8							0.37	0.06	0.02	0.010	0.39	0.070	8.07	16	32	2040	31	1865	27	162	4	14	0	369	9	27	8.08	13.25	358.33	359.62	1822.48	157.55	1993.28	2252.40	2.45	3.83
laboratory	Pseudo-nitzschia australis (phytoplankton)	246604	marinespecies.org	14	L 3-100	pH=8.07	1.77	0.06	578	32	32.7	1.1	157	0.3							1.64	0.09	0.08	0.010	1.72	0.100	8.07	16	32	1948	25	1781	21	154	3	13	0	351	8	27	8.08	12.65	342.20	343.43	1740.30	150.44	1903.38	2155.00	2.34	3.66
laboratory	Pseudo-nitzschia australis (phytoplankton)	246604	marinespecies.org	14	L 3.4	pH=7.77	1.65	0.03	785	14	33.1	0.3	136	0.3							0.07	0.01	0.03	0.010	0.10	0.020	7.77	16	32	2068	34	1955	32	85	1	28	0	771	9	27	7.78	27.71	749.70	752.39	1910.19	82.73	2020.63	2152.00	1.29	2.01
laboratory	Pseudo-nitzschia australis (phytoplankton)	246604	marinespecies.org	14	IFR-PAU-17	pH=7.77	1.83	0.11	693	50	34.3	0.4	140	1.3							0.25	0.08	0.18	0.050	0.43	0.130	7.77	16	32	2104	21	1989	18	86	2	29	1	785	19	27	7.78	28.20	762.85	765.58	1943.45	84.16	2055.81	2189.40	1.31	2.05
laboratory	Pseudo-nitzschia australis (phytoplankton)	246604	marinespecies.org	14	L 3-30	pH=7.77	2.33	0.04	611	38	33.3	0.4	151	0.9							0.15	0.01	0.01	0.003	0.16	0.013	7.77	16	32	2055	14	1943	12	84	1	28	1	766	13	27	7.78	27.54	744.99	747.65	1898.19	82.21	2007.93	2139.40	1.28	2.00
laboratory	Pseudo-nitzschia australis (phytoplankton)	246604	marinespecies.org	14	L 3-100	pH=7.77	1.93	0.21	662	18	34.4	0.6	151	1.1							0.63	0.17	0.04	0.004	0.67	0.174	7.77	16	32	2032	34	1921	24	83	7	28	2	758	66	27	7.78	27.23	736.81	739.45	1876.95	81.27	1985.46	2117.40	1.27	1.98
laboratory	Pseudo-nitzschia australis (phytoplankton)	246604	marinespecies.org	14	L 3.4	pH=7.40	1.72	0.05	723	41	33.7	0.2	149	1.1							0.07	0.01	0.03	0.010	0.10	0.020	7.40	16	32	2092	34	1988	32	37	1	68	0	1838	9	27	7.41	66.06	1787.27	1793.68	1942.15	35.87	2044.08	2065.60	0.56	0.87
laboratory	Pseudo-nitzschia australis (phytoplankton)	246604	marinespecies.org	14	IFR-PAU-17	pH=7.40	1.44	0.05	604	39	34.2	0.4	152	0.7							1.23	0.31	0.60	0.110	1.83	0.420	7.40	16	32	2103	27	1998	25	37	1	68	2	1848	42	27	7.41	66.46	1797.98	1804.43	1952.34	36.03	2054.83	2076.60	0.56	0.88
laboratory	Pseudo-nitzschia australis (phytoplankton)	246604	marinespecies.org	14	L 3-30	pH=7.40	1.72	0.03	565	22	34.1	1.1	151	0.7							0.13	0.01	0.02	0.003	0.15	0.013	7.40	16	32	1924	35	1828	26	34	3	62	6	1691	66	27	7.41	60.79	1644.75	1650.65	1786.17	32.97	1879.93	1904.20	0.51	0.80
laboratory	Pseudo-nitzschia australis (phytoplankton)	246604	marinespecies.org	14	L 3-100	pH=7.40	1.57	0.03	612	21	35.4	1.0	154	2.8							0.70	0.10	0.08	0.010	0.78	0.110	7.40	16	32	2004	32	1904	28	35	1	65	2	1761	64	27	7.41	63.31	1712.87	1719.01	1860.44	34.35	1958.10	1982.50	0.53	0.84
laboratory	Pseudo-nitzschia fraudulenta (phytoplankton)	246606	marinespecies.org	14	PNfra 167	pH=8.07	2.02	0.02	623	33	34.4	1.3	142	0.8													8.07	16	32	2049	46	1981	36	172	7	14	3	370	17	27	8.08	13.30	359.91	361.20	1830.52	158.25	2002.07	2262.20	2.46	3.85
laboratory	Pseudo-nitzschia fraudulenta (phytoplankton)	246606	marinespecies.org	14	PNfra 169	pH=8.07	1.79	0.13	666	21	34.1	0.4	142	1.3													8.07	16	32	2054	30	1877	27	163	2	14	0	371	6	27	8.08	13.34	360.82	362.11	1834.99	158.62	2006.95	2267.10	2.47	3.86
laboratory	Pseudo-nitzschia fraudulenta (phytoplankton)	246606	marinespecies.org	14	IFR-FRA-17	pH=8.07	1.76	0.05	579	16	32.9	0.7	136	5.0													8.07	16	32	2016	48	1843	29	160	17	13	2	364	62	27	8.08	13.09	354.15	355.41	1801.05	155.69	1969.82	2225.80	2.42	3.79
laboratory	Pseudo-nitzschia fraudulenta (phytoplankton)	246606	marinespecies.org	14	PNfra 167	pH=7.77	2.00	0.03	651	34	32.9	1.9	137	3.2													7.77	16	32	2168	42	2048	29	89	9	30	4	808	16	27	7.78	29.05	785.87	788.68	2002.56	86.74	2118.34	2252.20	1.35	2.11
laboratory	Pseudo-nitzschia fraudulenta (phytoplankton)	246606	marinespecies.org	14	PNfra 169	pH=7.77	1.72	0.07	675	11	33.8	0.6	137	1.9													7.77	16	32	2173	35	2054	31	89	3	30	1	810	29	27	7.78	29.12	787.85	790.67	2007.19	86.92	2123.23	2258.10	1.35	2.12
laboratory	Pseudo-nitzschia fraudulenta (phytoplankton)	246606	marinespecies.org	14	IFR-FRA-17	pH=7.77	1.82	0.06	646	33	32.5	0.8	132	0.7													7.77	16	32	2024	25	1913	15	83	8	28	3	755	70	27	7.78	27.11	733.56	736.19	1869.54	80.99	1977.64	2107.20	1.26	1.97
laboratory	Pseudo-nitzschia fraudulenta (phytoplankton)	246606	marinespecies.org	14	PNfra 167	pH=7.40	1.90	0.12	735	22	32.9	0.3	142	0.4													7.40	16	32	2164	36	2055	27	38	3	70	6	1901	57	27	7.41	68.39	1850.15	1856.78	2008.97	37.08	2114.44	2134.00	0.58	0.90
laboratory	Pseudo-nitzschia fraudulenta (phytoplankton)	246606	marinespecies.org	14	PNfra 169	pH=7.40	1.64	0.03	694	27	32.8	0.1	144	1.7													7.40	16	32	2157	30	2049	26	38	1	70	3	1895	72	27	7.41	68.15	1843.87	1850.48	2002.48	36.97	2107.60	2127.20	0.58	0.90
laboratory	Pseudo-nitzschia fraudulenta (phytoplankton)	246606	marinespecies.org	14	IFR-FRA-17	pH=7.40	1.72	0.08	785	53	32.6	0.9	140	3.8													7.40	16	32	2125	34	2019	25	37	3	69	6	1867	56	27	7.41	67.13	1816.16	1822.67	1972.77	36.42	2076.33	2096.20	0.57	0.89
laboratory						pH=8.07									881	15	35.1	0.8	160	3.5
laboratory						pH=7.77									939	36	36.6	1.2	159	4.7
laboratory						pH=7.40									944	40	36.2	0.9	155	1.8