Citation:

PANGAEA.937477

Name: Seawater carbonate chemistry and growth and survival of juveniles of the striped venus clam Chamelea gallina
Published: 2021-10-20
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Benthic animals; Benthos; Calcification/Dissolution; Chamelea gallina; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Growth/Morphology; Laboratory experiment; Mollusca; Mortality/Survival; North Atlantic; Single species; Temperate

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook TwitterShow Map Google Earth

Abstract:

Ocean acidification (OA) will decrease shellfish growth and survival, with ecological and economic consequences for fisheries and aquaculture. However, the high variability of results among experiments, and the lack of long-term studies, make it difficult to predict the effect that OA will have on bivalve species. We tested the long-term effect of high CO2 on growth, calcification rates, and survival of juveniles of the commercial bivalve species Chamelea gallina from Southern Portugal. The local high alkalinity of seawater probably buffered the negative effect of the pH drop, and after 75 days juveniles increased their growth and calcification rates with CO2. However, after 217 days, the situation reversed, bivalves under control conditions had the highest growth and calcification rates, while individuals under high CO2 presented negative calcification rates. The biometric variable that responded first was the width of the individuals, followed by the height and length of the shells. Survival was unaffected except for a mortality peak of juveniles under control and intermediate conditions as a consequence of a temperature drop. In the short term, C. gallina will increase their calcification rates to compensate for OA. However, in the long term, the additional energy expended will be translated into growth losses with negative repercussions for the fisheries and aquaculture. The cultivation of shellfish on high alkaline seawater should be further explored as a bioremediation measure to mitigate the negative effect of OA on shellfish aquaculture.

Keyword(s):

Animalia; Benthic animals; Benthos; Calcification/Dissolution; Chamelea gallina; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Growth/Morphology; Laboratory experiment; Mollusca; Mortality/Survival; North Atlantic; Single species; Temperate

Supplement to:

Sordo, Laura; Duarte, Catarina; Joaquim, S; Gaspar, Miguel B; Matias, D (2021): Long-term effects of high CO2 on growth and survival of juveniles of the striped venus clam Chamelea gallina: implications of seawater carbonate chemistry. Marine Biology, 168(8), https://doi.org/10.1007/s00227-021-03931-x

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 37.121600 * Longitude: -6.379900

Event(s):

EEMT_IPMA * Latitude: 37.121600 * Longitude: -6.379900 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-10-20.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Sordo, Laura		study
2	Species	Species		Sordo, Laura
3	Registration number of species	Reg spec no		Sordo, Laura
4	Uniform resource locator/link to reference	URL ref		Sordo, Laura		WoRMS Aphia ID
5	Treatment	Treat		Sordo, Laura
6	Month	Month		Sordo, Laura		Month/year
7	Duration	Duration		Sordo, Laura		Days
8	Temperature, water	Temp	°C	Sordo, Laura
9	Temperature, water, standard error	T std e	±	Sordo, Laura
10	Mortality	Mortality		Sordo, Laura		# of individuals
11	Day of experiment	DOE	day	Sordo, Laura
12	Length	l	mm	Sordo, Laura
13	Length, standard error	l std e	±	Sordo, Laura
14	Height	h	mm	Sordo, Laura
15	Height, standard error	h std e	±	Sordo, Laura
16	Width	w	mm	Sordo, Laura
17	Width, standard error	w std e	±	Sordo, Laura
18	Calcification rate of calcium carbonate	Calc rate CaCO3	µmol/g/h	Sordo, Laura
19	Salinity	Sal		Sordo, Laura
20	Salinity, standard error	Sal std e	±	Sordo, Laura
21	Temperature, water	Temp	°C	Sordo, Laura
22	Temperature, water, standard error	T std e	±	Sordo, Laura
23	pH	pH		Sordo, Laura	Potentiometric	NBS scale
24	pH, standard error	pH std e	±	Sordo, Laura	Potentiometric	NBS scale
25	Alkalinity, total	AT	µmol/kg	Sordo, Laura	Potentiometric titration
26	Alkalinity, total, standard error	AT std e	±	Sordo, Laura	Potentiometric titration
27	Bicarbonate ion	[HCO3]-	µmol/kg	Sordo, Laura	Calculated using CO2SYS
28	Bicarbonate ion, standard error	[HCO3]- std e	±	Sordo, Laura	Calculated using CO2SYS
29	Carbonate ion	[CO3]2-	µmol/kg	Sordo, Laura	Calculated using CO2SYS
30	Carbonate ion, standard error	[CO3]2- std e	±	Sordo, Laura	Calculated using CO2SYS
31	Carbon dioxide	CO2	µmol/kg	Sordo, Laura	Calculated using CO2SYS
32	Carbon dioxide, standard error	CO2 std e	±	Sordo, Laura	Calculated using CO2SYS
33	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Sordo, Laura	Calculated using CO2SYS
34	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Sordo, Laura	Calculated using CO2SYS
35	Aragonite saturation state	Omega Arg		Sordo, Laura	Calculated using CO2SYS
36	Aragonite saturation state, standard error	Omega Arg std e	±	Sordo, Laura	Calculated using CO2SYS
37	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
39	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2817 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Treat	6 Month (Month/year)	7 Duration (Days)	8 Temp [°C]	9 T std e [±]	10 Mortality (# of individuals)	11 DOE [day]	12 l [mm]	13 l std e [±]	14 h [mm]	15 h std e [±]	16 w [mm]	17 w std e [±]	18 Calc rate CaCO3 [µmol/g/h]	19 Sal	21 Temp [°C]	23 pH (NBS scale, Potentiometric)	24 pH std e [±] (NBS scale, Potentiometric)	25 AT [µmol/kg] (Potentiometric titration)	26 AT std e [±] (Potentiometric titration)	27 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	28 [HCO3]- std e [±] (Calculated using CO2SYS)	29 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	30 [CO3]2- std e [±] (Calculated using CO2SYS)	31 CO2 [µmol/kg] (Calculated using CO2SYS)	32 CO2 std e [±] (Calculated using CO2SYS)	33 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	34 pCO2water_SST_wet std e [±] (Calculated using CO2SYS)	35 Omega Arg (Calculated using CO2SYS)	36 Omega Arg std e [±] (Calculated using CO2SYS)	37 CSC flag (Calculated using seacarb afte...)	38 pH (total scale, Calculated using...)	39 CO2 [µmol/kg] (Calculated using seacarb afte...)	40 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	41 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	42 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	43 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	44 DIC [µmol/kg] (Calculated using seacarb afte...)	45 Omega Arg (Calculated using seacarb afte...)	46 Omega Cal (Calculated using seacarb afte...)
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2	Nov-19	0-15	18.4963	0.7492	0									33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2	Dec-19	16-46	18.0072	0.4864	20									33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2	Jan-20	47-77	16.7587	0.5575	327									33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2	Feb-20	78-106	18.5684	0.2805	90									33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2	Mar-20	107-137	18.8638	0.5528	11									33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2	Apr-20	138-167	19.6569	0.6065	2									33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2	May-20	168-198	21.1136	0.9382	4									33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2	Jun-20	199-217	22.0733	0.5614	1									33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2	Nov-19	0-15	18.4963	0.7492	0									33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2	Dec-19	16-46	18.0072	0.4864	33									33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2	Jan-20	47-77	16.7587	0.5575	336									33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2	Feb-20	78-106	18.5684	0.2805	73									33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2	Mar-20	107-137	18.8638	0.5528	14									33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2	Apr-20	138-167	19.6569	0.6065	6									33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2	May-20	168-198	21.1136	0.9382	15									33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2	Jun-20	199-217	22.0733	0.5614	5									33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2	Nov-19	0-15	18.4963	0.7492	0									33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2	Dec-19	16-46	18.0072	0.4864	0									33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2	Jan-20	47-77	16.7587	0.5575	31									33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2	Feb-20	78-106	18.5684	0.2805	40									33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2	Mar-20	107-137	18.8638	0.5528	6									33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2	Apr-20	138-167	19.6569	0.6065	6									33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2	May-20	168-198	21.1136	0.9382	5									33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2	Jun-20	199-217	22.0733	0.5614	0									33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						0	7.0913	0.6406	6.1725	0.5470	3.3824	0.3329		33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						25	7.1483	0.6146	6.4108	0.5347	3.3424	0.3117		33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						45	7.1459	0.6061	6.4460	0.5407	3.3504	0.3175		33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						62	7.1698	0.5614	6.4389	0.4978	3.3685	0.2954		33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						83	7.1351	0.5852	6.4202	0.5238	3.3794	0.3014		33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						105	7.0635	0.5427	6.3852	0.4839	3.3666	0.2897		33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						153	8.0875	1.0811	7.3051	0.9500	4.0033	0.6288		33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						217	8.9829	1.5007	8.2613	1.1820	4.5363	0.7618		33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						0	7.0563	0.6125	6.1343	0.5908	3.3136	0.3373		33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						25	7.1176	0.6216	6.3923	0.5239	3.2913	0.3337		33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						45	7.1520	0.6289	6.4008	0.5330	3.2957	0.3364		33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						62	7.0936	0.7363	6.3882	0.5680	3.3592	0.5724		33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						83	7.0301	0.6271	6.3215	0.5373	3.3117	0.3270		33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						105	7.0270	0.6155	6.3298	0.5343	3.2989	0.3269		33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						153	7.6232	0.9326	6.8879	0.8133	3.7232	0.5514		33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						217	8.5331	1.3734	7.7673	1.2221	4.2139	0.8079		33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						0	7.1092	0.6854	6.2033	0.6106	3.3647	0.4369		33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						25	7.2390	0.6807	6.5207	0.5855	3.4071	0.3548		33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						45	7.2673	0.6801	6.5355	0.6078	3.4218	0.3592		33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						62	7.2835	0.6856	6.5776	0.5956	3.4527	0.3498		33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						83	7.2693	0.6820	6.5744	0.5978	3.4714	0.3607		33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						105	7.4808	0.8255	6.7751	0.7167	3.5785	0.4431		33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						153	8.4504	1.2927	7.6580	1.1354	4.1567	0.7067		33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						217	8.9321	1.4434	8.1848	1.2958	4.4448	0.8244		33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						75							-0.3834	33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						75							-0.8936	33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						75							-0.4958	33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						113							0.1662	33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						113							0.1650	33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						113							0.1714	33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						196							2.9454	33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						196							3.0015	33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Control CO2						196							2.1630	33.93	23.0	8.28	0.001	3631.11	16.38	2756.83	14.51	383.21	1.14	14.39	0.11	480.13	3.83	6.07	0.018	26	8.13	14.84	496.36	497.99	2764.58	378.68	3158.1	5.96	9.09
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						75							0.4464	33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						75							0.4468	33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						75							0.3201	33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						113							0.1714	33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						113							0.3089	33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						113							-0.1027	33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						196							3.1202	33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						196							3.9444	33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	Intermediate CO2						196							3.1079	33.92	23.2	8.09	0.002	3495.18	4.47	2904.96	7.06	257.55	1.00	23.84	0.20	799.91	5.83	4.08	0.016	26	7.94	24.03	808.13	810.78	2901.29	258.38	3183.7	4.07	6.20
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						75							0.8891	33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						75							0.3644	33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						75							1.0218	33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						113							4.2418	33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						113							5.3472	33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						113							5.6191	33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						196							-1.3373	33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						196							-0.6358	33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13
laboratory	Chamelea gallina (mollusk)	141907	marinespecies.org	High CO2						196							-0.0706	33.93	23.1	7.75	0.008	3494.00	2.73	3195.93	7.55	130.09	2.42	57.10	1.35	1872.73	12.75	2.06	0.038	26	7.61	57.71	1935.83	1942.19	3194.76	130.23	3382.7	2.05	3.13